A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Ost48

General Information
SymbolDmel\Ost48SpeciesD. melanogaster
NameOligosaccharyltransferase 48kD subunitAnnotation symbolCG9022
Feature typeprotein_coding_geneFlyBase IDFBgn0014868
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)XRecombination map1-
Cytogenetic map8C14-8C14Sequence locationX:9,050,955..9,052,544 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Oligosaccharyltransferase 48kD subunit is referred to in FlyBase by the symbol Ost48 (CG9022, FBgn0014868). It has the cytological map location 8C14. Its sequence location is X:9050955..9052544. Its molecular function is described as dolichyl-diphosphooligosaccharide-protein glycotransferase activity. It is involved in the biological processes: protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine. One allele is reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
8C14-8C14  
Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912EP912  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
1-
 
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: Ost48? His3.3B+
(Akhmanova et al., 1995)
Overall orientation not stated: His3.3B- Ost48- CG9034- Zpr1- mei-P26+
(Page et al., 2000)
hide Gene Model & Products
Please see the GBrowse view of Dmel\Ost48 for information on other features
detailed view FBtr0071346 FBtr0071347 FBtr0071345 FBtr0071344 FBtr0071302 FBtr0071343 FBtr0071342 FBpp0071279 FBpp0071237 FBpp0071278 FBpp0071277 FBti0055401 FBti0071362 FBti0035569 FBti0072014 FBti0007514 FBti0024116 FBti0007399 FBti0029176
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Ost48-RA
FBtr0071344
 NM_078541
  1516
  449
Additional Transcript Data & Comments
Reported size (kB)
1.480 (unknown)
(Stagljar et al., 1995)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Ost48-PA  
FBpp0071279  
50.0  
449  
5.31  
  NP_511096
  AAF46453
Additional Polypeptide Data & Comments
Reported size (kD)
330 (aa)
(Akhmanova et al., 1995)
449 (aa); 50 (kD predicted)
(Stagljar et al., 1995)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit (IPR005013)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA390685
 
AA391368
 
AA438484
 
AA539212
 
AA540479
 
AA695000
 
AA697018
 
AA801934
 
AA816530
 
AA816989
 
AE003446
AAF46453
 
AI124253
 
AI455026
 
AI515624
 
AW942621
 
BF485528
 
BF487805
 
BF492182
 
BF496372
 
BF498931
 
BG637734
 
BI168393
 
BI213875
 
BI238528
 
BI239467
 
BI242944
 
BI356672
 
BI578196
 
BI590156
 
BP542079
 
BP550638
 
BT010050
AAQ22519
 
CO155915
 
CO268714
 
CO297486
 
CO299158
 
CO311302
 
CO313353
 
CO320116
 
EC064551
 
EC085335
 
EC087488
 
EC202213
 
EC225642
 
EC262978
 
LD02021
 
X81207
CAA57079
 
X81999
CAA57525
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0071346 FBtr0071347 FBtr0071345 FBtr0071344 FBtr0071302 FBtr0071343 FBtr0071342
        AA816989
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Ost48
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for Ost48
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 0 )
        For All Classical Alleles Show

