Gene Dmel\Ssl

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General Information

Symbol

Dmel\Ssl

Species

D. melanogaster

Name

Suppressor of Stellate-like

Annotation symbol

CG13591

Feature type

protein_coding_gene

FlyBase ID

FBgn0015300

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

60D1-60D1

Sequence location

2R:20,386,763..20,387,754 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Suppressor of Stellate-like is referred to in FlyBase by the symbol Ssl (CG13591, FBgn0015300). It has the cytological map location 60D1. Its sequence location is 2R:20386763..20387754. Its molecular function is described as: protein kinase CK2 activity; protein serine/threonine kinase activity; protein kinase activity; protein kinase CK2 regulator activity. It is involved in the biological process protein amino acid phosphorylation. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60D1-60D1

Limits computationally determined from genome sequence between P{PZ}bs⁰³²⁶⁷&P{lacW}l(2)k10502^k10502 and P{lacW}Nurf-38^k16102

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60B-60C

(Kalmykova, 1995)

60D1-60D2

(determined by in situ hybridisation)

(Kalmykova et al., 1997)

Experimentally Determined Recombination Data

Location

(Kalmykova, 1995)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Ssl for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Ssl-RA

FBtr0072351

NM_079124

872

219

Ssl-RB

FBtr0072352

NM_206216

786

219

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Ssl-PA

FBpp0072258

25.2

219

6.24

NP_523848

AAF47214

Ssl-PB

FBpp0072259

25.2

219

6.24

NP_995938

AAS64769

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015300

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Casein kinase II, regulatory subunit (IPR000704)

Casein kinase II, regulatory subunit, alpha-helical (IPR016149)

Casein kinase II, regulatory subunit, beta-sheet (IPR016150)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24536

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

AT25555

(BDGP Project Members, 2000-)

bs30a09

(Oliver, 2001.8.16)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

1705

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015300

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015300

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015300

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^αTub84B.PL

Ssl^Scer\UAS.cJa

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Ssl	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Ssl	Mutagen	Stocks	Known lesion
Ssl^{173.T:Ecol\lacZ}	in vitro construct \| coding region fusion	0	Yes
Ssl^{934.T:Ecol\lacZ}	in vitro construct \| coding region fusion	0	Yes
Ssl^GD8064	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Ssl^Scer\UAS.cJa	in vitro construct \| regulatory fusion	0	Yes
Ssl^a.βTub85D	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)Px2

Disrupted in

Df(2R)Exel6278

(Ryder, 2004.4.26)

Df(2R)Exel7185

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

UAS construct

characterization construct

P{βTub85D-Ssl.a}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

protein kinase CK2 activity

inferred from sequence or structural similarity

(Kalmykova et al., 1997)

inferred from sequence or structural similarity with SGD_LOCUS:CKB2; SGD:S0005565

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1998.4.15)

protein kinase CK2 regulator activity

inferred from direct assay

(Kalmykova et al., 2002)

protein kinase activity

non-traceable author statement

(Morrison et al., 2000)

protein serine/threonine kinase activity

non-traceable author statement

(Kalmykova, 1995)

Biological Process

CV term

Evidence

References

protein amino acid phosphorylation

inferred from sequence or structural similarity

(Kalmykova et al., 1997)

non-traceable author statement

(Morrison et al., 2000)

Cellular Component

CV term

Evidence

References

protein kinase CK2 complex

inferred from electronic annotation with InterPro:IPR000704, InterPro:IPR016149, InterPro:IPR016150

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Ssl allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63482

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11247

Dere\GG19923

Dgri\GH22749

Dper\GL20424

Dpse\GA25112

Dsec\GM11826

Dsim\GD24508

Dwil\GK19682

Dyak\GE11447

Curated drosophilid orthologs

Dpse\GA12385

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v17282

w¹¹¹⁸; P{GD8064}v17282

v17280

w¹¹¹⁸; P{GD8064}v17280/CyO

Genomic Clones ( 2 )

cDNA Clones ( 3 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT25555

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0015300

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0015300

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Ssl NEST:bs30a09

(Rana, 2004.7.8)

Additional comments

Comments About Role

Comments About Molecular Function

Ssl is in a regulated chromatin domain that includes testes-specific genes.

(Kalmykova et al., 2005)

Other Comments

Molecular characterisation of a euchromatic gene. Ssl is derived from CkIIβ gene by reverse transcriptase. This event resulted in the loss of the first three introns in the coding region of the ancestor gene. Since this event Ssl has evolved under selective pressure at the transcriptional level.

(Kalmykova et al., 1997)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q24536

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Casein kinase II, regulatory subunit (IPR000704)

Casein kinase II, regulatory subunit, alpha-helical (IPR016149)

Casein kinase II, regulatory subunit, beta-sheet (IPR016150)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63482

DEDB - Drosophila exon database: splicing graphs

•

1705

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0015300

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015300

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0015300

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0015300

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0015300

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0015300

PANTHER - Protein classification by function, families, and pathways

•

FBgn0015300

Synonyms & Secondary IDs ( 11 )

Reported As

Symbol Synonym

CG13591

(Morrison et al., 2000, )

CK2βtes

(Boutanaev et al., 2002, Kalmykova et al., 2002, Jauch et al., 2006, Kalmykova et al., 2005)

CKIIb1

(Morrison et al., 2000)

NEST:bs30a09

SSL

(Kalmykova, 1995, Kalmykova, 1995, Kalmykova et al., 1997, Kalmykova et al., 1997)

Ssl

(Mikhaylova et al., 2006, Kalmykova et al., 2005)

Su(Ste)l

βCK2tes

(Gvozdev et al., 2000, Polesskaya et al., 1999, Kalmykova et al., 1997, Gvozdev et al., 2005)

Name Synonym

regulatory subunit of protein kinase CK2

(Gvozdev et al., 2005)

Su(Ste)-like

(Kalmykova et al., 1997)

Suppressor of Stellate-like

Secondary FlyBase IDs

FBgn0014036
FBgn0045134

References ( 27 )

Generate a list of

All references relating to this gene ( 27 )

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Recent research papers ( 2 )

Jauch et al., 2006, Gene 374: 142--152: The Drosophila melanogaster DmCK2beta transcription unit encodes for functionally non-redundant protein isoforms. [FBrf0193130]
Mikhaylova et al., 2006, Proc. Natl. Acad. Sci. USA 103(32): 11975--11980: Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line. [FBrf0195382]

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Recent reviews (0)

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