Gene Dmel\Arp66B

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General Information

Symbol

Dmel\Arp66B

Species

D. melanogaster

Name

Actin-related protein 66B

Annotation symbol

CG7558

Feature type

protein_coding_gene

FlyBase ID

FBgn0011744

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

66B6-66B6

Sequence location

3L:8,106,588..8,109,000 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Actin-related protein 66B is referred to in FlyBase by the symbol Arp66B (CG7558, FBgn0011744). It has the cytological map location 66B6. Its sequence location is 3L:8106588..8109000. Its molecular function is described as: structural constituent of cytoskeleton; actin binding; ATP binding. It is involved in the biological processes: cytoskeleton organization and biogenesis; actin filament reorganization during cell cycle; pseudocleavage during syncytial blastoderm formation; cytoplasmic transport, nurse cell to oocyte; ovarian ring canal stabilization; axonal fasciculation; regulation of actin polymerization and/or depolymerization; phagocytosis, engulfment; regulation of actin filament polymerization. 3 alleles are reported. The phenotypes of these alleles are annotated with: ventral nerve cord commissure; nurse cell; oocyte; nurse cell ring canal. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66B6-66B6

Limits computationally determined from genome sequence between P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118 and P{lacW}Nmt^j1C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66B-66B

(Fyrberg, 1993.5.4)

66B-66B

(determined by in situ hybridisation)

(Fyrberg et al., 1994)

66B-66B

(determined by in situ hybridisation)

(Fyrberg and Fyrberg, 1993)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Arp66B for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Arp66B-RA

FBtr0076737

NM_079244

1900

418

Additional Transcript Data & Comments

Reported size (kB)

1.257 (longest cDNA)

(Fyrberg et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Arp66B-PA

FBpp0076460

47.0

418

5.84

NP_523968

AAF50488

Additional Polypeptide Data & Comments

Reported size (kD)

418 (aa)

(Fyrberg et al., 1994)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011744

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin/actin-like (IPR004000)

Actin-related protein 3 (IPR015623)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P32392

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD35711

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

4812

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

oogenesis stage | stage S9

nurse cell ring canal

(Hudson and Cooley, 2002)

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI518886

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011744

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011744

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011744

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pharate adult stage | recessive, with Scer\GAL4[-]:

Arp66B^EP3640

Phenotype manifest in

Allele

ventral nerve cord commissure (with Df(3L)pbl-X1), with Scer\GAL4[-]:

Arp66B^EP3640

nurse cell | germ-line clone, with Scer\GAL4[-]:

Arp66B^EP3640

oocyte | germ-line clone, with Scer\GAL4[-]:

Arp66B^EP3640

nurse cell ring canal | germ-line clone, with Scer\GAL4[-]:

Arp66B^EP3640

nurse cell & actin filament | germ-line clone, with Scer\GAL4[-]:

Arp66B^EP3640

anterior dorsocentral bristle & pharate adult, with Scer\GAL4[-]:

Arp66B^EP3640

macrochaeta & pharate adult, with Scer\GAL4[-]:

Arp66B^EP3640

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Arp66B	Class	Mutagen	Stocks	Known lesion
Arp66B^EP3640		P-element activity	2	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Arp66B	Class	Mutagen	Stocks	Known lesion
Arp66B^GD12273		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Arp66B^{Scer\UAS.P\T.T:Avic\GFP}		in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)pbl-X1

Useful duplication

Dp(3;3)M67C⁺2

Disrupted in

Df(3L)Exel6112

(Ryder, 2004.4.26)

Df(3L)pbl-X1

(Fyrberg et al., 1994)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12273}

P{UASp-Arp66B.GFP}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Arp66B^EP3640

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

Isolation and characterisation of actin-related proteins Arp14D, Arp53D, Arp66B and Arp87C.