        Allele of Ost48ClassMutagenStocksKnown lesion
        hide Alleles Carried on Transgenic Constructs ( 1 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Ost48ClassMutagenStocksKnown lesion
        Ost48GD32905 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD3290}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Ost48 is cloned and sequenced, structural features of the cDNA are determined.
        (Stagljar et al., 1995)
        hide Gene Ontology: Function, Process & Cellular Component ( 5 )
        hide Molecular Function
        CV term
        Evidence
        References
        dolichyl-diphosphooligosaccharide-protein glycotransferase activity
        inferred from sequence or structural similarity with NCBI_gi:627424
        (FlyBase, 1992-)
        hide Biological Process
        CV term
        Evidence
        References
        protein amino acid N-linked glycosylation
        non-traceable author statement
        (Swiss-Prot Project Members, 1997.2.1)
        protein amino acid N-linked glycosylation via asparagine
        inferred from electronic annotation with InterPro:IPR005013
        (FlyBase Curators et al., 2004-)
        hide Cellular Component
        CV term
        Evidence
        References
        endoplasmic reticulum membrane
        inferred from electronic annotation with InterPro:IPR005013
        (FlyBase Curators et al., 2004-)
        lipid particle
        inferred from direct assay
        (Cermelli et al., 2006)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Ost48 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 5 )
        VDRC
        v5509
        w1118 P{GD3290}v5509
        v45171
        w1118; P{GD3290}v45171
        v45172
        w1118; P{GD3290}v45172
        v45941
        w1118; P{GD3290}v45941
        v45173
        w1118; P{GD3290}v45173/CyO
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 32 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for identity of: Ost48 CG9022
        (Beasley, 1999.11)
        Source for database merge of
        Source for merge of: Ost48 anon-EST:Liang-1.11
        (Ashburner, 2004.6.30)
        Additional comments
        hide Comments About Role
        hide Comments About Molecular Function
        hide Other Comments
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/Swiss-Prot
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit (IPR005013)
        Linkouts
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Ost48
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DEDB - Drosophila exon database: splicing graphs
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        FlyExpress - Embryonic expression images (BDGP data)
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        InParanoid orthologs - Eukaryotic orthologs
        •
        PANTHER - Protein classification by function, families, and pathways
        •
        hide Synonyms & Secondary IDs ( 11 )
        Reported As
        Symbol Synonym
        anon-EST:Liang-1.11
         
        CG9022
        (Ashburner, 2004.6.30, Cermelli et al., 2006)
        clone 1.11
        (Liang and Biggin, 1998)
        DmOST50
        (Stagljar et al., 1995, )
        DrOST
        (Stagljar, 1994.9.30, )
        OST48
        (Akhmanova et al., 1995)
        Ost48
        (Cermelli et al., 2006, Stuart et al., 2007)
        OST48/WBP1
        (Fabini and Wilson, 2001)
        Ots48
        (Foley and O'Farrell, 2004)
        Name Synonym
        oligosaccaryltransferase 48kDa subunit
        (Cermelli et al., 2006)
        Oligosaccharyltransferase 48kD subunit
         
        Secondary FlyBase IDs
        • FBgn0026008
        hide References ( 19 )
        Research paper
        Stuart et al., 2007, Nature 445(7123): 95--101
        A systems biology analysis of the Drosophila phagosome. [FBrf0195342]
        Cermelli et al., 2006, Curr. Biol. 16(18): 1783--1795
        The lipid-droplet proteome reveals that droplets are a protein-storage depot. [FBrf0192764]
        Glasscock et al., 2005, Genetics 170(4): 1677--1689
        The mei-P26 Gene Encodes a RING Finger B-box Coiled-Coil-NHL Protein That Regulates Seizure Susceptibility in Drosophilia. [FBrf0187640]
        Foley and O'Farrell, 2004, PLoS Biol. 2(8): 1091--1106
        Functional dissection of an innate immune response by a genome-wide RNAi screen. [FBrf0179207]
        Fabini and Wilson, 2001, D. I. S. 84: 122--129
        Glycosyltransferases in Drosophila melanogaster. [FBrf0150727]
        Page et al., 2000, Genetics 155(4): 1757--1772
        Genetic studies of mei-P26 reveal a link between the processes that control germ cell proliferation in both sexes and those that control meiotic exchange in Drosophila. [FBrf0130005]
        Liang and Biggin, 1998, Development 125(22): 4471--4482
        Eve and ftz regulate a wide array of genes in blastoderm embryos: the selector homeoproteins directly or indirectly regulate most genes in Drosophila. [FBrf0105875]
        Akhmanova et al., 1995, Genome 38(3): 586--600
        Structure and expression of histone H3.3 genes in Drosophila melanogaster and Drosophila hydei. [FBrf0082998]
        Stagljar et al., 1995, Gene 158(2): 209--212
        PCR-mediated cloning and sequencing of the DmOST50 gene, a WBP1/AvOST50/OST48 homologue, from Drosophila melanogaster. [FBrf0082636]
        Supplementary material
        Giot et al., 2003, Science 302(5651):
        Tables S1 to S7. [FBrf0166452]
        Personal communication to FlyBase
        Dickson et al., 2007.7.18, RNAi construct and in