Gene Dmel\Mcm2

(BDGP Project Members, 1994-1999)

84F6-84F7

(determined by in situ hybridisation)

(Spradling et al., 1999)

84F6-84F7

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: anon-84Fa- Mcm2+ anon-84Fb?

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Mcm2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Mcm2-RA

FBtr0081827

NM_057773

3032

887

Additional Transcript Data & Comments

Reported size (kB)

2.8 (compiled cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Mcm2-PA

FBpp0081317

100.4

887

4.74

NP_477121

AAF54207

Additional Polypeptide Data & Comments

Reported size (kD)

887 (aa)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0014861

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

MCM (IPR001208)

MCM protein 2 (IPR008045)

Nucleic acid-binding, OB-fold (IPR012340)

Nucleic acid-binding, OB-fold-like (IPR016027)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P49735

UniProtKB/TrEMBL

Maps to

FBcl0298006

(BDGP Project Members, 2000-)

Does NOT map to

Identified with

LD05520

(Spradling et al., 1999)

LD47441

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

8605

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | early

embryonic stage

central nervous system

embryonic stage

embryonic/larval digestive system

embryonic stage

embryonic peripheral nervous system

embryonic stage | stage 8

embryonic stage

Malpighian tubule

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA817224
AI515560

External Data & Images

Show all FlyExpress processed images for Mcm2

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0014861

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0014861

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0014861

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Mcm2^rL074

lethal | adult stage | recessive

Mcm2^rL074

Phenotype manifest in

Allele

nuclear chromosome

Mcm2^dsRNA.cCa

central nervous system

Mcm2^rL074

imaginal disc

Mcm2^rL074

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Mcm2	Class	Mutagen	Stocks	Known lesion
Mcm2^rL074		P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Mcm2	Class	Mutagen	Stocks	Known lesion
Mcm2^GD4467		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Mcm2^dsRNA.cCa		in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)CA3

Df(3R)p712

Useful duplication

Dp(3;3)D1

Disrupted in

Df(3R)ED5296

(Ryder and Roote, 2004.11.10)

Df(3R)Exel6147

(Ryder, 2004.4.26)

Df(3R)p712

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4467}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{PZ}Mcm2^rL074

Yes

Related Comments

Please look at the allele reports for the complete phenotype data

Mcm2 plays a role in the regulation of DNA replication analogous to that of its yeast counterpart (MCM2).

(FlyBase Curators et al., 2004-)

Gene Ontology: Function, Process & Cellular Component ( 11 )

Molecular Function

CV term

Evidence

References

contributes_to
3'-5' DNA helicase activity

inferred from direct assay

(Moyer et al., 2006)

ATP binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008045

DNA binding

non-traceable author statement

(FlyBase Curators et al., 2004-)

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008045

chromatin binding

inferred from sequence or structural similarity with SGD_LOCUS:MCM2; SGD:S0000119

non-traceable author statement

(Lee and Orr-Weaver, 2003)

Biological Process

CV term

Evidence

References

DNA replication

traceable author statement

non-traceable author statement

DNA replication initiation

inferred from electronic annotation with InterPro:IPR008045

(FlyBase Curators et al., 2004-)

cell proliferation

non-traceable author statement

(Christensen and Tye, 2003)

chromosome condensation

inferred from mutant phenotype

pre-replicative complex assembly

inferred from sequence or structural similarity with SGD_LOCUS:MCM2; SGD:S0000119

non-traceable author statement

Cellular Component

CV term

Evidence

References

nucleus

non-traceable author statement

pre-replicative complex

inferred from sequence or structural similarity with SGD_LOCUS:MCM2; SGD:S0000119

non-traceable author statement

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Mcm2 allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66154

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18817

Dere\GG24143

Dgri\GH14181

Dmoj\GI22091

Dper\GL23360

Dpse\GA20424

Dsec\GM23713

Dsim\GD18528

Dvir\GJ24206

Dwil\GK11732

Dyak\GE25859

Curated drosophilid orthologs

Dpse\GA20424

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013357
Anopheles gambiae (Ensembl)
ENSBTAP00000019121
Bos taurus (Ensembl)
ENSCAFP00000006027
Canis familiaris (Ensembl)
ENSCINP00000016052
Ciona intestinalis (Ensembl)
NEWSINFRUP00000163970
Takifugu rubripes (Ensembl)
ENSGALP00000009709
Gallus gallus (Ensembl)
ENSP00000265056
Homo sapiens (Ensembl)
ENSMMUP00000000221
Macaca mulatta (Ensembl)
ENSMODP00000009819
Monodelphis domestica (Ensembl)
ENSPTRP00000026448
Pan troglodytes (Ensembl)
ENSRNOP00000022231
Rattus norvegicus (Ensembl)
GSTENP00032473001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000004403
Xenopus tropicalis (Ensembl)
Q6QNH2
Oryza sativa (Gramene)
AT1G44900.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037858
Caenorhabditis briggsae (Wormbase)
WBGene00003154
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-509
Danio rerio (Zfin)
ZDB-CDNA-040425-510
Danio rerio (Zfin)
ZDB-GENE-020419-24
Danio rerio (Zfin)
DDB0232353
Dictyostelium discoideum (Dictybase)
MGI:105380
Mus musculus (Informatics)
YBL023C
Saccharomyces cerevisiae (Yeastgenome)
SPBC4.04c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v10967

w¹¹¹⁸; P{GD4467}v10967/TM3

Bloomington

12122

ry⁵⁰⁶ P{PZ}Mcm2^rL074/TM3, Sb¹

Genomic Clones ( 1 )

BACR45A07

cDNA Clones ( 44 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD47441

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0014861

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0014861

Antibody Information

Other Information

Discoverer

G. Rubin.

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Mcm2 CG7538

(Zhao, 1999.11)

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

(Moyer et al., 2006)

Other Comments

For the yeast genes MCM stands for 'minichromosome maintenance'.