Gene Dmel\Nurf-38

60C-60C

60D5-60D6

(determined by in situ hybridisation)

(Spradling et al., 1999)

60C-60C

(BDGP Project Members, 1994-1999)

60D5-60D6

(Beaton, 1997.12.27)

60D5-60D6

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Nurf-38 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Nurf-38-RA

FBtr0072343

NM_079125

1181

338

Nurf-38-RB

FBtr0273253

1135

290

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Nurf-38-PA

FBpp0072250

37.9

338

7.01

NP_523849

Nurf-38-PB

FBpp0271761

32.6

290

5.41

Additional Polypeptide Data & Comments

Reported size (kD)

290 (aa); 40 (kD observed); 33 (kD predicted)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0016687

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Inorganic pyrophosphatase (IPR008162)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O77460

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE37074

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

1684

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

nucleus cytoplasm

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI106806

External Data & Images

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0016687

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0016687

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Nurf-38^k16102

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Nurf-38	Class	Mutagen	Stocks	Known lesion
Nurf-38¹			0	--
Nurf-38^k16102		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Nurf-38	Class	Mutagen	Stocks	Known lesion
Nurf-38^GD1625		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)Px2

Useful duplication

Dp(2;3)Me³

Disrupted in

Df(2R)Px2

Not disrupted in

Df(2R)bw^VDe2LPx^KR

(Spradling et al., 1999, Beaton, 1997.12.27)

In(2LR)Px⁴

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1625}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}Nurf-38^k16102

P{lacW}l(2)SH2046^SH2046

Related Comments

Please look at the allele reports for the complete phenotype data

Nucleosome remodelling factor is composed of at least four polypeptides that act in concert with the Trl transcription factor at the Hsp70 promoter. Nucleosome remodelling factor acts directly on the nucleosome to perturb its structure.

(Tsukiyama and Wu, 1995)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

inorganic diphosphatase activity

inferred from direct assay

non-traceable author statement

(Spradling et al., 1999)

non-traceable author statement

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity with EMBL:AF108211

(FlyBase, 1992-)

magnesium ion binding

inferred from electronic annotation with InterPro:IPR008162

Biological Process

CV term

Evidence

References

chromatin remodeling

inferred from direct assay

ecdysone receptor-mediated signaling pathway

inferred from genetic interaction with FLYBASE:EcR; FB:FBgn0000546

(Badenhorst et al., 2005)

nucleosome mobilization

inferred from direct assay

(Hamiche et al., 1999)

phosphate metabolic process

inferred from electronic annotation with InterPro:IPR008162

(FlyBase Curators et al., 2004-)

transcription

inferred from direct assay

(Mizuguchi et al., 2001)

Cellular Component

CV term

Evidence

References

NURF complex

inferred from direct assay AND inferred from physical interaction with FLYBASE:Caf1; FB:FBgn0015610 AND inferred from physical interaction with FLYBASE:Iswi; FB:FBgn0011604

non-traceable author statement

(Swiss-Prot Project Members, 1998.12.15)

non-traceable author statement

traceable author statement

(Badenhorst et al., 2002)

cytoplasm

inferred from direct assay

(Swiss-Prot Project Members, 1998.12.15)

non-traceable author statement

nucleus

inferred from direct assay

(Swiss-Prot Project Members, 1998.12.15)

non-traceable author statement

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Nurf-38 allele

Gene

References

unspecified

EcR

(Badenhorst et al., 2005)

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63494

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521845

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11243

Dere\GG19918

Dgri\GH21893

Dmoj\GI20392

Dper\GL10109

Dpse\GA25082

Dsec\GM11820

Dsim\GD24942

Dvir\GJ22117

Dwil\GK19471

Dyak\GE11442

Curated drosophilid orthologs

Dpse\GA18319

y¹ w^67c23; P{lacW}Nurf-38^k16102/CyO

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000027279
Anopheles gambiae (Ensembl)
ENSBTAP00000010311
Bos taurus (Ensembl)
ENSCAFP00000016012
Canis familiaris (Ensembl)
ENSCINP00000004772
Ciona intestinalis (Ensembl)
NEWSINFRUP00000142480
Takifugu rubripes (Ensembl)
ENSGALP00000017202
Gallus gallus (Ensembl)
ENSP00000343885
Homo sapiens (Ensembl)
ENSMMUP00000022376
Macaca mulatta (Ensembl)
ENSMODP00000025811
Monodelphis domestica (Ensembl)
ENSPTRP00000004481
Pan troglodytes (Ensembl)
ENSRNOP00000016195
Rattus norvegicus (Ensembl)
GSTENP00010127001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000013520
Xenopus tropicalis (Ensembl)
Q6ZGJ8
Oryza sativa (Gramene)
AT5G09650.1
Arabidopsis thaliana (Arabidopsis)
WBGene00026305
Caenorhabditis briggsae (Wormbase)
WBGene00008149
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-050417-376
Danio rerio (Zfin)
ZDB-GENE-050417-375
Danio rerio (Zfin)
DDB0185935
Dictyostelium discoideum (Dictybase)
MGI:1922026
Mus musculus (Informatics)
YBR011C
Saccharomyces cerevisiae (Yeastgenome)
SPAC23C11.05
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v3200

w¹¹¹⁸; P{GD1625}v3200/TM3

Bloomington

12206

Genomic Clones ( 1 )

BACR11C07

cDNA Clones ( 64 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE37074

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0016687

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0016687

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Nurf-38 CG4634

(Wang, 1999.11)

Source for database merge of

Source for merge of: Nurf-38 l(2)k16102

(Spradling et al., 1999)

Additional comments

Comments About Role

NURF, a protein complex of four subunits Caf1, Nurf-38, E(bx) and Iswi, is able to facilitate transcription mediated by a Scer\GAL4 derivative carrying a constitutively activating region from Hsf. Once nucleosome remodelling by the DNA binding factor is accomplished a high level of NURF activity is not continuously required for recruitment of the general transcription machinery and transcription initiation. NURF is able to assist gene activation in a chromatin context: remodel nucleosomes for gene activation in chromatin.

(Mizuguchi et al., 1997)

Nurf-38 may serve a structural or regulatory role in the nucleosome remodeling factor (NURF) complex.