Gene Dmel\Rlc1

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General Information

Symbol

Dmel\Rlc1

Species

D. melanogaster

Name

Rlc1

Annotation symbol

CG9378

Feature type

protein_coding_gene

FlyBase ID

FBgn0014023

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

85D19-85D19

Sequence location

3R:5,340,538..5,341,829 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Rlc1 (CG9378, FBgn0014023). It has the cytological map location 85D19. Its sequence location is 3R:5340538..5341829. Its molecular function is described as structural constituent of ribosome. It is involved in the biological process translation. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85D19-85D19

Limits computationally determined from genome sequence between P{PZ}ps¹⁰⁶¹⁵&P{PZ}Ras85D⁰⁶⁶⁷⁷ and P{EP}CG9393^EP3122

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

85D-85D

(Lev, 1993.6.4)

Experimentally Determined Recombination Data

Location

(Lev, 1993.6.4)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Rlc1+ Rlb1- Ras85D+

(Ezer et al., 1994, Schnorr and Berg, 1996)

Gene Model & Products

Please see the GBrowse view of Dmel\Rlc1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Rlc1-RA

FBtr0082120

NM_057752

989

227

Rlc1-RB

FBtr0082121

NM_176433

1110

271

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Rlc1-PA

FBpp0081598

26.4

227

10.41

NP_477100

AAF54390

Rlc1-PB

FBpp0081599

31.7

271

10.55

NP_788610

AAO41525

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0014023

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L47, mitochondrial (IPR010729)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

FBcl0298018

(FlyBase, 1992-)

Does NOT map to

Identified with

AT29239

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

Rlc1[tSa]
3R:5,336,635..5,342,154

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=EcoRI-EcoRI_rfrag

(Schnorr and Berg, 1996)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8817

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0014023

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0014023

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0014023

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Rlc1^EY10122

Sterility

Allele

fertile

Rlc1^EY10122

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of Rlc1	Mutagen	Stocks	Known lesion
Rlc1^EP3045	P-element activity	0	Yes
Rlc1^EP703	P-element activity	0	Yes
Rlc1^EY10122	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Rlc1	Class	Mutagen	Stocks	Known lesion
Rlc1^GD12554		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Rlc1^tSa		in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)by416

Useful duplication

Dp(3;3)M86D⁺2

Disrupted in

Df(3R)Exel6153

(Ryder, 2004.4.26)

Df(3R)ΔC17b

(Schnorr and Berg, 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12554}

characterization construct

P{R5.5ΔRas1}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}CG8176^EY10122

P{EP}CG8165^EP3045

P{EP}CG8165^EP703

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

structural constituent of ribosome

inferred from sequence or structural similarity with UniProtKB:Q9HD33

(Koc et al., 2001)

Biological Process

CV term

Evidence

References

translation

inferred from electronic annotation with InterPro:IPR010729

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

mitochondrial large ribosomal subunit

inferred from sequence or structural similarity with UniProtKB:Q9HD33

(Koc et al., 2001)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Rlc1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66302

Orthologs

Genome-wide drosophilid orthologs

Dana\GF16056

Dere\GG17365

Dgri\GH19393

Dmoj\GI22598

Dper\GL22226

Dpse\GA27426

Dsec\GM26250

Dsim\GD20790

Dvir\GJ10316

Dwil\GK13453

Dyak\GE24770

Curated drosophilid orthologs

Dpse\GA21743

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013554
Anopheles gambiae (Ensembl)
ENSBTAP00000003195
Bos taurus (Ensembl)
ENSCAFP00000016748
Canis familiaris (Ensembl)
ENSCINP00000003638
Ciona intestinalis (Ensembl)
NEWSINFRUP00000148718
Takifugu rubripes (Ensembl)
ENSGALP00000014750
Gallus gallus (Ensembl)
ENSP00000259038
Homo sapiens (Ensembl)
ENSMMUP00000012755
Macaca mulatta (Ensembl)
ENSMODP00000026628
Monodelphis domestica (Ensembl)
ENSPTRP00000026929
Pan troglodytes (Ensembl)
ENSRNOP00000015863
Rattus norvegicus (Ensembl)
GSTENP00028930001
Tetraodon nigroviridis (Ensembl)
Q658I1
Oryza sativa (Gramene)
AT1G07830.1
Arabidopsis thaliana (Arabidopsis)
WBGene00033727
Caenorhabditis briggsae (Wormbase)
WBGene00015092
Caenorhabditis elegans (Wormbase)
MGI:1921850
Mus musculus (Informatics)
SPCC4G3.06c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

v28130

w¹¹¹⁸; P{GD12554}v28130

Bloomington

20061

y¹ w^67c23; P{EPgy2}CG8176^EY10122 Rlc1^EY10122 CG8165^EY10122

Genomic Clones ( 2 )

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT29239

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0014023

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0014023

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Rlc1 BcDNA:AT29239 Source for merge of Rlc1 BcDNA:AT29239 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L47, mitochondrial (IPR010729)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66302

DEDB - Drosophila exon database: splicing graphs

•

8817

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0014023

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0014023

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0014023

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0014023

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0014023

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0014023

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013554
Anopheles gambiae (Ensembl)
ENSBTAP00000003195
Bos taurus (Ensembl)
ENSCAFP00000016748
Canis familiaris (Ensembl)
ENSCINP00000003638
Ciona intestinalis (Ensembl)
NEWSINFRUP00000148718
Takifugu rubripes (Ensembl)
ENSGALP00000014750
Gallus gallus (Ensembl)
ENSP00000259038
Homo sapiens (Ensembl)
ENSMMUP00000012755
Macaca mulatta (Ensembl)
ENSMODP00000026628
Monodelphis domestica (Ensembl)
ENSPTRP00000026929
Pan troglodytes (Ensembl)
ENSRNOP00000015863
Rattus norvegicus (Ensembl)
GSTENP00028930001
Tetraodon nigroviridis (Ensembl)
Q658I1
Oryza sativa (Gramene)
AT1G07830.1
Arabidopsis thaliana (Arabidopsis)
WBGene00033727
Caenorhabditis briggsae (Wormbase)
WBGene00015092
Caenorhabditis elegans (Wormbase)
MGI:1921850
Mus musculus (Informatics)
SPCC4G3.06c
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0014023

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

BcDNA:AT29239

CG9378

MRP-L47

(Koc et al., 2001)

mRpL47

Rlc1

Name Synonym

Rlc1

Secondary FlyBase IDs

FBgn0063700

References ( 13 )

Research paper

Koc et al., 2001, J. Biol. Chem. 276(47): 43958--43969: The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present. [FBrf0145610]
Schnorr et al., 2001, Genetics 159(2): 609--622: Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesis. [FBrf0139680]
Schnorr and Berg, 1996, Genetics 144(4): 1545--1557: Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis. [FBrf0091163]
Ezer et al., 1994, Dev. Dynamics 201(2): 179--190: Differential expression during embryogenesis of three genes clustered in the Ras1 region of Drosophila melanogaster. [FBrf0080012]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Lev, 1993.6.4, GenBank/EMBL/DDBJ: X73219: D. melanogaster genes Ras1, Rlb1 and Rlc1. [FBrf0117401]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]