A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Nca

General Information
SymbolDmel\NcaSpeciesD. melanogaster
NameNeurocalcinAnnotation symbolCG7641
Feature typeprotein_coding_geneFlyBase IDFBgn0013303
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map3-
Cytogenetic map76D8-76D8Sequence location3L:19,985,023..19,990,663 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Neurocalcin is referred to in FlyBase by the symbol Nca (CG7641, FBgn0013303). It has the cytological map location 76D8. Its sequence location is 3L:19985023..19990663. Its molecular function is described as calcium ion binding. It is involved in the biological process negative regulation of phosphorylation. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
76D8-76D8  
Limits computationally determined from genome sequence between P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4 and P{PZ}eRF100103  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
76F-76F
 
(Teng, 1994.10.11)
76F-76F
(determined by in situ hybridisation)  
(Teng et al., 1994)
Experimentally Determined Recombination Data
Location
3-
(Teng, 1994.10.11)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Nca for information on other features
detailed view FBtr0074910 FBtr0074901 FBtr0074902 FBtr0074900 FBtr0074909 FBtr0074908 FBpp0074679 FBpp0074669 FBpp0074670 FBpp0074671 FBpp0074677 FBpp0074678 FBti0075646 FBti0043441 FBti0017689 FBti0037705 FBti0050083
Comments on Gene Model
Evidence indicates that 5' UTR overlaps 5' UTR of downstream gene (CG7646); (based upon RE66104 )
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Nca-RA
FBtr0074902
 NM_176365
  858
  190
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Nca-PA  
FBpp0074671  
21.9  
190  
5.01  
  NP_788543
  AAF49082
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Recoverin (IPR001125)
Calcium-binding EF-hand (IPR002048)
EF-Hand type (IPR011992)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA951796
 
AE003515
AAF49082
 
AF083308
 
AF171701
 
AY071612
AAL49234
 
AY113623
 
BF486351
 
BI365292
 
BI483236
 
BI483249
 
BI564696
 
BI582469
 
BI586438
 
BI618051
 
BI627837
 
BP549863
 
BP549864
 
BP554039
 
BP554040
 
BP556561
 
BP556562
 
CO185923
 
CO194536
 
CO261807
 
CO262181
 
CO263129
 
CO271965
 
CO274447
 
CO276228
 
CO277722
 
CO279374
 
CO286972
 
CO290532
 
CO298245
 
CO298332
 
CO300196
 
CO301154
 
CO303240
 
CO311001
 
CO311046
 
CO314845
 
CO320254
 
CO321643
 
CO322014
 
CO327503
 
CO330289
 
CO342778
 
CO343328
 
EC057252
 
EC058842
 
EC062707
 
EC063232
 
EC063794
 
EC064759
 
EC067148
 
EC068712
 
EC077034
 
EC080499
 
EC081401
 
EC083052
 
EC084219
 
EC194705
 
EC208162
 
EC213509
 
EC219571
 
EC221999
 
EC232056
 
EC232604
 
EC233052
 
EC234686
 
EC242286
 
EC246342
 
U15735
AAA62152
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 1 )
        For All Classical Alleles Show

        Allele of NcaClassMutagenStocksKnown lesion
        NcaA502
          		0 --
          hide Alleles Carried on Transgenic Constructs ( 1 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of NcaClassMutagenStocksKnown lesion
          NcaGD104770 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          UAS construct
          P{GD10477}
          NA
          Insertions
          Type of insertions
          Name
          Expression data
          miscellaneous insertions
          PBac{5HPw+}NcaA502
          NA
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          Identification and characterisation of Nca. Nca is present in synapses of the brain and thoracic ganglia. Nca product is a calcium-binding protein.
          (Teng et al., 1994)
          hide Gene Ontology: Function, Process & Cellular Component ( 2 )
          hide Molecular Function
          CV term
          Evidence
          References
          calcium ion binding
          inferred from direct assay
          (Teng et al., 1994)
          non-traceable author statement
          (Teng, 1994.10.11)
          inferred from sequence or structural similarity with UniProtKB:Q06389
          (FlyBase, 1992-)
          non-traceable author statement
          (Swiss-Prot Project Members, 1995.6.1)
          hide Biological Process
          CV term
          Evidence
          References
          negative regulation of phosphorylation
          non-traceable author statement
          (Swiss-Prot Project Members, 1995.6.1)
          hide Cellular Component
          CV term
          Evidence
          References
          hide Sequence Ontology: Class of Gene
          protein_coding_gene
           
          nuclear_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          Nca allele
          Gene
          References
          hide External Data
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
          Linkouts
          InParanoid orthologs - Eukaryotic orthologs
          •
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 1 )
          Bloomington
          16130
          y1 w1118; PBac{5HPw+}A502
          hide Genomic Clones ( 2 )
          hide cDNA Clones ( 40 )
          Please Note
          This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          hide Antibody Information
          hide Other Information
          hide Discoverer
          hide Etymology
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for database merge of
          Additional comments
          hide Comments About Role
          hide Comments About Molecular Function
          hide Other Comments
          hide External Crossreferences & Linkouts
          Sequence Crossreferences
          RefSeq (Transcripts)
          •
          RefSeq (Proteins)
          •
          DDBJ / EMBL / GenBank
          •
          UniProtKB/Swiss-Prot
          •
          Other Crossreferences
          InterPro domains - A database of protein families, domains, and functional sites
          •
          Recoverin (IPR001125)
          Calcium-binding EF-hand (IPR002048)
          EF-Hand type (IPR011992)
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          DEDB - Drosophila exon database: splicing graphs
          •
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          •
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          •
          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
          •
          GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          InParanoid orthologs - Eukaryotic orthologs
          •
          PANTHER - Protein classification by function, families, and pathways
          •
          hide Synonyms & Secondary IDs ( 6 )
          Reported As
          Symbol Synonym
          CG7641
           
          nca
          (Teng, 1994.10.11, Faurobert et al., 1996, Teng et al., 1994)
          Nca
           
          Ncalc
          (Zeng et al., 2000)
          Name Synonym
          neurocalcin
          (Braunewell and Gundelfinger, 1999, Teng et al., 1994)
          Neurocalcin
           
          Secondary FlyBase IDs
          • FBgn0013306
          hide References ( 14 )
          Research paper
          Zeng et al., 2000, Nature 403(6771): 789--795
          naked cuticle encodes an inducible antagonist of Wnt signalling. [FBrf0125452]
          Faurobert et al., 1996, J. Biol. Chem. 271(17): 10256--10262
          Drosophila neurocalcin, a fatty acylated, Ca<up>2+</up>-binding protein that associates with membranes and inhibits in vitro phosphorylation of bovine rhodopsin. [FBrf0087096]
          Teng et al., 1994, J. Biol. Chem. 269(50): 31900--31907
          A highly conserved homologue of bovine neurocalcin in Drosophila melanogaster is a Ca<up>2+</up>-binding protein expressed in neuronal tissues. [FBrf0076714]
          Supplementary material
          Dietzl et al., 2007, Nature 448(7150):
          Supplementary Table 1. [FBrf0202579]
          Giot et al., 2003, Science 302(5651):
          Tables S1 to S7. [FBrf0166452]
          Review
          Braunewell and Gundelfinger, 1999, Cell Tissue Res. 295(1): 1--12
          Intracellular neuronal calcium sensor proteins: a family of EF-hand calcium-binding proteins in search of a function. [FBrf0106018]
          Personal communication to FlyBase
          Berkeley Drosophila Genome Project, 2006.4.26, BDGP TI submission.
          BDGP TI submission. [FBrf0188739]
          FlyBase analysis
          Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
          Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
          FlyBase, 1992-, FlyBase curation
          FlyBase curation. [FBrf0105495]
          DNA/RNA sequence record
          Teng, 1994.10.11, GenBank/EMBL/DDBJ: U15735
          Drosophila melanogaster neurocalcin homolog DrosNCa (nca) mRNA, complete cds. [FBrf0121318]
          Protein sequence record
          Swiss-Prot Project Members, 1995.6.1, UniProtKB: P42325
          NEUROCALCIN HOMOLOG. [FBrf0124026]
          Computer file
          BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
          Berkeley Drosophila Genome Project. [FBrf0125078]
          Automatic genome annotation
          FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]
          [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
          Curated genome annotation
          Milshina, 1999.11, [Annotations performed at the Celera Jamboreee.]
          [Annotations performed at the Celera Jamboreee.] [FBrf0126686]