Gene Dmel\lwr

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General Information

Symbol

Dmel\lwr

Species

D. melanogaster

Name

lesswright

Annotation symbol

CG3018

Feature type

protein_coding_gene

FlyBase ID

FBgn0010602

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

21E2-21E2

Sequence location

2L:541,235..542,572 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene lesswright is referred to in FlyBase by the symbol lwr (CG3018, FBgn0010602). It has the cytological map location 21E2. Its sequence location is 2L:541235..542572. Its molecular function is described as: SUMO conjugating enzyme activity; protein binding; small conjugating protein ligase activity. It is involved in the biological processes described with 12 unique terms, many of which group under: anatomical structure development; biopolymer modification; cell cycle; M phase of meiotic cell cycle; protein modification process; regulation of biological process; female meiosis; cellular macromolecule metabolic process; immune response; zygotic determination of dorsal/ventral axis; negative regulation of Toll signaling pathway; negative regulation of hemocyte proliferation; nucleocytoplasmic transport; regulation of metabolic process. 24 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: anatomical structure; organ system; embryonic/larval hemocoel; hemocyte; thoracic segment; adult; adult segment; embryonic tagma; embryonic segment; embryonic head; embryonic abdomen; peripheral nervous system. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21E2-21E2

Limits computationally determined from genome sequence between P{lacW}l(2)k09610^k09610&P{lacW}S^k09538a and P{PZ}l(2)10685¹⁰⁶⁸⁵&P{lacW}Tango14^k00619

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

21C-21C

(Bhaskar, 1999.12.27)

21C-21D

(Ohsako, 1998.9.11)

21C-21D

(Ohsako, 1998.9.11)

21D-21D

(Joanisse, 1997.10.20)

21C6-21C7

(determined by in situ hybridisation)

(Spradling et al., 1999)

21C-21D

(determined by in situ hybridisation)

(Ohsako and Takamatsu, 1999)

21D-21D

(determined by in situ hybridisation)

(Joanisse et al., 1998)

21C-21C

(Apionishev and Rasooly, 1996)

21C6-21C7

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

(Apionishev and Rasooly, 1997, Epps and Tanda, 1998)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: lwr- Spp-

(Apionishev et al., 2001)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: lwr+ ush-

(Epps and Tanda, 1998)

Overall orientation not stated: lwr- Spp-

(Sun et al., 2003)

Gene Model & Products

Please see the GBrowse view of Dmel\lwr for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

lwr-RA

FBtr0078081

NM_057630

1259

159

lwr-RB

FBtr0078082

NM_164385

1288

159

Additional Transcript Data & Comments

Reported size (kB)

0.736 (longest cDNA)

(Joanisse et al., 1998)

1.2 (northern blot)

(Epps and Tanda, 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

lwr-PA

FBpp0077741

18.1

159

8.61

NP_476978

AAN10499

lwr-PB

FBpp0077742

18.1

159

8.61

NP_722637

AAF51487

Additional Polypeptide Data & Comments

Reported size (kD)

159 (aa)

(Epps and Tanda, 1998, Bhaskar et al., 2000)

159 (aa); 18 (kD predicted)

(Joanisse et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010602

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-conjugating enzyme, E2 (IPR000608)

Ubiquitin-conjugating enzyme/RWD-like (IPR016135)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7KNM2

Maps to

(FlyBase, 1992-)

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

GM08125

(Spradling et al., 1999)

LD06144

(Apionishev et al., 2001)

LD12093

(Epps and Tanda, 1998, Apionishev et al., 2001)

Dm0628

(BDGP Project Members, 1994-1999, Spradling et al., 1999)

FGM209H11

(Mukai et al., 2006)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

aberration junction

Df(2L)lwr[14].bk1
2L:542,248..542,560

comment=Position of mutation on reference sequence inferred by FlyBase curator based on author statement: 1136bp deletion starting from the site of the P{PZ}lwr[05486] insertion and extending proximally, removing part of the first exon of lwr and extending into shanti.
evidence=experimental

(Apionishev et al., 2001)

point mutation

lwr[5]
2L:541,937..541,937

evidence=experimental
na_change=G541937A
pr_change=R104H|lwr-PA,R104H|lwr-PB
reported_na_change=G?A
reported_pr_change=R104H

(Apionishev et al., 2001)

rescue fragment

lwr[+t2.4]
2L:540,761..543,142

comment=2.4kb genomic fragment containing the entire lwr coding region. This genomic fragment also contains a 3' portion of the Spp gene.
evidence=experimental

(Casso et al., 2005)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

5818

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage

(Bhaskar et al., 2000)

embryonic stage | syncytial blastoderm

(Epps and Tanda, 1998)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage

(Joanisse et al., 1998)

adult stage | F

(Joanisse et al., 1998)

Marker for

Subcellular Localization

CV Term

nucleus

(Joanisse et al., 1998)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI062879

