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Gene Dmel\Mtor

General Information
Symbol	Dmel\Mtor	Species	D. melanogaster
Name	Megator	Annotation symbol	CG8274
Feature type	protein_coding_gene	FlyBase ID	FBgn0013756
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48C5-48C5	Sequence location	2R:7,740,124..7,748,364 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Megator is referred to in FlyBase by the symbol Mtor (CG8274, FBgn0013756). It has the cytological map location 48C5. Its sequence location is 2R:7740124..7748364. Its molecular function is unknown. It is involved in the biological processes: spindle assembly; protein import into nucleus. 9 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48C5-48C5   Limits computationally determined from genome sequence between P{EP}walEP825&P{PZ}wal02516 and P{PZ}Ef1α48D01275
Experimentally Determined Cytological Location
Cytogenetic map Notes References 48C3-48C3   (Zimowska, 1997.3.4) 48C5-48C6 (determined by in situ hybridisation)   (Spradling et al., 1999) 48C-48C (determined by in situ hybridisation)   (Zimowska et al., 1997) 48C-48C   (Zimowska et al., 1997) 48C5-48C6   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Mtor for information on other features
Comments on Gene Model
	DGC clone appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mtor-RA FBtr0088033  NM_057719   7435   2346
Additional Transcript Data & Comments
Reported size (kB)	8 (northern blot) (Zimowska et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Mtor-PA   FBpp0087140   262.4   2346   4.75     NP_477067   AAF58615
Additional Polypeptide Data & Comments
Reported size (kD)	2346 (aa); 260 (kD observed); 262 (kD predicted) (Zimowska et al., 1997)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0013756
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tetratricopeptide, MLP1/MLP2-like (IPR012929)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202912   AA391203   AA392380   AA538950   AA803576   AA803909   AA941885   AC005466   AE003824 AAF58615   AI297424   AI455058   AI512281   AI530778   AI531814   AQ025733   AW944176   AX093880   AY050227 AAK84926   BI141985   BI578676   BT001737 AAN71492   CK657453   CO154077   CO298040   CO339268   CZ471280   CZ471281   EC073202   EC081063   EC083438   EC221436   EC230416   EC234297   EC239388   EC241565   EC251682   EC258506   EC264609   U91980 AAC47506
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O01385 Q8IGK6 Q961T0 A1Z8P9
Maps to	DS03299 (FlyBase, 1992-)
Does NOT map to
Identified with	RE72573 SD01380 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12899
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage,larval stage,adult stage   (Zimowska et al., 1997)
Marker for
Subcellular Localization
CV Term	nucleus (Zimowska et al., 1997)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013756 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0013756 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0013756
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Mtorrv1 lethal | recessive Mtork03905 lethal | embryonic stage Mtork03905 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Mtor Class Mutagen Stocks Known lesion Mtorc07140 piggyBac transposase 1 -- Mtork03905 loss of function P-element activity 1 Yes Mtorrv1 Δ2-3 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mtor Class Mutagen Stocks Known lesion MtorCT.MtnA.T:SV5\V5 in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | deletion 0 Yes MtorFL.MtnA.T:SV5\V5 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes MtorGD13871 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes MtorM.MtnA.T:SV5\V5 in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | deletion 0 Yes MtorNT.MtnA.T:SV5\V5 in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | deletion 0 Yes MtordsRNA.cMa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful duplication	Dp(2;2)Y3b
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13871} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Mtorc07140 NA insertion of enhancer trap P{lacW}Mtork03905 No
Related Comments
	Please look at the allele reports for the complete phenotype data Mtor gene product localises to the nuclear periphery in interphase, remains concentrated in the chromosomal region during metaphase and early anaphase, and isolates biochemically in the nuclear pore complex-lamina fraction (FBrf0048434). (Zimowska-Handler et al., 1995) Mtor has been cloned and sequenced, and its expression pattern and localisation within the cell has been determined. (Zimowska et al., 1997) Homozygous embryos die before hatching and have spindle abnormalities and chromosome segregation defects. (Rath et al., 2003) dsRNA made from templates generated with primers directed against this gene and injected into S2 cells causes a reduction in the number of cells undergoing mitosis. (Qi et al., 2004)
Gene Ontology: Function, Process & Cellular Component ( 5 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
protein import into nucleus inferred from electronic annotation with InterPro:IPR012929 (FlyBase Curators et al., 2004-)
spindle assembly inferred from direct assay (Qi et al., 2004)
Cellular Component
CV term Evidence References
nuclear pore inferred from direct assay (Zimowska et al., 1997) non-traceable author statement (Zimowska, 1997.3.4)
nucleus inferred from direct assay (Zimowska and Paddy, 2002) inferred from direct assay (Qi et al., 2004)
spindle midzone inferred from direct assay (Qi et al., 2004)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mtor allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62050 Drosophila PIMRider - The Drosophila Protein Interaction map • 521874
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13100 Dere\GG20225 Dgri\GH21847 Dmoj\GI19089 Dpse\GA25042 Dsec\GM21310 Dvir\GJ20063 Dwil\GK21555 Dyak\GE12385
Curated drosophilid orthologs
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000012828 Anopheles gambiae (Ensembl) ENSBTAP00000015848 Bos taurus (Ensembl) ENSCAFP00000020227 Canis familiaris (Ensembl) ENSCINP00000002206 Ciona intestinalis (Ensembl) NEWSINFRUP00000180152 Takifugu rubripes (Ensembl) ENSGALP00000008171 Gallus gallus (Ensembl) ENSP00000264142 Homo sapiens (Ensembl) ENSMMUP00000011914 Macaca mulatta (Ensembl) ENSMODP00000014586 Monodelphis domestica (Ensembl) ENSPTRP00000002985 Pan troglodytes (Ensembl) ENSRNOP00000003318 Rattus norvegicus (Ensembl) GSTENP00033628001 Tetraodon nigroviridis (Ensembl) ENSXETP00000037857 Xenopus tropicalis (Ensembl) AT1G79280.1 Arabidopsis thaliana (Arabidopsis) WBGene00019940 Caenorhabditis elegans (Wormbase) ZDB-GENE-030131-6410 Danio rerio (Zfin) DDB0187764 Dictyostelium discoideum (Dictybase) MGI:1922066 Mus musculus (Informatics) YKR095W Saccharomyces cerevisiae (Yeastgenome) SPCC162.08c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Harvard	- PBac{PB}Mtorc07140
VDRC	v24265 w1118; P{GD13871}v24265
Bloomington	10537 y1 w67c23; P{lacW}Mtork03905/CyO
Genomic Clones ( 1 )
	BACR26P21
cDNA Clones ( 18 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE72573 SD01380
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD15969 GM13537 LD11073 GM10796 SD03083 LD27207 LD11233 LP11721 LD18629 LD10427 LP23764 LD03328 LD44065
Other clones	EN03435 EP01354 EK171204
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0013756 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0013756
Antibody Information
	polyclonal (Zimowska et al., 1997)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: Tpr Megator (Johansen, 2003.2.18)
Source for database merge of	Source for merge of: Mtor l(2)k03905 (Qi et al., 2004)
Additional comments
Comments About Role
Comments About Molecular Function
	Mtor localises to the nuclear pore complex and interchromosomal regions during interphase, but reorganises to a spindle matrix structure colocalizing with Skeletor during prophase. (Rath et al., 2003)
Other Comments
	Area matching Drosophila Tpr homologue gene, Acc. No. U91980. (Davies, 2001.3.15) Isolated from a 0-16 hour embryo cDNA expression library, using monoclonal antibodies Mtor and Bx93 as probes. (Zimowska et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057719 RefSeq (Proteins) • NP_477067 DDBJ / EMBL / GenBank • AC005466 AE003824 AAF58615 AI530778 AQ025733 AW944176 AX093880 AY050227 AAK84926 BI578676 BT001737 AAN71492 CZ471280 CZ471281 U91980 AAC47506 UniProtKB/TrEMBL • O01385 Q8IGK6 Q961T0 A1Z8P9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tetratricopeptide, MLP1/MLP2-like (IPR012929)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62050 DEDB - Drosophila exon database: splicing graphs • 12899 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0013756 Drosophila PIMRider - The Drosophila Protein Interaction map • 521874 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013756 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0013756 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0013756 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0013756 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0013756 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000012828 Anopheles gambiae (Ensembl) ENSBTAP00000015848 Bos taurus (Ensembl) ENSCAFP00000020227 Canis familiaris (Ensembl) ENSCINP00000002206 Ciona intestinalis (Ensembl) NEWSINFRUP00000180152 Takifugu rubripes (Ensembl) ENSGALP00000008171 Gallus gallus (Ensembl) ENSP00000264142 Homo sapiens (Ensembl) ENSMMUP00000011914 Macaca mulatta (Ensembl) ENSMODP00000014586 Monodelphis domestica (Ensembl) ENSPTRP00000002985 Pan troglodytes (Ensembl) ENSRNOP00000003318 Rattus norvegicus (Ensembl) GSTENP00033628001 Tetraodon nigroviridis (Ensembl) ENSXETP00000037857 Xenopus tropicalis (Ensembl) AT1G79280.1 Arabidopsis thaliana (Arabidopsis) WBGene00019940 Caenorhabditis elegans (Wormbase) ZDB-GENE-030131-6410 Danio rerio (Zfin) DDB0187764 Dictyostelium discoideum (Dictybase) MGI:1922066 Mus musculus (Informatics) YKR095W Saccharomyces cerevisiae (Yeastgenome) SPCC162.08c Schizosaccharomyces pombe (Sanger) PANTHER - Protein classification by function, families, and pathways • FBgn0013756
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	Bx34 (Qi et al., 2004, Stathopoulos et al., 2004, Zimowska et al., 1997, Zimowska et al., 1996, Zimowska-Handler et al., 1995, Zimowska-Handler et al., 1995, Zimowska-Handler et al., 1994, Giot et al., 2003, ) CG8274   l(2)k03905 (Spradling et al., 1999, BDGP Project Members, 1994-1999, ) Mtor (Straub and Becker, 2007, Mendjan et al., 2006, ) Tpr (Paddy, 2000, Zimowska and Paddy, 2002, Zimowska and Paddy, 1999, Zimowska, 1997.3.4, Zimowska and Paddy, 1999, Lamian et al., 1998, ) TPR (Lasky, 2000)
Name Synonym	lethal (2) k03905   Megator (Johansen, 2003.2.18, Rath et al., 2003)
Secondary FlyBase IDs
	FBgn0019919 FBgn0022212
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 1 ) Review  ( 2 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 13 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Computer file  ( 4 ) Patent  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 1 )
	Mendjan et al., 2006, Molec. Cell 21(6): 811--823 Nuclear pore components are involved in the transcriptional regulation of dosage compensation in Drosophila. [FBrf0191180] Show all research papers ...
Recent reviews ( 1 )
	Straub and Becker, 2007, Nature Rev. Genet. 8(1): 47--57 Dosage compensation: the beginning and end of generalization. [FBrf0195275] Show all reviews ...