Gene Dmel\dia

Help

Close All Open All

General Information

Symbol

Dmel\dia

Species

D. melanogaster

Name

diaphanous

Annotation symbol

CG1768

Feature type

protein_coding_gene

FlyBase ID

FBgn0011202

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

38E7-38E8

Sequence location

2L:20,758,147..20,767,523 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene diaphanous is referred to in FlyBase by the symbol dia (CG1768, FBgn0011202). It has the cytological map location 38E7-38E8. Its sequence location is 2L:20758147..20767523. Its molecular function is described as: actin binding; Rho GTPase binding. It is involved in the biological processes described with 13 unique terms, many of which group under: cell cycle; cell division; organelle organization and biogenesis; actin filament-based process; cell cycle process; multicellular organismal development; gamete generation; sexual reproduction; pole cell development; protein localization; cytoskeleton organization and biogenesis; anatomical structure development. 20 alleles are reported. The phenotypes of these alleles are annotated with 22 unique terms, many of which group under: organ system; anatomical structure; blastoderm embryo; syncytial blastoderm embryo; cuticle; follicle cell; gastrula embryo; embryonic head; spermatocyte; cytoplasmic part; larval dorsal trunk; cleavage stage embryo; imaginal precursor; peripheral nervous system. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38E7-38E8

Limits computationally determined from genome sequence between P{PZ}Hr38⁰²³⁰⁶&P{PZ}dia¹ and P{EP}CG31673^EP432

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

38A1-39E1

Location based on deficiency mapping.

(Giansanti et al., 2004)

38E5-38E6

(determined by in situ hybridisation)

(Spradling et al., 1999)

38E1-38E10

38E5--6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

(Giansanti et al., 2004)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: CG9323- dia+ anon-38E.17+

(Butler et al., 2001)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: fod? dia+

(Castrillon and Wasserman, 1994)

Gene Model & Products

Please see the GBrowse view of Dmel\dia for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

dia-RA

FBtr0081410

NM_057633

4727

1091

dia-RB

FBtr0081411

NM_165341

4230

1091

Additional Transcript Data & Comments

Reported size (kB)

4.8, 4.4 (northern blot)

(Castrillon and Wasserman, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

dia-PA

FBpp0080940

123.2

1091

8.25

NP_476981

AAF53922

dia-PB

FBpp0080941

123.2

1091

8.25

NP_724285

AAN11087

Additional Polypeptide Data & Comments

Reported size (kD)

1091 (aa); 123 (kD predicted)

(Castrillon and Wasserman, 1994)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0011202

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Actin-binding FH2 and DRF autoregulatory (IPR003104)

DRF autoregulatory (IPR010465)

Diaphanous FH3 (IPR010472)

Diaphanous GTPase-binding (IPR010473)

Diaphanous autoregulatory (IPR014767)

GTPase-binding/formin homology 3 (IPR014768)

Actin-binding FH2 (IPR015425)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P48608

UniProtKB/TrEMBL

Q5BI26

Maps to

DS04217

(FlyBase, 1992-, Butler et al., 2001)

FBcl0297600

(FlyBase, 1992-)

Does NOT map to

Identified with

(Oliver, 2001.8.16)

LD14246

(BDGP Project Members, 2000-, Butler, 2001)

LD16963

(Spradling et al., 1999)

SD13607

(Somogyi and Rorth, 2004)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

dia[3]
2L:20,755,758..20,761,488

comment=The approximate boundaries of the dia[3] deletion map to a BamHI-XbaI restriction fragment; the position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.
evidence=experimental

(Castrillon and Wasserman, 1994)

deletion

dia[9]
2L:20,758,301..20,761,488

comment=The approximate boundaries of the dia[9] deletion map to a NheI-XbaI restriction fragment; the position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.
evidence=experimental
linked_to=NheI-XbaI_rfrag

(Castrillon and Wasserman, 1994)

deletion

dia[2]
2L:20,758,301..20,763,848

comment=The approximate boundaries of the dia[2] deletion map to a NheI-XbaI restriction fragment; the position of the restriction fragment on the reference sequence was inferred by a FlyBase curator.
evidence=experimental
linked_to=NheI-BamHI_rfrag

(Castrillon and Wasserman, 1994)

rescue fragment

dia[+t11]
2L:20,756,968..20,767,968

comment=The 5' boundary or the 11kb genomic dia rescue fragment is approximate. The rescue fragment is reported as an 11kb genomic fragment terminating in a SalI site; the position of the rescue fragment on the reference sequence was inferred by a FlyBase curator.
evidence=experimental
linked_to=START-SalI_rfrag

(Castrillon and Wasserman, 1994)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8245

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage | female

(Castrillon and Wasserman, 1994)

adult stage | male

(Castrillon and Wasserman, 1994)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0011202

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0011202

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0011202

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

dia^EY11231

lethal | maternal effect | recessive

dia⁹

lethal | recessive

lethal | embryonic stage | non-rescuable maternal effect | recessive

dia⁵

lethal | pupal stage | recessive

Other Phenotypes

Allele

wild-type

dia^+t11

meiotic cell cycle defective | male (with Df(2L)TW84)

dia^Z5930

meiotic cell cycle defective | male | recessive

mitotic cell cycle defective

dia^dsRNA.cHa

mitotic cell cycle defective | male (with Df(2L)TW84)

dia^Z5930

mitotic cell cycle defective | male | recessive

dia^Z4053

cytokinesis defective | recessive

cytokinesis defective | male (with Df(2L)TW84)

