A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\crl

General Information
SymbolDmel\crlSpeciesD. melanogaster
NamecourtlessAnnotation symbolCG4443
Feature typeprotein_coding_geneFlyBase IDFBgn0015374
Created / Updated2004-08-16/2004-08-16
Genomic Location
Chromosome (arm)XRecombination map2-
Cytogenetic map14F1-14F1Sequence locationX:16,487,712..16,489,453 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene courtless is referred to in FlyBase by the symbol crl (CG4443, FBgn0015374). It has the cytological map location 14F1. Its sequence location is X:16487712..16489453. Its molecular function is described as ubiquitin-protein ligase activity. It is involved in the biological processes: male courtship behavior; spermatogenesis; ubiquitin cycle; male meiosis; courtship behavior; regulation of protein metabolic process. 11 alleles are reported. The phenotypes of these alleles are annotated with: spermatid; meiotic cell cycle. It has one annotated transcript and one annotated polypeptide.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
14F1-14F1  
Limits computationally determined from genome sequence between P{EP}EP1547EP1547 and P{EP}CG4928EP1341  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
47D-47D
(determined by in situ hybridisation)  
(Orgad et al., 2000)
47D-47D
 
(Orgad et al., 1995)
Experimentally Determined Recombination Data
Location
2-
(Orgad et al., 1995)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\crl for information on other features
detailed view FBtr0074291 FBtr0074290 FBtr0074283 FBtr0074289 FBpp0074066 FBpp0074065 FBpp0074058 FBpp0074064 FBti0078196 FBti0039433 FBti0055247
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
crl-RA
FBtr0074283
 NM_079945
  1437
  167
Additional Transcript Data & Comments
Reported size (kB)
1.3 (northern blot)
(Orgad et al., 2000)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
crl-PA  
FBpp0074058  
18.5  
167  
4.42  
  NP_524684
  AAF48626
Additional Polypeptide Data & Comments
Reported size (kD)
200, 185 (aa); 22, 20 (kD predicted)
(Orgad et al., 2000)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Ubiquitin-conjugating enzyme, E2 (IPR000608)
Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA949415
 
AE003502
AAF48626
 
AI946405
 
AJ277746
CAB90824
 
AY071319
AAL48941
 
BF488153
 
BF496713
 
BG638522
 
BI170989
 
BI232958
 
BI235527
 
BI237833
 
BI238585
 
BI242700
 
BI354346
 
BI356544
 
BI358694
 
BI365003
 
BI485505
 
BI568046
 
BI575617
 
BI578728
 
BI583889
 
BI586065
 
BI586908
 
BI587095
 
BI587548
 
BI606919
 
BI614831
 
BI617198
 
BI620322
 
BI626429
 
CL528005
 
CO156277
 
CO262631
 
CO266243
 
CO274299
 
CO274649
 
CO276074
 
CO277168
 
CO277486
 
CO294333
 
CO315524
 
CO341909
 
CO343699
 
CZ473435
 
CZ473436
 
EC059977
 
EC066397
 
EC071951
 
EC072108
 
EC199037
 
EC206438
 
EC219307
 
EC222683
 
EC242380
 
EC243627
 
EC248260
 
EC250293
 
EC254997
 
EC261166
 
EC264803
 
LD28310
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Oliver, 2001.8.16)
    (BDGP Project Members, 2000-, Mukai et al., 2006)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage-adult stage
     
    (Orgad et al., 2000)
     adult stage | male
     
    (Orgad et al., 2000)
     embryonic stage | stage 10
     embryonic neuroblast
    (Orgad et al., 2000)
     embryonic stage | stage 15
     embryonic central nervous system
    (Orgad et al., 2000)
     embryonic stage | stage 6
     cephalic furrow
    (Orgad et al., 2000)
     embryonic stage | stage 4
     
    (Orgad et al., 2000)
     embryonic stage | stage 10
     stomodeal invagination
    (Orgad et al., 2000)
     embryonic stage | stage 6
     mesoderm
    (Orgad et al., 2000)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage | stage 7-8
       endoderm
      (Orgad et al., 2000)
       embryonic stage | stage 13-17
       embryonic central nervous system
      (Orgad et al., 2000)
       embryonic stage | blastoderm
       
      (Orgad et al., 2000)
       embryonic stage | stage 7-8
       mesoderm
      (Orgad et al., 2000)
      Marker for
      Subcellular Localization
      CV Term
      hide Microarray Data
      Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
      Untitled Document detailed view FBtr0074291 FBtr0074290 FBtr0074283 FBtr0074289
      AA949415
      hide External Data & Images
      Linkouts
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      •
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      •
      GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
      •
      hide Alleles & Phenotypes
      hide Summary of Allele Phenotypes
      Lethality
      Allele
      viable
      lethal | larval stage | recessive
      Other Phenotypes
      Allele
      wild-type
      courtship defective | recessive
      courtship defective | male | recessive
      meiotic cell cycle defective | male | recessive
      Sterility
      Allele
      male sterile | recessive
      fertile
      female fertile
      Phenotype manifest in
      Allele
      spermatid
      meiotic cell cycle | male
      hide Classical Alleles ( 9 )
      For All Classical Alleles Show

      Allele of crlClassMutagenStocksKnown lesion
      crl10 Yes
      crl5-4
        		0 Yes
        crl52-13
          		0 Yes
          crl59-7
            		0 Yes
            crl6-8
              		0 Yes
              crl60-7
                		0 Yes
                crlEY073401 --
                crlP40 Yes
                crld035721 --
                hide Alleles Carried on Transgenic Constructs ( 2 )
                For All Alleles Carried on Transgenic Constructs Show

                Allele of crlClassMutagenStocksKnown lesion
                crlGD105202 Yes
                crlt4.30 Yes
                hide Aneuploid Aberrations
                Useful deficiency
                 
                hide Transgenic Constructs & Insertions
                Transgenic Constructs
                Type of construct
                Name
                Expression data
                UAS construct
                P{GD10520}
                NA
                characterization construct
                P{crlt4.3}
                NA
                Insertions
                Type of insertions
                Name
                Expression data
                miscellaneous insertions
                P{Car20}crlP4
                NA
                P{}crl1
                NA
                insertion of mobile activating element
                P{EPgy2}crlEY07340
                NA
                P{XP}crld03572
                NA
                hide Related Comments
                Please look at the allele reports for the complete phenotype data
                hide Gene Ontology: Function, Process & Cellular Component ( 7 )
                hide Molecular Function
                CV term
                Evidence
                References
                ubiquitin-protein ligase activity
                inferred from sequence or structural similarity
                (Orgad et al., 1997)
                non-traceable author statement
                (Orgad, 2000.5.7)
                inferred from sequence or structural similarity with UniProtKB:O00102
                (FlyBase, 1992-)
                non-traceable author statement
                (Greenspan and Ferveur, 2000)
                hide Biological Process
                CV term
                Evidence
                References
                courtship behavior
                non-traceable author statement
                (Billeter et al., 2002)
                traceable author statement
                (Greenspan and Ferveur, 2000)
                non-traceable author statement
                (Sokolowski, 2001)
                male courtship behavior
                inferred from mutant phenotype
                (Orgad et al., 1997)
                inferred from mutant phenotype
                (Orgad et al., 2000)
                traceable author statement
                (Emmons and Lipton, 2003)
                male meiosis
                inferred from mutant phenotype
                (Orgad et al., 2000)
                regulation of protein metabolic process
                inferred from electronic annotation with InterPro:IPR000608
                (FlyBase Curators et al., 2004-)
                spermatogenesis
                inferred from mutant phenotype
                (Orgad et al., 1997)
                inferred from mutant phenotype
                (Orgad et al., 2000)
                traceable author statement
                (Greenspan and Ferveur, 2000)
                ubiquitin cycle
                inferred from sequence or structural similarity
                (Orgad et al., 1997)
                inferred from sequence or structural similarity with UniProtKB:O00102
                (FlyBase, 1992-)
                hide Cellular Component
                CV term
                Evidence
                References
                hide Sequence Ontology: Class of Gene
                protein_coding_gene
                 
                nuclear_gene
                 
                hide Interactions & Pathways
                hide Summary of Genetic Interactions
                Interacts with
                Please look at the allele data for full details of the genetic interactions
                crl allele
                Gene
                References
                hide External Data
                Linkouts
                BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
                •
                hide Orthologs
                Genome-wide drosophilid orthologs
                Curated drosophilid orthologs
                 
                 
                Linkouts
                InParanoid orthologs - Eukaryotic orthologs
                •
                hide Functional Complementation between Species
                hide Inter-Species Misexpression Data
                Produces phenotype in
                Produces NO phenotype in
                hide Stocks & Reagents
                hide Stocks Listed in FlyBase ( 4 )
                Harvard
                -
                P{XP}crld03572
                VDRC
                v34109
                w1118; P{GD10520}v34109
                v34111
                w1118; P{GD10520}v34111
                Bloomington
                16813
                y1 w67c23 P{EPgy2}crlEY07340
                hide Genomic Clones ( 1 )
                hide cDNA Clones ( 40 )
                Please Note
                This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
                cDNA Clones, Fully Sequenced
                BDGP DGC clones
                Other clones
                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                hide RNAi & Array Information
                Affy Oligo
                Linkouts
                DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
                •
                GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
                •
                hide Antibody Information
                polyclonal
                (Orgad et al., 2000)
                polyclonal antibody
                (Perrin et al., 2004)
                hide Other Information
                hide Discoverer
                hide Etymology
                hide Identification
                hide Position Effect Variegation Data
                hide Relationship to Other Genes
                Source for database identity of
                Source for identity of: Ubc7 CG4443 Source for identity of Ubc7 CG4443 was sequence comparison (date:000516).
                (FlyBase, 1996-)
                Source for database merge of
                Additional comments
                hide Comments About Role
                crl is involved in male courtship behaviour and spermatogenesis.
                (Orgad et al., 2000)
                hide Comments About Molecular Function
                hide Other Comments
                hide External Crossreferences & Linkouts
                Sequence Crossreferences
                RefSeq (Transcripts)
                •
                RefSeq (Proteins)
                •
                DDBJ / EMBL / GenBank
                •
                UniProtKB/TrEMBL
                •
                Other Crossreferences
                InterPro domains - A database of protein families, domains, and functional sites
                •
                Ubiquitin-conjugating enzyme, E2 (IPR000608)
                Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
                Linkouts
                BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
                •
                DEDB - Drosophila exon database: splicing graphs
                •
                DRSC