A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\α-Adaptin

General Information
SymbolDmel\α-AdaptinSpeciesD. melanogaster
Nameα-AdaptinAnnotation symbolCG4260
Feature typeprotein_coding_geneFlyBase IDFBgn0015567
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map21C2-21C2Sequence location2L:408,022..414,775 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
This gene is referred to in FlyBase by the symbol α-Adaptin (CG4260, FBgn0015567). It has the cytological map location 21C2. Its sequence location is 2L:408022..414775. Its molecular function is described as: protein transporter activity; protein binding. It is involved in the biological processes: synaptic vesicle transport; asymmetric cell division; neurotransmitter secretion; synaptic vesicle coating; vesicle coating; vesicle-mediated transport; protein localization; intracellular protein transport. 10 alleles are reported. The phenotypes of these alleles are annotated with: synapse; wing; wing vein; anterior fascicle; macrochaeta; socket; external sensory organ precursor cell IIb; external sensory organ precursor cell IIa; microchaeta. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
21C2-21C2  
Limits computationally determined from genome sequence between P{lacW}RpI135k16513&P{lacW}ebik16213 and P{lacW}l(2)k09610k09610&P{lacW}Sk09538a  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
21C-21C
Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.  
(Lloyd et al., 2000)
21C1-21C2
 
(Gonzalez Gaitan, 1997.2.5)
21C2-21C3
 
(Dornan, 1997.5.9)
21C1-21C2
(determined by in situ hybridisation)  
(Spradling et al., 1999)
21C2-21C3
(determined by in situ hybridisation)  
(Dornan et al., 1997)
21C1-21C2
(determined by in situ hybridisation)  
(Forjanic et al., 1997)
21C1-21C2
(determined by in situ hybridisation)  
(Gonzalez-Gaitan and Jackle, 1997)
21C1-21C2
 
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\α-Adaptin for information on other features
detailed view FBtr0078054 FBtr0089489 FBtr0089488 FBtr0078055 FBpp0077714 FBpp0088945 FBpp0088490 FBpp0077715 FBti0004442 FBti0007726 FBti0039723 FBti0026231 FBti0010425 FBti0006364
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
alpha-Adaptin-RB
FBtr0089489
 NM_205885
  4286
  952
α-Adaptin-RA
FBtr0089488
 NM_057471
  4717
  940
Additional Transcript Data & Comments
Reported size (kB)
6.3, 4.8 (northern blot)
(Gonzalez-Gaitan and Jackle, 1997)
5.1, 4.3 (northern blot)
(Dornan et al., 1997)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
alpha-Adaptin-PB  
FBpp0088945  
106.9  
952  
6.52  
  NP_995607
  AAS64634
α-Adaptin-PA  
FBpp0088490  
105.6  
940  
7.20  
  NP_476819
  AAF56103
Additional Polypeptide Data & Comments
Reported size (kD)
939 (aa); 105 (kD predicted)
(Dornan et al., 1997)
940 (aa)
(Gonzalez-Gaitan and Jackle, 1997)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Clathrin/coatomer adaptor, adaptin-like, N-terminal (IPR002553)
Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain (IPR003164)
Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain (IPR008152)
Clathrin/coatomer adaptor, adaptin-like, appendage, C-terminal subdomain (IPR009028)
Armadillo-like helical (IPR011989)
Clathrin adaptor, alpha-adaptin, appendage, Ig-like subdomain (IPR013038)
Clathrin/coatomer adaptor, adaptin-like, appendage, Ig-like subdomain (IPR013041)
Clathrin alpha-adaptin/coatomer adaptor, appendage, C-terminal subdomain (IPR015873)
Armadillo-type fold (IPR016024)
Adaptor protein complex AP-2, alpha subunit (IPR017104)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA141001
 
AA816246
 
AC004275
 
AC005762
 
AE003589
AAF56103
 
AE003589
AAS64634
 
AI109855
 
AI258931
 
AI532543
 
AI534180
 
AI534550
 
AI543697
 
AQ024987
 
AQ073654
 
BF495956
 
BF502777
 
BG634370
 
BG636915
 
BG640270
 
BI215476
 
BI229666
 
BI237349
 
BI356252
 
BI356329
 
BI357894
 
BI358546
 
BI484465
 
BI486874
 
BI564339
 
BI578453
 
BI583744
 
BI585182
 
BI586849
 
BI587972
 
BI588108
 
BI588778
 
BI589245
 
BI605496
 
BI615108
 
BI626901
 
BI630138
 
BI633776
 
BI640038
 
BT003458
AAO39461
 
BT032839
 
CO177003
 
CO190340
 
CO261816
 
CO263089
 
CO268019
 
CO269901
 
CO270740
 
CO271726
 
CO278743
 
CO279944
 
CO280468
 
CO286978
 
CO295460
 
CO299348
 
CO307697
 
CO309993
 
CO312934
 
CO316956
 
CO317477
 
CO318377
 
CO319331
 
CO342121
 
EC056681
 
EC057356
 
EC058216
 
EC059538
 
EC060124
 
EC065090
 
EC066894
 
EC071358
 
EC072478
 
EC081061
 
EC084790
 
EC087461
 
EC088976
 
EC206953
 
EC208163
 
EC208764
 
EC213004
 
EC215371
 
EC219572
 
EC227008
 
EC228652
 
EC243161
 
EC243678
 
EC245128
 
EC250107
 
EC254787
 
G00611
 
Y11104
CAA71991
 
Y13092
CAA73533
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (Spradling et al., 1999)
    (BDGP Project Members, 2000-, Stapleton et al., 2006)
    (BDGP Project Members, 1994-1999, Spradling et al., 1999)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage
     embryonic brain
    (Gonzalez-Gaitan and Jackle, 1997)
     embryonic stage,adult stage
     
    (Gonzalez-Gaitan and Jackle, 1997)
     embryonic stage,adult stage
     
    (Gonzalez-Gaitan and Jackle, 1997)
     larval stage
     imaginal disc
    (Gonzalez-Gaitan and Jackle, 1997)
     embryonic stage
     embryonic ventral nervous system
    (Gonzalez-Gaitan and Jackle, 1997)
     embryonic stage
     garland cell
    (Gonzalez-Gaitan and Jackle, 1997)
     embryonic stage,larval stage
     
    (Dornan et al., 1997)
     embryonic stage,larval stage
     
    (Dornan et al., 1997)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage
       proctodeum | primordium
      (Dornan et al., 1997)
       embryonic stage | stage 8-12
       posterior midgut primordium
      (Dornan et al., 1997)
       pupal stage
       sensory organ precursor cell
      (Berdnik et al., 2002)
       embryonic stage | stage >=8
       embryonic nervous system
      (Dornan et al., 1997)
       pupal stage
       sensory organ precursor cell
      (Berdnik et al., 2002)
       embryonic stage
       ventral nerve cord
      (Dornan et al., 1997)
       embryonic stage | early
       
      (Dornan et al., 1997)
       larval stage
       bouton
      (Dornan et al., 1997)
       embryonic stage | stage >=14
       endoderm
      (Dornan et al., 1997)
       embryonic stage
       embryonic stomatogastric nervous system
      (Dornan et al., 1997)
       embryonic stage | stage >=14
       antenno-maxillary complex
      (Dornan et al., 1997)
       larval stage
       neuromuscular junction
      (Dornan et al., 1997)
       embryonic stage
       fat body primordium
      (Dornan et al., 1997)
       embryonic stage | stage >=13
       embryonic peripheral nervous system
      (Dornan et al., 1997)
       embryonic stage | stage >=14
       garland cell
      (Dornan et al., 1997)
       embryonic stage | stage >=14
       visceral mesoderm
      (Dornan et al., 1997)
       embryonic stage
       chordotonal organ
      (Dornan et al., 1997)
       embryonic stage | late
       larval somatic muscle
      (Dornan et al., 1997)
       embryonic stage
       
      (Dornan et al., 1997)
       embryonic stage | stage 8-11
       neuroblast
      (Dornan et al., 1997)
       embryonic stage
       procephalic mesoderm
      (Dornan et al., 1997)
       embryonic stage
       cephalic furrow
      (Dornan et al., 1997)
       embryonic stage
       supraoesophageal ganglion
      (Dornan et al., 1997)
       embryonic stage
       adult midgut precursor
      (Dornan et al., 1997)
       embryonic stage | stage 8-12
       anterior midgut primordium
      (Dornan et al., 1997)
      Marker for
        Subcellular Localization
        CV Term
        plasma membrane
        (Gonzalez-Gaitan and Jackle, 1997)
        cytoplasm plasma membrane
        (Dornan et al., 1997)
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0078054 FBtr0089489 FBtr0089488 FBtr0078055
        AI532543
        AA941227
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        lethal | somatic clone
        lethal | larval stage | recessive
        lethal | pupal stage | recessive
        lethal, with Scer\GAL4elav.PLu
        Other Phenotypes
        Allele
        visible | conditional - heat sensitive (with α-Adaptin3)
        visible | conditional - heat sensitive (with α-Adaptin4)
        locomotor behavior defective
        locomotor behavior defective | conditional - heat sensitive
        uncoordinated | conditional - heat sensitive
        flightless
        neurophysiology defective
        neuroanatomy defective
        Phenotype manifest in
        Allele
        synapse
        wing | conditional - heat sensitive (with α-Adaptin4)
        wing vein | conditional - heat sensitive (with α-Adaptin4)
        wing | conditional - heat sensitive (with α-Adaptin3)
        wing vein | conditional - heat sensitive (with α-Adaptin3)
        anterior fascicle, with Scer\GAL4elav-C155
        anterior fascicle & synapse, with Scer\GAL4elav-C155
        macrochaeta | somatic clone
        socket | ectopic | somatic clone
        external sensory organ precursor cell IIb | somatic clone
        external sensory organ precursor cell IIa | ectopic | somatic clone
        microchaeta | somatic clone
        hide Classical Alleles ( 9 )
        For All Classical Alleles Show

        Allele of α-AdaptinClassMutagenStocksKnown lesion
        α-Adaptin06694hypomorph1 Yes
        α-Adaptin20 Yes
        α-Adaptin3amorph0 Yes
        α-Adaptin4hypomorph0 --
        α-AdaptinEP25191 --
        α-AdaptinEP8961 Yes
        α-Adaptinear26hypomorph0 Yes
        α-Adaptinear40 Yes
        α-Adaptinear50 Yes
        hide Alleles Carried on Transgenic Constructs ( 1 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of α-AdaptinClassMutagenStocksKnown lesion
        α-AdaptinGD50592 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Disrupted in
        (Spradling et al., 1999, Berdnik et al., 2002, BDGP Project Members, 1994-1999, Gonzalez-Gaitan and Jackle, 1997)
        (Ryder, 2004.4.26)
        Not disrupted in
        (BDGP Project Members, 1994-1999)
        (Spradling et al., 1999)
        (BDGP Project Members, 1994-1999)
        (Spradling et al., 1999, BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        P{PZ-Δ.a2}α-Adaptin2
        NA
        insertion of enhancer trap
        P{PZ}retn05096
        Yes
        P{PZ}α-Adaptin06694
        Yes
        P{lacW}l(2)SH0460SH0460
        No
        insertion of mobile activating element
        P{EP}α-AdaptinEP2519
        NA
        P{EP}α-AdaptinEP896
        NA
        P{Mae-UAS.6.11}B0756B0756
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        In presynaptic terminals α-Adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited. α-Adaptin is necessary for the formation of clathrin coated pits and participates in the dynamin-dependent release of coated vesicles from the membrane surface. Resu