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A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008

Gene Dmel\Pi3K92E

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General Information

Symbol

Dmel\Pi3K92E

Species

D. melanogaster

Name

Pi3K92E

Annotation symbol

CG4141

Feature type

protein_coding_gene

FlyBase ID

FBgn0015279

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

3R

Recombination map

Cytogenetic map

92F3-92F3

Sequence location

3R:16,454,694..16,460,430 [-]

Map ( GBrowse )

detailed view

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Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Pi3K92E (CG4141, FBgn0015279). It has the cytological map location 92F3. Its sequence location is 3R:16454694..16460430. Its molecular function is described by 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; binding. It is involved in the biological processes described with 22 unique terms, many of which group under: regulation of biological process; anatomical structure development; larval development; regulation of cell size; phosphorus metabolic process; instar larval development; positive regulation of biological process; cell growth; phospholipid metabolic process; transmembrane receptor protein tyrosine kinase signaling pathway; larval feeding behavior; gland morphogenesis; autophagy; second-messenger-mediated signaling; response to chemical stimulus. 19 alleles are reported. The phenotypes of these alleles are annotated with 26 unique terms, many of which group under: organ system; anatomical structure; peripheral nervous system; adult segment; nervous system; imaginal precursor; adult; adult mesothoracic segment; cytoplasmic part; thoracic segment. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

92F3-92F3

Limits computationally determined from genome sequence between P{PZ}l(3)10585¹⁰⁵⁸⁵ and P{EP}Synd^EP409

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

92E12-92E13

(Leevers, 1996.10.25)

92E12-92E13

(determined by in situ hybridisation)

(Leevers, 1996.12.5)

92E12-92E13

(determined by in situ hybridisation)

(Leevers et al., 1996)

92D-92D

(determined by in situ hybridisation)

(MacDougall et al., 1995)

92D1-92D4

(determined by in situ hybridisation)

(MacDougall and Leevers, 1995.10.6)

92E-92E

(Maier et al., 1992)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: anon-92Ea? anon-92Eb? anon-92Ec? H+ Pi3K92E?

(Maier et al., 1992)

In direction of increasing cytology: H+ Pi3K92E-

(Weinkove et al., 1999)

Gene Order (overall orientation not stated)

References

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Gene Model & Products

Please see the GBrowse view of Dmel\Pi3K92E for information on other features

detailed view

Comments on Gene Model

DGC2 data: check structure of 3' exon.

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Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

FBtr0083940

4396

1088

FBtr0083941

4323

1088

Additional Transcript Data & Comments

Reported size (kB)

3.712 (compiled cDNA)

(Leevers et al., 1996)

Comments

External Data

Crossreferences

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Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Pi3K92E-PA

FBpp0083348

127.0

1088

7.62

NP_650902

AAF55792

Pi3K92E-PB

FBpp0083349

127.0

1088

7.62

NP_732536

AAN14359

Additional Polypeptide Data & Comments

Reported size (kD)

1088 (aa); 127 (kD predicted)

(Leevers et al., 1996)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

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FBgn0015279

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Phosphoinositide 3-kinase, ras-binding (IPR000341)

Phosphatidylinositol 3- and 4-kinase, catalytic (IPR000403)

Phosphoinositide 3-kinase accessory region PIK (IPR001263)

Phosphoinositide 3-kinase, C2 (IPR002420)

Phosphatidylinositol 3-kinase, p85-binding (IPR003113)

C2 calcium/lipid-binding region, CaLB (IPR008973)

Protein kinase-like (IPR011009)

Phosphatidylinositol Kinase (IPR015433)

Armadillo-type fold (IPR016024)

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Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

P91634

Maps to

Does NOT map to

Identified with

SD05105

(BDGP Project Members, 2000-)

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Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

Pi3K92E[+t14.7]
3R:16,451,399..16,464,293

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-BamHI_rfrag

(Weinkove et al., 1999)

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External Data

DEDB - Drosophila exon database: splicing graphs

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10117

Crossreferences

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Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

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Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

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Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

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Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

detailed view

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External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0015279

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0015279

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0015279

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Alleles & Phenotypes

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Summary of Allele Phenotypes

Lethality

Allele

viable (with Pi3K92E^A)

Pi3K92E^2H1

viable (with Pi3K92E^2H1)

Pi3K92E^A

viable, with Scer\GAL4^Adh.PF

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

viable, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

Pi3K92E^unspecified

lethal | recessive

Pi3K92E^A

lethal, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

lethal, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

Other Phenotypes

Allele

Pi3K92E^+t14.7

visible | somatic clone

Pi3K92E^5W3

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^dpp.blk1

visible, with Scer\GAL4^ap-md544

visible, with Scer\GAL4^GMR.PF

size defective | cell autonomous, with Scer\GAL4^dpp.blk1

size defective | cell non-autonomous, with Scer\GAL4^dpp.blk1

size defective, with Scer\GAL4^ap-md544

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

small body (with Pi3K92E^A)

Pi3K92E^2H1

small body (with Pi3K92E^2H1)

Pi3K92E^A

small body | somatic clone

Pi3K92E^unspecified

small body | second instar larval stage, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

small body | third instar larval stage, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

small body | pupal stage, with Scer\GAL4^amn-c651

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

small body | pharate adult stage, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

small body, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

reduced cell number, with Scer\GAL4^dpp.blk1

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell number, with Scer\GAL4^GMR.PF

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell number, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

reduced cell size | recessive | somatic clone

Pi3K92E^A

reduced cell size, with Scer\GAL4^Bx-MS1096

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell size, with Scer\GAL4^dpp.blk1

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell size, with Scer\GAL4^ap-md544

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell size, with Scer\GAL4^GMR.PF

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

reduced cell size, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

large body | pupal stage, with Scer\GAL4^amn-c651

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

large body | pupal stage, with Scer\GAL4^amn-X8

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

large body | adult stage, with Scer\GAL4^amn-c651

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

large body, with Scer\GAL4^P0206

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

increased cell number, with Scer\GAL4^dpp.blk1

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

increased cell number, with Scer\GAL4^P0206

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

increased cell size, with Scer\GAL4^Bx-MS1096

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

increased cell size, with Scer\GAL4^dpp.blk1

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

environmental stress response defective, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

feeding behavior defective, with Scer\GAL4^Adh.PF

feeding behavior defective, with Scer\GAL4^NPFR1.6.6

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

photoperiod response variant, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

cell death decrease, with Scer\GAL4^hs.PB

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

cell growth defective | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

cell growth defective, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

cell size defective, with Scer\GAL4^amn-c651

cell size defective, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

hyperplastic, with Scer\GAL4^GMR.PF

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

hyperplastic, with Scer\GAL4^nub-AC-62

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

tissue polarity defective | recessive | somatic clone

Pi3K92E^A

tissue polarity defective | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

developmental rate defective | third instar larval stage, with Scer\GAL4^amn-c651

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

developmental rate defective | third instar larval stage, with Scer\GAL4^amn-X8

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

developmental rate defective, with Scer\GAL4^P0206

developmental rate defective, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

wound healing defective, with Scer\GAL4^crq.PO

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

Sterility

Allele

fertile, with Scer\GAL4^Adh.PF

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

fertile, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

Phenotype manifest in

Allele

head capsule | somatic clone

Pi3K92E^5W3

Pi3K92E^A

Pi3K92E^A

Pi3K92E^A

ommatidium | somatic clone

Pi3K92E^A

1st posterior cell, with Scer\GAL4^ptc-559.1

wing, with Scer\GAL4^Bx-MS1096

eye, with Scer\GAL4^Bx-MS1096

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

eye, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^GMR.PF

chaeta, with Scer\GAL4^ap-md544

mesothoracic tergum, with Scer\GAL4^ap-md544

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

1st posterior cell, with Scer\GAL4^dpp.blk1

wing, with Scer\GAL4^dpp.blk1

wing & cell, with Scer\GAL4^dpp.blk1

prothoracic gland, with Scer\GAL4^amn-c651

dorsal mesothoracic disc | posterior compartment, with Scer\GAL4^en-e16E

imaginal disc & cell, with Scer\GAL4^Act5C.PP

wing, with Scer\GAL4^P0206

eye, with Scer\GAL4^P0206

germ band, with Scer\GAL4^Kr.PM

Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}

fat body, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

mitotic cell cycle | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

ommatidial cluster | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

eye disc | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval fat body | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval digestive system | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval Malpighian tubule | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval epidermis | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

cell surface & nurse cell | somatic clone, with Scer\GAL4^Act5C.PP

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

ring canal, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

prothoracic gland, with Scer\GAL4^P0206

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

interommatidial bristle, with Scer\GAL4^GMR.PF

wing, with Scer\GAL4^ap-md544

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval salivary gland, with Scer\GAL4^29BD

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

dorsal mesothoracic disc, with Scer\GAL4^29BD

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^αTub84B.PC

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

prothoracic gland, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

puparium, with Scer\GAL4^phm.PO

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

lysosome, with Scer\GAL4^Lsp2.PH

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

autophagic vacuole, with Scer\GAL4^Lsp2.PH

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval salivary gland, with Scer\GAL4^hs.PB

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval salivary gland, with Scer\GAL4^nub-AC-62

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

larval salivary gland, with Scer\GAL4^fkh.PH

Pi3K92E^{Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

wing | posterior compartment, with Scer\GAL4^en-e16E

Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}

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Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Pi3K92E	Class	Mutagen	Stocks	Known lesion
Pi3K92E^2H1			0	--
Pi3K92E^5W3			0	--
Pi3K92E^A		P-element activity	0	Yes
Pi3K92E^e03435		piggyBac transposase	1	--
Pi3K92E^unspecified			0	--

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Alleles Carried on Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Pi3K92E	Class	Mutagen	Stocks	Known lesion
Pi3K92E^+t14.7		in vitro construct \| genomic fragment	0	Yes
Pi3K92E^A2860C.GMR	antimorph	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	2	Yes
Pi3K92E^{A2860C.Scer\UAS}	antimorph	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	2	Yes
Pi3K92E^ACT.GMR		in vitro construct \| regulatory fusion	0	Yes
Pi3K92E^CAAX.GMR	gain of function	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	1	Yes
Pi3K92E^{CAAX.Scer\UAS}	gain of function	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	1	Yes
Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}		in vitro construct \| site directed mutagenesis in vitro construct \| amino acid replacement	0	Yes