A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\Ance

General Information
SymbolDmel\AnceSpeciesD. melanogaster
NameAngiotensin converting enzymeAnnotation symbolCG8827
Feature typeprotein_coding_geneFlyBase IDFBgn0012037
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2LRecombination map2-
Cytogenetic map 34E2-34E2 Sequence location2L:13,905,645..13,909,172 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Angiotensin converting enzyme is referred to in FlyBase by the symbol Ance (CG8827, FBgn0012037). It has the cytological map location 34E2. Its sequence location is 2L:13905645..13909172. Its molecular function is described as: peptidyl-dipeptidase A activity; zinc ion binding. It is involved in the biological processes: sperm individualization; spermatid nuclear differentiation; metamorphosis; proteolysis. 5 alleles are reported. The phenotypes of these alleles are annotated with: testis; spermatid; primary spermatocyte cyst; major mitochondrial derivative; minor mitochondrial derivative. It has 2 annotated transcripts and 2 annotated polypeptides.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
l(2)34Eb
Heteroallelic combinations escape about 10% with thin bristles; female fertile; male sterile.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
34E2-34E2  
Limits computationally determined from genome sequence between P{lacW}Sosk05224&P{lacW}RpII33k05605 and P{lacW}l(2)34Fak00811&P{PZ}wb09437  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
34E-34E
(determined by in situ hybridisation)  
(Tatei et al., 1995)
34A-34A
(determined by in situ hybridisation)  
(Cornell et al., 1995)
Experimentally Determined Recombination Data
Location
2-
 
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: Smg5- Ance+ Acyp+
(Ashburner et al., 1999)
Gene Order (overall orientation not stated)
References
Overall orientation not stated: Smg5- Ance+
(Tatei et al., 1995)
hide Gene Model & Products
Please see the GBrowse view of Dmel\Ance for information on other features
detailed view FBtr0080562 FBtr0080561 FBtr0080552 FBtr0080553 FBtr0080554 FBpp0080139 FBpp0080138 FBpp0080129 FBpp0080130 FBpp0080131 FBti0010906 FBti0066484 FBti0067110 FBti0043221 FBti0048599 FBti0065573 FBti0045441 FBti0025656
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Ance-RA
FBtr0080552
 NM_057698
  2006
  615
Ance-RB
FBtr0080553
 NM_165070
  1991
  615
Additional Transcript Data & Comments
Reported size (kB)
2.1 (northern blot)
(Cornell et al., 1995)
4.0, 2.0 (northern blot)
(Tatei et al., 1995)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Ance-PA  
FBpp0080129  
70.9  
615  
4.78  
  NP_477046
  AAF53353
Ance-PB  
FBpp0080130  
70.9  
615  
4.78  
  NP_723852
  AAN10856
Additional Polypeptide Data & Comments
Reported size (kD)
615 (aa); 67 (kD observed)
(Cornell et al., 1995)
615 (aa)
(Tatei et al., 1995)
Comments
Ance protein was purified from Drosophila embryos. Ance protein, expressed in COS cells, is a secreted enzyme,which converts angiotensin I to angiotensin II and is inhibited by captopril and trandolaprilat.
(Cornell et al., 1995)
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Peptidase M2, peptidyl-dipeptidase A (IPR001548)
Peptidase M, neutral zinc metallopeptidases, zinc-binding site (IPR006025)
MEROPS - An information resource for peptidases and the proteins that inhibit them
•
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA246993
 
