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Gene Dmel\Acp36DE

General Information
Symbol	Dmel\Acp36DE	Species	D. melanogaster
Name	Accessory gland peptide 36DE	Annotation symbol	CG7157
Feature type	protein_coding_gene	FlyBase ID	FBgn0011559
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	36F3-36F3	Sequence location	2L:18,356,113..18,359,026 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Accessory gland peptide 36DE is referred to in FlyBase by the symbol Acp36DE (CG7157, FBgn0011559). It has the cytological map location 36F3. Its sequence location is 2L:18356113..18359026. Its molecular function is described as hormone activity. It is involved in the biological processes: negative regulation of female receptivity, post-mating; sperm storage; sperm displacement; mating plug formation; sperm competition. 10 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 36F3-36F3   Limits computationally determined from genome sequence between P{lacW}Aac11k06710 and P{EP}CG10413EP2164
Experimentally Determined Cytological Location
Cytogenetic map Notes References 36D-36E   (Wolfner, 1999.6.8) 36D-36E (determined by in situ hybridisation)   (Wolfner et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Acp36DE for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Acp36DE-RA FBtr0081054  NM_078870   2855   912
Additional Transcript Data & Comments
Reported size (kB)	2.7 (northern blot) (Wolfner et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Acp36DE-PA   FBpp0080607   101.9   912   9.57     NP_523594   AAF53664
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003658 AAF53664   AF145666 AAD38641   AF157488 AAD40185   AI109272   AI133970   AI135551   AI135623   AI387036   AI388549   AI389566   AI402256   AI403827   AX154706   AY010577 AAG37359   AY010578 AAG37360   AY010579 AAG37361   AY010580 AAG37362   AY010581 AAG37363   AY010582 AAG37364   AY010583 AAG37365   AY010584 AAG37366   AY010585 AAG37367   AY010586 AAG37368   AY010587 AAG37369   AY010588 AAG37370   AY010589 AAG37371   AY010590 AAG37372   AY010591 AAG37373   AY010592 AAG37374   AY113344 AAM29349   BF486497   BF487284   BF487933   BF488721   BF488899   BF491252   BF493256   BF495396   BF496078   BF496626   BF496967   BF497975   BF498078   BF498701   BF498788   BF498841   BF499193   BF500090   BF501152   BF501475   BF501483   BF502266   BF502298   BF503147   BF503492   BF503774   BF504225   BF504824   BF505554   BF505774   BF506151   BG634695   U85759 AAB96383
UniProtKB/Swiss-Prot	Q9V3R1
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	GH13354 (BDGP Project Members, 2000-) GH11288 (Rubin, 1999.4.23, BDGP Project Members, 2000-, Zou et al., 2000)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7959
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage | male  male accessory gland main cell (Wolfner et al., 1997)  adult stage | male  male accessory gland (Wolfner et al., 1997)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI133970
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011559 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0011559 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0011559
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Acp36DEN1 Sterility Allele male sterile | partially | recessive Acp36DEN1 male fertile Acp36DEP Phenotype manifest in Allele
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Acp36DE Class Mutagen Stocks Known lesion Acp36DE1 0 -- Acp36DE2 0 -- Acp36DE3 0 -- Acp36DE4 0 -- Acp36DEM36F-2 0 -- Acp36DEM36F-S6 0 -- Acp36DEN1 ethyl methanesulfonate 0 -- Acp36DEN2 ethyl methanesulfonate 0 -- Acp36DEP P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Acp36DE Class Mutagen Stocks Known lesion Acp36DEGD5318 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)M36F-S5
Disrupted in	Df(2L)Exel8038 (Ryder, 2004.4.26) Df(2L)H20 (Marygold, 2005.1.19)
Partially disrupted in	Df(2L)M36F-2 (Marygold, 2005.1.19) Df(2L)M36F-S6 (Marygold, 2005.1.19)
Not disrupted in	Df(2L)TW50 (Marygold, 2005.1.19)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5318} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{}Acp36DEP NA insertion of enhancer trap P{lacW}l(2)SH0133SH0133 No
Related Comments
	Please look at the allele reports for the complete phenotype data Acp36DE seminal fluid protein is localised in the mated female suggesting a function of the protein in restricting sperm to an area in the mated female's genital tract (just above the openings of the sperm storage organs) that facilitates sperm storage. (Bertram et al., 1996) Males null for Acp36DE do poorly in fertilisations as second-mating males because fewer sperm are stored or retained in mated females, not because Acp36DE protein itself displaces sperm. (Chapman et al., 2000)
Gene Ontology: Function, Process & Cellular Component ( 7 )
Molecular Function
CV term Evidence References
hormone activity non-traceable author statement (Wolfner et al., 1997) non-traceable author statement (FlyBase, 1992-)
Biological Process
CV term Evidence References
mating plug formation traceable author statement (Chapman, 2001)
negative regulation of female receptivity, post-mating non-traceable author statement (Wolfner et al., 1997)
sperm competition traceable author statement (Chapman, 2001)
sperm displacement non-traceable author statement (FlyBase, 1992-)
sperm storage inferred from mutant phenotype (Chapman et al., 2000) traceable author statement (Karr and Pitnick, 1999) traceable author statement (Chapman, 2001) traceable author statement (Billeter et al., 2002) traceable author statement (Wolfner, 2002) traceable author statement (Qazi et al., 2003)
Cellular Component
CV term Evidence References
extracellular region traceable author statement (Wolfner et al., 1997) non-traceable author statement (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Acp36DE allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61091
Orthologs
Genome-wide drosophilid orthologs	Dere\GG16558 Dsec\Acp36DE Dsim\Acp36DE Dyak\GE18098
Curated drosophilid orthologs	Dmau\Acp36DE (Jones et al., 2003) Dsec\Acp36DE (Jones et al., 2003) Dsim\Acp36DE (Snook et al., 2000, Swanson et al., 2001) Dyak\Acp36DE (Jones et al., 2003)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • WBGene00037178 Caenorhabditis briggsae (Wormbase)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Szeged	- P{lacW}l(2)SH0133SH0133
VDRC	v37992 w1118; P{GD5318}v37992/TM3 v37993 w1118; P{GD5318}v37993/TM3
Genomic Clones ( 3 )
	BACR01A03 BACR04D05 BACR05N10
cDNA Clones ( 34 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH11288 GH13354
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12319 AT10511 AT01571 AT05543 AT20818 AT24331 AT19061 GH08411 AT10989 AT16828 GH13444 GH17453 AT16391 AT09878 AT13394 AT24574 AT31002 AT12438 AT16838 GH19492 AT07803 AT06855 AT08153 AT08549 AT27953 AT04974 AT13156 AT23061 GH23349 AT17939 GH20778 AT19487
Other clones
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0011559 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0011559
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Acp36DE anon-WO0140519.55 Source for merge of Acp36DE anon-WO0140519.55 was sequence comparison (date:051113). (FlyBase, 1996-)
Additional comments	"Acp36DE" may correspond to "AcpC", unfortunately strains of "AcpC" are no longer extant. (Wolfner et al., 1997)
Comments About Role
	May promote replacement of sperm already stored by a female.   Screening of natural populations for P-element tagged genes has identified an insert within 2kb of Acp36DE. (Clark et al., 1994) If females remate frequently relative to the duration of sperm storage and rate of sperm use, sperm displacement may be an important component of male reproductive success. Significant association has been found between Acp36DE mutant male sperm and the ability to resist displacement by subsequent sperm. There is no correlation between the ability to displace resident sperm and the ability to resist being displaced by subsequent sperm, this lack of correlation suggests that different mechanisms mediate that the two components of sperm displacement. (Clark et al., 1995) Acp36DE gene product is a seminal fluid protein whose fate after mating is consistent with a role in sperm storage. (Neubaum et al., 1995) Male derived Acp36DE protein is essential for efficient sperm storage by females; it is essential for the initial storage of sperm and may also influence the arrangement and retention of stored sperm. (Neubaum and Wolfner, 1999) Not required for viability. (Marygold, 2005.1.19)
Comments About Molecular Function
Other Comments
	Gene is identified in a differential cDNA hybridisation screen. (Wolfner et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078870 RefSeq (Proteins) • NP_523594 DDBJ / EMBL / GenBank • AE003658 AAF53664 AF145666 AAD38641 AF157488 AAD40185 AI133970 AI135551 AI402256 AX154706 AY010577 AAG37359 AY010578 AAG37360 AY010579 AAG37361 AY010580 AAG37362 AY010581 AAG37363 AY010582 AAG37364 AY010583 AAG37365 AY010584 AAG37366 AY010585 AAG37367 AY010586 AAG37368 AY010587 AAG37369 AY010588 AAG37370 AY010589 AAG37371 AY010590 AAG37372 AY010591 AAG37373 AY010592 AAG37374 AY113344 AAM29349 U85759 AAB96383 UniProtKB/Swiss-Prot • Q9V3R1
Other Crossreferences
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61091 DEDB - Drosophila exon database: splicing graphs • 7959 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0011559 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011559 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0011559 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0011559 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0011559 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0011559 InParanoid orthologs - Eukaryotic orthologs • WBGene00037178 Caenorhabditis briggsae (Wormbase) University of Chicago Dev. Expression • FBgn0011559
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	Acp36DE (Ravi Ram et al., 2005, Avila, 2008) anon-WO0140519.55   CG7157 (Arbeitman et al., 2004, Labourier et al., 2002, ) CT22109 (Ceriani et al., 2002)
Name Synonym	36DE accessory gland protein (Zou et al., 2000, Wolfner, 1999.6.8) Accessory gland peptide 36DE   Accessory gland protein (Wolfner et al., 1997)
Secondary FlyBase IDs
	FBgn0044574
References ( 57 )
Generate a list of	All references relating to this gene ( 57 )
List References by type