Gene Dmel\Snr1

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General Information

Symbol

Dmel\Snr1

Species

D. melanogaster

Name

Snf5-related 1

Annotation symbol

CG1064

Feature type

protein_coding_gene

FlyBase ID

FBgn0011715

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

83A4-83A4

Sequence location

3R:1,296,639..1,298,481 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Snf5-related 1 is referred to in FlyBase by the symbol Snr1 (CG1064, FBgn0011715). It has the cytological map location 83A4. Its sequence location is 3R:1296639..1298481. Its molecular function is described as: RNA polymerase II transcription factor activity; transcription coactivator activity; general RNA polymerase II transcription factor activity. It is involved in the biological processes: regulation of transcription; positive regulation of transcription, DNA-dependent; embryonic development via the syncytial blastoderm; instar larval or pupal development; imaginal disc-derived wing vein specification; muscle development; dendrite morphogenesis; epidermis development; chromatin remodeling. 20 alleles are reported. The phenotypes of these alleles are annotated with: abdominal tergite; wing; wing vein; adult cuticle; eye; wing vein L2; wing sensillum. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83A4-83A4

Limits computationally determined from genome sequence between P{EP}EP974 and P{PZ}Snr1⁰¹³¹⁹&P{PZ}Itp-r83A⁰⁵⁶¹⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

83A5-83A6

(Dingwall, 1995.6.6)

83A5-83A6

(determined by in situ hybridisation)

(Spradling et al., 1999)

83A5-83A6

(determined by in situ hybridisation)

(Dingwall et al., 1995)

83A5-83A6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

(Dingwall, 1995.6.6)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Snr1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Snr1-RA

FBtr0078682

NM_169055

1349

370

Additional Transcript Data & Comments

Reported size (kB)

1.4 (northern blot)

(Dingwall et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Snr1-PA

FBpp0078331

41.9

370

4.91

NP_730935

AAF52024

Additional Polypeptide Data & Comments

Reported size (kD)

370 (aa); 43 (kD observed); 43 (kD predicted)

(Dingwall et al., 1995)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011715

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SNF5/SMARCB1/INI1 (IPR006939)

SWI/SNF chromatin-remodeling complex, component hSNF5/Ini1 (IPR017393)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24090

Maps to

Does NOT map to

Identified with

GH08712

(BDGP Project Members, 2000-)

LD18257

(Spradling et al., 1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

Snr1[R3]
3R:1,297,665..1,297,667

comment=Imprecise excision of P element results in an in-frame stop codon at amino acid 131.
evidence=experimental

(Dingwall et al., 1995)

deletion

Snr1[R10]
3R:1,297,665..1,297,667

comment=Imprecise excision of P element results in an in-frame stop codon at amino acid 131.
evidence=experimental

(Dingwall et al., 1995)

rescue fragment

Snr1[+t8.9]
3R:1,290,932..1,299,838

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=EcoRI-EcoRI_rfrag

(Zraly et al., 2003)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6262

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-pupal stage

(Dingwall et al., 1995)

adult stage | female

(Dingwall et al., 1995)

embryonic stage-adult stage

(Zraly et al., 2003)

unfertilized egg

(Dingwall et al., 1995)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | early

(Dingwall et al., 1995)

embryonic stage | stage >=13

embryonic brain

(Dingwall et al., 1995)

larval stage | third instar

larval optic lobe | restricted

(Zraly et al., 2003)

larval stage

imaginal disc

(Dingwall et al., 1995)

adult stage | female

(Dingwall et al., 1995)

larval stage

embryonic/larval salivary gland

(Dingwall et al., 1995)

larval stage | third instar

dorsal mesothoracic disc | restricted

(Zraly et al., 2003)

larval stage | third instar

larval salivary gland

(Zraly et al., 2003)

embryonic stage | stage >=13

embryonic central nervous system

(Dingwall et al., 1995)

larval stage | third instar

eye disc

(Zraly et al., 2003)

embryonic stage-pupal stage

(Dingwall et al., 1995)

larval stage | third instar

germarium region 2 & germarium region 3

(Zraly et al., 2003)

larval stage | third instar

ventral thoracic disc | restricted

(Zraly et al., 2003)

Marker for

Subcellular Localization

CV Term

nucleus

(Dingwall et al., 1995)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI109494

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011715

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011715

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011715

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Snr1

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Snr1

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Snr1^P1rev

lethal | recessive

lethal | conditional - heat sensitive, with Scer\GAL4^e22c, Snr1^R3

Snr1^{cdel.3.Scer\UAS}

lethal | conditional - heat sensitive, with Scer\GAL4^e22c, Snr1^{cdel.3.Scer\UAS}

Snr1^R3

lethal | larval stage | recessive

lethal, with Scer\GAL4^hs.PB

Snr1^{cdel.3.Scer\UAS}

cell lethal | recessive | somatic clone

Snr1^R3

Other Phenotypes

Allele

visible (with Snr1^R3)

Snr1^E1

visible (with Snr1^SR21)

