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Gene Dmel\Snap25

General Information
Symbol	Dmel\Snap25	Species	D. melanogaster
Name	Synapse protein 25	Annotation symbol	CG40452
Feature type	protein_coding_gene	FlyBase ID	FBgn0011288
Created / Updated	2006-06-13/2006-06-13
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map		Sequence location	3L:24,067,329..24,292,305 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Synapse protein 25 is referred to in FlyBase by the symbol Snap25 (CG40452, FBgn0011288). Its sequence location is 3L:24067329..24292305. Its molecular function is described as: soluble NSF attachment protein activity; SNAP receptor activity. It is involved in the biological processes: neurotransmitter secretion; vesicle-mediated transport; Golgi to plasma membrane transport; synaptic vesicle docking during exocytosis; synaptic transmission. 30 alleles are reported. The phenotypes of these alleles are annotated with: neuromuscular junction; t-bar. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References 80-80 h47   (Drosophila Heterochromatin Genome Project, 2004)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Snap25 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Snap25-RA FBtr0111143  NM_001043176   2184   212 Snap25-RB FBtr0111142  NM_001043177   2095   153
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Risinger et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Snap25-PA   FBpp0110435   23.7   212   4.24     NP_001036641   EAA46071 Snap25-PB   FBpp0110434   17.4   153   4.59     NP_001036642   EAL24571
Additional Polypeptide Data & Comments
Reported size (kD)	212 (aa); 23.7 (kD predicted) (Risinger et al., 1993) 25 (kD observed) (Risinger et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Target SNARE coiled-coil region (IPR000727) SNAP-25 (IPR000928)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567465   AABU01002755 EAA46071   AC004349   AI403350   AI514081   BI616949   BI623542   BT023789   CO176876   CO179250   CO188066   CO188894   CO190119   CO193159   CO263347   CO263569   CO264499   CO264576   CO264757   CO265615   CO268571   CO271848   CO279622   CO288287   CO290158   CO290776   CO291278   CO296257   CO297304   CO299380   CO300306   CO303413   CO303574   CO309745   CO312704   CO312732   CO314821   CO316664   CO319500   CO322410   CO322508   CO328629   CO331327   CO333684   CO342387   CO345201   CO345407   CO345450   DN153816   EC058857   EC059290   EC059291   EC062209   EC062444   EC064434   EC064533   EC064901   EC065043   EC069232   EC079400   EC079599   EC080828   EC081894   EC083869   EC083936   EC084288   EC087300   EC088375   EC088614   EC090998   EC091106   EC091108   EC195138   EC198437   EC205098   EC206817   EC213815   EC214461   EC215379   EC217115   EC221054   EC228989   EC230314   EC230480   EC230596   EC230878   EC230974   EC235000   EC235013   EC244142   EC244282   EC248524   EC250275   EC256415   L22021   U81146   U81147 AAB39757   U81148 AAB39757   U81149 AAB39757   U81150 AAB39757   U81151 AAB39757   U81152 AAB39757   U81153 AAB39757
UniProtKB/Swiss-Prot	P36975
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage  optic lobe (Risinger et al., 1993)  adult stage  perikaryon <of> abdominal neuromere (Risinger et al., 1993)  adult stage  suboesophageal ganglion (Risinger et al., 1993)  adult stage  perikaryon <of> thoracic ganglion (Risinger et al., 1993)  larval stage   (Risinger et al., 1993)  embryonic stage | stage>=14  ventral nerve cord (Risinger et al., 1997)  embryonic stage | stage>=14  neuron (Risinger et al., 1997)  adult stage  adult brain (Risinger et al., 1993)  adult stage   (Risinger et al., 1993)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage >=14  ventral nerve cord (Risinger et al., 1997)  embryonic stage | stage 16  chordotonal organ (Risinger et al., 1997)
Marker for
	synapse
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI517763 AI133982
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011288 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0011288 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0011288
