Gene Dmel\ana

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General Information

Symbol

Dmel\ana

Species

D. melanogaster

Name

anachronism

Annotation symbol

CG8084

Feature type

protein_coding_gene

FlyBase ID

FBgn0011746

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

45A1-45A1

Sequence location

2R:4,956,606..4,965,059 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene anachronism is referred to in FlyBase by the symbol ana (CG8084, FBgn0011746). It has the cytological map location 45A1. Its sequence location is 2R:4956606..4965059. Its molecular function is described as protein binding. It is involved in the biological process negative regulation of neuroblast proliferation. 15 alleles are reported. The phenotypes of these alleles are annotated with: neuroblast; thoracic neuroblast. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45A1-45A1

Limits computationally determined from genome sequence between P{lacW}babo^k16912 and P{lacW}l(2)k16529^k16529

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45A-45B

(determined by in situ hybridisation)

(Ebens et al., 1993)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\ana for information on other features

Comments on Gene Model

DGC clone appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ana-RA

FBtr0088559

NM_057690

2413

474

Additional Transcript Data & Comments

Reported size (kB)

2.4, 1.7 (northern blot)

(Ebens et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ana-PA

FBpp0087642

54.0

474

7.53

NP_477038

AAF58998

Additional Polypeptide Data & Comments

Reported size (kD)

474 (aa); 55 (kD)

(Ebens et al., 1993)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WW/Rsp5/WWP (IPR001202)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q26307

UniProtKB/TrEMBL

Q95TB3

Maps to

DS07727

(FlyBase, 1992-)

Does NOT map to

Identified with

(Oliver, 2001.8.16)

GH07389

(BDGP Project Members, 2000-, Oliver, 2001.8.16)

RH58463

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13253

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage

optic lobe

(Ebens et al., 1993)

larval stage

larval central nervous system | restricted

(Ebens et al., 1993)

larval stage

glial cell

(Ebens et al., 1993)

larval stage

larval brain

(Ebens et al., 1993)

embryonic stage | stage >13

embryonic central nervous system | restricted

(Ebens et al., 1993)

larval stage

ventral ganglion

(Ebens et al., 1993)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage >13

embryonic central nervous system | restricted

(Ebens et al., 1993)

larval stage

larval central nervous system | restricted

(Ebens et al., 1993)

larval stage

optic lobe

(Ebens et al., 1993)

larval stage

larval brain

(Ebens et al., 1993)

larval stage

ventral ganglion

(Ebens et al., 1993)

larval stage

glial cell

(Ebens et al., 1993)

Marker for

Subcellular Localization

CV Term

secretory vesicle

(Ebens et al., 1993)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI108915

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011746

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011746

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011746

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Other Phenotypes

Allele

wild-type

olfaction defective | recessive

Phenotype manifest in

Allele

neuroblast

ana¹

thoracic neuroblast

ana⁸

optic lobe & neuroblast

ana⁸

Classical Alleles ( 11 )

For All Classical Alleles Show

Allele of ana	Class	Mutagen	Stocks	Known lesion
ana¹	loss of function	Δ2-3 P-element activity	2	Yes
ana²	loss of function	Δ2-3	0	Yes
ana³	loss of function	Δ2-3	0	Yes
ana⁴	loss of function	Δ2-3	0	Yes
ana⁵	loss of function	Δ2-3	0	Yes
ana⁶	loss of function	Δ2-3	0	Yes
ana⁷	hypomorph	Δ2-3	0	Yes
ana⁸	amorph, loss of function	Δ2-3	0	Yes
ana⁹	loss of function	Δ2-3	0	Yes
ana^DG12701		P-element activity	1	--
ana^e02258		piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ana	Mutagen	Stocks	Known lesion
ana^+mEa	in vitro construct \| minigene in vitro construct \| genomic fragment	1	Yes
ana^+t9.3	in vitro construct \| genomic fragment	0	Yes
ana^GD5775	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
ana^hs.P	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

In(2LR)w45-32n

Disrupted in

Df(2R)Np1

(Ebens et al., 1993)

Df(2R)Np6

(Ebens et al., 1993)

Not disrupted in

Df(2R)w45-31n

(Ebens et al., 1993)

Df(2R)w45-47n

(Ebens et al., 1993)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD5775}

characterization construct

P{ana⁺9.3}

P{ana^+m}

heat-shock construct

P{ana^hs.P}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}ana^e02258

P{wHy}ana^DG12701

insertion of enhancer trap

P{A92}ana¹

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from electronic annotation with InterPro:IPR001202

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

negative regulation of neuroblast proliferation

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Cellular Component

CV term

Evidence

References

extracellular region

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ana allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61751

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12224

Dere\GG23405

Dgri\GH21635

Dmoj\GI20089

Dper\GL17613

Dpse\GA20811

Dsec\GM21087

Dsim\GD10624

Dvir\GJ19855

Dwil\GK17993

Dyak\GE19247

Curated drosophilid orthologs

Dpse\GA20811

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010885
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

8926

8927

P{ana^+m}1, w^*; P{A92}ana¹

20612

y¹ w^67c23; P{wHy}ana^DG12701

Genomic Clones ( 2 )

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH07389

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0011746

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0011746

Antibody Information

monoclonal, polyclonal

(Ebens et al., 1993)

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Required for neuroblast proliferation but expressed in glial cells.

The ana locus controls the proliferation of neuroblasts, but is expressed in glia. In mutants, quiescent neuroblasts enter S phase precociously.

(Ebens et al., 1993)

trol acts downstream of ana, it is possible that trol may function to inactivate or bypass ana repression to activate cell proliferation.

(Datta, 1995)

ana encodes a secreted glial glycoprotein that represses neuroblast proliferation in the larval brain.

(Datta, 1995)

Comments About Molecular Function

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

(Claridge-Chang et al., 2001)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_057690

RefSeq (Proteins)

•

NP_477038

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q26307

UniProtKB/TrEMBL

•

Q95TB3

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WW/Rsp5/WWP (IPR001202)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

61751

DEDB - Drosophila exon database: splicing graphs

•

13253

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0011746

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011746

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011746

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0011746

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011746

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0011746

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000010885
Anopheles gambiae (Ensembl)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/neural/anacron.htm

University of Chicago Dev. Expression

•

FBgn0011746

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

ana

(Barmina et al., 2005, Jacobsen et al., 2006, Hueber et al., 2007)

CG8084

med

(Ebens et al., 1991, )

Name Synonym

anachronism

(Barmina et al., 2005)

medullaless

Secondary FlyBase IDs

References ( 37 )

Generate a list of

All references relating to this gene ( 37 )

List References by type

Recent research papers ( 2 )

Hueber et al., 2007, Development 134(2): 381--392: Comparative analysis of Hox downstream genes in Drosophila. [FBrf0193798]
Jacobsen et al., 2006, Genetics 174(4): 1973--1982: Functional analysis of genes differentially expressed in the Drosophila wing disc: role of transcripts enriched in the wing region. [FBrf0194659]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2006

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