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010602

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010602

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010602

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | maternal effect | recessive

lwr⁰²⁸⁵⁸

lethal | recessive

lethal | recessive | rescuable maternal effect

lwr⁰²⁸⁵⁸

lethal | recessive | third instar larval stage

lwr¹³

lethal | embryonic stage, with Scer\GAL4^e33C

lwr^DN.Scer\UAS

lethal | embryonic stage, with Scer\GAL4^tub

lwr^DN.Scer\UAS

lethal | second instar larval stage (with lwr¹⁴)

lwr¹³

lethal | second instar larval stage (with lwr¹³)

lwr¹⁴

lethal | third instar larval stage

lethal | third instar larval stage (with lwr⁵)

lwr¹³

lethal | third instar larval stage (with lwr^4-3)

lwr¹³

lethal | third instar larval stage (with lwr¹³)

semi-lethal | recessive

lwr^unspecified

Other Phenotypes

Allele

wild-type

lwr^+LD12093

visible | recessive

lwr^unspecified

neuroanatomy defective | somatic clone

lwr⁰⁵⁴⁸⁶

melanotic 'tumor' | larval stage (with lwr⁵)

melanotic 'tumor' | larval stage (with lwr^4-3)

lwr⁵

melanotic 'tumor' | larval stage (with lwr¹⁴)

lwr⁵

melanotic 'tumor' | larval stage | partially (with lwr^4-3)

lwr¹⁴

melanotic 'tumor' | larval stage | partially (with lwr¹⁴)

lwr^4-3

developmental rate defective (with lwr⁵)

lwr^4-3

developmental rate defective (with lwr^4-3)

lwr⁵

Phenotype manifest in

Allele

embryonic epidermis

embryonic head

embryonic thoracic segment

embryonic abdominal segment

leg (with lwr^unspecified)

lwr⁰⁵⁴⁸⁶

adult head (with lwr^unspecified)

lwr⁰⁵⁴⁸⁶

wing (with lwr^unspecified)

lwr⁰⁵⁴⁸⁶

eye (with lwr^unspecified)

lwr⁰⁵⁴⁸⁶

neuroblast | somatic clone

lwr⁰⁵⁴⁸⁶

hemocyte | supernumerary (with lwr⁵)

lamellocyte | supernumerary (with lwr⁵)

crystal cell (with lwr⁵)

lamellocyte | supernumerary (with lwr^4-3)

hemocyte & nucleus | supernumerary (with lwr^4-3)

lwr¹⁴

crystal cell (with lwr^4-3)

larval hemocyte | supernumerary (with lwr⁵)

lwr^4-3

plasmatocyte | supernumerary (with lwr⁵)

lwr^4-3

melanotic mass & larva

lamellocyte | supernumerary (with lwr¹⁴)

hemocyte & nucleus | supernumerary (with lwr¹⁴)

lwr^4-3

crystal cell (with lwr¹⁴)

larval hemocyte | supernumerary (with lwr^4-3)

lwr⁵

plasmatocyte | supernumerary (with lwr^4-3)

lwr⁵

hemocyte | supernumerary (with lwr^4-3)

lwr⁵

hemocyte | supernumerary (with lwr¹⁴)

lwr⁵

embryonic epidermis, with Scer\GAL4^tub

lwr^DN.Scer\UAS

embryonic epidermis, with Scer\GAL4^e33C

lwr^DN.Scer\UAS

larval hemocyte | supernumerary, with Scer\GAL4^e33C

lwr^DN.Scer\UAS

larval hemocyte | supernumerary, with Scer\GAL4^Cg.PA

lwr^DN.Scer\UAS

leg (with lwr⁰⁵⁴⁸⁶)

lwr^unspecified

adult head (with lwr⁰⁵⁴⁸⁶)

lwr^unspecified

wing (with lwr⁰⁵⁴⁸⁶)

lwr^unspecified

eye (with lwr⁰⁵⁴⁸⁶)

lwr^unspecified

Classical Alleles ( 15 )

For All Classical Alleles Show

Allele of lwr	Class	Mutagen	Stocks	Known lesion
lwr^01519a		P-element activity	0	--
lwr⁰²⁸⁵⁸		P-element activity	1	Yes
lwr⁰⁵⁴⁸⁶	loss of function, hypomorph	P-element activity	1	Yes
lwr¹⁰⁷		P-element activity	0	Yes
lwr¹⁰	loss of function	Δ2-3	0	Yes
lwr¹¹⁸		P-element activity	0	Yes
lwr¹³	loss of function	Δ2-3	1	Yes
lwr¹⁴	loss of function	Δ2-3	0	Yes
lwr^4-3	hypomorph	Δ2-3	1	Yes
lwr⁵	hypomorph	ethyl methanesulfonate	1	--
lwr^DG29707		P-element activity	1	--
lwr^GS-A2612		Δ2-3	0	--
lwr^e02021		piggyBac transposase	1	--
lwr^semi710		P-element activity	0	Yes
lwr^unspecified		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of lwr	Mutagen	Stocks	Known lesion
lwr^+LD12093	in vitro construct \| other	0	Yes
lwr^+t2.4	in vitro construct \| genomic fragment	0	Yes
lwr^Act5C.PB	in vitro construct \| regulatory fusion	0	Yes
lwr^{Act5C.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
lwr^DN.Scer\UAS	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	0	Yes
lwr^GD10017	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
lwr^{MtnA.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0