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Gene Dmel\spdo

General Information
Symbol	Dmel\spdo	Species	D. melanogaster
Name	sanpodo	Annotation symbol	CG31020
Feature type	protein_coding_gene	FlyBase ID	FBgn0011716
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99F6-99F6	Sequence location	3R:26,302,411..26,304,311 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene sanpodo is referred to in FlyBase by the symbol spdo (CG31020, FBgn0011716). It has the cytological map location 99F6. Its sequence location is 3R:26302411..26304311. Its molecular function is described as: Notch binding; protein binding. It is involved in the biological processes: peripheral nervous system development; Notch signaling pathway; asymmetric cell division; sensory organ development. 19 alleles are reported. The phenotypes of these alleles are annotated with 49 unique terms, many of which group under: peripheral nervous system; nervous system; embryonic nervous system; hypodermal muscle of larval abdomen; organ system; embryonic neuron; abdominal oblique muscle; larval abdominal segment; larval abdominal segment 5; commissure. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99F6-99F6   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99F1-100B5 (determined by in situ hybridisation)   (Salzberg et al., 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\spdo for information on other features
Comments on Gene Model
	3' terminus extended to first stop codon; no experimental data confirming this prediction
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spdo-RA FBtr0085651  NM_170493   1822   565
Additional Transcript Data & Comments
Reported size (kB)	7.9, 7.3, 5.8, 3.6, 3.1, 2.7 (northern blot) (Dye et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein spdo-PA   FBpp0085013   63.8   565   8.71     NP_733372   AAN14235
Additional Polypeptide Data & Comments
Reported size (kD)	367 (aa) (Dye et al., 1998) 367, 45 (aa); 403, 43 (kD) (Mardahl-Dumesnil and Fowler, 2001)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0011716
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003774 AAN14235   BI164912   BI231733   BT001576 AAN71331   CZ467313   CZ467324   CZ468708   CZ470564   CZ478701   CZ483140   CZ486118   CZ487584   CZ488298   EC211423   EC246757
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IGV5 Q8IMI5
Maps to
Does NOT map to
Identified with	RE23355 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References deletion spdo[AC85] 3R:26,303,138..26,303,542 comment=In-frame deletion of amino acids 202-336. evidence=experimental (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[G104] 3R:26,302,957..26,302,957 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T26302957R pr_change=Y141@|spdo-PA reported_pr_change=Y141@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[AC81] 3R:26,303,108..26,303,108 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C26303108T pr_change=Q192@|spdo-PA reported_pr_change=Q192@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[VV86] 3R:26,303,267..26,303,267 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C26303267T pr_change=R245@|spdo-PA reported_pr_change=R245@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[ZZ213] 3R:26,303,324..26,303,324 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C26303324T pr_change=Q264@|spdo-PA reported_pr_change=Q264@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[P46] 3R:26,303,468..26,303,468 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C26303468T pr_change=R312@|spdo-PA reported_pr_change=R312@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[Z143] 3R:26,303,777..26,303,777 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=A26303777T pr_change=K415@|spdo-PA reported_pr_change=K415@ (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[OO3] 3R:26,303,870..26,303,870 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G26303870M pr_change=G446R|spdo-PA reported_pr_change=G446R (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[AB153] 3R:26,303,870..26,303,870 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G26303870M pr_change=G446R|spdo-PA reported_pr_change=G446R (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[K433] 3R:26,304,016..26,304,016 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C26304016T pr_change=S468F|spdo-PA reported_pr_change=S468F (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003) point mutation spdo[C55] 3R:26,304,124..26,304,124 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T26304124G pr_change=L504R|spdo-PA reported_pr_change=L504R (Skeath, 2005.2.14, O'Connor-Giles and Skeath, 2003)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 11370
