Gene Dmel\sced

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General Information

Symbol

Dmel\sced

Species

D. melanogaster

Name

scrambled

Annotation symbol

CG3273

Feature type

protein_coding_gene

FlyBase ID

FBgn0013732

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

42C3-42C3

Sequence location

2R:2,544,833..2,548,129 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene scrambled is referred to in FlyBase by the symbol sced (CG3273, FBgn0013732). It has the cytological map location 42C3. Its sequence location is 2R:2544833..2548129. Its molecular function is unknown. It is involved in the biological processes: actin filament reorganization during cell cycle; pseudocleavage during syncytial blastoderm formation. 8 alleles are reported. The phenotypes of these alleles are annotated with: cycle 9 embryo; cycle 10 embryo; cycle 11 embryo; cycle 12 embryo; cycle 13 embryo; cycle 14 embryo. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42C3-42C3

Limits computationally determined from genome sequence between P{lacW}geminin^k14019&P{PZ}Adf1⁰¹³⁴⁹ and P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42A-42B

(determined by in situ hybridisation) 42B3

(Stevenson et al., 2001)

43-43

(determined by in situ hybridisation)

(Sullivan et al., 1993)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\sced for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

sced-RA

FBtr0086110

NM_136375

3297

822

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

sced-PA

FBpp0085445

91.3

822

8.58

NP_610219

AAF57367

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q6NQZ5

Maps to

Does NOT map to

Identified with

LD23309

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

sced[+t3.9]
2R:2,544,143..2,548,087

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=HindIII-HindIII_rfrag

(Stevenson et al., 2001)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11647

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI107530

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013732

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for sced

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | maternal effect

sced¹

lethal | maternal effect | recessive

sced¹

Sterility

Allele

female sterile

fertile

Phenotype manifest in

Allele

cycle 9 embryo | maternal effect

sced¹

cycle 10 embryo | maternal effect

sced¹

cycle 11 embryo | maternal effect

sced¹

cycle 12 embryo | maternal effect

sced¹

cycle 13 embryo | maternal effect

sced¹

cycle 14 embryo | maternal effect

sced¹

cycle 11 embryo

sced¹

cycle 12 embryo

sced¹

cycle 13 embryo

sced¹

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of sced	Class	Mutagen	Known lesion
sced¹	loss of function	P-element activity	--
sced^Ex11		P-element activity	--
sced^Ex1		P-element activity	--
sced^Ex20		P-element activity	--
sced^Ex32		P-element activity	--
sced^unspecified			--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of sced	Class	Mutagen	Stocks	Known lesion
sced^+t3.9		in vitro construct \| genomic fragment	0	Yes
sced^GD10921		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)cn88b

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10921}

characterization construct

P{sced^+t3.9}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}sced^DG20201

P{}sced¹

Related Comments

Please look at the allele reports for the complete phenotype data

Defined in a screen of maternal-effect lethal mutations that cause defects in nuclear movement, organization or morphology during the syncytial embryonic divisions.

(Sullivan et al., 1993)

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

actin filament reorganization during cell cycle

inferred from mutant phenotype

(Stevenson et al., 2002)

inferred from mutant phenotype

(Stevenson et al., 2001)

pseudocleavage during syncytial blastoderm formation

inferred from mutant phenotype

(Stevenson et al., 2002)

Cellular Component

CV term

Evidence

References

centrosome

inferred from direct assay

(Stevenson et al., 2001)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

sced allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61463

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11059

Dere\GG23210

Dgri\GH21818

Dmoj\GI20789

Dper\GL10111

Dpse\GA25083

Dsec\GM20884

Dsim\GD10414

Dvir\GJ20523

Dwil\GK15787

Dyak\GE19064

Curated drosophilid orthologs

Dhyd\sced

(Stevenson et al., 2001)

Dpse\GA17107

Dvir\sced

(Stevenson et al., 2001)

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000013605
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v26166

w¹¹¹⁸; P{GD10921}v26166

v26167

w¹¹¹⁸; P{GD10921}v26167

Bloomington

21720

y¹ w^67c23; P{wHy}sced^DG20201

Genomic Clones ( 1 )

BACR01C10

cDNA Clones ( 27 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones