Gene Dmel\Caki

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General Information

Symbol

Dmel\Caki

Species

D. melanogaster

Name

Calcium/calmodulin-dependent protein kinase

Annotation symbol

CG6703

Feature type

protein_coding_gene

FlyBase ID

FBgn0013759

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

93F10-93F12

Sequence location

3R:17,611,467..17,648,364 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Calcium/calmodulin-dependent protein kinase is referred to in FlyBase by the symbol Caki (CG6703, FBgn0013759). It has the cytological map location 93F10-93F12. Its sequence location is 3R:17611467..17648364. Its molecular function is described as: guanylate kinase activity; protein serine/threonine kinase activity; calmodulin-dependent protein kinase activity; ATP binding; protein binding. It is involved in the biological processes: adult walking behavior; neurotransmitter secretion; synaptic vesicle docking during exocytosis; synaptic vesicle targeting; protein amino acid phosphorylation; cell adhesion; regulation of cell shape; establishment and/or maintenance of cell polarity. 7 alleles are reported. No phenotypic data is available. It has 5 annotated transcripts and 5 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93F10-93F12

Limits computationally determined from genome sequence between P{PZ}tsl⁰⁰⁶¹⁷ and P{lacW}how^j5B5&P{EP}EP738

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93F-93F

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones. Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

93F-93F

(determined by in situ hybridisation)

(Dimitratos et al., 1997)

93F-93F

(Dimitratos et al., 1997)

93E-93E

(determined by in situ hybridisation)

(Martin and Ollo, 1996)

93F6-93F14

(Martin et al., 1994)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Caki+ tsl+ anon-93Fa+ tete?

(Martin et al., 1994)

Gene Model & Products

Please see the GBrowse view of Dmel\Caki for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Caki-RA

FBtr0084162

NM_169970

4129

591

Caki-RB

FBtr0084161

NM_079717

4113

898

Caki-RC

FBtr0084163

NM_169971

1963

315

Caki-RD

FBtr0112872

NM_001104392

4032

591

Caki-RE

FBtr0112873

NM_001104393

2601

469

Additional Transcript Data & Comments

Reported size (kB)

7.0, 4.0 (northern blot)

(Martin and Ollo, 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Caki-PA

FBpp0083560

65.2

591

6.87

NP_732661

AAF55921

Caki-PB

FBpp0083559

100.9

898

6.20

NP_524441

AAF55922

Caki-PC

FBpp0083561

36.0

315

5.77

NP_732662

AAF55920

Caki-PD

FBpp0111785

65.2

591

6.87

NP_001097862

ABW08723

Caki-PE

FBpp0111786

53.3

469

6.47

NP_001097863

ABW08724

Additional Polypeptide Data & Comments

Reported size (kD)

571 (aa); 63 (kD predicted)

(Martin and Ollo, 1996)

Comments

Constitutively expressed Caki protein was shown to be enzymatically active in a cotransfection assay in rat pituitary cells. Sequence analysis indicates that Caki may be a member of a new branch of CaM kinases.

(Martin and Ollo, 1996)

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0013759

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, core (IPR000719)

Src homology-3 (IPR001452)

PDZ/DHR/GLGF (IPR001478)

Serine/threonine protein kinase (IPR002290)

L27 (IPR004172)

Guanylate kinase (IPR008144)

Guanylate kinase/L-type calcium channel region (IPR008145)

Serine/threonine protein kinase, active site (IPR008271)

Protein kinase-like (IPR011009)

Variant SH3 (IPR011511)

L27, C-terminal (IPR014775)

Protein kinase ATP binding, conserved site (IPR017441)

Serine/threonine protein kinase-related (IPR017442)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24210

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10237

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

oogenesis stage,adult stage | stage S10B

polar follicle cell

(Martin and Ollo, 1996)

embryonic stage | stage 11

neuroblast

(Martin and Ollo, 1996)

embryonic stage | stage 14

Malpighian tubule

(Martin and Ollo, 1996)

embryonic stage | stage 14

embryonic/larval hindgut

(Martin and Ollo, 1996)

embryonic stage | stage 8-12

anterior midgut primordium

(Martin and Ollo, 1996)

pupal stage

central nervous system

(Martin and Ollo, 1996)

embryonic stage | stage 8-12

posterior midgut primordium

(Martin and Ollo, 1996)

oogenesis stage,adult stage | female

ovary

(Martin and Ollo, 1996)

oogenesis stage,adult stage

nurse cell

(Martin and Ollo, 1996)

adult stage

adult head

(Martin and Ollo, 1996)

adult stage

(Martin and Ollo, 1996)

embryonic stage | stage >=9

embryonic central nervous system | presumptive | restricted

(Martin and Ollo, 1996)

embryonic stage | 8-24 hr

(Martin and Ollo, 1996)

larval stage

larval central nervous system

(Martin and Ollo, 1996)

embryonic stage

(Martin and Ollo, 1996)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

adult stage

lobula

(Martin and Ollo, 1996)

adult stage

adult brain

(Martin and Ollo, 1996)

adult stage

lobula plate

(Martin and Ollo, 1996)

adult stage

optic lobe

(Martin and Ollo, 1996)

adult stage

lamina

(Martin and Ollo, 1996)

adult stage

medulla neuropil

(Martin and Ollo, 1996)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013759

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0013759

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0013759

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Caki

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Caki

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Caki^EY07081

Other Phenotypes

Allele

courtship defective

Caki^X-307

locomotor behavior defective

Sterility

Allele

fertile

Caki^EY07081

Phenotype manifest in

Allele

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Caki	Mutagen	Stocks	Known lesion
Caki^EY07081	P-element activity	1	--
Caki^X-307	X ray	1	Yes
Caki^X-313	X ray	1	Yes
Caki^f00990	piggyBac transposase	1	--
Caki^f07134	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Caki	Class	Mutagen	Stocks	Known lesion
Caki^GD10585		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Caki^Scer\UAS.cBa		in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)e-BS2

Df(3R)e-GC3

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)Exel6187

(Ryder, 2004.4.26)

Df(3R)caki^X-313

(Martin and Ollo, 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10585}

P{UAS-Cmg.B}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}Caki^EY07081

P{XP}Caki^d00753

miscellaneous insertions

PBac{WH}Caki^f00990

PBac{WH}Caki^f07134

Related Comments

Please look at the allele reports for the complete phenotype data

Mutant flies, in which an encoding part of the gene is removed, display a substantial reduction in walking speed.

(Martin and Ollo, 1996)

Cloning and characterisation of Caki.

(Dimitratos et al., 1997)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become round and detached. Kc167 cells are unaffected.

(Kiger et al., 2003)

Gene Ontology: Function, Process & Cellular Component ( 15 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441, InterPro:IPR017442

(FlyBase Curators et al., 2004-)

calmodulin-dependent protein kinase activity

inferred from direct assay

(Martin et al., 1994)