dia^Z5930

cytokinesis defective | male | recessive

Sterility

Allele

female sterile | recessive

dia¹

male sterile

dia¹

male sterile | recessive

dia¹

fertile

dia^EY11231

Phenotype manifest in

Allele

testis

dia¹

egg chamber

dia¹

spermatocyte

dia¹

spermatid

dia¹

Nebenkern

follicle cell

dia¹

contractile ring & meiotic cell cycle | male

cyst cell

dia¹

imaginal disc

larval central nervous system & neuroblast

spindle & meiotic cell cycle | male

furrow canal | germ-line clone

dia⁵

cycle 11 embryo | germ-line clone

dia⁵

embryo & cytoskeleton | germ-line clone

dia⁵

embryo & nucleus | germ-line clone

dia⁵

pole cell | germ-line clone

dia⁵

gastrula embryo | germ-line clone

dia⁵

dorsal closure embryo | germ-line clone

dia⁵

embryonic head | germ-line clone

dia⁵

denticle belt | germ-line clone

dia⁵

embryonic/first instar larval cuticle | germ-line clone

dia⁵

actin filament & embryo | germ-line clone

dia⁵

microtubule & embryo | germ-line clone

dia⁵

centrosome & embryo | germ-line clone

dia⁵

pole bud | germ-line clone

dia⁵

blastoderm embryo | germ-line clone

dia⁵

eye

dia⁹

border follicle cell, with Scer\GAL4^slbo.2.6

dia^{CA.Scer\UAS.T:Ivir\HA1}

larval dorsal trunk, with Scer\GAL4^btl.PS

dia^{CA.Scer\UAS.T:Ivir\HA1}

tracheal fusion cell, with Scer\GAL4^btl.PS

dia^{CA.Scer\UAS.T:Ivir\HA1}

spermatid & nucleus | supernumerary

spermatid & nucleus | supernumerary (with Df(2L)TW84)

dia^Z5930

Nebenkern (with Df(2L)TW84)

dia^Z5930

spindle & meiotic cell cycle | male (with Df(2L)TW84)

dia^Z5930

contractile ring & meiotic cell cycle | male (with Df(2L)TW84)

dia^Z5930

S2 cell-line & spindle microtubule

dia^dsRNA.cHa

Classical Alleles ( 16 )

For All Classical Alleles Show

Allele of dia	Class	Mutagen	Stocks	Known lesion
dia¹	hypomorph	P-element activity	2	Yes
dia²	amorph	Δ2-3	0	Yes
dia³⁸		ethyl methanesulfonate	0	--
dia³	hypomorph	Δ2-3	0	Yes
dia⁴⁰		ethyl methanesulfonate	0	--
dia⁵⁰		ethyl methanesulfonate	0	--
dia⁵⁵		ethyl methanesulfonate	0	--
dia⁵	loss of function, hypomorph	Δ2-3	1	Yes
dia⁹	hypomorph	Δ2-3	0	Yes
dia^EY11231		P-element activity	0	--
dia^GS2135			0	Yes
dia^Z4053		ethyl methanesulfonate	0	--
dia^Z5930		ethyl methanesulfonate	0	--
dia^k07135		P-element activity	3	--
dia^k07140		P-element activity	1	--
dia^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of dia	Mutagen	Stocks	Known lesion
dia^+t11	in vitro construct \| genomic fragment	0	Yes
dia^{CA.Scer\UAS.T:Ivir\HA1}	in vitro construct \| regulatory fusion	0	Yes
dia^GD9442	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
dia^dsRNA.cHa	in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(2L)DS6

Useful duplication

Dp(2;f)Bl

Disrupted in

Df(2L)DS5

(Butler et al., 2001)

Df(2L)DS6

(Kozlova et al., 1998)

Df(2L)DS9

(Kozlova et al., 1998, Butler et al., 2001)

Df(2L)Fs(2)Ket-RX32

(Kozlova et al., 1998, Butler et al., 2001)

Df(2L)TW161

(BDGP Project Members, 1994-1999)

Df(2L)TW2

(Butler et al., 2001)

Df(2L)TW65

(Butler et al., 2001)

Df(2L)TW84

(Butler et al., 2001, Giansanti et al., 2004, Castrillon et al., 1993, BDGP Project Members, 1994-1999, Castrillon and Wasserman, 1994)

Df(2L)VA6

(Butler et al., 2001, BDGP Project Members, 1994-1999)

Df(2L)pr11163

(Butler et al., 2001)

Df(2L)pr1122

(Butler et al., 2001)

Df(2L)pr9204

(Butler et al., 2001)

Not disrupted in

Df(2L)Acon-21

(Butler et al., 2001)

Df(2L)DS8

(Butler et al., 2001)

Df(2L)TW50

(Butler et al., 2001)

Df(2L)pr-A16

(Butler et al., 2001)

Df(2L)pr1

(Butler et al., 2001)

Df(2L)pr2b

(Butler et al., 2001)

Df(2L)pr37

(Butler et al., 2001)

Df(2L)pr9201

(Butler et al., 2001)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9442}

P{UAS-dia.CA}

characterization construct

P{DC4}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{?PZ}dia⁵

insertion of enhancer trap

P{PZ}dia¹

Yes

P{lacW}dia^k07135

P{lacW}dia^k07140

P{lacW}l(2)SH0694^SH0694

insertion of mobile activating element

P{EPgy2}dia^EY11231

P{GSV1}dia^GS2135

Related Comments

Please look at the allele reports for the complete phenotype data

Proliferation defect locus.

(Castrillon et al., 1993)

dia function is required for cytokinesis.

(Castrillon and Wasserman, 1994)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: binucleate cells with increased or polarized (uneven) accumulation of F-actin.

(Kiger et al., 2003)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc₁₆₇ cells.

(Eggert et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 16 )

Molecular Function

CV term

Evidence

References

Rho GTPase binding

inferred from electronic annotation with InterPro:IPR010473

(FlyBase Curators et al., 2004-)

actin binding

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)