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Gene Dmel\pelo

General Information
Symbol	Dmel\pelo	Species	D. melanogaster
Name	pelota	Annotation symbol	CG3959
Feature type	protein_coding_gene	FlyBase ID	FBgn0011207
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	30C5-30C5	Sequence location	2L:9,700,796..9,708,861 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pelota is referred to in FlyBase by the symbol pelo (CG3959, FBgn0011207). It has the cytological map location 30C5. Its sequence location is 2L:9700796..9708861. Its molecular function is unknown. It is involved in the biological processes described with 15 unique terms, many of which group under: cell cycle; cell cycle process; M phase of meiotic cell cycle; cell division; gamete generation; anatomical structure development; sensory organ development; meiosis; regulation of developmental process; cellular macromolecule metabolic process. 15 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: organ system; anatomical structure; germarium; peripheral nervous system; nervous system; spermatocyte; cytoplasmic part; adult; follicle cell; intracellular membrane-bounded organelle. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 30C5-30C5   Limits computationally determined from genome sequence between P{EP}EP361&P{EP}peloEP2160 and P{lacW}zf30Ck02506&P{EP}undEP424
Experimentally Determined Cytological Location
Cytogenetic map Notes References 30C1-30C9 (determined by in situ hybridisation)   (Spradling et al., 1999) 30C1-30C9   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\pelo for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) pelo-RA FBtr0079847  NM_057634   2037   395
Additional Transcript Data & Comments
Reported size (kB)	2.1 (northern blot) (Eberhart and Wasserman, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein pelo-PA   FBpp0079444   44.2   395   5.34     NP_476982   AAF52799
Additional Polypeptide Data & Comments
Reported size (kD)	395 (aa); 49 (kD observed); 44 (kD predicted) (Eberhart and Wasserman, 1995)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0011207
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Probable translation factor pelota (IPR004405) eRF1 domain 1 (IPR005140) eRF1 domain 2 (IPR005141) eRF1 domain 3 (IPR005142)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA441609   AA696175   AA696181   AA801742   AA802378   AA979595   AC007291   AE003625 AAF52799   AI403075   AI546320   AI945735   AQ026402   AQ072946   AW943226   AY058644 AAL13873   BF505403   BH854617   BH854618   BI357196   BI370518   BI482749   BI483185   CK605331   CK605332   CK661684   CO182478   CO321319   CO335568   CZ479903   CZ479904   CZ481995   CZ481996   CZ490256   CZ490257   DU708619   EC244143   EC267716   U27197 AAC46879   UT01851
UniProtKB/Swiss-Prot	P48612
UniProtKB/TrEMBL
Maps to	AT07625 (Xi et al., 2005) FBcl0297508 (FlyBase, 1992-)
Does NOT map to
Identified with	bs16c04 (Oliver, 2001.8.16) LD34262 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References rescue fragment pelo[+t15] 2L:9,698,013..9,712,428 comment=Approximately 15kb genomic DNA fragment from lambdaG-3, probable SacI partial. evidence=experimental linked_to=SacI-SacI_rfrag (Eberhart and Wasserman, 1995)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7034
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Eberhart and Wasserman, 1995)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA979595
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0011207 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0011207 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0011207
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable pelo1 peloLA00223 semi-lethal pelo1 Other Phenotypes Allele wild-type pelo+t15 cell death increase pelo1 meiotic cell cycle defective pelo1 meiotic cell cycle defective | recessive pelo1 pelo2 pelo3 Sterility Allele female sterile pelo1 female sterile | conditional - cold sensitive pelo1 female sterile | partially pelo1 male sterile pelo1 male sterile | recessive pelo1 fertile peloLA00223 Phenotype manifest in Allele female germline stem cell (with Df(2L)s1402) pelo1 female germline stem cell pelo1 germline cyst pelo1 follicle cell | somatic clone pelo1 egg chamber pelo1 spermatocyte pelo1 pelo2 pelo3 spermatid pelo1 pelo2 pelo3 eye pelo1 ommatidium pelo1 interommatidial bristle pelo1 ovary pelo1 germarium pelo1 Nebenkern pelo1
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of pelo Class Mutagen Stocks Known lesion pelo1 loss of function P-element activity 3 Yes pelo2 loss of function Δ2-3 0 Yes pelo3 loss of function Δ2-3 0 Yes peloEP2160 P-element activity 1 -- peloKG06646 P-element activity 1 -- peloLA00223 P-element activity 1 -- peloe02080 piggyBac transposase 1 -- peloe04250 piggyBac transposase 1 -- pelof07516 piggyBac transposase 1 -- pelounspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of pelo Class Mutagen Stocks Known lesion pelo+t15 in vitro construct | genomic fragment 0 Yes peloGD11173 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes peloScer\UAS.P\T.T:Zzzz\FLAG,T:Hsap\MYC in vitro construct | coding region fusion 0 Yes peloScer\UAS.P\T.cXa in vitro construct | regulatory fusion 0 Yes pelonls.Scer\UAS.P\T.T:Zzzz\FLAG,T:Hsap\MYC in vitro construct | amino acid replacement 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)s1402
Disrupted in	Df(2L)30A-C (Spradling et al., 1999, Castrillon et al., 1993, BDGP Project Members, 1994-1999) Df(2L)Exel6024 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11173} NA P{UASp-pelo.FLAG,MYC} NA P{UASp-pelo.FM.nls} NA P{UASp-pelo.X} NA characterization construct P{pelo+G15} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}peloe02080 NA PBac{RB}peloe04250 NA PBac{WH}pelof07516 NA P{SUPor-P}peloKG06646 NA insertion of enhancer trap P{PZ}pelo1 Yes insertion of mobile activating element P{EP}peloEP2160 NA P{Mae-UAS.6.11}peloLA00223 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Meiotic entry defect. (Castrillon et al., 1993) pelo locus encodes a protein required for meiotic cell division. (Eberhart and Wasserman, 1995)
Gene Ontology: Function, Process & Cellular Component ( 17 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
compound eye development non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
compound eye morphogenesis inferred from mutant phenotype (Eberhart and Wasserman, 1995)
female germ-line stem cell division inferred from mutant phenotype (Xi et al., 2005)
germ-line stem cell maintenance inferred from mutant phenotype (Xi et al., 2005)
male meiosis inferred from mutant phenotype (Castrillon et al., 1993)
male meiosis I traceable author statement (Fuller, 1998)
meiosis inferred from mutant phenotype (Eberhart and Wasserman, 1995)
meiotic G2/MI transition traceable author statement (Fuller, 1998)
meiotic nuclear envelope disassembly inferred from mutant phenotype (Eberhart and Wasserman, 1995)
meiotic spindle organization and biogenesis inferred from mutant phenotype (Eberhart and Wasserman, 1995)
mitosis non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
spermatocyte division inferred from mutant phenotype (Eberhart and Wasserman, 1995)
spermatogenesis inferred from mutant phenotype (Castrillon et al., 1993) non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
spindle assembly involved in male meiosis I non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
translation inferred from sequence or structural similarity with SGD_LOCUS:DOM34; SGD:S0004946 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Xi et al., 2005)
nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) inferred from electronic annotation with InterPro:IPR004405 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions pelo allele Gene References unspecified dpp (Xi et al., 2005) bam (Xi et al., 2005)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 60384
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22793 Dere\GG10049 Dgri\GH25139 Dmoj\GI20011 Dper\GL18928 Dpse\GA17805 Dsec\GM17647 Dsim\GD23623 Dvir\GJ13438 Dwil\GK24186 Dyak\GE18863
Curated drosophilid orthologs	Dpse\GA17805
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000006742 Anopheles gambiae (Ensembl) ENSBTAP00000004233 Bos taurus (Ensembl) ENSCAFP00000027235 Canis familiaris (Ensembl) ENSCINP00000003991 Ciona intestinalis (Ensembl) NEWSINFRUP00000162152 Takifugu rubripes (Ensembl) ENSMMUP00000002343 Macaca mulatta (Ensembl) ENSMODP00000024257 Monodelphis domestica (Ensembl) ENSRNOP00000038406 Rattus norvegicus (Ensembl) GSTENP00035051001 Tetraodon nigroviridis (Ensembl) ENSXETP00000039788 Xenopus tropicalis (Ensembl) Q7XR03 Oryza sativa (Gramene) Q8S3N8 Oryza sativa (Gramene) AT4G27650.1 Arabidopsis thaliana (Arabidopsis) WBGene00039218 Caenorhabditis briggsae (Wormbase) WBGene00011280 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-2006 Danio rerio (Zfin) ZDB-GENE-040426-1074 Danio rerio (Zfin) DDB0205206 Dictyostelium discoideum (Dictybase) MGI:2145154 Mus musculus (Informatics) YNL001W Saccharomyces cerevisiae (Yeastgenome) SPCC18B5.06 Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Szeged	- P{EP}peloEP2160
Harvard	- PBac{RB}peloe02080 - PBac{RB}peloe04250 - PBac{WH}pelof07516
VDRC	v34770 w1118; P{GD11173}v34770 v34771 w1118; P{GD11173}v34771
Kyoto	108877 P{PZ}pelo1 cn1/CyO; ry506 101998 P{PZ}pelo1 cn1 / CyO ; ry506
Bloomington	11757 P{PZ}pelo1 cn1/CyO; ry506 14495 y1 w67c23; P{SUPor-P}peloKG06646 22224 y1 w*; P{Mae-UAS.6.11}peloLA00223
Genomic Clones ( 2 )
	BACR02I05 BACR24A22
cDNA Clones ( 19 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD34262
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD47870 RE57165 GM05232 LP21337 LD16595 GM05224 GM03432 RE65488 AT07625 RE66036 GM04288 RE43610 GH22440
Other clones	EK290807 UT01851 EC22761 bs16c04 EN10734
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0011207 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0011207
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
	Genetic analysis indicates that pelo controls female germline stem cell renewal by repressing a bam-independent pathway. (Xi et al., 2005)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) •