A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Bgb

General Information
SymbolDmel\BgbSpeciesD. melanogaster
NameBig brotherAnnotation symbolCG7959
Feature typeprotein_coding_geneFlyBase IDFBgn0013753
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map3-
Cytogenetic map62A9-62A9Sequence location3L:1,639,702..1,640,948 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Big brother is referred to in FlyBase by the symbol Bgb (CG7959, FBgn0013753). It has the cytological map location 62A9. Its sequence location is 3L:1639702..1640948. Its molecular function is described as: transcription coactivator activity; protein heterodimerization activity; transcription factor binding. It is involved in the biological processes: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter. 9 alleles are reported. The phenotype of these alleles is annotated with abdominal vch1 neuron. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
62A9-62A9  
Limits computationally determined from genome sequence between P{EP}rhoEP3704&P{PZ}l(3)0622606226 and P{PZ}dlt04276  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
62A10-62A10
 
(Golling, 1995.3.4)
62A10-62A10
(determined by in situ hybridisation)  
(Fujioka et al., 1996)
59F4-59F6
(determined by in situ hybridisation)  
(Golling et al., 1995)
Experimentally Determined Recombination Data
Location
3-
(Golling, 1995.3.4)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: Bgb+ Bro+
(Fujioka et al., 1996)
hide Gene Model & Products
Please see the GBrowse view of Dmel\Bgb for information on other features
detailed view FBtr0072819 FBtr0072815 FBtr0072814 FBtr0072813 FBtr0072758 FBtr0072812 FBtr0072811 FBpp0072696 FBpp0072695 FBpp0072641 FBpp0072693 FBpp0072694 FBti0021560 FBti0038886
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Bgb-RA
FBtr0072758
 NM_057717
  1247
  213
Additional Transcript Data & Comments
Reported size (kB)
0.994 (longest cDNA)
(Golling et al., 1996)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Bgb-PA  
FBpp0072641  
25.2  
213  
7.50  
  NP_477065
  AAF47533
Additional Polypeptide Data & Comments
Reported size (kD)
184 (aa)
(Golling et al., 1996)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Core binding factor, beta subunit (IPR003417)
TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles
•
  • T02222
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA263870
 
AA439134
 
AA439177
 
AC005557
 
AE003472
AAF47533
 
AI542095
 
AW944552
 
AY058784
AAL14013
 
BF499777
 
BH740810
 
BH740811
 
BI168743
 
BI369253
 
BI639175
 
BT022750
 
CO300972
 
DN153833
 
EC062497
 
EC082975
 
EC194734
 
U22177
AAB03676
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    Bgb[9]
    3L:1,640,417..1,640,417
    evidence=experimental
    na_change=A1640417C
    pr_change=E141D|Bgb-PA
    reported_na_change=A801C
    reported_pr_change=E112D
    (Kaminker et al., 2001, Kaminker, 2005.6.7)
    point mutation
    Bgb[D]
    3L:1,640,427..1,640,427
    evidence=experimental
    na_change=G1640427A
    pr_change=G145R|Bgb-PA
    reported_na_change=G808A
    reported_pr_change=G116R
    (Kaminker et al., 2001, Kaminker, 2005.6.7)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage
     
    (Golling et al., 1996)
     embryonic stage | stage >=13
     embryonic central nervous system
    (Golling et al., 1996)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Bgb
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for Bgb
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | embryonic stage | recessive
        Other Phenotypes
        Allele
        wild-type
        Phenotype manifest in
        Allele
        abdominal vch1 neuron
        abdominal vch1 neuron (with Df(3L)Bgb-K4)
        hide Classical Alleles ( 3 )
        For All Classical Alleles Show

        Allele of BgbClassMutagenStocksKnown lesion
        Bgb90 Yes
        BgbD0 Yes
        BgbKG037791 --
        hide Alleles Carried on Transgenic Constructs ( 6 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of BgbClassMutagenStocksKnown lesion
        BgbGD42620 Yes
        BgbS.Act5C0 Yes
        BgbScer\UAS.cLa0 Yes
        BgbcKa0 Yes
        BgbdsRNA.cKa0 Yes
        Bgbhs.PK0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Disrupted in
        (Kaminker et al., 2001)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD4262}
        NA
        P{UAS-Bgb.L}
        NA
        heat-shock construct
        P{hs-Bgb.K}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        P{SUPor-P}BgbKG03779
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        The Bro family of proteins interacts with run-domain proteins via the run-domain and alters their DNA binding characteristics.
        (Golling et al., 1995)
        Bgb has been cloned and sequenced, and its expression pattern has been analysed.
        (Golling et al., 1996)
        hide Gene Ontology: Function, Process & Cellular Component ( 7 )
        hide Molecular Function
        CV term
        Evidence
        References
        protein heterodimerization activity
        traceable author statement
        (Evans and Banerjee, 2003)
        transcription coactivator activity
        inferred from sequence or structural similarity with HGNC_gene:CBFB; HGNC:1539; OMIM:121360
        (FlyBase, 1992-)
        inferred from direct assay inferred from physical interaction with FLYBASE:run; FB:FBgn0003300 inferred from sequence or structural similarity with HGNC_gene:RUNX2; HGNC:10472; OMIM:600211
        (Golling et al., 1996)
        transcription factor binding
        traceable author statement
        (Evans and Banerjee, 2003)
        hide Biological Process
        CV term
        Evidence
        References
        positive regulation of transcription from RNA polymerase II promoter
        inferred from sequence or structural similarity with MGD:Cbfb; MGI:MGI:99851
        (FlyBase, 1992-)
        regulation of transcription from RNA polymerase II promoter
        traceable author statement
        (Canon and Banerjee, 2000)
        hide Cellular Component
        CV term
        Evidence
        References
        core-binding factor complex
        inferred from sequence or structural similarity with HGNC_gene:CBFB; HGNC:1539; OMIM:121360
        (FlyBase, 1992-)
        nucleus
        inferred from direct assay
        (Golling et al., 1996)
        non-traceable author statement
        (Swiss-Prot Project Members, 1997.2.1)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Bgb allele
        Gene
        References
        Bgb9
        lz
        (Kaminker et al., 2001)
        BgbD
        lz
        (Kaminker et al., 2001)
        BgbdsRNA.cKa
        Bro
        (Kaminker et al., 2001)
        BgbScer\UAS.cLa
        lz
        (Li and Gergen, 1999)
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 1 )
        Bloomington
        13509
        y1; P{SUPor-P}BgbKG03779 ry506/TM3, Sb1 Ser1
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 11 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for database merge of
        Additional comments
        hide Comments About Role
        hide Comments About Molecular Function
        hide Other Comments
        Isolated in a yeast two-hybrid system, by screening for run-interacting proteins.
        (Golling et al., 1996)
        Cloned in a low stringency hybridisation screen using full length mouse PEBP2β as a probe. In vitro studies reveal Bro stimulates the DNA binding activity of the run domain. run can act as an active repressor in the presence of either partner protein (Bro or Bgb).
        (Fujioka et al., 1996)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/Swiss-Prot
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Core binding factor, beta subunit (IPR003417)
        TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles
        •
        • T02222
        Linkouts
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Bgb
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DEDB - Drosophila exon database: splicing graphs
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        FlyExpress - Embryonic expression images (BDGP data)
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        InParanoid orthologs - Eukaryotic orthologs
        •
        PANTHER - Protein classification by function, families, and pathways
        •
        hide Synonyms & Secondary IDs ( 8 )
        Reported As
        Symbol Synonym
        bgb
        (Wildonger and Mann, 2005, Kaminker et al., 1999, Fujioka et al., 1996)
        Bgb
         
        CG7959
         
        en(lz)D/9
        (Kaminker et al., 2001)
        rpβ2
        (Fujioka et al., 1996)
        Name Synonym
        Big brother
        (Golling, 1995.3.4, Golling et al., 1995)
        Big-brother
        (Gergen, 1995.3.27)
        runt domain partner β2
        (Fujioka et al., 1996)
        Secondary FlyBase IDs
          hide References ( 31 )
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