AA392405
 
AA433287
 
AA536582
 
AA694939
 
AA695131
 
AC092223
 
AE003408
AAF44834
 
AE003641
AAF53353
 
AE003641
AAN10856
 
AI108085
 
AI260382
 
AI260736
 
AI260812
 
AI295251
 
AI295406
 
AI295438
 
AI295999
 
AI387659
 
AI404742
 
AI405496
 
AI515154
 
AI519344
 
AW942183
 
AY061129
AAL28677
 
BF488497
 
BF489057
 
BF489635
 
BF502630
 
BF506693
 
BG633956
 
BI170125
 
BI173144
 
BI173350
 
BI212702
 
BI212810
 
BI228882
 
BI229155
 
BI233074
 
BI235517
 
BI240362
 
BI362006
 
BI362012
 
BI363537
 
BI363612
 
BI371531
 
BI373791
 
BI482531
 
BI578925
 
BI579051
 
BI588377
 
BI612523
 
BI627190
 
BI630773
 
CK658914
 
CK660538
 
CK660660
 
CO181178
 
CO185695
 
CO194868
 
CO268498
 
CO282172
 
CO288629
 
CO301195
 
CO305734
 
CO306725
 
CO323752
 
EC051637
 
EC052638
 
EC054117
 
EC055952
 
EC057105
 
EC066170
 
EC203152
 
EC203451
 
EC208548
 
EC212024
 
EC213092
 
EC214922
 
EC222438
 
EC226686
 
EC231702
 
EC237845
 
EC242063
 
EC248157
 
EC249448
 
EL871543
 
EL871772
 
EL871905
 
EL878953
 
EL878954
 
EL879092
 
EL879093
 
L80114
 
U25344
AAB02171
 
U34599
AAC46902
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (Ashburner et al., 1999.10.12, Coates et al., 2000)
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (Ashburner et al., 1999.10.12)
    (Ashburner et al., 1999, Ashburner et al., 1999.10.12, BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    protein binding site
    Ance-protein_bind-2
    2L:13,905,145..13,905,161
    bound_moiety=zen-XP
    evidence=experimental
    (Rusch and Levine, 1997, Bergman et al., 2004)
    protein binding site
    Ance-protein_bind-3
    2L:13,905,234..13,905,259
    bound_moiety=zen-XP
    evidence=experimental
    (Bergman et al., 2004, Rusch and Levine, 1997)
    protein binding site
    Ance-protein_bind-4
    2L:13,905,030..13,905,048
    bound_moiety=zen-XP
    evidence=experimental
    (Rusch and Levine, 1997)
    regulatory region
    Ance-enhancer-1
    2L:13,904,733..13,905,265
    comment=The Ance enhancer element directs lacZ reporter expression in an endogenous pattern. The element was mapped to a ClaI-XhoI restriction fragment; position of restriction fragment on reference sequence inferred by FlyBase curator
    evidence=experimental
    linked_to=ClaI-XhoI_rfrag
    (Tatei et al., 1995)
    rescue fragment
    Ance[+t16.5]
    2L:13,896,515..13,912,233
    comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
    evidence=experimental
    linked_to=EcoRI-EcoRI_rfrag
    (Tatei et al., 1995)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage | stage >9
     posterior embryonic/larval midgut
    (Tatei et al., 1995)
     embryonic stage | stage stage >9
     anterior embryonic/larval midgut
    (Tatei et al., 1995)
     embryonic stage | late
     pericardial cell
    (Tatei et al., 1995)
     embryonic stage | 4-12 hr
     
    (Tatei et al., 1995)
     embryonic stage | stage 5-13
     amnioserosa
    (Tatei et al., 1995)
     embryonic stage-adult stage
     
    (Tatei et al., 1995)
     embryonic stage | late
     embryonic/larval dorsal vessel
    (Tatei et al., 1995)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Ance
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for Ance
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        semi-viable (with Ance34Eb-1)
        semi-viable (with Ance34Eb-2)
        lethal | recessive
        Other Phenotypes
        Allele
        wild-type
        Sterility
        Allele
        male sterile
        male sterile (with Ance34Eb-1)
        male sterile (with Ance34Eb-2)
        Phenotype manifest in
        Allele
        testis (with Ance34Eb-2)
        testis (with Df(2L)Adh-nBR55)
        spermatid (with Df(2L)Adh-nBR55)
        spermatid & nucleus (with Df(2L)Adh-nBR55)
        primary spermatocyte cyst (with Df(2L)Adh-nBR55)
        major mitochondrial derivative (with Df(2L)Adh-nBR55)
        minor mitochondrial derivative (with Df(2L)Adh-nBR55)
        testis (with Df(2L)b88f32)
        testis
        testis (with Ance34Eb-1)
        hide Classical Alleles ( 2 )
        For All Classical Alleles Show

        Allele of AnceClassMutagenStocksKnown lesion
        Ance34Eb-11 --
        Ance34Eb-21 --
        hide Alleles Carried on Transgenic Constructs ( 3 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of AnceClassMutagenStocksKnown lesion
        Ance+t16.50 Yes
        AnceGD53881 Yes
        Ancemt3530 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Disrupted in
        (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994, Roote et al., 1996, Roote, 1996.8, Roote, 1997.6.3)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Roote, 1995.3.24, Tatei et al., 1995, Roote, 1997.6.3)
        (Christensen and Cook, 2007.3.22)
        (Christensen and Cook, 2007.3.22)
        (Gubb et al., 1990)
        (Roote, 1995.3.27, Roote et al., 1996, Roote, 1997.6.3)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Roote, 1995.3.27)
        (Gubb et al., 1990)
        (Iyengar et al., 1999, Ashburner et al., 1982)
        (Roote, 1995.3.27)
        (Roote, 1995.3.27)
        (Tatei et al., 1995)
        (Roote, 1995.3.27, Tatei et al., 1995)
        (Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 1995.3.27)
        Not disrupted in
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Ashburner et al., 1982, Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (Fossett et al., 1994)
        (