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Gene Dmel\unpg

General Information
Symbol	Dmel\unpg	Species	D. melanogaster
Name	unplugged	Annotation symbol	CG1650
Feature type	protein_coding_gene	FlyBase ID	FBgn0015561
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	45B3-45B3	Sequence location	2R:5,082,719..5,087,377 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene unplugged is referred to in FlyBase by the symbol unpg (CG1650, FBgn0015561). It has the cytological map location 45B3. Its sequence location is 2R:5082719..5087377. Its molecular function is described as: transcription factor activity; RNA polymerase II transcription factor activity; sequence-specific DNA binding. It is involved in the biological processes: regulation of transcription; regulation of nervous system development; brain development; open tracheal system development; tracheal outgrowth, open tracheal system; regulation of transcription, DNA-dependent. 8 alleles are reported. The phenotypes of these alleles are annotated with 13 unique terms, many of which group under: embryonic/larval tracheal system; organ system; tracheal system; trachea; cerebral anastomosis; anatomical structure; adult segment; adult mesothoracic segment; ganglionic branch 2; thoracic segment. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45B3-45B3   Limits computationally determined from genome sequence between P{lacW}CG8788k04512&P{lacW}l(2)k13412k13412 and P{PZ}Pkn06736
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45C-45C   (Chiang, 1995.9.6) 44B-44B Mapping method not described.   (Cui and Doe, 1995) 45C-45C (determined by in situ hybridisation)   (Chiang et al., 1995) 45C-45C (determined by in situ hybridisation)   (Doe, 1992)
Experimentally Determined Recombination Data
Location	2- (Chiang, 1995.9.6)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\unpg for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) unpg-RA FBtr0088586  NM_057798   1683   485
Additional Transcript Data & Comments
Reported size (kB)	1.8 (northern blot) (Chiang et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein unpg-PA   FBpp0087667   52.2   485   7.32     NP_477146   AAF58968
Additional Polypeptide Data & Comments
Reported size (kD)	485 (aa) (Chiang et al., 1995)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0015561
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox (IPR001356) Homeodomain-like (IPR009057) Homeodomain-related (IPR012287)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC008186   AE003834 AAF58968   BI574402   BI574619   BI575219   BI577038   BI583642   BI585605   BI589103   BI589138   BI620911   BT022753   EL874486   U35427 AAA97404
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q4V5A3
Maps to	FBcl0297656 (FlyBase, 1992-)
Does NOT map to
Identified with	RH23414 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References regulatory region unpg-reg_element-1 2R:5,086,752..5,089,270 comment=Drives unpg-like expression of a reporter construct in the CNS and the tracheal system; position of restriction fragment on reference sequence inferred by FlyBase curator evidence=experimental linked_to=XhoI-EcoRI_rfrag (Chiang et al., 1995)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13238
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 13  metathoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 11  mesothoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 8  ventral midline (Chiang et al., 1995)  embryonic stage | stage 8  neuroblast (Chiang et al., 1995)  embryonic stage | stage 9  ectoderm | lateral <of> prothoracic segment (Chiang et al., 1995)  embryonic stage | stage 14  embryonic/larval ganglionic branch (Chiang et al., 1995)  embryonic stage | stage 13  abdominal tracheal pit 1..7 (Chiang et al., 1995)  embryonic stage  central nervous system (Chiang et al., 1995)  embryonic stage | stage 13  mesothoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 9  ectoderm | lateral <of> labial segment (Chiang et al., 1995)  embryonic stage | stage 13  cerebral branch (Chiang et al., 1995)  embryonic stage  tracheal system (Chiang et al., 1995)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage 13  mesothoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 13  cerebral branch | precursor (Chiang et al., 1995)  embryonic stage | stage 11  mesothoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 9  ectoderm | lateral <of> prothoracic segment (Chiang et al., 1995)  embryonic stage | stage 14  embryonic/larval ganglionic branch (Chiang et al., 1995)  embryonic stage | stage 13  abdominal tracheal pit 1..7 (Chiang et al., 1995)  embryonic stage | stage 8  ventral midline (Chiang et al., 1995)  embryonic stage | stage 13  metathoracic tracheal pit (Chiang et al., 1995)  embryonic stage | stage 8  neuroblast (Chiang et al., 1995)  embryonic stage  central nervous system (Chiang et al., 1995)  embryonic stage | stage 9  ectoderm | lateral <of> labial segment (Chiang et al., 1995)  embryonic stage  tracheal system (Chiang et al., 1995)
Marker for
Subcellular Localization
CV Term	nucleus (Chiang et al., 1995)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015561 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0015561 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0015561 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for unpg Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive unpgr1 unpgr221 unpgr225 unpgr37 Phenotype manifest in Allele cerebral branch unpgr1 unpgr225 unpgr37 cerebral anastomosis unpgr1 unpgr225 unpgr37 ganglionic branch 0 unpgr1 unpgr225 unpgr37 ganglionic branch 1 unpgr1 unpgr225 unpgr37 ganglionic branch 2 unpgr1 unpgr225 unpgr37 ganglionic branch 3 unpgr1 unpgr225 unpgr37 ganglionic branch 4 unpgr1 unpgr225 unpgr37 ganglionic branch 5 unpgr1 unpgr225 unpgr37 ganglionic branch 6 unpgr1 unpgr225 unpgr37 ganglionic branch 7 unpgr1 unpgr225 unpgr37 ganglionic branch 8 unpgr1 unpgr225 unpgr37 ganglionic branch 9 unpgr1 unpgr225 unpgr37 wing unpgr37
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of unpg Class Mutagen Stocks Known lesion unpg1912 wild-type allele P-element activity 0 Yes unpgE22 wild-type allele P-element activity 0 Yes unpgf85 wild-type allele P-element activity 0 Yes unpgr1 loss of function Δ2-3 0 Yes unpgr221 loss of function Δ2-3 0 Yes unpgr225 loss of function Δ2-3 0 Yes unpgr37 loss of function Δ2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of unpg Class Mutagen Stocks Known lesion unpgGD4069 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)w45-30n
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{U1} No P{U2} No P{U3} No P{U4} No P{U5} No P{U6} No UAS construct P{GD4069} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{3'lacZ}unpgr1 NA P{3'lacZ}unpgr37 NA P{5'lacZ}unpgr221 NA P{5'lacZ}unpgr225 NA insertion of enhancer trap P{lacZ-un4}unpg1912 Yes P{lacZ}unpgE22 No P{lacZ}unpgf85 Yes
Related Comments
	Please look at the allele reports for the complete phenotype data unpg is a regulatory target for genes of the bithorax complex and is required for formation of the tracheal branches that penetrate the CNS. (Chiang et al., 1995) cas, eve, unpg and ac are expressed in specific neuroblast sublineages. Expression studies using pbl and stg mutants suggest that neuroblasts have an intrinsic gene regulatory hierarchy controlling unpg and ac expression but that cell cycle- or cytokinesis-dependent mechanisms are required for cas and eve CNS expression. (Cui and Doe, 1995)
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
RNA polymerase II transcription factor activity inferred from sequence or structural similarity with EMBL:AB075028 (FlyBase, 1992-)
sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
transcription factor activity inferred from mutant phenotype (Chiang et al., 1995)
Biological Process
CV term Evidence References
brain development inferred from mutant phenotype (Hirth et al., 2003)
open tracheal system development traceable author statement (Uv et al., 2003)
regulation of nervous system development inferred from mutant phenotype (Chiang et al., 1995)
regulation of transcription inferred from mutant phenotype (Chiang et al., 1995)
regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR001356 (FlyBase Curators et al., 2004-)
tracheal outgrowth, open tracheal system traceable author statement (Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Chiang et al., 1995) inferred by curator from GO:0003702 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions unpg allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61780
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11812 Dere\GG23425 Dgri\GH20573 Dmoj\GI19766 Dper\GL16839 Dpse\GA14073 Dsec\GM21108 Dsim\GD10643 Dvir\GJ18251 Dwil\GK17836 Dyak\GE19264
Curated drosophilid orthologs	Dpse\GA14073
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000021664 Anopheles gambiae (Ensembl) ENSBTAP00000011716 Bos taurus (Ensembl) ENSCAFP00000017900 Canis familiaris (Ensembl) NEWSINFRUP00000130948 Takifugu rubripes (Ensembl) ENSGALP00000021739 Gallus gallus (Ensembl) ENSP00000302251 Homo sapiens (Ensembl) ENSMMUP00000007770 Macaca mulatta (Ensembl) ENSMODP00000009342 Monodelphis domestica (Ensembl) ENSPTRP00000022357 Pan troglodytes (Ensembl) ENSRNOP00000026419 Rattus norvegicus (Ensembl) ENSXETP00000007133 Xenopus tropicalis (Ensembl) ENSXETP00000007145 Xenopus tropicalis (Ensembl) Q6Q7E0 Oryza sativa (Gramene) Q8H7N5 Oryza sativa (Gramene) ZDB-CDNA-040425-333 Danio rerio (Zfin) ZDB-GENE-020509-2 Danio rerio (Zfin) MGI:95668 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v12567 w1118; P{GD4069}v12567 v12568 w1118; P{GD4069}v12568/TM3
Genomic Clones ( 1 )
	BACR10M14
cDNA Clones ( 10 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP14723
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH30882 RH22304 RH25916 RH25223 RH52170 RH30782 RH29728 RH33902
Other clones	IP14523
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0015561 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0015561
Antibody Information
	polyclonal (Chiang et al., 1995)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: unpg CG1650 (Heiman, 1999.11)
Source for database merge of
Additional comments
Comments About Role
	Expression analysed in CNS study of neuroblasts and ganglion mother cells, using an enhancer trap to reveal the expression pattern. (Doe, 1992)
Comments About Molecular Function
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057798 RefSeq (Proteins) • NP_477146 DDBJ / EMBL / GenBank • AC008186 AE003834 AAF58968 BI574402 U35427 AAA97404 UniProtKB/TrEMBL • Q4V5A3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Homeobox (IPR001356) Homeodomain-like (IPR009057) Homeodomain-related (IPR012287)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61780 DEDB - Drosophila exon database: splicing graphs • 13238 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0015561 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015561 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0015561 FlyExpress - Embryonic expression images (BDGP data) • FBgn0015561 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015561 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0015561 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0015561 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000021664 Anopheles gambiae (Ensembl) ENSBTAP00000011716 Bos taurus (Ensembl) ENSCAFP00000017900 Canis familiaris (Ensembl) NEWSINFRUP00000130948 Takifugu rubripes (Ensembl) ENSGALP00000021739 Gallus gallus (Ensembl) ENSP00000302251 Homo sapiens (Ensembl) ENSMMUP00000007770 Macaca mulatta (Ensembl) ENSMODP00000009342 Monodelphis domestica (Ensembl) ENSPTRP00000022357 Pan troglodytes (Ensembl) ENSRNOP00000026419 Rattus norvegicus (Ensembl) ENSXETP00000007133 Xenopus tropicalis (Ensembl) ENSXETP00000007145 Xenopus tropicalis (Ensembl) Q6Q7E0 Oryza sativa (Gramene) Q8H7N5 Oryza sativa (Gramene) ZDB-CDNA-040425-333 Danio rerio (Zfin) ZDB-GENE-020509-2 Danio rerio (Zfin) MGI:95668 Mus musculus (Informatics) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/unplug1.htm PANTHER - Protein classification by function, families, and pathways • FBgn0015561 REDfly - Regulatory element database for Drosophila • FBgn0015561
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	anon-45C   CG1650   Gbx (Hirth et al., 2005) unp (Franch-Marro and Casanova, 2002, Chiang, 1995.9.6, Cui and Doe, 1995, Chiang et al., 1995, ) unpg   upg (Schmid and Doe, 1998, Broadus et al., 1995)
Name Synonym	unplugged
Secondary FlyBase IDs
	FBgn0014101
References ( 30 )
Generate a list of	All references relating to this gene ( 30 )
List References by type	Research paper  ( 9 ) Review  ( 7 ) Personal communication to FlyBase  ( 2 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Conference report  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2006 Show research papers ...