A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Cdk5

General Information
SymbolDmel\Cdk5SpeciesD. melanogaster
NameCyclin-dependent kinase 5Annotation symbolCG8203
Feature typeprotein_coding_geneFlyBase IDFBgn0013762
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map2-
Cytogenetic map52A13-52A13Sequence location2R:11,457,035..11,458,918 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Cyclin-dependent kinase 5 is referred to in FlyBase by the symbol Cdk5 (CG8203, FBgn0013762). It has the cytological map location 52A13. Its sequence location is 2R:11457035..11458918. Its molecular function is described as: cyclin-dependent protein kinase activity; protein serine/threonine kinase activity; cyclin binding; ATP binding. It is involved in the biological processes: protein amino acid phosphorylation; neurotransmitter secretion; cell cycle; synaptic vesicle transport; axonogenesis. 3 alleles are reported. The phenotype of these alleles is annotated with embryonic nervous system. It has one annotated transcript and one annotated polypeptide.
External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
52A13-52A13  
Limits computationally determined from genome sequence between P{lacW}Vha36k07207 and P{lacW}l(2)k05713k05713  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
52A-52A
 
(Lehner, 1996.7.23)
52A14-52C9
(determined by in situ hybridisation)  
(Hellmich et al., 1994)
Experimentally Determined Recombination Data
Location
2-
(Lehner, 1996.7.23)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Cdk5 for information on other features
detailed view FBtr0087352 FBtr0087351 FBtr0087350 FBtr0087339 FBtr0087349 FBpp0086484 FBpp0086485 FBpp0086483 FBpp0086472 FBpp0086482 FBti0047752 FBti0024557
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Cdk5-RA
FBtr0087350
 NM_057732
  1598
  294
Additional Transcript Data & Comments
Reported size (kB)
1.6 (northern blot)
(Sauer et al., 1996)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Cdk5-PA  
FBpp0086483  
33.2  
294  
7.82  
  NP_477080
  AAF58119
Additional Polypeptide Data & Comments
Reported size (kD)
294 (aa)
(Sauer et al., 1996)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Protein kinase, core (IPR000719)
Serine/threonine protein kinase (IPR002290)
Serine/threonine protein kinase, active site (IPR008271)
Protein kinase-like (IPR011009)
Protein kinase ATP binding, conserved site (IPR017441)
Serine/threonine protein kinase-related (IPR017442)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA816484
 
AC006242
 
AC008369
 
AE003810
AAF58119
 
AW941483
 
AY061049
AAL28597
 
BI608064
 
BI610200
 
BI610668
 
BI621709
 
BI623830
 
BI631040
 
BI640637
 
BP559231
 
BP559232
 
EC052536
 
EC059709
 
EC066982
 
EC078464
 
EC238869
 
EC259454
 
EL874934
 
EL874935
 
U21552
AAA63754
 
X99511
CAA67861
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage-adult stage
     
    (Sauer et al., 1996)
     embryonic stage
     embryonic central nervous system
    (Sauer et al., 1996)
     embryonic stage | early
     
    (Sauer et al., 1996)
     embryonic stage
     embryonic nervous system
    (Sauer et al., 1996)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage
       
      (Sauer et al., 1996)
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Phenotype manifest in
        Allele
        embryonic nervous system, with Scer\GAL4unspecified
        hide Classical Alleles ( 0 )
        For All Classical Alleles Show

        Allele of Cdk5ClassMutagenStocksKnown lesion
        hide Alleles Carried on Transgenic Constructs ( 3 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Cdk5ClassMutagenStocksKnown lesion
        Cdk5GD138402 Yes
        Cdk5K33A.Scer\UAS.T:Zzzz\FLAG0 Yes
        Cdk5Scer\UAS.T:Zzzz\FLAG0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Disrupted in
        (Ryder, 2004.4.26)
        (Sauer et al., 1996)
        (Sauer et al., 1996)
        (Sauer et al., 1996)
        (Sauer et al., 1996)
        Not disrupted in
        (Sauer et al., 1996)
        (Sauer et al., 1996)
        (Sauer et al., 1996)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD13840}
        NA
        P{UAS-Cdk5-FLAG}
        NA
        P{UAS-Cdk5K33A-FLAG}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Cdk5 has been cloned and sequenced.
        (Hellmich et al., 1994)
        RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.
        (Kiger et al., 2003)
        hide Gene Ontology: Function, Process & Cellular Component ( 10 )
        hide Molecular Function
        CV term
        Evidence
        References
        ATP binding
        inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441, InterPro:IPR017442
        (FlyBase Curators et al., 2004-)
        cyclin binding
        non-traceable author statement
        (Swiss-Prot Project Members, 1996.2.1)
        cyclin-dependent protein kinase activity
        inferred from sequence or structural similarity
        (Hellmich et al., 1994)
        inferred from sequence or structural similarity with SGD_LOCUS:CDC28; SGD:S0000364
        (FlyBase, 1992-)
        inferred from direct assay
        (Connell-Crowley et al., 2000)
        protein serine/threonine kinase activity
        non-traceable author statement
        (Lehner, 1996.7.23)
        non-traceable author statement
        (Morrison et al., 2000)
        non-traceable author statement
        (Swiss-Prot Project Members, 1996.2.1)
        hide Biological Process
        CV term
        Evidence
        References
        axonogenesis
        inferred from mutant phenotype inferred from genetic interaction with FLYBASE:α; FB:FBgn0027491
        (Connell-Crowley et al., 2000)
        cell cycle
        inferred from mutant phenotype
        (Kiger et al., 2003)
        neurotransmitter secretion
        non-traceable author statement
        (Bellen, 2000.12.25)
        protein amino acid phosphorylation
        inferred from sequence or structural similarity
        (Hellmich et al., 1994)
        non-traceable author statement
        (Morrison et al., 2000)
        inferred from direct assay
        (Connell-Crowley et al., 2000)
        inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271, InterPro:IPR017441, InterPro:IPR017442
        (FlyBase Curators et al., 2004-)
        synaptic vesicle transport
        inferred from electronic annotation
        (Roth and Foulger, 2002.4.24)
        hide Cellular Component
        CV term
        Evidence
        References
        cyclin-dependent protein kinase 5 activator complex
        inferred from physical interaction with FLYBASE:α; FB:FBgn0027491
        (Connell-Crowley et al., 2000)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Cdk5 allele
        Gene
        References
        Cdk5K33A.Scer\UAS.T:Zzzz\FLAG
        Cdk5α
        (Connell-Crowley et al., 2000)
        Cdk5Scer\UAS.T:Zzzz\FLAG
        Cdk5α
        (Connell-Crowley et al., 2000, Shulman and Feany, 2003)
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 2 )
        VDRC
        v35855
        w1118; P{GD13840}v35855
        v35856
        w1118; P{GD13840}v35856
        hide Genomic Clones ( 1 )
        hide cDNA Clones ( 10 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        polyclonal
        (Sauer et al., 1996)
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for identity of: Cdk5 CG8203
        (Li, 1999.11)
        Source for database merge of
        Additional comments
        hide Comments About Role
        hide Comments About Molecular Function
        Promoter structures of Flo abut to or overlap with the 3'UTR of Cdk5. Overall orientation not stated: Cdk5+ Flo+
        (Hoehne et al., 1999)
        hide Other Comments
        Isolated from a genomic library, using a rat CDK5 cDNA as a probe.
        (Hellmich et al., 1994)
        Characterisation of Cdk5.
        (Sauer et al., 1996)
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
        (Rogers et al., 2003)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/Swiss-Prot
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Protein kinase, core (IPR000719)
        Serine/threonine protein kinase (IPR002290)
        Serine/threonine protein kinase, active site (IPR008271)
        Protein kinase-like (IPR011009)
        Protein kinase ATP binding, conserved site (IPR017441)
        Serine/threonine protein kinase-related (IPR017442)
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DEDB - Drosophila exon database: splicing graphs
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        InParanoid orthologs - Eukaryotic orthologs
        •
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        •
        PANTHER - Protein classification by function, families, and pathways
        •
        hide Synonyms & Secondary IDs ( 6 )
        Reported As
        Symbol Synonym
        CDK5
        (Shulman and Feany, 2003, Rascle et al., 2003, Bellen, 2000.12.25, Hellmich, 1995.2.23)
        cdk5
        (Lehner, 1996.7.23, Hoehne et al., 1999, Sauer et al., 1996)
        Cdk5
        (Kissler and Suter, 2007, Connell-Crowley et al., 2000)
        CG8203
        (Morrison et al., 2000, Verstreken and Bellen, 2000.9.4, Rogers et al., 2003)
        DmCdk5
        (Kolonin and Finley, 1998)
        Name Synonym
        Cyclin-dependent kinase 5
        (Rogers et al., 2003)
        Secondary FlyBase IDs
          hide References ( 39 )
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          hide Recent research papers ( 1 )
          Bjorklund et al., 2006, Nature 439(7079): 1009--1013
          Identification of pathways regulating cell size and cell-cycle progression by RNAi. [FBrf0191233]
          Show all research papers ...