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Gene Dmel\mof

General Information
Symbol	Dmel\mof	Species	D. melanogaster
Name	males absent on the first	Annotation symbol	CG3025
Feature type	protein_coding_gene	FlyBase ID	FBgn0014340
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	5C5-5C5	Sequence location	X:5,767,868..5,770,884 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene males absent on the first is referred to in FlyBase by the symbol mof (CG3025, FBgn0014340). It has the cytological map location 5C5. Its sequence location is X:5767868..5770884. Its molecular function is described as: histone acetyltransferase activity; lysine N-acetyltransferase activity; H3/H4 histone acetyltransferase activity; mRNA binding; chromatin binding; histone acetyltransferase activity (H4-K16 specific); protein binding. It is involved in the biological processes: dosage compensation; histone acetylation; peptidyl-lysine acetylation; dosage compensation, by hyperactivation of X chromosome; chromatin assembly or disassembly. 21 alleles are reported. The phenotype of these alleles is annotated with embryonic heart. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 5C5-5C5   Limits computationally determined from genome sequence between P{EP}CG15770EP390 and P{EP}Act5CEP1604&P{EP}EP444
Experimentally Determined Cytological Location
Cytogenetic map Notes References 5C3-5C3   (Hilfiker, 1996.9.18) 5C3-5C3 (determined by in situ hybridisation)   (Hilfiker et al., 1997)
Experimentally Determined Recombination Data
Location	1-13.8 (Hilfiker et al., 1995, Hilfiker et al., 1998)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: anon-5Ca? anon-5Cb? mof? anon-5Cc? (Hilfiker et al., 1997)
Gene Model & Products
Please see the GBrowse view of Dmel\mof for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mof-RA FBtr0070829  NM_078496   3017   827
Additional Transcript Data & Comments
Reported size (kB)	3.2 (northern blot) (Hilfiker et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein mof-PA   FBpp0070794   92.7   827   4.42     NP_511051   AAF46095
Additional Polypeptide Data & Comments
Reported size (kD)	827 (aa) (Hilfiker et al., 1997)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0014340
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • MOZ/SAS-like protein (IPR002717) Winged helix repressor DNA-binding (IPR011991) Chromo domain (IPR000953) Acyl-CoA N-acyltransferase (IPR016181)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA820460   AA949216   AA951642   AA951643   AA952138   AE003435 AAF46095   AI455435   AI515736   AI520121   AI532144   AI542271   AY102685 AAM27514   BI162284   BI168245   BI172214   BI215011   BI227575   BI229771   BI233039   BI233059   BI234260   BI236685   BI363085   BI483192   BI483590   BI483606   BI580230   BI641971   CO264242   CO277135   CO284373   CO319221   CO319826   CO329340   DN154184   EC067080   EC195506   EC197744   EC211162   EC216174   EC239726   EC250430   U71219 AAC47507
UniProtKB/Swiss-Prot	O02193
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	LD24203 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation mof[2] X:5,769,675..5,769,675 comment=Site of nucleic acid change inferred from report that a truncated protein of 370 amino acids is made in the mutant. evidence=experimental na_change=C5720878T pr_change=Q371@|mof-PA (Gu et al., 1998) point mutation mof[1] X:5,768,714..5,768,714 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G5719917A pr_change=G691E|mof-PA reported_pr_change=G691E (Hilfiker et al., 1997) rescue fragment mof[+t6.8] X:5,766,109..5,773,204 comment=Rescues the male lethal phenotype. linked_to=HindIII-HindIII_rfrag (Hilfiker et al., 1997)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 589
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  larval stage-adult stage   (Hilfiker et al., 1997)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014340 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0014340 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0014340
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable | female mof1 lethal | male | recessive mof1 Other Phenotypes Allele wild-type mof+t10.8 mof+t18 mof+t6.8 non-suppressor of variegation, with P{UAS-lacZ.Abd-B.5F24}25,2a mofunspecified Phenotype manifest in Allele embryonic heart mofdsRNA.cKa mofdsRNA.si.cKa
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of mof Class Mutagen Stocks Known lesion mof1 ethyl methanesulfonate 0 Yes mof2 0 Yes mofunspecified 0 --
Alleles Carried on Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mof Class Mutagen Stocks Known lesion mof+t10.8 in vitro construct | genomic fragment 0 Yes mof+t18 in vitro construct | genomic fragment 0 Yes mof+t6.8 in vitro construct | genomic fragment 0 Yes mof1.MtnA.T:Ivir\HA1 in vitro construct | amino acid replacement 0 Yes mofGD10025 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes mofL578G.Y580G.hs.T:Avic\GFP-EGFP in vitro construct | amino acid replacement 0 Yes mofMtnA.T:Ivir\HA1 in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes mofMtnA.cAa.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes mofW426G.MtnA.T:Ivir\HA1 in vitro construct | amino acid replacement 0 Yes mofY416D.MtnA.T:Ivir\HA1 in vitro construct | amino acid replacement 0 Yes mofY416D.hs.T:Avic\GFP-EGFP in vitro construct | amino acid replacement 0 Yes mofdsRNA.cBa in vitro construct | RNAi 0 Yes mofdsRNA.cKa in vitro construct | RNAi 0 Yes mofdsRNA.si.cKa in vitro construct | RNAi 0 Yes mofhs.PS in vitro construct | regulatory fusion 0 Yes mofhs.T:Avic\GFP-EGFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes moftHa in vitro construct | genomic fragment 0 Yes moftub.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)N73
Disrupted in	Df(1)Exel6237 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10025} NA characterization construct P{mof+.HH} NA P{mof+.SS} NA P{mof+.XB} NA P{mof+.XK} NA heat-shock construct P{hs-mof} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Male lethal locus that exhibits a strong maternal effect and is responsible for dosage compensation in males. (Hilfiker et al., 1995) mof encodes a putative histone acetyl transferase (HAT) that plays a direct role in the specific histone acetylation associated with dosage compensation, the male-specific hypertranscription of X-linked genes. mof function in dosage compensation is supported by the effect of the mutation on the binding of other dosage compensation regulatory factors to the X chromosome and its effect on the normal consequences that this binding has on nucleosomal structure. (Hilfiker et al., 1997)
Gene Ontology: Function, Process & Cellular Component ( 16 )
Molecular Function
CV term Evidence References
H3/H4 histone acetyltransferase activity traceable author statement (Amrein, 2000) traceable author statement (Wutz, 2003)
chromatin binding traceable author statement (Akhtar, 2003) inferred from electronic annotation with InterPro:IPR000953 (FlyBase Curators et al., 2004-)
histone acetyltransferase activity inferred from sequence or structural similarity with SGD_LOCUS:SAS2; SGD:S0004734 AND inferred from sequence or structural similarity with SGD_LOCUS:SAS3; SGD:S0000148 (Hilfiker et al., 1997) non-traceable author statement (Hilfiker, 1996.9.18) traceable author statement (Carrozza et al., 2003) inferred from sequence or structural similarity with SGD_LOCUS:ESA1; SGD:S0005770 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1998.2.1)
histone acetyltransferase activity (H4-K16 specific) traceable author statement (Akhtar, 2003) traceable author statement (Brehm et al., 2004)
lysine N-acetyltransferase activity traceable author statement (Carrozza et al., 2003)
mRNA binding traceable author statement (Amrein, 2000) traceable author statement (Akhtar, 2003) traceable author statement (Wutz, 2003)
protein binding traceable author statement (Akhtar, 2003)
Biological Process
CV term Evidence References
chromatin assembly or disassembly inferred from electronic annotation with InterPro:IPR000953 (FlyBase Curators et al., 2004-)
dosage compensation inferred from genetic interaction with FLYBASE:msl-2; FB:FBgn0005616 AND inferred from mutant phenotype (Hilfiker et al., 1997) non-traceable author statement (Swiss-Prot Project Members, 1998.2.1) traceable author statement (Akhtar, 2003)
dosage compensation, by hyperactivation of X chromosome non-traceable author statement (FlyBase, 1992-) non-traceable author statement (Schutt and Nothiger, 2000)
histone acetylation traceable author statement (Carrozza et al., 2003) non-traceable author statement (Swiss-Prot Project Members, 1998.2.1) traceable author statement (Amrein, 2000) traceable author statement (Akhtar, 2003)
peptidyl-lysine acetylation traceable author statement (Carrozza et al., 2003) traceable author statement (Akhtar, 2003)
Cellular Component
CV term Evidence References
X chromosome traceable author statement (Brehm et al., 2004)
X chromosome located dosage compensation complex, transcription activating non-traceable author statement (FlyBase, 1992-) non-traceable author statement (Schutt and Nothiger, 2000) traceable author statement (Amrein, 2000) non-traceable author statement (Wutz, 2003)
chromatin inferred from electronic annotation with InterPro:IPR000953 (FlyBase Curators et al., 2004-)
nucleus inferred from electronic annotation with InterPro:IPR000953 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mof allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58017
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21061 Dere\GG18463 Dgri\GH11980 Dmoj\GI14721 Dper\GL15211 Dpse\GA15730 Dsec\GM12611 Dvir\GJ16786 Dwil\GK16378 Dyak\GE15686
Curated drosophilid orthologs	Dpse\GA15730   Dsim\mof (Pal Bhadra et al., 2006)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000016550 Anopheles gambiae (Ensembl) ENSBTAP00000013893 Bos taurus (Ensembl) ENSCAFP00000024770 Canis familiaris (Ensembl) NEWSINFRUP00000129410 Takifugu rubripes (Ensembl) ENSP00000219797 Homo sapiens (Ensembl) ENSMODP00000002268 Monodelphis domestica (Ensembl) ENSRNOP00000026527 Rattus norvegicus (Ensembl) GSTENP00021290001 Tetraodon nigroviridis (Ensembl) ENSXETP00000004144 Xenopus tropicalis (Ensembl) WBGene00038164 Caenorhabditis briggsae (Wormbase) WBGene00010537 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-3716 Danio rerio (Zfin) ZDB-GENE-030131-7510 Danio rerio (Zfin) MGI:1915023 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 1 )
	BACR11C13
cDNA Clones ( 31 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE08841 RE14509 RE66044 RE01649 SD25664 RE29335 RE27622 LD28029 RE29935 RE66507 RE24664 LD40125 LD29211 SD08503 RE74383 LD32280 LD32279 LD41389 RE47811 RE21191 RE32778 RE66523 RE29323 SD03525
Other clones	EK276760 EK280957 EK165919 EK138304 EK292633 EK123134 EK193610
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0014340 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0014340
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: mof CG3025 (Zhong, 1999.11)
Source for database merge of
Additional comments
Comments About Role
	mof colocalises with the MSL complex on the X chromosome: a sequence of binding events results in the formation of the MSL complex on the X chromosome in males and in the targeting of mof to its presumed site of action. (Gu et al., 1998) Gene products of the male specific lethal (msl) group of genes preferentially associate with the male X chromosome and may have a role in dosage compensation. This may be achieved by regulating an inverse dosage effect, which would be maintained on the male X and nullified on the autosomes. (Bhadra et al., 1999)
Comments About Molecular Function
	Gene products of the male specific lethal (msl) group of genes including msl-1, msl-2, msl-3, mle, and mof are associated with all female chromosomes at a low level but are sequestered to the X chromosome in males. There is evidence for the presence of nucleation sites for association of msl proteins with the X chromosome rather than individual gene binding sites. mof is still capable of associating with the chromosome in msl mutant individuals. It appears to be able to dissociate from other members of the MSL complex under these circumstances and act independently. (Bhadra et al., 1999)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078496 RefSeq (Proteins) • NP_511051 DDBJ / EMBL / GenBank • AA820460 AE003435 AAF46095 AI455435 AY102685 AAM27514 U71219 AAC47507 UniProtKB/Swiss-Prot • O02193
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • MOZ/SAS-like protein (IPR002717) Winged helix repressor DNA-binding (IPR011991) Chromo domain (IPR000953) Acyl-CoA N-acyltransferase (IPR016181)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58017 DEDB - Drosophila exon database: splicing graphs • 589 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0014340 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014340 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0014340 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0014340 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0014340 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0014340 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000016550 Anopheles gambiae (Ensembl) ENSBTAP00000013893 Bos taurus (Ensembl) ENSCAFP00000024770 Canis familiaris (Ensembl) NEWSINFRUP00000129410 Takifugu rubripes (Ensembl) ENSP00000219797 Homo sapiens (Ensembl) ENSMODP00000002268 Monodelphis domestica (Ensembl) ENSRNOP00000026527 Rattus norvegicus (Ensembl) GSTENP00021290001 Tetraodon nigroviridis (Ensembl)<