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  larval stage-adult stage   (Dye et al., 1998)  embryonic stage | stage >=12  embryonic peripheral nervous system (Dye et al., 1998)  embryonic stage-adult stage   (Dye et al., 1998)  embryonic stage | stage 14-16  trichogen cell (Dye et al., 1998)  embryonic stage | >=9-12 hr   (Dye et al., 1998)  larval stage-pupal stage   (Dye et al., 1998)  embryonic stage | >=9-12 hr   (Dye et al., 1998)  larval stage-pupal stage   (Dye et al., 1998)  embryonic stage | >=9-12 hr   (Dye et al., 1998)  embryonic stage | stage >=9-11  embryonic central nervous system | precursor (Dye et al., 1998)  larval stage-pupal stage   (Dye et al., 1998)  embryonic stage | >=9-12 hr   (Dye et al., 1998)  embryonic stage | stage 14-16  tormogen cell (Dye et al., 1998)  pupal stage   (Dye et al., 1998)  embryonic stage   (Dye et al., 1998)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  adult stage | male   (Mardahl-Dumesnil and Fowler, 2001)  adult stage  adult abdomen (Mardahl-Dumesnil and Fowler, 2001)  adult stage  adult head (Mardahl-Dumesnil and Fowler, 2001)  adult stage| female   (Mardahl-Dumesnil and Fowler, 2001)  adult stage   (Mardahl-Dumesnil and Fowler, 2001)  pupal stage  myofibril (Mardahl-Dumesnil and Fowler, 2001)  embryonic stage-adult stage   (Mardahl-Dumesnil and Fowler, 2001)  adult stage | day 1  myofibril (Mardahl-Dumesnil and Fowler, 2001)  adult stage  indirect flight muscle (Mardahl-Dumesnil and Fowler, 2001)  adult stage  thin filament (Mardahl-Dumesnil and Fowler, 2001)  pupal stage, adult stage  thin filament (Mardahl-Dumesnil and Fowler, 2001)  adult stage  adult thorax (Mardahl-Dumesnil and Fowler, 2001)
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011716 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0011716 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0011716 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for spdo Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive spdoC55 spdoK433 spdoS097002b lethal | embryonic stage spdoG104 spdoH7 spdoK433 spdounspecified lethal | embryonic stage | recessive spdoS097002b Other Phenotypes Allele neuroanatomy defective spdoC55 spdoG104 Phenotype manifest in Allele cardioblast spdoC55 thecogen cell & peripheral nervous system spdoC55 spdoK433 embryonic peripheral nervous system spdoC55 embryonic peripheral nervous system & glial cell spdoC55 spdoH7 spdounspecified CNS glial cell spdoC55 spdoG104 spdoK433 longitudinal connective spdoC55 spdoG104 spdoK433 spdounspecified anterior commissure spdoC55 spdoG104 spdoK433 posterior commissure spdoC55 spdoG104 spdoK433 abdominal dorsal bipolar neuron dbp | ectopic spdoC55 dorsal multidendritic neuron | ectopic spdoC55 macrochaeta | somatic clone spdoC55 eo neuron | somatic clone spdoC55 macrochaeta & mesothoracic tergum | somatic clone spdoG104 embryonic central nervous system spdoG104 spdoS097002b spdounspecified GW neuron spdoG104 EW1 neuron | ectopic spdoG104 peripheral nervous system spdoG104 spdoK433 external sensory organ precursor cell IIb spdoG104 spdoK433 thecogen cell spdoG104 spdoK433 multidendritic neuron spdoG104 spdoK433 multidendritic neuron | precursor spdoG104 spdoK433 abdominal 1 basiconical sensillum dbd spdoG104 spdoK433 abdominal 2 basiconical sensillum dbd spdoG104 spdoK433 abdominal 3 basiconical sensillum dbd spdoG104 spdoK433 abdominal 4 basiconical sensillum dbd spdoG104 spdoK433 abdominal 5 basiconical sensillum dbd spdoG104 spdoK433 abdominal 6 basiconical sensillum dbd spdoG104 spdoK433 abdominal 7 basiconical sensillum dbd spdoG104 spdoK433 abdominal 1 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 2 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 3 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 4 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 5 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 6 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 7 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 external sensory organ spdoG104 spdoK433 RP2 neuron spdoG104 RP2sib neuron spdoG104 aCC neuron spdoG104 pCC neuron spdoG104 dMP2 neuron spdoG104 vMP2 neuron spdoG104 U neuron spdoG104 spdodsRNA.cOa Usib neuron spdoG104 peripheral nervous system precursor cluster spdoH7 spdounspecified peripheral nervous system precursor cluster & neuron | supernumerary spdoH7 spdounspecified abdominal 1 dorsal oblique muscle 5 spdoK433 abdominal 2 dorsal oblique muscle 5 spdoK433 abdominal 3 dorsal oblique muscle 5 spdoK433 abdominal 4 dorsal oblique muscle 5 spdoK433 abdominal 5 dorsal oblique muscle 5 spdoK433 abdominal 6 dorsal oblique muscle 5 spdoK433 abdominal 7 dorsal oblique muscle 5 spdoK433 abdominal 1 dorsal acute muscle 3 spdoK433 abdominal 2 dorsal acute muscle 3 spdoK433 abdominal 3 dorsal acute muscle 3 spdoK433 abdominal 4 dorsal acute muscle 3 spdoK433 abdominal 5 dorsal acute muscle 3 spdoK433 abdominal 6 dorsal acute muscle 3 spdoK433 abdominal 7 dorsal acute muscle 3 spdoK433 embryonic/larval pericardial cell spdoK433 neuron & embryo spdoS097002b RP2 neuron | supernumerary spdodsRNA.cOa abdominal dorsal bipolar neuron dbp & embryo | supernumerary spdounspecified abdominal dorsal bipolar neuron dbp & embryo | precursor spdounspecified
