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Gene Dmel\wun

General Information
Symbol	Dmel\wun	Species	D. melanogaster
Name	wunen	Annotation symbol	CG8804
Feature type	protein_coding_gene	FlyBase ID	FBgn0016078
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	45D3-45D4	Sequence location	2R:5,288,052..5,300,965 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene wunen is referred to in FlyBase by the symbol wun (CG8804, FBgn0016078). It has the cytological map location 45D3-45D4. Its sequence location is 2R:5288052..5300965. Its molecular function is described as: phosphoric monoester hydrolase activity; phosphatidate phosphatase activity; protein homodimerization activity. It is involved in the biological processes: germ cell migration; dephosphorylation; pole cell migration; germ cell programmed cell death; germ cell repulsion; embryonic pattern specification. 27 alleles are reported. The phenotypes of these alleles are annotated with: germ cell; embryonic proventriculus; embryonic digestive system; synapse; bouton; anterior fascicle. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45D3-45D4   Limits computationally determined from genome sequence between P{lacW}Pknk11209 and P{lacW}wunk09507&P{lacW}l(2)k12402k12402
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45D4-45D5 (determined by in situ hybridisation)   (Spradling et al., 1999) 45D4-45D5 (determined by in situ hybridisation)   (Zhang et al., 1996) 45D4-45D5   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Zhang et al., 1996)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: l(2)03659? l(2)k10201+ wun- (Zhang et al., 1997) Overall orientation not stated: wun2? wun? (Hayden, 1999.3.3) Overall orientation not stated: l(2)k10201+ wun- wun2+ (Starz-Gaiano, 2000.3.10) Overall orientation not stated: wun- wun2+ (Starz-Gaiano et al., 2001)
Gene Model & Products
Please see the GBrowse view of Dmel\wun for information on other features
Comments on Gene Model
	EST data suggest additional 3' exon(s)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wun-RA FBtr0088553  NM_057845   1594   300 wun-RB FBtr0088552  NM_165674   2007   379
Additional Transcript Data & Comments
Reported size (kB)	1.622 (longest cDNA) (Zhang et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein wun-PA   FBpp0087636   33.9   300   9.11     NP_477193   AAM71066 wun-PB   FBpp0087635   42.8   379   8.45     NP_724787   AAF58942
Additional Polypeptide Data & Comments
Reported size (kD)	300 (aa) (Zhang et al., 1997)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0016078
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Phosphatidic acid phosphatase type 2/haloperoxidase (IPR000326) Phosphatidic acid phosphatase/chloroperoxidase, N-terminal (IPR016118)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA940783   AC008341   AE003833 AAF58942   AE003833 AAM71066   AF145595 AAD38570   AI062852   AI401851   AI514677   AI518107   AI534417   AQ025894   AQ026117   AQ073398   AQ073721   AY046533 AAL34392   BI164505   BI216140   BI241795   BI370211   BI374044   BI486317   BI486929   BI580438   BP557646   BT001729 AAN71484   CK659648   CK659964   CO191706   CO191753   CO276346   CO320936   CO335632   CW848181   CZ471901   CZ473592   CZ473687   EC051723   EC052206   EC054646   EC054650   EC055944   EC066749   EC204786   EC208943   EC265914   U73822 AAC47449   Z71084
UniProtKB/Swiss-Prot	Q9V576
UniProtKB/TrEMBL
Maps to	FBcl0297657 (FlyBase, 1992-)
Does NOT map to
Identified with	GH02203 (Hayden, 1999.3.3, Rubin, 1999.4.23, BDGP Project Members, 2000-) RE70417 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13216
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | late  embryonic central nervous system (Zhang et al., 1997)  embryonic stage  ectoderm | restricted (Zhang et al., 1997)  embryonic stage  posterior midgut primordium | dorsal (Zhang et al., 1997)  embryonic stage  hindgut primordium (Zhang et al., 1997)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI062852
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016078 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0016078 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0016078 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for wun Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable wunEY11935 viable, with Scer\GAL4[-]: wunEP2607 lethal | recessive wunEMS23 wunEMS41 wunEMS4 wunk09507 wunk10201 wunk15909 wunk16806 Other Phenotypes Allele wild-type wun+tNN neuroanatomy defective wunk09507 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 wunEP2208 Sterility Allele female sterile wunCE wunCN fertile wunEY11935 Phenotype manifest in Allele germ cell wunCE wunCN anterior fascicle, with Scer\GAL4elav-C155 wunEP2208 anterior fascicle & synapse, with Scer\GAL4elav-C155 wunEP2208 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 wunEP2208 germ cell, with Scer\GAL4[-]: wunEP2607 germ cell | female, with Scer\GAL4twi.PG wunScer\UAS.cZa germ cell | male, with Scer\GAL4twi.PG wunScer\UAS.cZa germ cell, with Scer\GAL4how-24B, Scer\GAL4twi.PG wunScer\UAS.cZa synapse wunk09507 bouton wunk09507 germ cell | female wunk10201 germ cell | male wunk10201 embryonic proventriculus (with Df(2R)wun-GL) wunk15909 embryonic digestive system (with Df(2R)wun-GL) wunk15909 germ cell, with Scer\GAL4twi.PG wunScer\UAS.T:Avic\GFP-EGFP
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of wun Class Mutagen Stocks Known lesion wunCE loss of function 0 -- wunCN 0 Yes wunEMS23 ethyl methanesulfonate 1 -- wunEMS41 ethyl methanesulfonate 1 -- wunEMS4 ethyl methanesulfonate 1 -- wunEMS9 ethyl methanesulfonate 0 -- wunEP2208 P-element activity 1 Yes wunEP2607 P-element activity 1 Yes wunEP2650ex34 Δ2-3 0 Yes wunEP652 P-element activity 1 -- wunEY11935 P-element activity 1 -- wunGS10009 0 Yes wund00931 P-element activity 0 -- wund03854 P-element activity 0 -- wund04014 P-element activity 0 -- wunk09507 P-element activity 1 Yes wunk10201 loss of function P-element activity 2 Yes wunk12382 0 -- wunk15909 P-element activity 1 -- wunk16806 P-element activity 2 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wun Class Mutagen Stocks Known lesion wun+tNN in vitro construct | genomic fragment 0 Yes wunD248T.Scer\UAS.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | amino acid replacement 0 Yes wunGD15706 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes wunGD1640 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes wunNX in vitro construct | genomic fragment 0 Yes wunScer\UAS.T:Avic\GFP-EGFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes wunScer\UAS.cZa in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)wun-GL   In(2LR)w45-32n   In(2R)w45-67n
Disrupted in	Df(2R)11 (Kozopas, 2003.3) Df(2R)Np5 (Spradling et al., 1999, Kozopas, 2003.3, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)w45-30n (BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)w73-1 (Zhang et al., 1996) Df(2R)wun-GL (Matthews, 1996.2.2, Zhang et al., 1996) In(2LR)TE35B-15LL2R (Zhang et al., 1996) In(2R)G63Lw45-73nR (Zhang et al., 1996)
Not disrupted in	Df(2R)H3C1 (Zhang et al., 1996) Df(2R)H3D3 (Zhang et al., 1996) Df(2R)H3E1 (Zhang et al., 1996) Df(2R)NCX10 (Zhang et al., 1996) Df(2R)NCX11 (Zhang et al., 1996) Df(2R)Np1 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)Np3 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)Np4 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)P14TE (Zhang et al., 1996) Df(2R)XE-916 (BDGP Project Members, 1994-1999) Df(2R)w45-30n (BDGP Project Members, 1994-1999) Df(2R)w73-1 (BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)wun-GL (Zhang et al., 1996) In(2LR)TE35B-15 (Zhang et al., 1996) In(2R)G63Lw45-73nR (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD15706} NA P{GD1640} NA P{UAS-wun.D248T.GFP} NA P{UAS-wun.EGFP} NA P{UAS-wun.Z} NA characterization construct P{NN} NA P{NX} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}l(2)0349703497 Yes P{lacW}l(2)SH0273SH0273 No P{lacW}wunk09507 No P{lacW}wunk10201 No P{lacW}wunk15909 No P{lacW}wunk16806 No insertion of mobile activating element P{EPgy2}wunEY11935 NA P{EP}EP2525 NA P{EP}wunEP2208 NA P{EP}wunEP2607 NA P{EP}wunEP652 NA P{GSV6}wunGS10009 NA P{XP}wund00931 NA P{XP}wund03854 NA P{XP}wund04014 NA
Related Comments
	Please look at the allele reports for the complete phenotype data The wun gene is involved in guiding germ cell migration. (Matthews, 1996.2.2) wun is required for guidance of germ cell migration in early development. Loss of wun function disrupts the orientation of motion causing germ cells to disperse even though their normal target is well formed. (Zhang et al., 1996) wun has two properties that allow it to use repulsion to guide the germ cells. wun can transform a permissive cellular environment into a repulsive one, and is expressed in the gut in a pattern that guides germ cells towards the mesoderm. Its sequence suggests that it is involved in lipid metabolism. (Zhang et al., 1997)
Gene Ontology: Function, Process & Cellular Component ( 12 )
Molecular Function
CV term Evidence References
phosphatidate phosphatase activity non-traceable author statement (Morrison et al., 2000) inferred from sequence or structural similarity (Hayden and Howard, 1999) non-traceable author statement (Swiss-Prot Project Members, 2002.6.15)
phosphoric monoester hydrolase activity inferred from direct assay (Burnett and Howard, 2003)
protein homodimerization activity traceable author statement (Pyne et al., 2004)
Biological Process
CV term Evidence References
dephosphorylation non-traceable author statement (Morrison et al., 2000) non-traceable author statement (Swiss-Prot Project Members, 2002.6.15)
embryonic pattern specification traceable author statement (Pyne et al., 2004)
germ cell migration non-traceable author statement (Zhang et al., 1997) traceable author statement (Wylie, 1999) non-traceable author statement (Russell, 2001.7.18) traceable author statement (Mueller, 2002)
germ cell programmed cell death traceable author statement (Coffman, 2003)
germ cell repulsion traceable author statement (Coffman, 2003) traceable author statement (Ribeiro et al., 2003) traceable author statement (Luquain et al., 2003) traceable author statement (Raz, 2004)
pole cell migration traceable author statement (Howard, 1998)
Cellular Component
CV term Evidence References
integral to membrane non-traceable author statement (Swiss-Prot Project Members, 2002.6.15)
membrane inferred from electronic annotation with InterPro:IPR000326 (FlyBase Curators et al., 2004-)
plasma membrane inferred from sequence or structural similarity (Zhang et al., 1997)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene