Gene Dmel\Sep1

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General Information

Symbol

Dmel\Sep1

Species

D. melanogaster

Name

Septin-1

Annotation symbol

CG1403

Feature type

protein_coding_gene

FlyBase ID

FBgn0011710

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

19F5-19F5

Sequence location

X:21,229,303..21,232,714 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Septin-1 is referred to in FlyBase by the symbol Sep1 (CG1403, FBgn0011710). It has the cytological map location 19F5. Its sequence location is X:21229303..21232714. Its molecular function is described as: GTP binding; GTPase activity; protein binding. It is involved in the biological processes: cytokinesis; cellularization; cell cycle. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19F5-19F5

Limits computationally determined from genome sequence between P{EP}CG1702^EP1525 and P{EP}EP1465&P{EP}CG1486^EP1192

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

19F4-19F5

(determined by in situ hybridisation)

(Fares et al., 1995)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: Sep1+ waw+ slgA+ In direction of increasing cytology: Sep1+ bbx+ slgA+

(Hayward et al., 1993)

In direction of increasing cytology: tty? fliI? dod+ pen- dod+ pen- sol? Sep1? waw? bbx? slgA? Hlc? mst? lcs?

(Maleszka et al., 1998)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: tty- fliI+ dod+ pen- sol+ Sep1+ waw+ bbx+ slgA+ Hlc- mst+ lcs-

(Miklos, 1997.8.4)

Gene Model & Products

Please see the GBrowse view of Dmel\Sep1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sep1-RA

FBtr0077198

NM_078706

2035

361

Sep1-RB

FBtr0077199

NM_167747

643

165

Additional Transcript Data & Comments

Reported size (kB)

2.1 (northern blot)

(Hayward et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sep1-PA

FBpp0076897

41.1

361

6.52

NP_523430

AAF50825

Sep1-PB

FBpp0076898

18.6

165

5.29

NP_728418

AAN09677

Additional Polypeptide Data & Comments

Reported size (kD)

361 (aa)

(Maleszka et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011710

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cell division/GTP binding protein (IPR000038)

Septin 2 (IPR008113)

Septin (IPR016491)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P42207

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE30523

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5184

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

(Maleszka et al., 1998)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011710

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011710

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011710

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for Sep1

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Sep1

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Sep1	Class	Mutagen	Stocks	Known lesion
Sep1¹			0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sep1	Class	Mutagen	Stocks	Known lesion
Sep1^GD8198		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Sep1^GL1		in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)17-257

Df(1)2/19B

Useful duplication

Dp(1;Y)y⁺mal¹⁷¹

Disrupted in

Df(1)2/19B

(Hayward et al., 1993)

Not disrupted in

Df(1)JC77

(Hayward et al., 1993)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD8198}

characterization construct

P{pGL1}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Sep1 localises to the leading edge of the cleavage furrows in dividing cells and in cellularising embryos. Sep1 is also concentrated in other locations that suggest additional roles unrelated to cytokinesis. Immunolocalisation and biochemical data suggest that Sep1 and pnut probably function as parts of a complex.

(Fares et al., 1995)

A complex of septin polypeptides is isolated that bind and hydrolyse GTP and form filaments. Their high degree of conservation, ubiquitous expression and proven role in cytokinesis suggests septins are certain to be important players in regulating cell architecture and function. Septins alone can form regular filamentous polymers, since filament formation is likely to be central to their localisation and function.

(Field et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

GTP binding

inferred from sequence or structural similarity with InterPro:IPR000038

(FlyBase, 1992-)

GTPase activity

non-traceable author statement

(Hales et al., 1999)

protein binding

inferred from physical interaction with FLYBASE:sip2; FB:FBgn0031878

(Shih et al., 2002)

Biological Process

CV term

Evidence

References

cell cycle

inferred from electronic annotation with InterPro:IPR000038, InterPro:IPR008113

(FlyBase Curators et al., 2004-)

cellularization

traceable author statement

(Mazumdar and Mazumdar, 2002)

cytokinesis

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1995.6.1)

Cellular Component

CV term

Evidence

References

germline ring canal

non-traceable author statement

(Deng and Lin, 2001)

septin complex

inferred from electronic annotation with InterPro:IPR016491

(FlyBase Curators et al., 2004-)

septin ring

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Sep1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59391

Orthologs

Genome-wide drosophilid orthologs

Dana\GF20920

Dere\GG19725

Dgri\GH24812

Dmoj\GI21539

Dper\GL16012

Dpse\GA12712

Dsec\GM23080

Dvir\GJ16012

Dyak\GE17923

Curated drosophilid orthologs

Dpse\GA12712

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000021002
Anopheles gambiae (Ensembl)
ENSBTAP00000003376
Bos taurus (Ensembl)
ENSCAFP00000019094
Canis familiaris (Ensembl)
ENSCINP00000018050
Ciona intestinalis (Ensembl)
NEWSINFRUP00000170756
Takifugu rubripes (Ensembl)
ENSGALP00000009314
Gallus gallus (Ensembl)
ENSP00000353157
Homo sapiens (Ensembl)
ENSMMUP00000006294
Macaca mulatta (Ensembl)
ENSMODP00000020021
Monodelphis domestica (Ensembl)
ENSPTRP00000041654
Pan troglodytes (Ensembl)
ENSRNOP00000024261
Rattus norvegicus (Ensembl)
GSTENP00015007001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000026555
Xenopus tropicalis (Ensembl)
ZDB-GENE-030131-911
Danio rerio (Zfin)
MGI:97298
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v17344

w¹¹¹⁸; P{GD8198}v17344

Genomic Clones ( 2 )

cDNA Clones ( 82 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE30523

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones