Gene Dmel\UbcD2

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General Information

Symbol

Dmel\UbcD2

Species

D. melanogaster

Name

Ubiquitin conjugating enzyme 2

Annotation symbol

CG6720

Feature type

protein_coding_gene

FlyBase ID

FBgn0015320

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

32A5-32A5

Sequence location

2L:10,765,562..10,768,005 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Ubiquitin conjugating enzyme 2 is referred to in FlyBase by the symbol UbcD2 (CG6720, FBgn0015320). It has the cytological map location 32A5. Its sequence location is 2L:10765562..10768005. Its molecular function is described as ubiquitin-protein ligase activity. It is involved in the biological processes: ubiquitin cycle; regulation of protein metabolic process. 7 alleles are reported. The phenotypes of these alleles are annotated with: neuroblast; scutellar bristle; tormogen cell; trichogen cell; macrochaeta. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32A5-32A5

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32A-32A

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

32A4-32A5

(determined by in situ hybridisation)

(Spradling et al., 1999)

32A-32B

(determined by in situ hybridisation)

(Matuschewski et al., 1996)

32A4-32A5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\UbcD2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

UbcD2-RA

FBtr0080115

NM_057789

1625

232

UbcD2-RB

FBtr0080116

NM_164943

1350

232

Additional Transcript Data & Comments

Reported size (kB)

1.5 (northern blot)

(Matuschewski et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

UbcD2-PA

FBpp0079704

24.4

232

8.47

NP_477137

AAF53008

UbcD2-PB

FBpp0079705

24.4

232

8.47

NP_723616

AAN10762

Additional Polypeptide Data & Comments

Reported size (kD)

232 (aa); 24.5 (kD predicted)

(Matuschewski et al., 1996)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015320

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-conjugating enzyme, E2 (IPR000608)

Ubiquitin-conjugating enzyme/RWD-like (IPR016135)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P52485

UniProtKB/TrEMBL

Q541C3

Maps to

FBcl0297517

(FlyBase, 1992-)

Does NOT map to

Identified with

(Oliver, 2001.8.16)

LD17255

(Spradling et al., 1999)

LD47532

(Abdelilah-Seyfried et al., 2000)

RE74673

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

7225

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015320

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015320

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015320

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for UbcD2

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

UbcD2^k13206

Other Phenotypes

Allele

visible, with Scer\GAL4^sca-537.4

UbcD2^EP2146b

neuroanatomy defective | somatic clone

UbcD2^k13206

Phenotype manifest in

Allele

scutellar bristle, with Scer\GAL4^sca-537.4

UbcD2^EP2146b

tormogen cell, with Scer\GAL4^sca-537.4

UbcD2^EP2146b

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

UbcD2^EP2146b

macrochaeta

UbcD2^L32BC

neuroblast | somatic clone

UbcD2^k13206

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of UbcD2	Mutagen	Stocks	Known lesion
UbcD2^EP2146b	P-element activity	0	Yes
UbcD2^EP2598	P-element activity	1	--
UbcD2^L32BC	P-element activity	0	Yes
UbcD2^c01581	piggyBac transposase	1	--
UbcD2^e01595	piggyBac transposase	1	--
UbcD2^k13206	P-element activity	2	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of UbcD2	Class	Mutagen	Stocks	Known lesion
UbcD2^GD14776		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful duplication

Dp(2;2)Mdh3

Disrupted in

Df(2L)BSC214

(Christensen and Cook, 2006.8.30)

Df(2L)BSC32

(Deal and Cook, 2003.7.11)

Df(2L)Exel8026

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14776}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}UbcD2^c01581

PBac{RB}UbcD2^e01595

insertion of enhancer trap

P{lArB}UbcD2^L32BC

P{lacW}UbcD2^k13206

insertion of mobile activating element

P{EP}UbcD2^EP2146b

P{EP}UbcD2^EP2598

Related Comments

Please look at the allele reports for the complete phenotype data

A UbcD2 cDNA has been cloned and sequenced.

(Matuschewski et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

ubiquitin-protein ligase activity

inferred from genetic interaction with SGD_LOCUS:UBC4; SGD:S0000286 AND inferred from genetic interaction with SGD_LOCUS:UBC5; SGD:S0002466 AND inferred from sequence or structural similarity with SGD_LOCUS:UBC4; SGD:S0000286 AND inferred from sequence or structural similarity with SGD_LOCUS:UBC5; SGD:S0002466

(Matuschewski et al., 1996)

inferred from sequence or structural similarity with NCBI_gi:4507779

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2001.8.20)

Biological Process

CV term

Evidence

References

regulation of protein metabolic process

inferred from electronic annotation with InterPro:IPR000608

(FlyBase Curators et al., 2004-)

ubiquitin cycle

inferred from sequence or structural similarity with SGD_LOCUS:UBC4; SGD:S0000286 AND inferred from sequence or structural similarity with SGD_LOCUS:UBC5; SGD:S0002466

(Matuschewski et al., 1996)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions