Gene Dmel\TfIIA-S

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General Information

Symbol

Dmel\TfIIA-S

Species

D. melanogaster

Name

Transcription-factor-IIA-S

Annotation symbol

CG5163

Feature type

protein_coding_gene

FlyBase ID

FBgn0013347

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

95C8-95C8

Sequence location

3R:19,716,109..19,716,778 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Transcription-factor-IIA-S is referred to in FlyBase by the symbol TfIIA-S (CG5163, FBgn0013347). It has the cytological map location 95C8. Its sequence location is 3R:19716109..19716778. Its molecular function is described as general RNA polymerase II transcription factor activity. It is involved in the biological processes: transcription initiation from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter. 11 alleles are reported. The phenotypes of these alleles are annotated with: eye; photoreceptor cell; photoreceptor cell R1; photoreceptor cell R6; photoreceptor cell R7. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95C8-95C8

Limits computationally determined from genome sequence between P{PZ}l(3)04684⁰⁴⁶⁸⁴ and P{PZ}Atg6⁰⁰⁰⁹⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95C6-95C8

(Zeidler, 1994.12.6)

Experimentally Determined Recombination Data

Location

(Zeidler, 1994.12.6)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: sba+ TfIIA-S+

(Zeidler and Mlodzik, 1997)

Gene Model & Products

Please see the GBrowse view of Dmel\TfIIA-S for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

TfIIA-S-RA

FBtr0084526

NM_079743

614

106

Additional Transcript Data & Comments

Reported size (kB)

0.6 (longest cDNA)

(Yokomori et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

TfIIA-S-PA

FBpp0083912

12.2

106

8.70

NP_524467

AAF56199

Additional Polypeptide Data & Comments

Reported size (kD)

12.5 (kD predicted)

(Bernstein et al., 1994)

106 (aa); 12 (kD predicted)

(Yokomori et al., 1994)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0013347

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Transcription initiation factor IIA, gamma subunit, C-terminal (IPR015871)

Transcription initiation factor IIA, gamma subunit, N-terminal (IPR015872)

Transcription initiation factor IIA, gamma subunit (IPR003194)

Transcription factor IIA, helical (IPR009083)

Transcription factor IIA, beta-barrel (IPR009088)

TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles

•

T02226

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P52656

UniProtKB/TrEMBL

Q8MYY8

Maps to

Does NOT map to

Identified with

RE44302

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

TfIIA-S[+t4]
3R:19,712,845..19,717,424

comment=A 4kb genomic EcoRI fragment that includes the TfIIA-S coding region and the site of insertion of P{FZ}F125; position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=EcoRI-EcoRI_rfrag

(Zeidler et al., 1996)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10541

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

nucleus

(Bernstein et al., 1994)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI063405

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0013347

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0013347

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for TfIIA-S

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for TfIIA-S

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | embryonic stage | recessive

TfIIA-S^E32

lethal | pharate adult stage | recessive

TfIIA-S^E73

semi-lethal, with Scer\GAL4^29BD

TfIIA-S^GS97

Other Phenotypes

Allele

wild-type

TfIIA-S^+t4

visible, with Scer\GAL4^c355

TfIIA-S^GS97

visible, with Scer\GAL4^29BD

TfIIA-S^GS97

Phenotype manifest in

Allele

eye

TfIIA-S^E73

photoreceptor cell

TfIIA-S^E73

photoreceptor cell R1

TfIIA-S^E73

photoreceptor cell R6

TfIIA-S^E73

photoreceptor cell R7

TfIIA-S^E73

eye, with Scer\GAL4^29BD

TfIIA-S^GS97

eye, with Scer\GAL4^c355

TfIIA-S^GS97

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of TfIIA-S	Class	Mutagen	Stocks	Known lesion
TfIIA-S^E31	amorph	Δ2-3	0	Yes
TfIIA-S^E32	amorph	Δ2-3	1	Yes
TfIIA-S^E62	amorph	Δ2-3	0	Yes
TfIIA-S^E69	amorph	Δ2-3	0	Yes
TfIIA-S^E73	hypomorph	Δ2-3	1	Yes
TfIIA-S^E89	amorph	Δ2-3	0	Yes
TfIIA-S^EP3285		P-element activity	1	--
TfIIA-S^GS97		Δ2-3	1	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of TfIIA-S	Mutagen	Stocks	Known lesion
TfIIA-S^+t4	in vitro construct \| genomic fragment	0	Yes
TfIIA-S^GD4733	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
TfIIA-S^hs.PZ	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)06624

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)06624

(Zeidler et al., 1996)

Df(3R)Exel9014

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4733}

characterization construct

P{dTFIIA-S}

heat-shock construct

P{hsp70-dTFIIA-S}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}EY02323

P{EP}TfIIA-S^EP3285

P{GSV1}TfIIA-S^GS97

Related Comments

Please look at the allele reports for the complete phenotype data

Mitotic recombination reveals TfIIA-S does not have a role in eye development.

(Zeidler and Mlodzik, 1993)

TfIIA-S has been isolated, molecularly cloned and expressed. Recombinant purified TfIIA-L and TfIIA-S can form a stable complex with Tbp on DNA, the recombinant protein can stimulate basal transcription and enhances transcriptional activation.

(Yokomori et al., 1994)

12.5kD small subunit of TFIIA is cloned and the properties of the recombinant protein are studied in vitro. The recombinant protein, in conjunction with human TFIIA, can support both promoter complex formation and transcriptional activated expression of TFIIA.

(Bernstein et al., 1994)

TfIIA-S is up-regulated and required during Ras-mediated photoreceptor determination.

(Zeidler et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

general RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with HGNC_gene:GTF2A2; HGNC:4647; OMIM:600519 AND inferred from sequence or structural similarity with SGD_LOCUS:TOA2; SGD:S0001541

(Aoyagi and Wassarman, 2000)

Biological Process

CV term

Evidence

References

regulation of transcription from RNA polymerase II promoter

non-traceable author statement

(Swiss-Prot Project Members, 1996.10.1)

transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC_gene:GTF2A2; HGNC:4647; OMIM:600519 AND inferred from sequence or structural similarity with SGD_LOCUS:TOA2; SGD:S0001541

(Aoyagi and Wassarman, 2000)

Cellular Component

CV term

Evidence

References

nucleus

non-traceable author statement

(Swiss-Prot Project Members, 1996.10.1)

transcription factor TFIIA complex

inferred from sequence or structural similarity with HGNC_gene:GTF2A2; HGNC:4647; OMIM:600519 AND inferred from sequence or structural similarity with SGD_LOCUS:TOA2; SGD:S0001541

(Aoyagi and Wassarman, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

TfIIA-S allele

Gene

References

TfIIA-S^E73

Ras85D

(Zeidler et al., 1996)

svp

(Zeidler et al., 1996)

sev

(Zeidler et al., 1996)

unspecified

(Singh et al., 2005)

tor

(Zeidler et al., 1996)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

67758

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18732

Dere\GG12397

Dgri\GH10565

Dmoj\GI23054

Dper\GL24263

Dpse\GA18701

Dsec\GM23533

Dsim\GD18345

Dvir\GJ24638

Dwil\GK11978

Dyak\GE23914

Curated drosophilid orthologs

Dpse\GA18701

Dvir\TfIIA-S

Linkouts

InParanoid orthologs - Eukaryotic orthologs

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ENSANGP00000013128
Anopheles gambiae (Ensembl)
ENSBTAP00000013602
Bos taurus (Ensembl)
ENSCAFP00000024508
Canis familiaris (Ensembl)
ENSP00000267869
Homo sapiens (Ensembl)
ENSMMUP00000024248
Macaca mulatta (Ensembl)
ENSMODP00000014575
Monodelphis domestica (Ensembl)
ENSPTRP00000012186
Pan troglodytes (Ensembl)
ENSRNOP00000014706
Rattus norvegicus (Ensembl)
GSTENP00003553001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000047620
Xenopus tropicalis (Ensembl)
Q5SDB6
Oryza sativa (Gramene)
Q65X27
Oryza sativa (Gramene)
Q7FPX2
Oryza sativa (Gramene)
Q94HL5
Oryza sativa (Gramene)
AT4G24440.2
Arabidopsis thaliana (Arabidopsis)
WBGene00039192
Caenorhabditis briggsae (Wormbase)
WBGene00013736
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-050522-513
Danio rerio (Zfin)
ZDB-GENE-050522-513
Danio rerio (Zfin)
DDB0216276
Dictyostelium discoideum (Dictybase)
MGI:1933289
Mus musculus (Informatics)
YKL058W
Saccharomyces cerevisiae (Yeastgenome)
SPCC553.11c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

200041

v12745

w¹¹¹⁸; P{GD4733}v12745

v12746

w¹¹¹⁸; P{GD4733}v12746/CyO

Bloomington

15415

y¹ w^67c23; P{EPgy2}EY02323

5436

w^*; P{neoFRT}82B TfIIA-S^E73/TM6B, Tb¹

5437

w^*; TfIIA-S^E32/TM3, P{Thb8-lacZ}WD1, Sb¹ Ser¹

Genomic Clones ( 1 )

BACR05C11

cDNA Clones ( 9 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones