Gene Dmel\barr

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General Information

Symbol

Dmel\barr

Species

D. melanogaster

Name

barren

Annotation symbol

CG10726

Feature type

protein_coding_gene

FlyBase ID

FBgn0014127

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

38B1-38B2

Sequence location

2L:20,058,164..20,060,975 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene barren is referred to in FlyBase by the symbol barr (CG10726, FBgn0014127). It has the cytological map location 38B1-38B2. Its sequence location is 2L:20058164..20060975. Its molecular function is unknown. It is involved in the biological processes: mitosis; mitotic chromosome condensation; mitotic sister chromatid segregation; Malpighian tubule morphogenesis; ectodermal gut morphogenesis; head involution; morphogenesis of an epithelium; open tracheal system development; peripheral nervous system development; mitotic spindle organization and biogenesis. 17 alleles are reported. The phenotypes of these alleles are annotated with 11 unique terms, many of which group under: organ system; anatomical structure; peripheral nervous system; nervous system; organism; embryonic head; embryonic/larval tracheal system; embryonic nervous system; embryonic hindgut; midgut constriction; cell cycle. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38B1-38B2

Limits computationally determined from genome sequence between P{lacW}l(2)k10239^k10239 and P{lacW}barr^k14014

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

38B2-38B4

(Bhat, 1996.10.11)

38B1-38B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

38B1-38B2

(determined by in situ hybridisation)

(Bhat et al., 1996)

38A-38A

(determined by in situ hybridisation)

(Kania et al., 1995)

38A-38A

(determined by in situ hybridisation)

(Bhat and Bellen, 1995)

38A-38A

38B1--2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: barr- neb+

(Bhat et al., 1996)

In direction of increasing cytology: sNPF+ barr- lok+

(Butler et al., 2001)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: barr- lok+

(Xu et al., 2001)

Gene Model & Products

Please see the GBrowse view of Dmel\barr for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

barr-RA

FBtr0081348

NM_057758

2577

735

barr-RB

FBtr0081349

NM_165317

2681

735

Additional Transcript Data & Comments

Reported size (kB)

2.6 (northern blot)

(Bhat et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

barr-PA

FBpp0080880

82.8

735

5.26

NP_477106

AAF53866

barr-PB

FBpp0080881

82.8

735

5.26

NP_724240

AAN11061

Additional Polypeptide Data & Comments

Reported size (kD)

736 (aa); 97 (kD observed); 81 (kD predicted)

(Bhat et al., 1996)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0014127

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Barren (IPR008418)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DS00863

(FlyBase, 1992-, Butler et al., 2001)

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Dm4263

(Spradling et al., 1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

barr[+tXa]
2L:20,058,207..20,061,851

comment=Genomic construct containing barr sequences rescues barr[E51]; position of rescue fragment on reference sequence inferred by FlyBase curator.
evidence=experimental

(Xu et al., 2001)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8164

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | early

(Bhat et al., 1996)

Marker for

Subcellular Localization

CV Term

mitotic chromosome

(Bhat et al., 1996)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0014127

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0014127

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0014127

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for barr

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for barr

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

barr^EY01867

lethal | recessive

lethal | embryonic stage | recessive

Other Phenotypes

Allele

wild-type

enhancer of variegation | partially, with P{hsp26-pt-T}39C-72

barr^L305

enhancer of variegation, with In(1)w^m4h

barr^L305

non-enhancer of variegation, with P{hsp26-pt-T}118E-10

barr^L305

suppressor of variegation | dominant, with In(1)w^m4h

barr^L305

mitotic

barr^dsRNA.cSa

Sterility

Allele

fertile

barr^EY01867

Phenotype manifest in

Allele

peripheral nervous system

neuron

mitosis & nuclear chromosome

barr^dsRNA.cSa

mitotic cell cycle

barr^dsRNA.cYa

chromosome

barr^dsRNA.cYa

multidendritic neuron

barr^k00310

chordotonal organ | lateral

barr^k00310

embryonic peripheral nervous system

embryonic Malpighian tubule

barr^k14014

larval proventriculus & embryo

barr^k14014

midgut constriction

barr^k14014

embryonic head

barr^k14014

embryonic/larval trachea

barr^k14014

embryonic epidermis

barr^k14014

larval pharynx & embryo

barr^k14014

Classical Alleles ( 11 )

For All Classical Alleles Show

Allele of barr	Class	Mutagen	Stocks	Known lesion
barr^E51		Δ2-3	0	Yes
barr^EY01867		P-element activity	1	--
barr^L305	amorph	P-element activity	2	Yes
barr^be408		P-element activity	0	Yes
barr^k00310		P-element activity	1	Yes
barr^k04805		P-element activity	0	Yes
barr^k04912		P-element activity	0	Yes
barr^k08103		P-element activity	1	--
barr^k13903		P-element activity	0	Yes
barr^k14014		P-element activity	2	Yes
barr^k14028		P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of barr	Mutagen	Stocks	Known lesion
barr^+tMa	in vitro construct \| genomic fragment	0	Yes
barr^+tXa	in vitro construct \| genomic fragment	0	Yes
barr^GD12427	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
barr^dsRNA.cSa	in vitro construct \| RNAi	0	Yes
barr^dsRNA.cYa	in vitro construct \| RNAi	0	--
barr^hs.PB	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)TW150

Disrupted in

Df(2L)BSC258

(Christensen and Cook, 2007.3.22)

Df(2L)E51

(Xu et al., 2001)

Df(2L)Exel7077

(Ryder, 2004.4.26)

Df(2L)TE38A-1

(Bhat et al., 1996)

Df(2L)TW1

(Butler et al., 2001)

Df(2L)TW150

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)TW161

(Spradling et al., 1999, Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)TW65

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)TW84

(Liu et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999, Kania et al., 1995)

Df(2L)TW9

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)VA12

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)VA19

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)barr^k04805

(Bhat et al., 1996)

Df(2L)barr^k04912

(Bhat et al., 1996)

Df(2L)be408

(Butler et al., 2001)

Df(2L)pr-A14

(BDGP Project Members, 1994-1999, Kania et al., 1995)

Df(2L)pr-A16

(Bhat et al., 1996)

Df(2L)pr-A20

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)pr-M1

(Butler et al., 2001)

Df(2L)pr11163

(Butler et al., 2001)

Df(2L)pr1122

(Butler et al., 2001)

Df(2L)pr2b

(Butler et al., 2001)

Df(2L)pr37

(Butler et al., 2001)

Df(2L)pr40

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)pr49

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)pr8311

(Butler et al., 2001)

Df(2L)pr9201

(Butler et al., 2001)

Df(2L)pr9202

(Butler et al., 2001)

Df(2L)pr9204

(Butler et al., 2001)

Not disrupted in

Df(2L)DS5

(Butler et al., 2001)

Df(2L)DS6

(Butler et al., 2001)

Df(2L)DS9

(Butler et al., 2001)

Df(2L)Sd37

(Butler et al., 2001)

Df(2L)TW158

(Butler et al., 2001)

Df(2L)TW50

(Butler et al., 2001, BDGP Project Members, 1994-1999, Bhat et al., 1996)

Df(2L)pr1

(Butler et al., 2001)

Df(2L)pr11164

(Butler et al., 2001)

Df(2L)pr1123

(Butler et al., 2001)

Df(2L)pr7

(Butler et al., 2001)

Df(2L)pr9133

(Butler et al., 2001)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

P{barr^+tXa}

heat-shock construct

P{CaSpeR-hs-barr}

UAS construct

P{GD12427}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}barr^EY01867

miscellaneous insertions

P{?'lacW}barr^L305

insertion of enhancer trap

Yes

Yes

Yes

Yes

Yes

Related Comments

Please look at the allele reports for the complete phenotype data

Weak alleles of barr cause sterile adults with rough eyes and few bristles that die 3-4 days after eclosion. Embryos exhibit loss of some external sensory and multidendritic neurons in the dorsal cluster and most of the chordotonal neurons in the lateral cluster. Severe alleles cause substantial loss of neurons, fasciculation defects and disruptions in the CNS longitudinal tracts.

(Bhat and Bellen, 1995)

Mutations in barr cause a failure of chromosomes to segregate correctly at anaphase, resulting in massive anaphase bridging. barr is not necessary for cell cycle progression out of mitosis. barr encodes a novel, conserved protein that localises throughout chromosomes during mitosis. barr protein associates with Top2 protein and affects its activity, this suggests barr protein functions in chromatid decatenation during the metaphase-anaphase transition.

(Bhat et al., 1996)

Inactivation of barr using RNA-mediated interference in S2 cells results in extensive defects in chromosome condensation and disrupts chromatid segregation.

(Somma et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in defects in chromosome condensation and chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 12 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

Malpighian tubule morphogenesis

inferred from mutant phenotype