A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\dpa

General Information
SymbolDmel\dpaSpeciesD. melanogaster
Namedisc proliferation abnormalAnnotation symbolCG1616
Feature typeprotein_coding_geneFlyBase IDFBgn0015929
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map
Cytogenetic map43C6-43C7Sequence location2R:3,384,206..3,387,379 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene disc proliferation abnormal is referred to in FlyBase by the symbol dpa (CG1616, FBgn0015929). It has the cytological map location 43C6-43C7. Its sequence location is 2R:3384206..3387379. Its molecular function is described as: DNA binding; 3'-5' DNA helicase activity; ATP binding; nucleoside-triphosphatase activity. It is involved in the biological processes: DNA replication; mitotic spindle organization and biogenesis; DNA endoreduplication; DNA replication initiation. 11 alleles are reported. The phenotypes of these alleles are annotated with: mitotic cell cycle; mitotic metaphase; spindle; chromosome, pericentric region; imaginal disc; central nervous system. It has one annotated transcript and one annotated polypeptide.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
43C6-43C7  
Limits computationally determined from genome sequence between P{lacW}cosk16101 and P{EP}CG1600EP398  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
43C-43C
(determined by in situ hybridisation)  
(Feger et al., 1995)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: dpa- didum+
(Mermall et al., 2005)
hide Gene Model & Products
Please see the GBrowse view of Dmel\dpa for information on other features
detailed view FBtr0088983 FBtr0088982 FBtr0089744 FBtr0089743 FBtr0089745 FBpp0088056 FBpp0088055 FBpp0088685 FBpp0088686 FBpp0088684 FBti0029484 FBti0027038 FBti0036498 FBti0026203 FBti0072215 FBti0033263 FBti0023426
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
dpa-RA
FBtr0088982
 NM_057837
  2801
  866
Additional Transcript Data & Comments
Reported size (kB)
2.8 (northern blot)
(Feger et al., 1995)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
dpa-PA  
FBpp0088055  
96.6  
866  
7.57  
  NP_477185
  AAF59242
Additional Polypeptide Data & Comments
Reported size (kD)
866 (aa); 97 (kD predicted)
(Feger et al., 1995)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
MCM (IPR001208)
C-type lectin (IPR001304)
AAA+ ATPase, core (IPR003593)
MCM protein 4 (IPR008047)
Nucleic acid-binding, OB-fold (IPR012340)
Nucleic acid-binding, OB-fold-like (IPR016027)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA438548
 
AA539502
 
AC004280
 
AE003841
AAF59242
 
BI164385
 
BI231586
 
BP548801
 
BP548802
 
BQ102711
 
BT021329
AAX33477
 
CO176997
 
CO188874
 
CO265153
 
CO277701
 
CO280124
 
CO288601
 
CO288744
 
CO291528
 
CO306032
 
CO312411
 
CO314560
 
CO319086
 
CO323413
 
CO334073
 
EC057204
 
EC063129
 
EC063483
 
EC072768
 
EC081591
 
EC195382
 
EC195705
 
EC199289
 
EC202924
 
EC207624
 
EC210048
 
EC226219
 
EC233191
 
EC239946
 
S80255
AAB35644
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Maps to
(FlyBase, 1992-)
Does NOT map to
Identified with
(BDGP Project Members, 2000-)
hide Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
Type
Symbol & Location
Additional Notes
References
aberration junction
T(2;3)H36.bk1
2R:3,384,206..3,387,339
comment=aberration breakpoint reported to map within the dpa transcription unit
evidence=experimental
(Feger et al., 1995)
rescue fragment
dpa[+t12]
2R:3,380,249..3,392,319
comment=position of restriction fragment on reference sequence inferred by FlyBase curator
evidence=experimental
linked_to=EcoRI-EcoRI_rfrag
(Feger et al., 1995)
hide External Data
Linkouts
DEDB - Drosophila exon database: splicing graphs
•
Crossreferences
hide Expression Data
FlyBase-Curated Data
Transcript and
Protein data 		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
hide Summary of Transcript Expression
Stage
Tissue/Position
Reference
 embryonic stage | stage >=13
 embryonic brain
(Feger et al., 1995)
 embryonic stage | stage >=13
 embryonic/larval hindgut
(Feger et al., 1995)
 larval stage | third instar
 imaginal disc
(Feger et al., 1995)
 larval stage | third instar
 eye-antennal disc
(Feger et al., 1995)
 embryonic stage | stage >=12
 anterior embryonic/larval midgut
(Feger et al., 1995)
 embryonic stage | stage >=12
 Malpighian tubule
(Feger et al., 1995)
 embryonic stage | stage 10-12
 embryonic central nervous system | presumptive
(Feger et al., 1995)
 embryonic stage | stage <=11
 epidermis
(Feger et al., 1995)
 embryonic stage | stage 16,17
 gonad
(Feger et al., 1995)
 larval stage | third instar
 neuroblast
(Feger et al., 1995)
 oogenesis stage,adult stage
 oocyte
(Feger et al., 1995)
 embryonic stage | stage 10-12
 peripheral nervous system
(Feger et al., 1995)
 larval stage | third instar
 optic lobe
(Feger et al., 1995)
 oogenesis stage,adult stage
 germ cell
(Feger et al., 1995)
 embryonic stage | stage >=12
 posterior embryonic/larval midgut
(Feger et al., 1995)
 oogenesis stage,adult stage
 nurse cell
(Feger et al., 1995)
Marker for
    Subcellular Localization
    CV Term
    hide Summary of Polypeptide Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide External Data & Images
      Linkouts
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      •
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      •
      GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
      •
      BDGP expression data
      •
      Show all BDGP original images, annotations and embryonic microarray data for dpa
      FlyExpress - Embryonic expression images (BDGP data)
      •
      Show all FlyExpress processed images for dpa
      hide Alleles & Phenotypes
      hide Summary of Allele Phenotypes
      Lethality
      Allele
      lethal | pupal stage | recessive
      Other Phenotypes
      Allele
      wild-type
      Phenotype manifest in
      Allele
      mitotic cell cycle
      mitotic metaphase
      spindle
      mitosis & nuclear chromosome
      chromosome, pericentric region
      imaginal disc
      central nervous system
      hide Classical Alleles ( 9 )
      For All Classical Alleles Show

      Allele of dpaClassMutagenStocksKnown lesion
      dpa1amorph2 --
      dpa20 --
      dpa311.50 --
      dpaDG297121 --
      dpaEY040151 --
      dpaH36
        		0 Yes
        dpad280 Yes
        dpak100090 Yes
        dpaunspecified
          		0 --
          hide Alleles Carried on Transgenic Constructs ( 2 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of dpaClassMutagenStocksKnown lesion
          dpa+t120 Yes
          dpaGD44451 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
          Disrupted in
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Ryder, 2004.4.26)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Feger et al., 1995)
          (Heitzler et al., 1993)
          (Mermall et al., 2005)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Feger et al., 1995)
          Not disrupted in
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Roote and Ashburner, 2005.6)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          (Heitzler et al., 1993)
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          UAS construct
          P{GD4445}
          NA
          characterization construct
          P{dpa+12}
          NA
          Insertions
          Type of insertions
          Name
          Expression data
          miscellaneous insertions
          P{lacW}dpak10009
          NA
          P{wHy}dpaDG29712
          NA
          insertion of enhancer trap
          P{MTW}dpa311.5
          No
          P{lacW}l(2)SH0520SH0520
          No
          insertion of mobile activating element
          P{EPgy2}dpaEY04015
          NA
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          dpa is required for the regulation of DNA replication in neuroblasts and for DNA replication in mitotically proliferating cells.
          (Feger et al., 1995)
          hide Gene Ontology: Function, Process & Cellular Component ( 9 )
          hide Molecular Function
          CV term
          Evidence
          References
          contributes_to
          3'-5' DNA helicase activity
          inferred from direct assay
          (Moyer et al., 2006)
          ATP binding
          inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008047
          (FlyBase Curators et al., 2004-)
          DNA binding
          non-traceable author statement
          (Swiss-Prot Project Members, 1997.2.1)
          inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008047
          (FlyBase Curators et al., 2004-)
          nucleoside-triphosphatase activity
          inferred from electronic annotation with InterPro:IPR003593
          (FlyBase Curators et al., 2004-)
          hide Biological Process
          CV term
          Evidence
          References
          NOT
          DNA endoreduplication
          non-traceable author statement
          (Swiss-Prot Project Members, 1997.2.1)
          DNA replication
          inferred from sequence or structural similarity with UniProtKB:P30664 AND inferred from sequence or structural similarity with UniProt:P33991
          (FlyBase, 1992-)
          inferred from mutant phenotype
          (Pflumm and Botchan, 2001)
          traceable author statement
          (Lee and Orr-Weaver, 2003)
          non-traceable author statement
          (Swiss-Prot Project Members, 1997.2.1)
          DNA replication initiation
          inferred from electronic annotation with InterPro:IPR008047
          (FlyBase Curators et al., 2004-)
          mitotic spindle organization and biogenesis
          inferred from mutant phenotype
          (Pflumm and Botchan, 2001)
          hide Cellular Component
          CV term
          Evidence
          References
          nucleus
          non-traceable author statement
          (Swiss-Prot Project Members, 1997.2.1)
          inferred from electronic annotation with InterPro:IPR008047
          (FlyBase Curators et al., 2004-)
          hide Sequence Ontology: Class of Gene
          protein_coding_gene
           
          nuclear_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          dpa allele
          Gene
          References
          dpa311.5
          ph-p
          (Faucheux et al., 2001)
          hide External Data
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
           
          Linkouts
          InParanoid orthologs - Eukaryotic orthologs
          •
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 6 )
          VDRC
          v44484
          w1118; P{GD4445}v44484
          Szeged
          -
          P{lacW}l(2)SH0520SH0520
          Kyoto
          107594
          Dp(?;2)bwD, dpa1 bwD/CyO
          Bloomington
          4126
          Dp(?;2)bwD, dpa1 bwD/CyO
          20530
          y1 w67c23; P{wHy}dpaDG29712/SM6a
          15922
          y1 w67c23; P{EPgy2}dpaEY04015/CyO
          hide Genomic Clones ( 1 )
          hide cDNA Clones ( 19 )
          Please Note
          This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          hide Antibody Information
          polyclonal
          (Su et al., 1997)
          hide Other Information
          hide Discoverer
          hide Etymology
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for identity of: dpa CG1616
          (Gabrielian, 1999.11)
          Source for database merge of
          Additional comments
          hide Comments About Role
          hide Comments About Molecular Function
          The Sld