Gene Dmel\TpnC73F

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General Information

Symbol

Dmel\TpnC73F

Species

D. melanogaster

Name

Troponin C at 73F

Annotation symbol

CG7930

Feature type

protein_coding_gene

FlyBase ID

FBgn0010424

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

73E5-73E5

Sequence location

3L:17,039,264..17,041,490 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Troponin C at 73F is referred to in FlyBase by the symbol TpnC73F (CG7930, FBgn0010424). It has the cytological map location 73E5. Its sequence location is 3L:17039264..17041490. Its molecular function is described as calcium ion binding. It is involved in the biological process ciliary or flagellar motility. 4 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

73E5-73E5

Limits computationally determined from genome sequence between P{PZ}Baldspot⁰²²⁸¹ and P{PZ}blot⁰¹⁶⁵⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

73F-73F

(Fyrberg, 1993.11.8)

73F-73F

(determined by in situ hybridisation)

(Fyrberg et al., 1994)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\TpnC73F for information on other features

Comments on Gene Model

DGC clone appears problematic (RE14813): unspliced intron

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

TpnC73F-RA

FBtr0089585

NM_079398

945

155

TpnC73F-RB

FBtr0089586

NM_168715

2091

Additional Transcript Data & Comments

Reported size (kB)

0.9 (northern blot)

(Fyrberg et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

TpnC73F-PA

FBpp0088546

17.7

155

3.81

NP_524122

AAF49371

TpnC73F-PB

FBpp0088547

7.3

3.98

NP_730242

AAN11732

Additional Polypeptide Data & Comments

Reported size (kD)

155 (aa)

(Fyrberg et al., 1994)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010424

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Calcium-binding EF-hand (IPR002048)

Flagellar calcium-binding protein (calflagin) (IPR003299)

EF-Hand type (IPR011992)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P47949

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE14813

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3791

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage

(Fyrberg et al., 1994)

pupal stage

(Fyrberg et al., 1994)

embryonic stage | late

(Fyrberg et al., 1994)

embryonic stage-adult stage

whole organism

(Qiu et al., 2003)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI258308

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010424

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010424

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010424

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for TpnC73F

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for TpnC73F

Stage(s) 9-10

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of TpnC73F	Class	Mutagen	Stocks	Known lesion
TpnC73F^MB03946			1	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of TpnC73F	Mutagen	Stocks	Known lesion
TpnC73F^GD10670	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
TpnC73F^GD12497	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
TpnC73F^GD16304	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)st7

Useful duplication

Dp(3;3)st⁺g18

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10670}

P{GD12497}

P{GD16304}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap binary system

Mi{ET1}TpnC73F^MB03946

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

calcium ion binding

non-traceable author statement

(Fyrberg, 1993.11.8)

non-traceable author statement

(Swiss-Prot Project Members, 1996.2.1)

inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR003299, InterPro:IPR011992

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

ciliary or flagellar motility

inferred from electronic annotation with InterPro:IPR003299

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

flagellin-based flagellum

inferred from electronic annotation with InterPro:IPR003299

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

TpnC73F allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65208

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10103

Dere\GG13604

Dgri\GH16402

Dmoj\GI12482

Dper\GL11919

Dpse\GA23964

Dsec\GM25686

Dsim\GD14691

Dvir\TpnC73F

Dwil\GK15267

Dyak\GE19899

Curated drosophilid orthologs

Dsub\TpnC73F

Dvir\TpnC73F

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000018434
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v23076

w¹¹¹⁸; P{GD12497}v23076

v34272

w¹¹¹⁸; P{GD10670}v34272

Bloomington

24549

w¹¹¹⁸; Mi{ET1}TpnC73F^MB03946/TM6C, Sb¹

Genomic Clones ( 2 )

cDNA Clones ( 10 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010424

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010424

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

Isolation and characterisation of Troponin C like gene TpnC73F.

(Fyrberg et al., 1994)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

P47949

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Calcium-binding EF-hand (IPR002048)

Flagellar calcium-binding protein (calflagin) (IPR003299)

EF-Hand type (IPR011992)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for TpnC73F

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65208

DEDB - Drosophila exon database: splicing graphs

•

3791

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010424

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010424

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010424

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0010424

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0010424

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010424

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010424

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000018434
Anopheles gambiae (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010424

Synonyms & Secondary IDs ( 12 )

Reported As

Symbol Synonym

CG7930

(Goldstein and Gunawardena, 2000, )

DmTnC3

(Qiu et al., 2003)

DmTpnC73F

(Herranz et al., 2004)

DmTpnCIa

(Herranz et al., 2004)

TnC

(Nongthomba et al., 2004, Ruiz et al., 2002)

TNC

(Diaz-Castillo and Golic, 2004)

TnC73F

(Fyrberg, 1993.11.8, )

TpnC73F

(Montana and Littleton, 2006, Dietzl et al., 2007)

TpnC Ia

(Herranz et al., 2004)

unnamed

(Vigoreaux, 2001)

Name Synonym

troponin C

(OOta and Saitou, 1999)

Troponin C at 73F

Secondary FlyBase IDs

References ( 29 )

Generate a list of

All references relating to this gene ( 29 )

List References by type

Recent research papers ( 2 )

Dietzl et al., 2007, Nature 448(7150): 151--156: A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Montana and Littleton, 2006, J. Biol. Chem. 281(12): 8100--8109: Expression profiling of a hypercontraction-induced myopathy in Drosophila suggests a compensatory cytoskeletal remodeling response. [FBrf0190896]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2006

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