(Fyrberg et al., 1994)

Gene Ontology: Function, Process & Cellular Component ( 15 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR015623

(FlyBase Curators et al., 2004-)

actin binding

inferred from sequence or structural similarity with SGD_LOCUS:ARP3; SGD:S0003826

(FlyBase, 1992-)

structural constituent of cytoskeleton

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Biological Process

CV term

Evidence

References

actin filament reorganization during cell cycle

inferred from physical interaction with FLYBASE:Sop2; FB:FBgn0001961

(Stevenson et al., 2002)

axonal fasciculation

inferred from mutant phenotype

(Zallen et al., 2002)

cytoplasmic transport, nurse cell to oocyte

inferred from mutant phenotype

(Hudson and Cooley, 2002)

cytoskeleton organization and biogenesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

inferred from sequence or structural similarity with SGD_LOCUS:ARP3; SGD:S0003826

(FlyBase, 1992-)

ovarian ring canal stabilization

inferred from mutant phenotype

(Hudson and Cooley, 2002)

phagocytosis, engulfment

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

pseudocleavage during syncytial blastoderm formation

inferred from physical interaction with FLYBASE:Sop2; FB:FBgn0001961

(Stevenson et al., 2002)

regulation of actin filament polymerization

inferred from electronic annotation with InterPro:IPR015623

(FlyBase Curators et al., 2004-)

regulation of actin polymerization and/or depolymerization

non-traceable author statement

(Swiss-Prot Project Members, 1993.10.1)

Cellular Component

CV term

Evidence

References

Arp2/3 protein complex

non-traceable author statement

(Fyrberg, 1993.5.4)

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Vartiainen and Machesky, 2004)

inferred from sequence or structural similarity with SGD_LOCUS:ARP3; SGD:S0003826

(FlyBase, 1992-)

actin cytoskeleton

non-traceable author statement

(Swiss-Prot Project Members, 1993.10.1)

actin filament

inferred from sequence or structural similarity with EMBL:M81068

(Fyrberg et al., 1994)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Arp66B allele

Gene

References

Arp66B^EP3640

Sop2

(Zallen et al., 2002)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64322

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10484

Dere\GG14922

Dgri\GH15291

Dmoj\GI12000

Dper\GL22484

Dpse\GA20439

Dsec\GM24973

Dsim\GD17591

Dvir\GJ13271

Dwil\GK19880

Dyak\GE20375

Curated drosophilid orthologs

Dpse\GA20439

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009943
Anopheles gambiae (Ensembl)
ENSBTAP00000004410
Bos taurus (Ensembl)
ENSCAFP00000007401
Canis familiaris (Ensembl)
ENSCINP00000016926
Ciona intestinalis (Ensembl)
NEWSINFRUP00000155736
Takifugu rubripes (Ensembl)
ENSGALP00000010150
Gallus gallus (Ensembl)
ENSP00000263238
Homo sapiens (Ensembl)
ENSMMUP00000019703
Macaca mulatta (Ensembl)
ENSMODP00000000734
Monodelphis domestica (Ensembl)
ENSPTRP00000021215
Pan troglodytes (Ensembl)
ENSRNOP00000004520
Rattus norvegicus (Ensembl)
GSTENP00033110001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000033431
Xenopus tropicalis (Ensembl)
Q6K908
Oryza sativa (Gramene)
AT1G13180.1
Arabidopsis thaliana (Arabidopsis)
WBGene00026942
Caenorhabditis briggsae (Wormbase)
WBGene00000199
Caenorhabditis elegans (Wormbase)
ZDB-GENE-040428-2
Danio rerio (Zfin)
DDB0219936
Dictyostelium discoideum (Dictybase)
MGI:1921367
Mus musculus (Informatics)
YJR065C
Saccharomyces cerevisiae (Yeastgenome)
SPAC630.03
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Szeged

P{EP}Arp66B^EP3640

VDRC

v35258

w¹¹¹⁸; P{GD12273}v35258

v35260

w¹¹¹⁸; P{GD12273}v35260

Bloomington

17149

w¹¹¹⁸; P{EP}Arp66B^EP3640/TM6B, Tb¹

Genomic Clones ( 2 )

cDNA Clones ( 59 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones