Gene Dmel\fzo

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: cnc+ fzo+ cnc+

(McGinnis et al., 1998)

Gene Model & Products

Please see the GBrowse view of Dmel\fzo for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

fzo-RA

FBtr0084399

NM_170060

2399

718

Additional Transcript Data & Comments

Reported size (kB)

2.399 (longest cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

fzo-PA

FBpp0083791

81.6

718

6.06

NP_732840

AAF56110

Additional Polypeptide Data & Comments

Reported size (kD)

718 (aa)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011596

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Dynamin, GTPase region (IPR001401)

Fzo-like conserved region (IPR006884)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O18412

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

rescue fragment

fzo[KH3]
3R:19,026,803..19,030,823

comment=position of restriction fragment on reference sequence inferred by FlyBase curator
evidence=experimental
linked_to=EcoRI-XhoI_rfrag

External Data

DEDB - Drosophila exon database: splicing graphs

•

10429

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

testis

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

spermatogenesis,adult stage

Nebenkern

spermatogenesis,adult stage

spermatid

Marker for

Subcellular Localization

CV Term

mitochondrial membrane

External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011596

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011596

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011596

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Sterility

Allele

male sterile | recessive

female fertile

Phenotype manifest in

Allele

spermatid (with fzo²)

fzo¹

Nebenkern

spermatid & mitochondrion

onion stage spermatid & mitochondrion

Nebenkern | germ-line clone

fzo¹

spermatid (with fzo¹)

fzo²

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of fzo	Class	Mutagen	Known lesion
fzo¹	loss of function	ethyl methanesulfonate	--
fzo²	loss of function	ethyl methanesulfonate	--
fzo^GS8035			Yes
fzo^z3-4436			--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of fzo	Mutagen	Stocks	Known lesion
fzo^GD196	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
fzo^K99T	in vitro construct \| amino acid replacement in vitro construct \| site directed mutagenesis	0	Yes
fzo^KH3	in vitro construct \| genomic fragment	0	Yes
fzo^R249L	in vitro construct \| amino acid replacement in vitro construct \| site directed mutagenesis	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)M95A

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)Exel6274

(Ryder, 2004.4.26)

Df(3R)Exel6280

(Ryder, 2004.4.26)

Df(3R)M95A

Df(3R)P2O

Not disrupted in

Df(3R)EB6

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD196}

characterization construct

P{KH3fzo⁺}

P{fzo^K99T}

P{fzo^R249L}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{GSV3}cnc^GS8035

Related Comments

Please look at the allele reports for the complete phenotype data

At the round spermatid stage all mitochondria in each haploid spermatid fuse into two large derivatives that interweave to form the nebenkern, in fzo mutants the mitochondria aggregate but fail to fuse. The defect is specific to fusion as each unfused mitochondrion elongates normally.

(Hales and Fuller, 1995)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

GTP binding

inferred from electronic annotation with InterPro:IPR001401

GTPase activity

inferred from sequence or structural similarity

non-traceable author statement

(Hales, 1997.3.30)

inferred from electronic annotation with InterPro:IPR001401, InterPro:IPR006884

Biological Process

CV term

Evidence

References

Nebenkern formation

inferred from mutant phenotype

traceable author statement

(Fuller, 1998)

mitochondrial fusion

inferred from mutant phenotype

mitochondrion organization and biogenesis

traceable author statement

(Mozdy and Shaw, 2003)

inferred from mutant phenotype

(Wilson and Hales, 2002)

spermatogenesis

traceable author statement

(Mozdy and Shaw, 2003)

Cellular Component

CV term

Evidence

References

integral to membrane

traceable author statement

(Mozdy and Shaw, 2003)

inferred from electronic annotation with InterPro:IPR006884

mitochondrial envelope

inferred from direct assay

mitochondrial outer membrane

inferred from electronic annotation with InterPro:IPR006884

plasma membrane

non-traceable author statement

(Hales, 1997.3.30)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

fzo allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

67689

Orthologs

Genome-wide drosophilid orthologs

Dper\GL23042

Curated drosophilid orthologs

Dpse\GA18264

Dsim\fzo

(Begun, 2000.4.5)

InParanoid orthologs - Eukaryotic orthologs

•

SPBC1706.03
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v1407

w¹¹¹⁸; P{GD196}v1407

v1405

w¹¹¹⁸; P{GD196}v1405/TM3

Genomic Clones ( 1 )

BACR03N12

cDNA Clones ( 4 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

IP03008

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0011596

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0011596

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: fzo anon-WO0125274.1 anon-WO0125274.25 anon-WO0125274.26 Source for merge of fzo anon-WO0125274.1 anon-WO0125274.25 anon-WO0125274.26 was sequence comparison (date:051113).

(FlyBase, 1996-)

Additional comments

Comments About Role

fzo has been cloned and characterised. fzo encodes a predicted transmembrane GTPase that is required for mitochondrial fusion during spermatogenesis.