Gene Dmel\tap

(BDGP Project Members, 1994-1999)

74B1-74B2

(determined by in situ hybridisation)

(Perrimon et al., 1996)

73D1-73D2

(determined by in situ hybridisation) 74B1--2 (determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\tap for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

tap-RA

FBtr0075216

NM_079400

2172

398

Additional Transcript Data & Comments

Reported size (kB)

2.106 (compiled cDNA)

1.392 (longest cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

tap-PA

FBpp0074978

44.9

398

7.01

NP_524124

AAF49352

Additional Polypeptide Data & Comments

Reported size (kD)

407 (aa)

398 (aa)

Comments

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015550

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Basic helix-loop-helix dimerisation region bHLH (IPR001092)

Helix-loop-helix DNA-binding (IPR011598)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O16867

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE52048

Dm0632

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

3759

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 10,11

neuroblast NB4-1

embryonic stage | stage 10-15

ventral nerve cord | restricted

embryonic stage

embryonic peripheral nervous system | restricted

embryonic stage

embryonic nervous system | restricted

embryonic stage | stage 11

embryonic ganglion mother cell

embryonic stage | stage 11

neuroblast

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage

v\\\'es2 neuron

Marker for

Subcellular Localization

CV Term

nucleus

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI404308

External Data & Images

Show all BDGP original images, annotations and embryonic microarray data for tap

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015550

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015550

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015550

BDGP expression data

•

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for tap

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Other Phenotypes

Allele

tarsal response defective | conditional - heat sensitive

tap^hs.PL

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of tap	Class	Mutagen	Stocks	Known lesion
tap⁰¹⁶⁵⁸		P-element activity	1	--
tap^j2B12		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of tap	Mutagen	Stocks	Known lesion
tap^GD16795	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
tap^GD4129	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
tap^hs.PL	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)81k19

Df(3L)st7

Useful duplication

Dp(3;3)st⁺g18

Disrupted in

Df(3L)81k19

(Ledent et al., 1998, BDGP Project Members, 1994-1999)

Df(3L)Exel6131

(Ryder, 2004.4.26)

Df(3L)tap-74

(Johnson et al., 1999)

Not disrupted in

Df(3L)st-j7

(BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{bps-lacZ}

Yes

UAS construct

P{GD16795}

P{GD4129}

heat-shock construct

P{hs-tap.L}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}tap^j2B12

Related Comments

Please look at the allele reports for the complete phenotype data

The expression of tap, which encodes a basic HLH protein, is dependent on Poxn.

(Ledent et al., 1995)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. tap gene expression during oogenesis is not critical to embryonic development, but the gene function may be essential for fertilisation and/or completion of meiosis.

(Perrimon et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

specific RNA polymerase II transcription factor activity

non-traceable author statement

(Swiss-Prot Project Members, 1999.12.15)

transcription regulator activity

inferred from electronic annotation with InterPro:IPR001092, InterPro:IPR011598

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

regulation of transcription

inferred from electronic annotation with InterPro:IPR001092, InterPro:IPR011598

(FlyBase Curators et al., 2004-)

regulation of transcription from RNA polymerase II promoter

non-traceable author statement

(Swiss-Prot Project Members, 1999.12.15)

sensory organ development

traceable author statement

(Skaer et al., 2002)

Cellular Component

CV term

Evidence

References

nucleus

non-traceable author statement

(Swiss-Prot Project Members, 1999.12.15)

inferred from electronic annotation with InterPro:IPR001092, InterPro:IPR011598

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

tap allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65225

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522225

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10169

Dere\GG13612

Dgri\GH16587

Dmoj\GI12435

Dper\GL15578

Dpse\GA20508

Dsec\GM25695

Dsim\GD14703

Dvir\GJ12331

Dwil\GK16635

Dyak\GE19908

Curated drosophilid orthologs

Dpse\GA20508

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000018581
Anopheles gambiae (Ensembl)
ENSBTAP00000004322
Bos taurus (Ensembl)
ENSCAFP00000020911
Canis familiaris (Ensembl)
NEWSINFRUP00000152663
Takifugu rubripes (Ensembl)
ENSGALP00000014468
Gallus gallus (Ensembl)
ENSP00000317333
Homo sapiens (Ensembl)
ENSMMUP00000011740
Macaca mulatta (Ensembl)
ENSMODP00000018070
Monodelphis domestica (Ensembl)
ENSPTRP00000018017
Pan troglodytes (Ensembl)
GSTENP00012076001
Tetraodon nigroviridis (Ensembl)
WBGene00034567
Caenorhabditis briggsae (Wormbase)
ZDB-CDNA-040425-572
Danio rerio (Zfin)
ZDB-CDNA-040425-573
Danio rerio (Zfin)
ZDB-GENE-990415-172
Danio rerio (Zfin)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

VDRC

v12638

w¹¹¹⁸; P{GD4129}v12638

v12639

w¹¹¹⁸; P{GD4129}v12639

v46805

w¹¹¹⁸; P{GD16795}v46805

v46806

w¹¹¹⁸; P{GD16795}v46806

Bloomington

11542

tap⁰¹⁶⁵⁸ P{PZ}blot⁰¹⁶⁵⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Genomic Clones ( 1 )

BACR22B18

cDNA Clones ( 7 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE52048

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

EK160861

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0015550

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0015550

Antibody Information

monoclonal

Other Information

Discoverer

Etymology

Etymology: 'biparous' means producing two offspring, based on its expression in progenitor cells that give rise to two types of cells.

(Dambly-Chaudiere and Vervoort, 1998)

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: tap bps Source for merge of: tap l(3)01658

(Ledent et al., 1998)

Source for merge of: tap bps

Additional comments

Comments About Role

tap is expressed in both neuronal and glial precursors after they have progressed beyond the ectodermal vs neuronal decision but before they differentiate into mature neurons or glia. Unlike other neural bHLH genes, expression persists until very late stages of embryogenesis.

Comments About Molecular Function

Other Comments

tap is expressed in a small subset of neurons when they undergo differentiation. In the peripheral nervous system, tap is expressed exclusively in one of the neurons that innervates each larval chemosensory organ, possibly controlling the specific properties of that neuron. Expression of tap is regulated by Poxn.