Snr1^E1

visible (with Snr1^E1)

visible | recessive | somatic clone

Snr1^R3

visible | dominant

Snr1^E1

visible, with Scer\GAL4^69B

Snr1^{cdel.3.Scer\UAS}

visible, with Scer\GAL4^e22c

Snr1^{cdel.3.Scer\UAS}

body color defective | somatic clone

Snr1^R3

large body

Snr1^E1

increased cell size

Snr1^dsRNA.cZa

non-enhancer of variegation, with In(1)w^m4h

Snr1^P1rev

suppressor of variegation | dominant, with In(1)w^m4h

non-suppressor of variegation, with In(1)w^m4h

Snr1^P1rev

short lived, with Scer\GAL4^Act5C.PI, Snr1^R3

Snr1^{cdel.3.Scer\UAS}

short lived, with Scer\GAL4^Act5C.PI, Snr1^{cdel.3.Scer\UAS}

Snr1^R3

Sterility

Allele

female sterile | germ-line clone

Snr1^R3

female sterile, with Scer\GAL4^Act5C.PI, Snr1^R3

Snr1^{cdel.3.Scer\UAS}

female sterile, with Scer\GAL4^Act5C.PI, Snr1^{cdel.3.Scer\UAS}

Snr1^R3

Phenotype manifest in

Allele

wing (with Snr1^R3)

Snr1^E1

wing vein | ectopic (with Snr1^R3)

Snr1^E1

wing vein (with Snr1^SR21)

Snr1^E1

wing vein | ectopic

Snr1^E1

wing

Snr1^E1

abdominal tergite, with Scer\GAL4^e22c, Snr1^{cdel.3.Scer\UAS}

Snr1^R3

wing (with Snr1^E1)

Snr1^R3

wing vein | ectopic (with Snr1^E1)

Snr1^R3

mechanosensory chaeta & adult abdomen | somatic clone

Snr1^R3

mechanosensory chaeta | supernumerary & adult abdomen | somatic clone

Snr1^R3

socket & adult abdomen | somatic clone

Snr1^R3

adult cuticle | somatic clone

Snr1^R3

eye | somatic clone

Snr1^R3

adult thorax | dorsal & chaeta | somatic clone

Snr1^R3

wing vein (with Snr1^E1)

Snr1^SR21

abdominal tergite, with Scer\GAL4^e22c, Snr1^R3

Snr1^{cdel.3.Scer\UAS}

abdominal tergite, with Scer\GAL4^e22c

Snr1^{cdel.3.Scer\UAS}

wing vein L2, with Scer\GAL4^69B

Snr1^{cdel.3.Scer\UAS}

wing sensillum | ectopic, with Scer\GAL4^69B

Snr1^{cdel.3.Scer\UAS}

Classical Alleles ( 11 )

For All Classical Alleles Show

Allele of Snr1	Class	Mutagen	Stocks	Known lesion
Snr1⁰¹³¹⁹		P-element activity	1	Yes
Snr1⁰³¹⁹			0	--
Snr1^E1		ethyl methanesulfonate	0	Yes
Snr1^E2		ethyl methanesulfonate	0	Yes
Snr1^E3		ethyl methanesulfonate	0	Yes
Snr1^E4		ethyl methanesulfonate	0	--
Snr1^P1rev		Δ2-3	0	--
Snr1^R10		Δ2-3	0	Yes
Snr1^R3		Δ2-3	0	Yes
Snr1^SR21	loss of function	P-element activity	0	Yes
Snr1^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Snr1	Mutagen	Stocks	Known lesion
Snr1^+t8.9	in vitro construct \| genomic fragment	0	Yes
Snr1^3.Scer\UAS	in vitro construct \| regulatory fusion	0	Yes
Snr1^4.Scer\UAS	in vitro construct \| regulatory fusion	0	Yes
Snr1^GD4140	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Snr1^Scer\UAS.cZa	in vitro construct \| regulatory fusion	0	Yes
Snr1^{cdel.3.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| deletion	0	Yes
Snr1^dsRNA.cZa	in vitro construct \| RNAi	0	Yes
Snr1^hs.PZ	in vitro construct \| regulatory fusion	0	Yes
Snr1^{hs.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful duplication

Dp(3;3)Tpl-T34

Not disrupted in

Df(3R)2-2

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Disrupted in

Df(3R)SR29

(Zraly et al., 2003)

Df(3R)SR71

(Zraly et al., 2003)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{Snr1^+t8.9}

heat-shock construct

P{HA-hs-Snr1}

P{hs-Snr1.Z}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{PZ}Snr1⁰¹³¹⁹

Yes

Related Comments

Please look at the allele reports for the complete phenotype data

The Snr1 and brm proteins are present in a large complex and co-precipitate from extracts, these results suggest that the Drosophila counterpart of the yeast SWI/SNF complex plays an important role in counteracting the repressive effects of chromatin on homeotic gene transcription during development.

(Dingwall et al., 1995)

Snr1 is essential for viability. Phenotypic studies and genetic interactions suggest that Snr1 and brm act together, and with trx, to regulate homeotic gene transcription. Snr1 and brm proteins are present in a large complex, this complex may play an important role in maintaining homeotic gene transcription during development by counteracting the repressive effect of chromatin.

(Dingwall et al., 1995)

Loss of Snr1 function results in ectopic vein material in the wing.

(Marenda et al., 2004)

dsRNA has been made from templates generated with primers directed against this gene. RNAi of Snr1 results in dorsal overextension of primary dendrites and a reduction in lateral branching. RNAi also causes defects in muscle, defects in the epidermis, defects in dendrite morphogenesis and reproducible defects in da dendrite development.

(Parrish et al., 2006)

Gene Ontology: Function, Process & Cellular Component ( 15 )

Molecular Function

CV term

Evidence

References

RNA polymerase II transcription factor activity

non-traceable author statement

(Dingwall, 1995.6.6)

general RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with SGD_LOCUS:SNF5; SGD:S0000493

(FlyBase, 1992-)