Gene Dmel\Eip93F

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General Information

Symbol

Dmel\Eip93F

Species

D. melanogaster

Name

Eip93F

Annotation symbol

CG18389

Feature type

protein_coding_gene

FlyBase ID

FBgn0013948

Created / Updated

2003-12-01/2007-03-20

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

93F14-93F14

Sequence location

3R:17,786,486..17,837,910 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Eip93F (CG18389, FBgn0013948). It has the cytological map location 93F14. Its sequence location is 3R:17786486..17837910. Its molecular function is described as: transcription factor activity; DNA binding. It is involved in the biological processes: autophagic cell death; salivary gland cell autophagic cell death; induction of apoptosis by hormones; ecdysone-mediated induction of salivary gland cell autophagic cell death; larval midgut histolysis; autophagy; phagocytosis, engulfment; positive regulation of transcription, DNA-dependent; regulation of transcription. 11 alleles are reported. The phenotypes of these alleles are annotated with: embryo; dorsal mesothoracic disc; pupa; larval salivary gland; salivary gland. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93F14-93F14

Limits computationally determined from genome sequence between P{PZ}tsl⁰⁰⁶¹⁷ and P{lacW}how^j5B5&P{EP}EP738

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93F7-93F14

Located by the range spanned by the 135kb chromosome walk known to include the Eip93F transcription unit.

(Baehrecke and Thummel, 1995)

93F-93F

(determined by in situ hybridisation)

(Woodard et al., 1994)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Eip93F for information on other features

Comments on Gene Model

Translation start as per accession U25686;

C-terminus does not agree with accession U25686 or SWP:Q24079; mismatch begins in center of long ORF

EST data suggest additional 5' exon(s)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Eip93F-RA

FBtr0084169

NM_143745

9586

1165

Eip93F-RB

FBtr0112874

NM_001104395

3933

1188

Additional Transcript Data & Comments

Reported size (kB)

9.5 (northern blot)

(Baehrecke and Thummel, 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Eip93F-PA

FBpp0083567

124.0

1165

7.15

NP_652002

AAF55940

Eip93F-PB

FBpp0111787

126.8

1188

7.41

NP_001097865

ABW08726

Additional Polypeptide Data & Comments

Reported size (kD)

1221 (aa); 146 (kD)

(Baehrecke and Thummel, 1995)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0013948

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Helix-turn-helix, Psq (IPR007889)

Helix-turn-helix, Psq-like (IPR011526)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

Eip93F[1]
3R:17,831,712..17,831,712

evidence=experimental
na_change=T17831712A
pr_change=L995@|Eip93F-PA
reported_na_change= T3374A
reported_pr_change=L994@

(Lee et al., 2000)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10273

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

prepupal stage | early

pupal/adult digestive system

(Baehrecke and Thummel, 1995)

prepupal stage | late

imaginal disc

(Baehrecke and Thummel, 1995)

prepupal stage | late

pupal/adult digestive system

(Baehrecke and Thummel, 1995)

prepupal stage | late

embryonic/larval salivary gland

(Baehrecke and Thummel, 1995)

prepupal stage | early

embryonic/larval fat body

(Baehrecke and Thummel, 1995)

pupal stage | 0-40 hr PPF

(Baehrecke and Thummel, 1995)

prepupal stage | 12 hr

embryonic/larval salivary gland

(Baehrecke and Thummel, 1995)

prepupal stage | late

larval central nervous system

(Baehrecke and Thummel, 1995)

pupal stage | 196 hr

(Baehrecke and Thummel, 1995)

prepupal stage | late

embryonic/larval fat body

(Baehrecke and Thummel, 1995)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013948

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0013948

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0013948

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Eip93F^f08111

lethal | pair rule, with Scer\GAL4^h-1J3

Eip93F^Scer\UAS.cLa

lethal | pupal stage, with Scer\GAL4^vg.PM

Eip93F^Scer\UAS.cLa

lethal, with Scer\GAL4^hs.PB

Eip93F^Scer\UAS.cLa

semi-lethal | pupal stage

Eip93F¹

semi-lethal | pupal stage (with Df(3R)93F^x2)

Eip93F¹

semi-lethal | pupal stage (with Eip93F²)

Eip93F¹

semi-lethal | pupal stage (with Eip93F¹)

semi-lethal | pupal stage (with Eip93F³)

Eip93F¹

Other Phenotypes

Allele

cell death increase | pair rule, with Scer\GAL4^h-1J3

Eip93F^Scer\UAS.cLa

cell death increase, with Scer\GAL4^hs.PB

Eip93F^Scer\UAS.cLa

cell death increase, with Scer\GAL4^vg.PM

Eip93F^Scer\UAS.cLa

cell death decrease

Sterility

Allele

fertile

Eip93F^f08111

Phenotype manifest in

Allele

pupa (with Eip93F²)

Eip93F¹

pupa (with Eip93F³)

Eip93F¹

salivary gland

pupa

Eip93F¹

larval midgut & prepupa (with Df(3R)93F^x2)

Eip93F¹

pupa (with Df(3R)93F^x2)

Eip93F¹

larval salivary gland (with Df(3R)93F^x2)

Eip93F¹

pupa (with Eip93F¹)

larval salivary gland

embryo, with Scer\GAL4^h-1J3

Eip93F^Scer\UAS.cLa

embryo, with Scer\GAL4^hs.PB

Eip93F^Scer\UAS.cLa

dorsal mesothoracic disc, with Scer\GAL4^vg.PM

Eip93F^Scer\UAS.cLa

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of Eip93F	Class	Mutagen	Stocks	Known lesion
Eip93F¹	loss of function	ethyl methanesulfonate	0	--
Eip93F²	hypomorph	ethyl methanesulfonate	0	--
Eip93F³	hypomorph		0	--
Eip93F^c07152		piggyBac transposase	1	--
Eip93F^f01603		piggyBac transposase	0	--
Eip93F^f01771		piggyBac transposase	1	--
Eip93F^f08110		piggyBac transposase	0	--
Eip93F^f08111		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Eip93F	Mutagen	Stocks	Known lesion
Eip93F^GD4449	in vitro construct \| RNAi in vitro construct \| regulatory fusion	5	Yes
Eip93F^Scer\UAS.cLa	in vitro construct \| regulatory fusion	0	Yes
Eip93F^dsRNA.cKa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)e-GC3

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)93F^x2

(Lee and Baehrecke, 2001)

Df(3R)Exel6188

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4449}

P{UAS-Eip93F.L}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}Eip93F^c07152

PBac{WH}Eip93F^f01603

PBac{WH}Eip93F^f01771

PBac{WH}Eip93F^f08110

PBac{WH}Eip93F^f08111

Related Comments

Please look at the allele reports for the complete phenotype data

Ecdysone regulated puff gene.

(Woodard et al., 1994)

The Eip93F gene displays stage- and tissue-specific regulation by 20-hydroxyecdysone.

(Baehrecke and Thummel, 1995)

Gene Ontology: Function, Process & Cellular Component ( 13 )

Molecular Function

CV term

Evidence

References

DNA binding

non-traceable author statement

(Lehmann, 2002.7.1)

inferred from electronic annotation with InterPro:IPR007889, InterPro:IPR011526

(FlyBase Curators et al., 2004-)

transcription factor activity

non-traceable author statement

(Lafont, 2000)

Biological Process

CV term

Evidence

References

autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

traceable author statement

(Thummel, 2001)

autophagy

inferred from mutant phenotype

(Lee and Baehrecke, 2001)

ecdysone-mediated induction of salivary gland cell autophagic cell death

traceable author statement

(Buszczak and Segraves, 2000)

induction of apoptosis by hormones

inferred from mutant phenotype

(Lee et al., 2000)

larval midgut histolysis

traceable author statement

(Buszczak and Segraves, 2000)

phagocytosis, engulfment

traceable author statement

(Thummel, 2001)

positive regulation of transcription, DNA-dependent

traceable author statement

(Thummel, 2001)

regulation of transcription

non-traceable author statement

(Lafont, 2000)

salivary gland cell autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

non-traceable author statement

(Lee and Baehrecke, 2000)

Cellular Component

CV term

Evidence

References

nucleus

traceable author statement

(Buszczak and Segraves, 2000)

non-traceable author statement

(Thummel, 2001)

inferred from electronic annotation with InterPro:IPR007889, InterPro:IPR011526

(FlyBase Curators et al., 2004-)

polytene chromosome

traceable author statement

(Buszczak and Segraves, 2000)

non-traceable author statement

(Thummel, 2001)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Eip93F allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

69393

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18059

Dere\GG11097

Dgri\GH20931

Dmoj\GI10724

Dper\GL23807

Dpse\GA27060

Dsec\GM26391

Dsim\GD20912

Dvir\GJ23138

Dwil\GK14236

Dyak\GE10258

Curated drosophilid orthologs

Dpse\GA14915

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000029172
Anopheles gambiae (Ensembl)
GSTENP00008049001
Tetraodon nigroviridis (Ensembl)
MGI:2443930
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 9 )

Harvard

PBac{PB}Eip93F^c07152

PBac{WH}Eip93F^f01771

PBac{WH}Eip93F^f08111

VDRC

v13075

w¹¹¹⁸ P{GD4449}v13075

v45857

w¹¹¹⁸; P{GD4449}v45857

v45858

w¹¹¹⁸; P{GD4449}v45858

v45855

w¹¹¹⁸; P{GD4449}v45855/CyO

v45856

w¹¹¹⁸; P{GD4449}v45856/TM3

Bloomington

19132

w¹¹¹⁸/FM7c; PBac{WH}Eip93F^f08111

Genomic Clones ( 2 )

cDNA Clones ( 23 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0013948

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0013948

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Eip93F CG18389

(Misra, 2000.4.6)

Source for merge of: Eip93F anon-WO02059370.58 Source for merge of Eip93F anon-WO02059370.58 was sequence comparison (date:051113).

(FlyBase, 1996-)

Additional comments

Comments About Role

Eip93F may be a critical regulator of spatial and temporal patterns of steroid-triggered programmed cell death during metamorphosis.

(Lee et al., 2000)

Eip93F is required for salivary gland, autophagic, cell death. Eip93F is sufficient to trigger cellular changes that result in engulfment by phagocytes, and that coordination of the complete apoptotic response requires the rpr/grim/W cluster.

(Lee and Baehrecke, 2001)

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•