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Snap25hs.PW lethal (with Df(3L)1-16) Snap25153 Snap2533 Snap253 Snap2553 Snap2593 lethal | recessive Snap251-16-0 Snap25153 Snap2516 Snap2522 Snap2527 Snap2533 Snap2534 Snap253 Snap2542 Snap2553 Snap25695 Snap2570 Snap2593 lethal | pharate adult stage (with Df(3L)1-16) Snap251-16-0 Snap25114 Snap25124 Snap25125 Snap25126 Snap25132 Snap25133 Snap25147 Snap2514 Snap25164 Snap2516 Snap2522 Snap2527 Snap2534 Snap2542 Snap2544 Snap2546 Snap2548 Snap2552 Snap25695 Snap2570 Snap2587 lethal | pharate adult stage | recessive Snap25114 Snap25124 Snap25125 Snap25126 Snap25132 Snap25133 Snap25147 Snap2514 Snap25164 Snap2544 Snap2546 Snap2548 Snap2552 Snap2587 Other Phenotypes Allele paralytic | recessive | conditional - heat sensitive Snap25ts paralytic | conditional - heat sensitive Snap25ts paralytic | conditional - heat sensitive (with Snap25ts) Snap251-16-0 Snap25114 Snap25124 Snap25125 Snap25126 Snap25132 Snap25133 Snap25147 Snap2514 Snap25153 Snap25164 Snap2516 Snap2522 Snap2527 Snap2533 Snap2534 Snap253 Snap2542 Snap2544 Snap2546 Snap2548 Snap2552 Snap2553 Snap25695 Snap2570 Snap2587 Snap2593 paralytic | conditional - heat sensitive (with Snap2593) Snap25ts paralytic | conditional - heat sensitive (with Snap2587) Snap25ts paralytic | conditional - heat sensitive (with Snap2570) Snap25ts paralytic | conditional - heat sensitive (with Snap25695) Snap25ts paralytic | conditional - heat sensitive (with Snap2553) Snap25ts paralytic | conditional - heat sensitive (with Snap2552) Snap25ts paralytic | conditional - heat sensitive (with Snap2548) Snap25ts paralytic | conditional - heat sensitive (with Snap2546) Snap25ts paralytic | conditional - heat sensitive (with Snap2544) Snap25ts paralytic | conditional - heat sensitive (with Snap2542) Snap25ts paralytic | conditional - heat sensitive (with Snap253) Snap25ts paralytic | conditional - heat sensitive (with Snap2534) Snap25ts paralytic | conditional - heat sensitive (with Snap2533) Snap25ts paralytic | conditional - heat sensitive (with Snap2527) Snap25ts paralytic | conditional - heat sensitive (with Snap2522) Snap25ts paralytic | conditional - heat sensitive (with Snap2516) Snap25ts paralytic | conditional - heat sensitive (with Snap25164) Snap25ts paralytic | conditional - heat sensitive (with Snap25153) Snap25ts paralytic | conditional - heat sensitive (with Snap2514) Snap25ts paralytic | conditional - heat sensitive (with Snap25147) Snap25ts paralytic | conditional - heat sensitive (with Snap25133) Snap25ts paralytic | conditional - heat sensitive (with Snap25132) Snap25ts paralytic | conditional - heat sensitive (with Snap25126) Snap25ts paralytic | conditional - heat sensitive (with Snap25125) Snap25ts paralytic | conditional - heat sensitive (with Snap25124) Snap25ts paralytic | conditional - heat sensitive (with Snap25114) Snap25ts paralytic | conditional - heat sensitive (with Snap251-16-0) Snap25ts neurophysiology defective Snap25124 Snap25ts neurophysiology defective | temperature conditional (with Df(3L)1-16) Snap25ts neuroanatomy defective Snap25ts Phenotype manifest in Allele neuromuscular junction Snap25124 Snap25ts neuromuscular junction | temperature conditional (with Df(3L)1-16) Snap25ts t-bar Snap25ts
Classical Alleles ( 28 )
For All Classical Alleles Show
Allele of Snap25 Class Mutagen Stocks Known lesion Snap251-16-0 X ray 0 -- Snap25114 X ray 0 -- Snap25124 X ray 0 -- Snap25125 X ray 0 -- Snap25126 X ray 0 -- Snap25132 X ray 0 -- Snap25133 X ray 0 -- Snap25147 X ray 0 -- Snap2514 X ray 0 -- Snap25153 X ray 0 -- Snap25164 X ray 0 -- Snap2516 X ray 0 -- Snap2522 X ray 0 -- Snap2527 X ray 0 -- Snap2533 X ray 0 -- Snap2534 X ray 0 -- Snap253 X ray 0 -- Snap2542 X ray 0 -- Snap2544 X ray 0 -- Snap2546 X ray 0 -- Snap2548 X ray 0 -- Snap2552 X ray 0 -- Snap2553 X ray 0 -- Snap25695 ethyl methanesulfonate 0 -- Snap2570 X ray 0 -- Snap2587 X ray 0 -- Snap2593 X ray 0 -- Snap25ts 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Snap25 Class Mutagen Stocks Known lesion Snap25hs.PW in vitro construct | regulatory fusion 0 Yes Snap25n-syb.PR in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)1-16 (Deitcher, 2001.10.21, Rao et al., 2001) Df(3L)Snap25153 (Vilinsky et al., 2002) Df(3L)Snap2533 (Vilinsky et al., 2002) Df(3L)Snap253 (Vilinsky et al., 2002) Df(3L)Snap2553 (Vilinsky et al., 2002)
Not disrupted in	Df(3L)8A-80 (Deitcher, 2001.10.21)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{n-syb-Snap25.R} NA heat-shock construct P{hs-Snap25.W} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Snap25 has been cloned and sequenced, and its RNA expression pattern has been analysed. (Risinger et al., 1993)