HomeGene Dmau\mariner\T
| General Information | ||||||||
|---|---|---|---|---|---|---|---|---|
| Symbol | Dmau\mariner\T | Species | D. mauritiana | |||||
| Name | mariner-element transposase | Annotation symbol | ||||||
| Feature type | transposable_element_gene | FlyBase ID | FBgn0013835 | |||||
| Created / Updated | 2006-08-20/2006-08-20 | |||||||
| Genomic Location | ||||||||
| Chromosome (arm) | Recombination map | |||||||
| Cytogenetic map | Sequence location | |||||||
Summary Information
| ||||||||
|
Automatically generated summary
See sections below for more information | The gene mariner-element transposase is referred to in FlyBase by the symbol Dmau\mariner\T (FBgn0013835). It has not been localized to the genome sequence. Its molecular function is described as: magnesium ion binding; protein homodimerization activity. It is involved in the biological process transposition, DNA-mediated. 45 alleles are reported. No phenotypic data is available. It has no annotated transcripts. | |||||||
|
Detailed Mapping Data
| ||||||||
| FlyBase Computed Cytological Location | ||||||||
Cytogenetic map Evidence for location | ||||||||
| Experimentally Determined Cytological Location | ||||||||
Cytogenetic map Notes References | ||||||||
| Experimentally Determined Recombination Data | ||||||||
| Location | ||||||||
| Left of (cM) | ||||||||
| Right of (cM) | ||||||||
| Notes | ||||||||
| Molecular Map Data | ||||||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References | ||||||||
|
Gene Model & Products
| ||||||||
| Comments on Gene Model | ||||||||
|
Transcript Data
| ||||||||
| Annotated Transcripts | ||||||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||||||
| Additional Transcript Data & Comments | ||||||||
| Reported size (kB) | ||||||||
| Comments | ||||||||
| External Data | ||||||||
| Crossreferences | ||||||||
|
Polypeptide Data
| ||||||||
| Annotated Polypeptides | ||||||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
| ||||||||
| Additional Polypeptide Data & Comments | ||||||||
| Reported size (kD) | ||||||||
| Comments | ||||||||
| External Data | ||||||||
| Linkouts | ||||||||
| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Transposase, type 1 (IPR001888)
| |||||||
|
Sequences Consistent with the Gene Model
| ||||||||
| DDBJ
/
EMBL / GenBank | DNA sequence Protein sequence Name | |||||||
| UniProtKB/Swiss-Prot | ||||||||
| UniProtKB/TrEMBL | ||||||||
| Maps to | ||||||||
| Does NOT map to | ||||||||
| Identified with | ||||||||
|
Mapped Features & Mutations
| ||||||||
Type Symbol & Location Additional Notes References | ||||||||
|
External Data
| ||||||||
| Linkouts | ||||||||
| Crossreferences | ||||||||
|
Expression Data
| ||||||||
| FlyBase-Curated Data | ||||||||
|
Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||||||
|
Summary of Transcript Expression
| ||||||||
Stage Tissue/Position
Reference
| ||||||||
| Marker for | ||||||||
| Subcellular Localization | ||||||||
| CV Term | ||||||||
|
Summary of Polypeptide Expression
| ||||||||
Stage Tissue/Position
Reference
| ||||||||
| Marker for | ||||||||
| Subcellular Localization | ||||||||
| CV Term | ||||||||
|
External Data & Images
| ||||||||
| Linkouts | ||||||||
|
Alleles & Phenotypes
| ||||||||
|
Summary of Allele Phenotypes
| ||||||||
Other Phenotypes Allele Phenotype manifest in Allele | ||||||||
|
Classical Alleles
( 0 ) | ||||||||
| For All Classical Alleles Show | ||||||||
| ||||||||
|
Alleles Carried on Transgenic Constructs
( 45 ) | ||||||||
| For All Alleles Carried on Transgenic Constructs Show | ||||||||
|
Aneuploid Aberrations
| ||||||||
|
Transgenic Constructs & Insertions
| ||||||||
| Transgenic Constructs | ||||||||
Type of construct Name Expression data | ||||||||
| Insertions | ||||||||
Type of insertions Name Expression data | ||||||||
|
Related Comments
| ||||||||
Please look at the allele reports for the complete phenotype data
PCR amplification provided regions of presumed transposase genes from D.mauritiana and 10 other insects representing 6 additional orders. Sequence analysis revealed a diverse array of mariner elements, with multiple subfamilies, indicating vertical inheritance and horizontal transfer. Genetic studies of the Mos1 transposase suggest two distinct types of regulatory mechanism: overproduction inhibition, OPI (in which excessive quantities of the wild type transposase significantly reduce overall frequency of excision of a target Dmau\mariner element) and dominant negative complementation, DNC (which is observed in a significant proportion of hypomorphic mutations in the transposase. Genetic and biochemical studies have identified a point mutation in the transposase that shows significant dominant-negative complementation when heterozygous with a wild type coding sequence; this finding suggests that the transposase functions as an oligomer. Application of the yeast two hybrid system implies the transposase consists of subunits that interact. Elevated expression of transposase above a certain threshold decreases the frequency of excision of a mariner target element. Therefore overproduction of transposase may serve as one mechanism by which transposition is regulated. The Dmau\wpch mosaicism screen efficiently reveals mutations in the Dmau\mariner\T that are defective in the excision reaction of transposition. Site-directed and EMS induced transposase mutations demonstrate that the D,D(34)D domain cannot be replaced with D,D(34)E domain, identify various essential residues in the transposase and indicate that the majority of hypomorphic transposase mutations impair the activity of the wild-type transposase. | ||||||||
|
Gene Ontology: Function, Process & Cellular Component
( 3 ) | ||||||||
|
Molecular Function
| ||||||||
CV term References | ||||||||
inferred from direct assay | ||||||||
inferred from direct assay | ||||||||
|
Biological Process
| ||||||||
CV term References | ||||||||
inferred from direct assay | ||||||||
|
Cellular Component
| ||||||||
CV term References | ||||||||
|
Sequence Ontology: Class of Gene
| ||||||||
|
Interactions & Pathways
| ||||||||
|
Summary of Genetic Interactions
| ||||||||
| Interacts with |
Please look at the allele data for full details of the genetic
interactions
Dmau\mariner\T
allele
Gene References | |||||||
|
External Data
| ||||||||
| Linkouts | ||||||||
|
Orthologs
| ||||||||
| Genome-wide drosophilid orthologs | ||||||||
| Curated drosophilid orthologs | ||||||||
| Linkouts | ||||||||
|
Functional Complementation between Species
| ||||||||
|
Inter-Species Misexpression Data
| ||||||||
| Produces phenotype in | ||||||||
| Produces NO phenotype in | ||||||||
|
Stocks & Reagents
| ||||||||
|
Stocks Listed in FlyBase
( 0 ) | ||||||||
|
Genomic Clones
( 0 ) | ||||||||
|
cDNA Clones
( 0 ) | ||||||||
| cDNA Clones, Fully Sequenced | ||||||||
| BDGP DGC clones | ||||||||
| Other clones | ||||||||
| cDNA Clones, End Sequenced (ESTs) | ||||||||
| BDGP DGC clones | ||||||||
| Other clones | ||||||||
|
RNAi & Array Information
| ||||||||
| Affy Oligo | ||||||||
| Linkouts | ||||||||
|
Antibody Information
| ||||||||
|
Other Information
| ||||||||
|
Discoverer
| ||||||||
|
Etymology
| ||||||||
|
Identification
| ||||||||
|
Position Effect Variegation Data
| ||||||||
|
Relationship to Other Genes
| ||||||||
| Source for database identity of | ||||||||
| Source for database merge of | ||||||||
| Encoded by | ||||||||
| Additional comments | ||||||||
|
Comments About Role
| ||||||||
Dmau\mariner transposase seems to distinguish between the 5' and 3' ends of the Dmau\mariner element. Mutations in the 5' inverted repeat are smaller and more frequent than in the 3' terminal repeat, but secondary mutations in elements with a previous 5' lesion tend to have 3' lesions resembling those normally found at the 5' end. | ||||||||
|
Comments About Molecular Function
| ||||||||
The ITR binding domain of Dmau\mariner\T has been localised between amino acids 1 and 141. Dmau\mariner\T encodes a transposase which excises Dmau\mariner\T from plasmid DNA in vitro. The transposase recognises the inverted repeats at the end of Dmau\mariner\T in a sequence specific manner via a DNA binding domain located within the N-terminal 120 amino acids. It binds the right hand inverted repeat about five times more strongly than the left. Dmau\mariner\T transposase monomers bind to each other. Mutations along the length of the Dmau\mariner\T transposase protein reduce the monomer interaction, indicating that no single protein-protein interaction domain is involved. During Dmau\mariner transposon DNA cleavage by Dmau\mariner\T: the nontransferred strand is cleaved initially; first strand cleavage is not tightly coupled to second strand cleavage and can occur independently of synapsis; and second strand cleavage of mariner does not occur via a hairpin intermediate. | ||||||||
|
Other Comments
| ||||||||
|
External Crossreferences & Linkouts
| ||||||||
| Sequence Crossreferences | ||||||||
| Other Crossreferences | ||||||||
InterPro
domains - A database of protein families, domains, and functional sites
• Transposase, type 1 (IPR001888)
| ||||||||
| Linkouts | ||||||||
|
Synonyms & Secondary IDs
( 6 ) | ||||||||
| Reported As | ||||||||
| Symbol Synonym | Dmau\mariner\T mariner Mos1 Mos1 Transposase unnamed | |||||||
| Name Synonym | mariner-element transposase | |||||||
| Secondary FlyBase IDs | ||||||||
|
References
( 43 ) | ||||||||
| Generate a list of | ||||||||
| List References by type |
| |||||||
|
Recent research papers ( 1 ) | ||||||||
| ||||||||
|
Recent reviews (0)
| ||||||||
| All reviews listed in FlyBase were published before 2006 | ||||||||