Gene Dmel\crq

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General Information

Symbol

Dmel\crq

Species

D. melanogaster

Name

croquemort

Annotation symbol

CG4280

Feature type

protein_coding_gene

FlyBase ID

FBgn0015924

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

21C2-21C2

Sequence location

2L:448,254..453,023 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene croquemort is referred to in FlyBase by the symbol crq (CG4280, FBgn0015924). It has the cytological map location 21C2. Its sequence location is 2L:448254..453023. Its molecular function is described as scavenger receptor activity. It is involved in the biological processes described with 11 unique terms, many of which group under: transport; programmed cell death; endocytosis; cell death; vesicle-mediated transport; immune response; membrane organization and biogenesis; defense response; cell adhesion; regulation of cellular component organization and biogenesis; cell activation; anatomical structure development. 14 alleles are reported. The phenotypes of these alleles are annotated with: embryonic central nervous system; embryonic glial cell. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21C2-21C2

Limits computationally determined from genome sequence between P{lacW}l(2)k09610^k09610&P{lacW}S^k09538a and P{PZ}l(2)10685¹⁰⁶⁸⁵&P{lacW}Tango14^k00619

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

21C3-21C4

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: ex? crq? ush?

(Franc et al., 1999)

Gene Model & Products

Please see the GBrowse view of Dmel\crq for information on other features

Comments on Gene Model

The carboxy terminus of the CDS does not agree with swiss-prot protein Q27367, but is well supported by available evidence.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

crq-RA

FBtr0078087

NM_175943

2774

491

crq-RB

FBtr0078088

NM_175944

2524

491

Additional Transcript Data & Comments

Reported size (kB)

2.3 (compiled cDNA)

(Franc et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

crq-PA

FBpp0077747

56.2

491

4.67

NP_787957

AAF51494

crq-PB

FBpp0077748

56.2

491

4.67

NP_787958

AAN10497

Additional Polypeptide Data & Comments

Reported size (kD)

68 (kD observed); 54 (kD predicted)

(Franc et al., 1996)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015924

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

CD36 antigen (IPR002159)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q27367

UniProtKB/TrEMBL

Maps to

DS00764

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

Does NOT map to

Identified with

RE02070

(BDGP Project Members, 2000-)

D1375

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5807

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

larval stage

embryonic/larval hemocyte

(Franc et al., 1996)

embryonic stage | stage >=11 | late

hemocyte

(Franc et al., 1996)

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI064105

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015924

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015924

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015924

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for crq

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for crq

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

crq^EY14489

lethal | pupal stage

crq^KG01679

Other Phenotypes

Allele

neuroanatomy defective

Sterility

Allele

fertile

crq^EY14489

Phenotype manifest in

Allele

embryonic central nervous system

embryonic glial cell

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of crq	Mutagen	Stocks	Known lesion
crq^EY14489	P-element activity	1	--
crq^KG01679	P-element activity	1	Yes
crq^c01854	piggyBac transposase	0	--
crq^c01936	piggyBac transposase	1	--
crq^c04725	piggyBac transposase	0	--
crq^f00184	piggyBac transposase	1	--
crq^f02039	piggyBac transposase	0	--
crq^f05578	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of crq	Mutagen	Stocks	Known lesion
crq^GD784	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
crq^Scer\UAS.cFa	in vitro construct \| regulatory fusion	0	Yes
crq^{dsRNA.1209-1737}	in vitro construct \| RNAi	0	Yes
crq^{dsRNA.681-1203}	in vitro construct \| RNAi	0	Yes
crq^dsRNA.cMa	in vitro construct \| RNAi	0	Yes
crq^dsRNA.cSa	in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(2L)al

Useful duplication

Dp(2;2)M24F⁺14

Disrupted in

Df(2L)TE99(Z)XW88

(Franc et al., 1999)

Df(2L)al

(Franc et al., 1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD784}

P{UAS-crq.F}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}crq^KG01679

P{UASp-YFP.Rab23}CG4164⁰²

insertion of mobile activating element

P{EPgy2}crq^EY14489

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 14 )

Molecular Function

CV term

Evidence

References

scavenger receptor activity

inferred from sequence or structural similarity with FLYBASE:emp; FB:FBgn0010435

(Franc et al., 1996)

non-traceable author statement

(Swiss-Prot Project Members, 1999.4.15)

traceable author statement

(Franc and White, 2000)

non-traceable author statement

(Lemaitre, 2000.12.20)

traceable author statement

(Theopold et al., 2002)

traceable author statement

(Evans and Banerjee, 2003)

Biological Process

CV term

Evidence

References

apoptosis

inferred from mutant phenotype

(Franc et al., 1999)

non-traceable author statement

(Swiss-Prot Project Members, 1999.4.15)

traceable author statement

(Kimbrell and Beutler, 2001)

traceable author statement

(Theopold et al., 2002)

traceable author statement

(Franc, 2002)

autophagic cell death

traceable author statement

(Thummel, 2001)

inferred from expression pattern

(Gorski et al., 2003)

cell adhesion

inferred from electronic annotation with InterPro:IPR002159

(FlyBase Curators et al., 2004-)

defense response

non-traceable author statement

(Lemaitre, 2000.12.20)

immune response

traceable author statement

(Schneider, 2000)

traceable author statement

(Kimbrell and Beutler, 2001)

macrophage activation

traceable author statement

(Evans and Banerjee, 2003)

phagocytosis

inferred from mutant phenotype

(Franc et al., 1999)

traceable author statement

(Meister, 2004)

traceable author statement

(Brennan and Anderson, 2004)

phagocytosis triggered by activation of immune response cell surface activating receptor

inferred from direct assay

(Stuart et al., 2005)

phagocytosis, engulfment

traceable author statement

(Franc and White, 2000)

traceable author statement

(Thummel, 2001)

traceable author statement

(Franc, 2002)

regulation of phagocytosis

traceable author statement

(Evans and Banerjee, 2003)

salivary gland cell autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

Cellular Component

CV term

Evidence

References

integral to plasma membrane

non-traceable author statement

(Swiss-Prot Project Members, 1999.4.15)

non-traceable author statement

(Lemaitre, 2000.12.20)

membrane

inferred from electronic annotation with InterPro:IPR002159

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

crq allele

Gene

References

unspecified

Adgf-A

(Dolezal et al., 2005)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59475

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24773

Dere\GG24648

Dgri\GH10516

Dmoj\GI18033

Dper\GL19261

Dpse\GA18078

Dsec\GM16668

Dsim\crq

Dvir\GJ19730

Dwil\GK14659

Dyak\crq

Curated drosophilid orthologs

Dpse\GA18078

Dsim\crq

Dyak\crq

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012288
Anopheles gambiae (Ensembl)
ENSXETP00000002583
Xenopus tropicalis (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

v45883

w¹¹¹⁸; P{GD784}v45883

v45884

w¹¹¹⁸; P{GD784}v45884/TM3

Bloomington

20939

y¹ w^67c23; P{EPgy2}crq^EY14489

14900

y¹ w^67c23; P{SUPor-P}crq^KG01679

Genomic Clones ( 1 )

BACR16A12

cDNA Clones ( 40 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones