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Gene Dmel\Arf51F

General Information
Symbol	Dmel\Arf51F	Species	D. melanogaster
Name	ADP ribosylation factor 51F	Annotation symbol	CG8156
Feature type	protein_coding_gene	FlyBase ID	FBgn0013750
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	51F6-51F6	Sequence location	2R:11,209,979..11,212,399 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ADP ribosylation factor 51F is referred to in FlyBase by the symbol Arf51F (CG8156, FBgn0013750). It has the cytological map location 51F6. Its sequence location is 2R:11209979..11212399. Its molecular function is described as: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; GTPase activity; GTP binding. It is involved in the biological processes: protein amino acid ADP-ribosylation; neurotransmitter secretion; synaptic vesicle endocytosis; myoblast fusion; endosome transport; protein transport; small GTPase mediated signal transduction. 7 alleles are reported. The phenotype of these alleles is annotated with embryonic myoblast. It has 5 annotated transcripts and 5 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 51F6-51F6   Limits computationally determined from genome sequence between P{PZ}scb01288 and P{lacW}dupk03308
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51F-51F   (Lee et al., 1994)
Experimentally Determined Recombination Data
Location	2-
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Arf51F for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Arf51F-RA FBtr0087378  NM_079027   1381   175 Arf51F-RB FBtr0087376  NM_166088   1352   175 Arf51F-RC FBtr0087377  NM_166089   1399   175 Arf51F-RD FBtr0087375  NM_166090   1370   175 Arf51F-RE FBtr0087379  NM_166091   1350   175
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Lee et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Arf51F-PA   FBpp0086510   20.0   175   9.41     NP_523751   AAF58148 Arf51F-PB   FBpp0086508   20.0   175   9.41     NP_725452   AAM68532 Arf51F-PC   FBpp0086509   20.0   175   9.41     NP_725453   AAM68533 Arf51F-PD   FBpp0086507   20.0   175   9.41     NP_725454   AAM68534 Arf51F-PE   FBpp0086511   20.0   175   9.41     NP_725455   AAM68535
Additional Polypeptide Data & Comments
Reported size (kD)	175 (aa) (Lee et al., 1994)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0013750
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras GTPase (IPR001806) Small GTP-binding protein (IPR005225) ADP-ribosylation factor (IPR006688) ARF/SAR superfamily (IPR006689)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392123   AA817443   AE003811 AAF58148   AE003811 AAM68532   AE003811 AAM68533   AE003811 AAM68534   AE003811 AAM68535   AQ073726   AY071116 AAL48738   BH759359   BI163301   BI164629   BI166012   BI169192   BI173781   BI216362   BI216893   BI238507   BI240236   BI241212   BI243415   BI243514   BI357352   BI364005   BI364773   BI366235   BI366722   BI368855   BI370099   BI371178   BI373597   BI373670   BI375497   BI577234   BP545923   BP553555   BP553556   CO189118   CO267590   CO271685   CO272840   CO277706   CO300161   CO307532   CO307659   CO315800   CO343526   EC057896   EC061701   EC071625   EC077877   EC089207   EC197939   EC209891   EC238072   EC246057   EC259569   L25063 AAA28378   L25064 AAA53668
UniProtKB/Swiss-Prot	P40946
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	RE16882 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12425
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage  head (Lee et al., 1994)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA817443 AA950276
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0013750 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0013750 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0013750 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for Arf51F FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Arf51F Stage(s) 1-3 Stage(s) 4-6 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele embryonic myoblast, with Scer\GAL4kirre-rP298 Arf51FT27N.Scer\UAS ventral nerve cord commissure & embryo, with Scer\GAL4rho.PL Arf51FT27N.Scer\UAS
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Arf51F Class Mutagen Stocks Known lesion Arf51FEP2612 P-element activity 2 -- Arf51FKG02753 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Arf51F Class Mutagen Stocks Known lesion Arf51FGD13822 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Arf51FScer\UAS.cCa in vitro construct | regulatory fusion 0 Yes Arf51FT27N.Scer\UAS in vitro construct | site directed mutagenesis in vitro construct | regulatory fusion 0 Yes Arf51FT27N.Tub in vitro construct | site directed mutagenesis 0 Yes Arf51FTub.cCa in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)L4
Useful duplication	Dp(2;2)SMG45
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13822} NA P{UAS-Arf51F.C} NA P{UAS-Arf51F.T27N} NA characterization construct P{Tub-Arf51F.C} NA P{Tub-Arf51F.T27N} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}Arf51FKG02753 NA insertion of mobile activating element P{EP}Arf51FEP2612 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Arf51F is characterised: tissue distribution, gene structure and chromosome mapping. Functional properties of the recombinant protein is studied in vitro. (Lee et al., 1994)
Gene Ontology: Function, Process & Cellular Component ( 11 )
Molecular Function
CV term Evidence References
GTP binding inferred from sequence or structural similarity with MGD:Arf2; MGI:MGI:99595 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.2.1)
GTPase activity inferred from sequence or structural similarity (Jekely, 2003) non-traceable author statement (Swiss-Prot Project Members, 1995.2.1)
NAD(P)+-protein-arginine ADP-ribosyltransferase activity inferred from direct assay (Lee et al., 1994)
Biological Process
CV term Evidence References
endosome transport traceable author statement (Stimson and Ramaswami, 1999)
myoblast fusion inferred from mutant phenotype (Chen et al., 2003)
neurotransmitter secretion non-traceable author statement (Lloyd et al., 2000)
protein amino acid ADP-ribosylation inferred from direct assay (Lee et al., 1994) inferred from sequence or structural similarity with UniProtKB:P26438 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1995.2.1)
protein transport non-traceable author statement (Swiss-Prot Project Members, 1995.2.1)
small GTPase mediated signal transduction inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR006688 (FlyBase Curators et al., 2004-)
synaptic vesicle endocytosis traceable author statement (Lloyd et al., 2000)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR006688 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Arf51F allele Gene References Arf51FEP2612 pnr (Pena-Rangel et al., 2002)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62436 Drosophila PIMRider - The Drosophila Protein Interaction map • 521463
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13030 Dere\GG20511 Dgri\GH20425 Dmoj\GI19608 Dper\GL17751 Dpse\GA20856 Dsec\GM21603 Dsim\GD11108 Dvir\GJ14973 Dwil\GK20891 Dyak\GE13644
Curated drosophilid orthologs	Dpse\GA20856
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000021667 Anopheles gambiae (Ensembl) ENSBTAP00000034221 Bos taurus (Ensembl) ENSCAFP00000020964 Canis familiaris (Ensembl) ENSCINP00000011844 Ciona intestinalis (Ensembl) NEWSINFRUP00000180638 Takifugu rubripes (Ensembl) NEWSINFRUP00000181305 Takifugu rubripes (Ensembl) ENSGALP00000020012 Gallus gallus (Ensembl) ENSP00000298316 Homo sapiens (Ensembl) ENSMMUP00000020526 Macaca mulatta (Ensembl) ENSPTRP00000010720 Pan troglodytes (Ensembl) ENSRNOP00000006355 Rattus norvegicus (Ensembl) ENSXETP00000051732 Xenopus tropicalis (Ensembl) WBGene00023741 Caenorhabditis briggsae (Wormbase) WBGene00000184 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-1519 Danio rerio (Zfin) ZDB-GENE-030131-8007 Danio rerio (Zfin) MGI:99435 Mus musculus (Informatics) SPBC1539.08 Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Szeged	- P{EP}Arf51FEP2612
VDRC	v24224 w1118; P{GD13822}v24224
Bloomington	17076 w1118; P{EP}Arf51FEP2612/CyO 13763 y1 w67c23; P{SUPor-P}Arf51FKG02753
Genomic Clones ( 1 )
	BACR33K06
cDNA Clones ( 36 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE16882
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE06030 RE49025 RE60930 RE41175 RE38235 RE23479 RE52319 LD12493 RE56494 RE51732 RE34988 RE58022 RE36935 RE04341 RE41051 RE02662 RE54908 RE61030 RE49951 RE63335 RE43817 LD22876 RE10291 RE70886 RE24175
Other clones	EK255203 EK262460 EK043057 EK104261 EK254754 EK125961 EK177514 EK108528 EK150944 EP16962
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0013750 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0013750
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
	Encodes a small GTP-binding protein of the ADP-ribosymation factor family.
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_166090 NM_166088 NM_166089 NM_079027 NM_166091 RefSeq (Proteins) • NP_725454 NP_725452 NP_725453 NP_523751 NP_725455 DDBJ / EMBL / GenBank • AE003811 AAF58148 AAM68532 AAM68533 AAM68534 AAM68535 AQ073726