Gene Dmel\caup

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Can be 1.5% recombination between ara and mirr, with none between ara and caup.

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: caup+ mirr?

(Kehl et al., 1998, Netter et al., 1998)

In direction of increasing cytology: ara+ caup+ mirr+

In direction of increasing cytology: ara? caup? anon-69Da? anon-69Db? mirr+

(Diez del Corral et al., 1999)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\caup for information on other features

Comments on Gene Model

DGC clone appears problematic (LP03275): incomplete CDS.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

caup-RA

FBtr0075909

NM_079322

3832

693

Additional Transcript Data & Comments

Reported size (kB)

4.7, 3.4, 1.3 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

caup-PA

FBpp0075641

73.7

693

6.99

NP_524046

AAF49895

Additional Polypeptide Data & Comments

Reported size (kD)

693 (aa)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015919

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Homeobox (IPR001356)

Iroquois-class homeodomain protein (IPR003893)

Homeodomain-like (IPR009057)

Homeodomain-related (IPR012287)

TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles

•

T01994

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P54269

UniProtKB/TrEMBL

Maps to

DS08512

(BDGP Project Members, 2000-)

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

4265

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage | third instar

wing vein L5

larval stage | third instar

alula

adult stage

embryonic stage | late

embryonic/larval proventriculus

embryonic stage | late

procephalic segment

larval stage

larval ventral ganglion

larval stage | third instar

tegula

larval stage | third instar

wing vein L1

larval stage | third instar

wing vein L3

larval stage | third instar

mesothoracic pleurum

larval stage

imaginal disc

embryonic stage-adult stage

larval stage | second instar

dorsal metathoracic disc

larval stage | third instar

dorsal metathoracic disc

embryonic stage-adult stage

embryonic stage | stage >=11

epidermis | lateral

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI259784

External Data & Images

Show all BDGP original images, annotations and embryonic microarray data for caup

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0015919

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0015919

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015919

BDGP expression data

•

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for caup

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

caup¹

lethal | recessive

Other Phenotypes

Allele

visible

visible, with Scer\GAL4^ey.PH

caup^{Scer\UAS.cdCa}

tissue polarity defective, with Scer\GAL4^Act5C.PI

caup^{Scer\UAS.cdCa}

Sterility

Allele

female sterile | recessive

caup¹

male sterile | recessive

caup¹

Phenotype manifest in

Allele

macrochaeta

microchaeta

caup¹

mesothoracic segment

caup¹

notopleural suture

caup¹

scutellum

caup¹

wing

caup¹

macrochaeta & scutum

caup¹

eye, with Scer\GAL4^ey.PH

caup^{Scer\UAS.cdCa}

vertical bristle, with Scer\GAL4^ey.PH

caup^{Scer\UAS.cdCa}

scutum & microchaeta | medial, with Scer\GAL4^sca-537.4

caup^{Scer\UAS.cdCa}

eye | ectopic, with Scer\GAL4^Act5C.PI

caup^{Scer\UAS.cdCa}

eye, with Scer\GAL4^Act5C.PI

caup^{Scer\UAS.cdCa}

ommatidium, with Scer\GAL4^Act5C.PI

caup^{Scer\UAS.cdCa}

dorsal margin photoreceptor | ectopic, with Scer\GAL4^GMR.PF

caup^{Scer\UAS.cdCa}

Classical Alleles ( 9 )

For All Classical Alleles Show

Allele of caup	Class	Mutagen	Stocks	Known lesion
caup¹	amorph, loss of function	X ray	0	Yes
caup^BG01626		P-element activity	1	Yes
caup^DFM1		Δ2-3	0	--
caup^DFM2	amorph, hypomorph	P-element activity Δ2-3	0	Yes
caup^L8		diepoxybutane	0	--
caup^L		P-element activity	0	--
caup^MB00278			1	--
caup^Sc2		P-element activity	0	Yes
caup^rF209	loss of function	P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of caup	Mutagen	Stocks	Known lesion
caup^GD1399	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
caup^{Scer\UAS.cdCa}	in vitro construct \| regulatory fusion	0	Yes
caup^dsRNA.cLa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)iro-2

Useful duplication

Dp(3;3)M67C⁺5

Disrupted in

Df(3L)iro-2

(Kehl et al., 1998, Gomez-Skarmeta et al., 1996)

Df(3L)iro-DFM1

Df(3L)iro-DFM3

In(3L)iro-DFM2

Tp(3;3)iro-1

(Dambly-Chaudiere and Leyns, 1992)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1399}

P{UAS-caup.dC}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{GT1}caup^BG01626

insertion of enhancer trap binary system

Mi{ET1}caup^MB00278

insertion of enhancer trap

P{MTW}caup^L

P{PZ}ara^rF209

Yes

P{lacW}caup^Sc2

Related Comments

Please look at the allele reports for the complete phenotype data

Gene titration method has been used to search for genes involved in the determination of sense organs. A mutation of ara and caup (formerly iro) demonstrates an interaction with Df(4)M101-62f, a chromosome known to alter the development of the PNS.

In mutants for the Iroquois gene complex, two lateral bands of the notum are completely devoid of sense organs.

