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Gene Dmel\Nsf2

General Information
Symbol	Dmel\Nsf2	Species	D. melanogaster
Name	NEM-sensitive fusion protein 2	Annotation symbol	CG33101
Feature type	protein_coding_gene	FlyBase ID	FBgn0013998
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	87F15-87F15	Sequence location	3R:9,660,195..9,663,708 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene NEM-sensitive fusion protein 2 is referred to in FlyBase by the symbol Nsf2 (CG33101, FBgn0013998). It has the cytological map location 87F15. Its sequence location is 3R:9660195..9663708. Its molecular function is described as: ATP binding; nucleoside-triphosphatase activity. It is involved in the biological processes: neurotransmitter secretion; synaptic vesicle priming; ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; vesicle-mediated transport. 15 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; larval neuromuscular junction; eye. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87F15-87F15   Limits computationally determined from genome sequence between P{PZ}flfl01949 and P{lacW}trxj14A6&P{PZ}trx00347
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87F-87F Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000) 87F12-88A3   (Trimble, 1995.6.8)
Experimentally Determined Recombination Data
Location	3- (Trimble, 1995.6.8)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Nsf2 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nsf2-RA FBtr0082883  NM_176499   2540   752
Additional Transcript Data & Comments
Reported size (kB)	3.4, 3.0, 2.8, 2.4 (northern blot) (Pallanck et al., 1995) 2.4 (northern blot) (Boulianne and Trimble, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Nsf2-PA   FBpp0082346   83.4   752   6.71     NP_788676   AAF54995
Additional Polypeptide Data & Comments
Reported size (kD)	752 (aa) (Pallanck et al., 1995) 744 (aa) (Boulianne and Trimble, 1995)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0013998
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AAA ATPase VAT, N-terminal (IPR003338) AAA+ ATPase, core (IPR003593) AAA ATPase, core (IPR003959) AAA ATPase, conserved site (IPR003960) Cell division protein 48, CDC48, domain 2 (IPR004201) Aspartate decarboxylase-like fold (IPR009010)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392496   AA440279   AA540014   AA698578   AA698904   AA951209   AE003702 AAF54995   AI109834   BE975266   BF491742   BF498981   BI164019   BI166577   BI212528   BI212540   BI213072   BI213531   BI229700   BI230194   BI354200   BI355719   BI374309   BI576916   BI586893   BI630136   BP543811   BP543812   BT023784   CO154277   CO188422   CO278157   CO280530   CO322407   EC060706   EC061836   EC087042   EC087881   EC198279   EC234997   EC260956   U28836 AAC46844   U30502 AAA75044
UniProtKB/Swiss-Prot	P54351
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation Nsf2[102] 3R:9,663,329..9,663,329 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9663329T pr_change=S69L|Nsf2-PA reported_pr_change=S69L (Golby et al., 2001) point mutation Nsf2[55] 3R:9,663,147..9,663,147 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9663147T pr_change=Q130@|Nsf2-PA reported_pr_change=Q130@ (Golby et al., 2001) point mutation Nsf2[11] 3R:9,662,027..9,662,027 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T9662027R pr_change=N291K|Nsf2-PA reported_pr_change=N291K (Golby et al., 2001) point mutation Nsf2[15] 3R:9,662,020..9,662,020 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9662020T pr_change=Q294@|Nsf2-PA reported_pr_change=Q294@ (Golby et al., 2001) point mutation Nsf2[42] 3R:9,661,509..9,661,509 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9661509T pr_change=A464V|Nsf2-PA reported_pr_change=A464V (Golby et al., 2001) point mutation Nsf2[A6] 3R:9,660,871..9,660,871 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9660871T pr_change=A597V|Nsf2-PA reported_pr_change=A597V (Parks, 2004.5.6) sequence variant Nsf2[103] 3R:9,661,254..9,661,586 comment=334 nucleotide deletion, including an intron, that fuses the first two bases of codon 438 to the first base of codon 470. The result is a severely truncated protein with 37 novel amino acids following threonine 438. evidence=experimental (Golby et al., 2001)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 9355
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage  embryonic/larval midgut (Boulianne and Trimble, 1995)  larval stage  dorsal mesothoracic disc (Boulianne and Trimble, 1995)  embryonic stage | stage 5,6   (Boulianne and Trimble, 1995)  embryonic stage | stage 8-10  posterior midgut primordium (Boulianne and Trimble, 1995)  embryonic stage  embryonic/larval hindgut (Boulianne and Trimble, 1995)  embryonic stage | stage 8-11  mesoderm (Boulianne and Trimble, 1995)  embryonic stage | stage 8-11  ectoderm (Boulianne and Trimble, 1995)  embryonic stage | stage 8-10  anterior midgut primordium (Boulianne and Trimble, 1995)  larval stage  eye-antennal disc (Boulianne and Trimble, 1995)  larval stage  embryonic/larval salivary gland | restricted (Boulianne and Trimble, 1995)  embryonic stage,larval stage,adult stage   (Pallanck et al., 1995)  larval stage  imaginal disc (Boulianne and Trimble, 1995)  larval stage  ring gland (Boulianne and Trimble, 1995)  embryonic stage | stage >=14  embryonic central nervous system (Boulianne and Trimble, 1995)  larval stage  ventral thoracic disc (Boulianne and Trimble, 1995)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013998 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0013998 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0013998
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Nsf2A6 lethal | germ-line clone | larval stage | recessive Nsf255 lethal | larval stage | recessive Nsf2102 Nsf2103 Nsf211 Nsf215 Nsf221 Nsf255 lethal | pharate adult stage | recessive Nsf242 Other Phenotypes Allele wild-type Nsf2hs.PG wild-type, with Scer\GAL4bbg-C96 Nsf2WT.Scer\UAS visible Nsf2A6 visible, with Scer\GAL4bbg-C96 Nsf2EQ.Scer\UAS neuroanatomy defective, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS Phenotype manifest in Allele eye Nsf2A6 wing margin, with Scer\GAL4bbg-C96 Nsf2EQ.Scer\UAS larval neuromuscular junction, with Scer\GAL4BG487 Nsf2EQ.Scer\UAS larval neuromuscular junction, with Scer\GAL4elav.PLu Nsf2EQ.Scer\UAS
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Nsf2 Class Mutagen Stocks Known lesion Nsf2102 ethyl methanesulfonate 0 Yes Nsf2103 loss of function ethyl methanesulfonate 0 Yes Nsf211 ethyl methanesulfonate 0 Yes Nsf215 loss of function ethyl methanesulfonate 0 Yes Nsf221 hypomorph ethyl methanesulfonate 0 -- Nsf242 ethyl methanesulfonate 0 Yes Nsf255 loss of function ethyl methanesulfonate 0 Yes Nsf266 ethyl methanesulfonate 0 -- Nsf2A15 ethyl methanesulfonate 1 Yes Nsf2A6 ethyl methanesulfonate 1 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nsf2 Class Mutagen Stocks Known lesion Nsf2EQ.Scer\UAS in vitro construct | regulatory fusion in vitro construct | amino acid replacement 0 Yes Nsf2GD1327 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Nsf2Scer\UAS.cGa in vitro construct | regulatory fusion 0 Yes Nsf2WT.Scer\UAS in vitro construct | regulatory fusion 0 Yes Nsf2hs.PG in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)urd
Disrupted in	Df(3R)urd (Golby et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1327} NA P{UAS-Nsf2.EQ} NA P{UAS-Nsf2.G} NA P{UAS-Nsf2.WT} NA heat-shock construct P{hsp70-Nsf2.G} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Evidence of multiple, highly related isoforms of NSF in a single species suggests that in some tissue membranes fusion and/or secretion has a specific role during development. (Boulianne and Trimble, 1995) Drosophila has a second N-ethylmaleimide-sensitive fusion protein (NSF) homolog, Nsf2. comt and Nsf2 display overlapping but different temporal expression patterns. Multiple transcripts derived from Nsf2. (Pallanck et al., 1995)
Gene Ontology: Function, Process & Cellular Component ( 8 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR003338, InterPro:IPR003959, InterPro:IPR003960, InterPro:IPR004201 (FlyBase Curators et al., 2004-)
nucleoside-triphosphatase activity inferred from electronic annotation with InterPro:IPR003593 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ER to Golgi vesicle-mediated transport inferred from sequence or structural similarity with UniProtKB:P18708 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
intra-Golgi vesicle-mediated transport non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
neurotransmitter secretion non-traceable author statement (Lloyd et al., 2000)
synaptic vesicle priming non-traceable author statement (Lloyd et al., 2000)
vesicle-mediated transport non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Cellular Component
CV term Evidence References
cytoplasm inferred from sequence or structural similarity with UniProtKB:P18708 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Littleton et al., 1999) non-traceable author statement (Swiss-Prot Project Members, 2001.8.20)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nsf2 allele Gene References Nsf2103 comt (Golby et al., 2001) Nsf215 comt (Golby et al., 2001) Nsf255 comt (Golby et al., 2001) Nsf2EQ.Scer\UAS Syx16 (Xu et al., 2002) wg (Stewart et al., 2001) Syx5 (Xu et al., 2002) Nsf2Scer\UAS.cGa comt (Golby et al., 2001) unspecified Rbf (Laviolette et al., 2005) CG14628 (Laviolette et al., 2005) CG10801 (Laviolette et al., 2005) CG3603 (Laviolette et al., 2005) CG4119 (Laviolette et al., 2005) wuho (Laviolette et al., 2005) fz4 (Laviolette et al., 2005) Moe (Laviolette et al., 2005) ld14 (Laviolette et al., 2005) nej (Laviolette et al., 2005) CG30217 (Laviolette et al., 2005) CG1806 (Laviolette et al., 2005) CG32650 (Laviolette et al., 2005) ben (Laviolette et al., 2005) NetB (Laviolette et al., 2005) CG14411 (Laviolette et al., 2005) Fim (Laviolette et al., 2005) CG7095 (Laviolette et al., 2005) CG8798 (Laviolette et al., 2005) Baldspot (Laviolette et al., 2005) Ufd1-like (Laviolette et al., 2005) CdsA (Laviolette et al., 2005) CG7492 (Laviolette et al., 2005) LanA (Laviolette et al., 2005) CG9894 (Laviolette et al., 2005) Rab5 (Laviolette et al., 2005) RhoBTB (Laviolette et al., 2005) Psn (Mahoney et al., 2006) CG10585 (Laviolette et al., 2005) CG14651 (Laviolette et al., 2005) Gdi (Laviolette et al., 2005) CG12264 (Laviolette et al., 2005) CG6388 (Laviolette et al., 2005) Gli (Laviolette et al., 2005) brat (Laviolette et al., 2005) Tcp-1η (Laviolette et al., 2005) E2f (Laviolette et al., 2005) btn (Laviolette et al., 2005) CG11897 (Laviolette et al., 2005) phtf (Laviolette et al., 2005) ubl (Laviolette et al., 2005) Syn2 (Laviolette et al., 2005) igl (Laviolette et al., 2005) CG1888 (Laviolette et al., 2005) Cyp6v1 (Laviolette et al., 2005) sop (Laviolette et al., 2005) polo (Laviolette et al., 2005) grp (Laviolette et al., 2005) cpo (Laviolette et al., 2005) Act5C (Laviolette et al., 2005) lola (Laviolette et al., 2005) spi (Laviolette et al., 2005) ovo (Laviolette et al., 2005) GS8172 (Laviolette et al., 2005) GS32 (Laviolette et al., 2005) GS1027 (Laviolette et al., 2005) GS2192 (Laviolette et al., 2005) GS2285 (Laviolette et al., 2005) GS3087 (Laviolette et al., 2005) GS5036 (Laviolette et al., 2005) GS5071 (Laviolette et al., 2005) GS11410 (Laviolette et al., 2005) GS11471 (Laviolette et al., 2005) GS14505 (Laviolette et al., 2005) l(2)37Cg (Laviolette et al., 2005) Ubi-p5E (Laviolette et al., 2005) Snap (Laviolette et al., 2005, Babcock et al., 2004) PQBP-1 (Laviolette et al., 2005) C3G (Laviolette et al., 2005)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 66772 Drosophila PIMRider - The Drosophila Protein Interaction map • 521155
Orthologs
Genome-wide drosophilid orthologs	Dsec\GM25905 Dsim\GD20473 Dyak\GE24413
Curated drosophilid orthologs	Dpse\GA17281
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000012741 Anopheles gambiae (Ensembl) ENSBTAP00000011107 Bos taurus (Ensembl) ENSCAFP00000019878 Canis familiaris (Ensembl) ENSCINP00000014228 Ciona intestinalis (Ensembl) ENSGALP00000001579 Gallus gallus (Ensembl) ENSP00000225282 Homo sapiens (Ensembl) ENSMMUP00000007332 Macaca mulatta (Ensembl) ENSMODP00000013816 Monodelphis domestica (Ensembl) ENSPTRP00000015850 Pan troglodytes (Ensembl) ENSRNOP00000006361 Rattus norvegicus (Ensembl) GSTENP00011477001 Tetraodon nigroviridis (Ensembl) ENSXETP00000031197 Xenopus tropicalis (Ensembl) AT4G04910.1 Arabidopsis thaliana (Arabidopsis) WBGene00003818 Caenorhabditis elegans (Wormbase) DDB0185052 Dictyostelium discoideum (Dictybase) MGI:104560 Mus musculus (Informatics) SPAC1834.11c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v7743 w1118; P{GD1327}v7743
Bloomington	8282 w1118; Nsf2A15/TM6B, Tb1 8281 w1118; Nsf2A6/TM3, Sb1 Ser1
Genomic Clones ( 2 )
	BACR25B05 BACR30G22
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD09622
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT28554 LD11372 HL04960 RE17633 RH59117 RH33585 LD15205 LD31558 RE06747 AT13474 HL05916 RH27115 RE18385 GM15043 LD19337 GH09211 GM26786 RE18950 RE27527 RE17645 RE61865 RE03575 GM32081
Other clones	EK154262 EN03834 EK188053 EK040110 bs40g03 EK129016
RNAi & Array Information