Gene Dmel\E2f

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General Information

Symbol

Dmel\E2f

Species

D. melanogaster

Name

E2F transcription factor

Annotation symbol

CG6376

Feature type

protein_coding_gene

FlyBase ID

FBgn0011766

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

93E9-93F1

Sequence location

3R:17,446,026..17,486,127 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene E2F transcription factor is referred to in FlyBase by the symbol E2f (CG6376, FBgn0011766). It has the cytological map location 93E9-93F1. Its sequence location is 3R:17446026..17486127. Its molecular function is described as: DNA binding; RNA polymerase II transcription factor activity; protein binding; transcription activator activity; transcription factor activity. It is involved in the biological processes described with 14 unique terms, many of which group under: cell cycle; regulation of metabolic process; regulation of biological process; anatomical structure development; cell cycle process; ovarian nurse cell to oocyte transport; immune response; positive regulation of cell proliferation; transcription, DNA-dependent; positive regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; growth; regulation of apoptosis; cellular process. 60 alleles are reported. The phenotypes of these alleles are annotated with 28 unique terms, many of which group under: peripheral nervous system; organ system; nervous system; imaginal precursor; primordium; egg; embryonic nervous system; cell part; ventral nerve cord primordium; rhabdomere; neuroblast NB6-4 of abdomen; embryonic heart; cell cycle; dorsal thoracic disc. It has 3 annotated transcripts and 3 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93E9-93F1

Limits computationally determined from genome sequence between P{PZ}InR⁰⁵⁵⁴⁵ and P{PZ}tsl⁰⁰⁶¹⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93E8-93E9

(determined by in situ hybridisation)

(Spradling et al., 1999)

93E-93E

(determined by in situ hybridisation)

(Seum et al., 1996)

93E-93E

(determined by in situ hybridisation)

(Duronio et al., 1995)

93E-93E

(determined by in situ hybridisation)

(Spierer et al., 1995)

86C3-86C4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: DNApol-α180+ Ori93Fα? E2f+ Ori93Fβ? E2f+

(Sasaki et al., 1999)

Overall orientation not stated: DNApol-α180+ E2f+

(Sasaki, 1998.3.3)

Gene Model & Products

Please see the GBrowse view of Dmel\E2f for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

E2f-RA

FBtr0084119

NM_079713

4926

805

E2f-RB

FBtr0084117

NM_169961

4187

805

E2f-RC

FBtr0084118

NM_169962

4389

805

Additional Transcript Data & Comments

Reported size (kB)

4.7 (northern blot)

(Sawado et al., 1998)

4.5 (longest cDNA)

(Dynlacht et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

E2f-PA

FBpp0083518

87.5

805

6.64

NP_524437

AAF55904

E2f-PB

FBpp0083516

87.5

805

6.64

NP_732646

AAN13878

E2f-PC

FBpp0083517

87.5

805

6.64

NP_732647

AAN13879

Additional Polypeptide Data & Comments

Reported size (kD)

805 (aa)

(Dynlacht et al., 1994)

805 (aa); 88 (kD predicted)

(Ohtani and Nevins, 1994)

Comments

Cotransfection assays in S2 cells were used to demonstrate that E2f protein and Dp protein form a heteromeric complex and cooperate to give sequence-specific DNA binding and transcriptional activation. Deletion mutants of E2f protein were made to identify a region of E2f protein necessary for interaction with Dp protein.

(Dynlacht et al., 1994)

E2f2 protein requires Dp protein for binding to the E2f recognition sites of the adenovirus E2 promoter. E2f protein does not require Dp protein for binding the same site, but binding is enhanced in the presence of Dp protein. E2f2 binds to one of two E2f recognition sites in the mus209 gene promoter. Unlike the transcription activator E2f, E2f2 acts as a repressor of expression from the mus209 promoter.

(Sawado et al., 1998)

E2f protein expressed in bacteria was shown to have DNA-binding activity and to bind specifically to E2f recognition sequences. In S2 cells, E2f protein stimulates transcription and the activation is dependent on functional E2f binding sites. The transcription activation function was mapped to the C-terminal portion of the E2f protein between amino acids 560 and 769. Finally, E2f protein recognition sequences were found within the promoter of the DNA polymerase alpha gene.

(Ohtani and Nevins, 1994)

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011766

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Transcription factor E2F/dimerisation partner (TDP) (IPR003316)

Winged helix repressor DNA-binding (IPR011991)

E2F Family (IPR015633)

TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles

•

T01547

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q27368

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

GM02934

(Spradling et al., 1999)

Dm0325

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

E2f[91]
3R:17,452,129..17,452,129

evidence=experimental
na_change=C17452129T
pr_change=Q31|E2f-PA,Q31|E2f-PC,Q31@|E2f-PB
reported_na_change=C91T
reported_pr_change=Q31@

(Duronio et al., 1995)

point mutation

E2f[296]
3R:17,451,558..17,451,558

evidence=experimental
na_change=C17451558T
pr_change=Q178|E2f-PA,Q178|E2f-PC,Q178@|E2f-PB
reported_na_change=C532T
reported_pr_change=Q178@

(Duronio et al., 1995)

point mutation

E2f[i1]
3R:17,451,204..17,451,204

evidence=experimental
na_change=G17451204A
pr_change=D296N|E2f-PA,D296N|E2f-PC,D296N|E2f-PB
reported_na_change=G?A
reported_pr_change=D296N

(Royzman et al., 1999)

point mutation

E2f[i2]
3R:17,449,245..17,449,245

evidence=experimental
na_change=C17449245T
pr_change=Q527|E2f-PA,Q527|E2f-PC,Q527@|E2f-PB
reported_na_change=C?T
reported_pr_change=Q527@

(Royzman et al., 1999)

point mutation

E2f[su89]
3R:17,447,352..17,447,352

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=T17447352A
pr_change=L786Q|E2f-PA,L786Q|E2f-PC,L786Q|E2f-PB
reported_pr_change=L786Q

(Weng et al., 2003)

protein binding site

E2f-protein_bind-1
3R:17,460,089..17,460,094

bound_moiety=cad-XP
comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut.
evidence=experimental

(Hwang et al., 2002)

protein binding site

E2f-protein_bind-2
3R:17,459,867..17,459,873

bound_moiety=cad-XP
comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut.
evidence=experimental

(Hwang et al., 2002)

protein binding site

E2f-protein_bind-3
3R:17,459,780..17,459,785

bound_moiety=cad-XP
comment=cad-binding site is required for E2f expression in the posterior midgut and hindgut.
evidence=experimental