(Leyns et al., 1991)

ara-caup expression at patches on the wing, located one at each side of the DV compartment border, is mediated by the hh signal through its induction of high levels of ci protein in anterior cells near to the AP compartment border. High levels of ci activate dpp expression and together ci and dpp positively control ara-caup expression. The posterior border of the patches is defined by repression by en. wg accumulation at the DV border sets, also by repression, the gap between the two patches.

(Gomez-Skarmeta and Modolell, 1996)

When the Iroquois complex is absent, notum cells are transformed into hinge (tegula and sclerite) cells.

(Diez del Corral et al., 1999)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR001356

specific RNA polymerase II transcription factor activity

non-traceable author statement

(Modolell, 1996.1.16)

transcription factor activity

inferred from electronic annotation with InterPro:IPR001356

transcription regulator activity

non-traceable author statement

(Swiss-Prot Project Members, 1996.10.1)

Biological Process

CV term

Evidence

References

compound eye morphogenesis

inferred from mutant phenotype

(Tomlinson, 2003)

equator specification

traceable author statement

(McNeill, 2000)

phagocytosis, engulfment

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

regulation of transcription from RNA polymerase II promoter

non-traceable author statement

(Swiss-Prot Project Members, 1996.10.1)

regulation of transcription, DNA-dependent

inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR003893

(Swiss-Prot Project Members, 1996.10.1)

Cellular Component

CV term

Evidence

References

nucleus

non-traceable author statement

(Modolell, 1996.1.16)

non-traceable author statement

inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR003893

(Dominguez and de Celis, 1998)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

caup allele

Gene

References

caup^DFM1

ara

(Calleja et al., 2000)

caup^{Scer\UAS.cdCa}

unspecified

dally

(Fujise et al., 2001)

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64789

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24689

Dere\GG15585

Dgri\GH14716

Dmoj\GI12262

Dper\GL25204

Dpse\GA10434

Dsec\GM25358

Dsim\GD14390

Dvir\GJ11493

Dwil\GK10562

Dyak\GE21913

Curated drosophilid orthologs

Dpse\GA10434

Drep\caup

y¹ w^67c23; Mi{ET1}caup^MB00278

InParanoid orthologs - Eukaryotic orthologs

•

ENSCAFP00000015338
Canis familiaris (Ensembl)
ENSCINP00000020014
Ciona intestinalis (Ensembl)
NEWSINFRUP00000154687
Takifugu rubripes (Ensembl)
ENSGALP00000021522
Gallus gallus (Ensembl)
ENSP00000231357
Homo sapiens (Ensembl)
ENSP00000290552
Homo sapiens (Ensembl)
ENSMMUP00000005641
Macaca mulatta (Ensembl)
ENSMMUP00000015303
Macaca mulatta (Ensembl)
GSTENP00013874001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000019512
Xenopus tropicalis (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v2931

w¹¹¹⁸; P{GD1399}v2931

v2932

w¹¹¹⁸; P{GD1399}v2932/CyO

Bloomington

12788

w¹¹¹⁸; P{GT1}caup^BG01626

22754

Genomic Clones ( 1 )

BACR01O14

cDNA Clones ( 13 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP04760

Other clones

RNAi & Array Information

Affy Oligo

(Dambly-Chaudiere and Leyns, 1992)

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0015919

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0015919

Antibody Information

Other Information

Discoverer

Etymology

Etymology: "Iroquois" is the name given to the gene complex by analogy with the American tribe that have a haircut ascribed to the Mohawk nation.

Etymology: the "caup" gene is named after a hero of the Araucanian American-Indian tribe.

Etymology: the 'Iroquois' gene complex is named after the native American Iroquois tribe, also called Mohawks, which shaved all but a medial stripe of hairs on the head.

(Leyns et al., 1996)

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: caup CG10605

(Bayraktaroglu, 1999.11)

Source for database merge of

Additional comments

Comments About Role

The caup gene maps to the Iroquois complex and contributes to the prepattern that regulates expression of ac-sc complex genes and rho. caup is a positive, direct regulator of the ac-sc genes.

The genes of the IRO-C are coordinately regulated and implicated in similar developmental processes. They are regulated in the eye by the Pc-group and trx-group genes, but not by classical modifiers of position effect variegation. Ventral silencing of the whole IRO-C in the eye occurs at the level of chromatin structure in a manner similar to that of the homeotic gene complexes, perhaps by local compaction of the region into a heterochromatin-like structure involving Pc-group products.

(Diez del Corral et al., 1999)

The genes of the Iroquois complex (ara, caup and mirr) are necessary for notum specification. The Iro-C genes establish a signalling system that appears to organize development in the notum and dorsal wing hinge (tegula and sclerite) territories.

The localised expression of the three genes of the iroquois complex - IRO-C (ara, caup and mirr) specifies the identity of dorsal cells in the eye. Juxtaposition of IRO-C-expressing and non-expressing cells forms a straight border that promotes growth and serves as a pattern-organising centre in the eye disc.

(Cavodeassi et al., 1999)

Iroquois complex genes (ara, caup and mirr) are required for the specification of dorsal head structures.

(Cavodeassi et al., 2000)

Comments About Molecular Function

Other Comments

A member of the IRO-C (Iroquois complex).