Gene Dmel\not

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General Information

Symbol

Dmel\not

Species

D. melanogaster

Name

non-stop

Annotation symbol

CG4166

Feature type

protein_coding_gene

FlyBase ID

FBgn0013717

Created / Updated

2003-12-01/2008-03-03

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

75D4-75D4

Sequence location

3L:18,611,901..18,615,231 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene non-stop is referred to in FlyBase by the symbol not (CG4166, FBgn0013717). It has the cytological map location 75D4. Its sequence location is 3L:18611901..18615231. Its molecular function is described as: ubiquitin-specific protease activity; protein binding; ubiquitin thiolesterase activity; zinc ion binding. It is involved in the biological processes: axon target recognition; glial cell development; protein deubiquitination; glial cell migration; ubiquitin-dependent protein catabolic process; histone deubiquitination; regulation of transcription. 18 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: nervous system; organ system; larval head segment; adult brain; peripheral nervous system; portion of tissue; adult; wing hair; adult segment; adult mesothoracic segment. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75D4-75D4

Limits computationally determined from genome sequence between P{PZ}W⁰⁵⁰¹⁴ and P{lacW}l(3)j14E7^j14E7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

75C1-75C2

Mapped by recombination analysis and deficiency complementation tests (details unspecified).

(Poeck et al., 2001)

75C1-75C4

(Poeck, 1999.8.23)

75C3-75C4

(determined by in situ hybridisation)

(Spradling et al., 1999)

75C1-75C4

(determined by in situ hybridisation)

(Martin et al., 1995)

63A3-63A4

75C1--2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

(Martin et al., 1995)

Left of (cM)

Right of (cM)

(Martin et al., 1995)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\not for information on other features

Comments on Gene Model

DGC clone LD43147 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

not-RB

FBtr0290259

2495

496

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

not-PB

FBpp0288698

56.4

496

8.03

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0013717

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, C2H2-like (IPR015880)

Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394)

Zinc finger, UBP-type (IPR001607)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

LD08565

(Spradling et al., 1999)

LD43147

(BDGP Project Members, 2000-)

Dm0634

(BDGP Project Members, 1994-1999, Spradling et al., 1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3658

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA820116
AI511668

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013717

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0013717

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0013717

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

lethal | partially | prepupal stage (with Df(3L)W4)

not¹

lethal | pupal stage | recessive

Other Phenotypes

Allele

wild-type

visible | somatic clone

not¹

neuroanatomy defective

not⁰²⁰⁶⁹

tissue polarity defective | recessive | somatic clone

not¹

tissue polarity defective | somatic clone

not¹

Phenotype manifest in

Allele

photoreceptor cell R1 & axon

photoreceptor cell R2 & axon

photoreceptor cell R3 & axon

photoreceptor cell R4 & axon

photoreceptor cell R5 & axon

photoreceptor cell R6 & axon

lamina anlage | precursor

larval inner optic anlage | precursor

larval outer optic anlage | precursor

axon & photoreceptor cell R2

not⁰²⁰⁶⁹

axon & photoreceptor cell R3

not⁰²⁰⁶⁹

axon & photoreceptor cell R4

not⁰²⁰⁶⁹

axon & photoreceptor cell R5

not⁰²⁰⁶⁹

photoreceptor cell & axon (with Df(3L)W4)

not¹

ommatidium | somatic clone

not¹

eye photoreceptor cell | somatic clone

not¹

marginal glial cell | somatic clone

not¹

epithelial glial cell | somatic clone

not¹

wing hair | somatic clone

not¹

photoreceptor cell & axon

not^unspecified

lamina

not^unspecified

lamina plexus

not^unspecified

medulla

not^unspecified

marginal glial cell

not^unspecified

epithelial glial cell

not^unspecified

medulla glial cell

not^unspecified

Classical Alleles ( 13 )

For All Classical Alleles Show

Allele of not	Class	Mutagen	Stocks	Known lesion
not⁰²⁰⁶⁹	amorph	P-element activity	1	Yes
not⁰⁶⁸⁰⁶		P-element activity	0	--
not¹	amorph	ethyl methanesulfonate	0	--
not^DG24306			1	--
not^EP3066		P-element activity	1	--
not^S008913		P-element activity	0	Yes
not^S138410		P-element activity	0	Yes
not^d04543		P-element activity	1	--
not^d05745		P-element activity	1	--
not^d06314		P-element activity	2	--
not^j4B8a		P-element activity	0	--
not^j9A6		P-element activity	0	--
not^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of not	Mutagen	Stocks	Known lesion
not^GD11236	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
not^{MtnA.T:Zzzz\FLAG,T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
not^Scer\UAS.cPa	in vitro construct \| regulatory fusion	0	Yes
not^T:Hsap\MYC	in vitro construct \| coding region fusion	0	Yes
not^hs.PP	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)W4

Useful duplication

Dp(3;3)M35

Disrupted in

Df(3L)BSC416

(Christensen et al., 2008.4.15)

Df(3L)Cat

(Martin et al., 1995, Addison et al., 1995)

Df(3L)Exel6134

(Ryder, 2004.4.26)

Df(3L)GR844^X22

(Addison et al., 1995)

Df(3L)W10

(BDGP Project Members, 1994-1999, Martin et al., 1995)

Df(3L)W4

(Spradling et al., 1999, BDGP Project Members, 1994-1999, Martin et al., 1995, Addison et al., 1995)

Not disrupted in

Df(3L)GR844^X31

(Addison et al., 1995)

Df(3L)GR844^X33

(Addison et al., 1995)

Df(3L)Δ215

(Addison et al., 1995)

Df(3L)Δ26

(Addison et al., 1995)

Df(3L)Δ3002

(Addison et al., 1995)

Df(3L)Δ32

(Addison et al., 1995)

Df(3L)Δ54

(Addison et al., 1995)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11236}

P{UAS-not.P}

characterization construct

P{not^T:Hsap\MYC}

heat-shock construct

P{hs-not.XE}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}not^DG24306

insertion of enhancer trap

P{PZ}not⁰²⁰⁶⁹

Yes

P{PZ}not⁰⁶⁸⁰⁶

insertion of mobile activating element

Related Comments

Please look at the allele reports for the complete phenotype data

Genetic mosaic analysis fails to reveal a retinal requirement for not. Phenotypic analysis suggests not is not required in the optic ganglia to specify the correct termination site of R1-R6 neurons.

(Martin et al., 1995)

Gene Ontology: Function, Process & Cellular Component ( 13 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from physical interaction with FLYBASE:Sgf11; FB:FBgn0036804

(Weake et al., 2008)

ubiquitin thiolesterase activity

inferred from electronic annotation with InterPro:IPR001394

(FlyBase Curators et al., 2004-)

ubiquitin-specific protease activity

inferred from sequence or structural similarity with Pfam:PF00443

(Poeck et al., 2001)

zinc ion binding

inferred from electronic annotation with InterPro:IPR001607, InterPro:IPR015880

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

axon target recognition