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of spdo Class Mutagen Stocks Known lesion spdoAB153 0 Yes spdoAC81 0 Yes spdoAC85 0 Yes spdoC55 loss of function ethyl methanesulfonate 1 Yes spdoDG23207 0 -- spdoG104 loss of function ethyl methanesulfonate 1 Yes spdoH7 loss of function P-element activity 0 Yes spdoK433 loss of function ethyl methanesulfonate 0 Yes spdoOO3 0 Yes spdoP46 0 Yes spdoS097002b Δ2-3 P-element activity 0 Yes spdoVV86 0 Yes spdoYY233 0 -- spdoZ143 0 Yes spdoZZ213 0 Yes spdounspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spdo Class Mutagen Stocks Known lesion spdoGD3130 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes spdoScer\UAS.cOa in vitro construct | regulatory fusion 1 Yes spdodsRNA.cOa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)tll-g
Useful duplication	Dp(3;3)516
Disrupted in	Df(3R)Exel6215 (Ryder, 2004.4.26) Df(3R)Exel6216 (Ryder, 2004.4.26, Abrams et al., 2006) Df(3R)ZZ27 (Dye et al., 1998, Park et al., 1998, Skeath, 2004) Df(3R)tll-g (Dye et al., 1998, Salzberg et al., 1994) T(Y;3)R133 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3130} NA P{UAS-spdo.O} NA heat-shock construct P{HS-tmod.E42} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}tmodc01634 NA PBac{PB}tmodc01645 NA PBac{PB}tmodc03546 NA PBac{PB}tmodc06280 NA PBac{RB}tmode00860 NA PBac{WH}tmodf00674 NA PBac{WH}tmodf03538 NA PBac{WH}tmodf04997 NA PBac{WH}tmodf05684 NA PBac{WH}tmodf06991 NA insertion of enhancer trap P{PZ}tmod02288 No P{lacW}tmodH7 Yes
Related Comments
	Please look at the allele reports for the complete phenotype data Mutation in spdo affects the neuronal lineage, causes transformation of support cells into neurons. (Salzberg et al., 1994) spdo is required to define glial versus neuronal cell identity. (Lee et al., 1995) spdo is required for PNS development in the embryo. (Salzberg et al., 1994) Mutations in spdo affect the asymmetric cell division of the embryonic PNS SOPIIb. Rather than a neuron and a glial cell, two neurons are produced. spdo is also required in several other PNS lineages. spdo is epistatic to numb. (Dye et al., 1998) The numb mutant phenotype is extremely sensitive to the dosage of spdo. (Skeath and Doe, 1998) Two EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999)
Gene Ontology: Function, Process & Cellular Component ( 11 )
Molecular Function
CV term Evidence References
Notch binding inferred from physical interaction with FLYBASE:N; FB:FBgn0004647 (O'Connor-Giles and Skeath, 2003)
protein binding inferred from physical interaction with FLYBASE:numb; FB:FBgn0002973 (O'Connor-Giles and Skeath, 2003)
Biological Process
CV term Evidence References
Notch signaling pathway inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647 AND inferred from physical interaction with FLYBASE:N; FB:FBgn0004647 (O'Connor-Giles and Skeath, 2003)
asymmetric cell division inferred from mutant phenotype (O'Connor-Giles and Skeath, 2003)
peripheral nervous system development traceable author statement (Salzberg et al., 1994) non-traceable author statement (Prokopenko et al., 2000)
sensory organ development inferred from mutant phenotype (Roegiers et al., 2005)
Cellular Component
CV term Evidence References
cortical cytoskeleton inferred from direct assay (Roegiers et al., 2005)
cytoplasm inferred from direct assay (O'Connor-Giles and Skeath, 2003)
colocalizes_with cytoplasmic vesicle inferred from direct assay (Jafar-Nejad et al., 2005)
integral to membrane inferred from sequence or structural similarity (O'Connor-Giles and Skeath, 2003)
plasma membrane inferred from direct assay (O'Connor-Giles and Skeath, 2003) inferred from direct assay (Roegiers et al., 2005)
colocalizes_with plasma membrane inferred from direct assay (Jafar-Nejad et al., 2005)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spdo allele Gene References spdoG104 numb (Lundell et al., 2003) spdoH7 numb (Dye et al., 1998) tmod (Dye et al., 1998) unspecified numb (Skeath and Doe, 1998)
External Data
Linkouts	Drosophila PIMRider - The Drosophila Protein Interaction map • 537603
Orthologs
Genome-wide drosophilid orthologs	Dgri\GH14100 Dmoj\GI24859 Dper\GL13461 Dvir\GJ24501 Dyak\GE10880
Curated drosophilid orthologs	Dpse\GA13696   Dpse\GA15944
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Harvard	- PBac{PB}tmodc01645 - PBac{PB}tmodc03546 - PBac{PB}tmodc06280 - PBac{RB}tmode00860 - PBac{WH}tmodf00674 - PBac{WH}tmodf03538 - PBac{WH}tmodf04997 - PBac{WH}tmodf05684
VDRC	v6858 w1118 P{GD3130}v6858 v6856 w1118; P{GD3130}v6856/TM3
Bloomington	9934 w*; P{UAS-spdo.O}G14