Gene Dmel\dah

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General Information

Symbol

Dmel\dah

Species

D. melanogaster

Name

discontinuous actin hexagon

Annotation symbol

CG6157

Feature type

protein_coding_gene

FlyBase ID

FBgn0015926

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

13B6-13B6

Sequence location

X:15,222,379..15,225,035 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene discontinuous actin hexagon is referred to in FlyBase by the symbol dah (CG6157, FBgn0015926). It has the cytological map location 13B6. Its sequence location is X:15222379..15225035. Its molecular function is described as zinc ion binding. It is involved in the biological processes: cellularization; cytokinesis, contractile ring formation. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13B6-13B6

Limits computationally determined from genome sequence between P{EP}hiw^EP1308 and P{EP}Ahcy13^EP1007

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

13C1-13C1

(Brown, 1996.11.26)

Experimentally Determined Recombination Data

Location

(Brown, 1996.11.26)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: Top1? dah?

(Zhang et al., 1996)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Top1+ dah+

(Brown et al., 1998, Brown, 1996.11.26)

Gene Model & Products

Please see the GBrowse view of Dmel\dah for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

dah-RA

FBtr0074007

NM_078607

2349

649

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

dah-PA

FBpp0073824

72.1

649

7.65

NP_511162

AAF48441

Additional Polypeptide Data & Comments

Reported size (kD)

649 (aa)

(Brown et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015926

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, ZZ-type (IPR000433)

EF-hand, region 1 (IPR015153)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

dah[+tDSR]
X:15,221,482..15,226,837

comment=The boundaries of the rescue fragment appear to correspond to a BamHI-EcoRI fragment on the map in Figure 1; position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=SalI-EcoRI_rfrag

(Zhang et al., 1996)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2885

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI515538

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015926

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015926

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015926

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

dah^f04432

lethal | embryonic stage | maternal effect

Other Phenotypes

Allele

wild-type

dah^+tDSR

Sterility

Allele

fertile

dah^f04432

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of dah	Mutagen	Stocks	Known lesion
dah³⁰	Δ2-3	1	Yes
dah³⁶	Δ2-3	0	Yes
dah^f04432	piggyBac transposase	2	--
dah^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of dah	Mutagen	Stocks	Known lesion
dah^+tDSR	in vitro construct \| genomic fragment	0	Yes
dah^GD16555	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
dah^dsRNA.cRa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

T(1;3)143-3

Disrupted in

Df(1)dah³⁰

(Zhang et al., 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD16555}

characterization construct

P{DSR}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}dah^f04432

Related Comments

Please look at the allele reports for the complete phenotype data

Mutants arrest in embryonic development during the transition from syncytial to cellular blastoderm. Expression patterns and sequence homologies suggest dah may play an essential role in organising and maintaining a specialised cytoskeletal structure.

(Zhang et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

zinc ion binding

inferred from electronic annotation with InterPro:IPR000433

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

cellularization

traceable author statement

(Tepass et al., 2001)

traceable author statement

(Mazumdar and Mazumdar, 2002)

cytokinesis, contractile ring formation

non-traceable author statement

(Tepass et al., 2001)

Cellular Component

CV term

Evidence

References

extrinsic to membrane

traceable author statement

(Mazumdar and Mazumdar, 2002)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

dah allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

58814

Orthologs

Genome-wide drosophilid orthologs

Dana\GF21234

Dere\GG17875

Dgri\GH24488

Dmoj\GI10895

Dper\GL14572

Dpse\GA19399

Dsec\GM19870

Dsim\GD17206

Dvir\GJ16570

Dwil\GK20020

Dyak\GE17179

Curated drosophilid orthologs

Dpse\GA19399

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000018547
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Harvard

PBac{WH}dah^f04432

Bloomington

5812

w^* dah³⁰/FM6

18769

w¹¹¹⁸ PBac{WH}dah^f04432

VDRC

v50168

w¹¹¹⁸; P{GD16555}v50168

Genomic Clones ( 1 )

BACR48D17

cDNA Clones ( 30 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones