Gene Dmel\Dro

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General Information

Symbol

Dmel\Dro

Species

D. melanogaster

Name

Drosocin

Annotation symbol

CG10816

Feature type

protein_coding_gene

FlyBase ID

FBgn0010388

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

51C1-51C1

Sequence location

2R:10,633,461..10,634,219 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Drosocin is referred to in FlyBase by the symbol Dro (CG10816, FBgn0010388). It has the cytological map location 51C1. Its sequence location is 2R:10633461..10634219. Its molecular function is unknown. It is involved in the biological processes: defense response to Gram-negative bacterium; defense response; defense response to Gram-positive bacterium; defense response to bacterium; antibacterial humoral response. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

51C1-51C1

Limits computationally determined from genome sequence between P{lacW}ttv^k03617&P{EP}ttv^EP765 and P{lacW}Rpn6^k00103

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

51C1-51C6

(Meister, 1996.6.11)

51C1-51C6

(determined by in situ hybridisation)

(Charlet et al., 1996)

Experimentally Determined Recombination Data

Location

(Meister, 1996.6.11)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Dro+ AttA+ AttB+

(Hedengren et al., 2000)

Gene Model & Products

Please see the GBrowse view of Dmel\Dro for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Dro-RA

FBtr0087436

NM_079020

759

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Dro-PA

FBpp0086566

7.1

11.81

NP_523744

AAF58216

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P36193

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RH31634

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12464

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010388

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010388

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010388

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^da.G32

Dro^Scer\UAS.cTa

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Dro	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dro	Mutagen	Stocks	Known lesion
Dro^GD15497	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
Dro^GD498	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Dro^Scer\UAS.cTa	in vitro construct \| regulatory fusion	0	Yes
Dro^mCa	in vitro construct \| minigene	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)L4

Useful duplication

Dp(2;2)SMG45

Disrupted in

Df(2R)Exel6284

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

UAS construct

characterization construct

P{Drc2.5-ORF-2}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Dro encodes a 19-residue proline-rich inducible antibacterial peptide, which has an O-glycosylation that is necessary for its full biological activity.

(Bulet et al., 1993)

Glycopeptides of Dro are isolated and the range of activities of these molecules is studied.

(Bulet et al., 1996)

Cloning and characterisation of Dro. 2.5kb of upstream sequence confers inducibility and tissue specificity to a transgene.

(Charlet et al., 1996)

Genes encoding antibacterial peptides are regulated in a manner distinct from that of Drs, encoding the antifungal peptide.

(Lemaitre et al., 1996)

Transcription is not strongly induced by hymenopteran parasitoids.

(Nicolas et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

antibacterial humoral response

inferred from expression pattern

(Lemaitre et al., 1997)

defense response

inferred from direct assay

(Uttenweiler-Joseph et al., 1998)

defense response to Gram-negative bacterium

inferred from direct assay

(Bulet et al., 1993)

inferred from expression pattern

(Lemaitre et al., 1997)

inferred from direct assay

(Levashina et al., 1995)

traceable author statement

(Meister et al., 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1994.6.1)

defense response to Gram-positive bacterium

inferred from direct assay

(Levashina et al., 1995)

defense response to bacterium

non-traceable author statement

(Kimbrell and Beutler, 2001)

traceable author statement

(Chapman, 2001)

Cellular Component

CV term

Evidence

References

extracellular region

inferred from direct assay

(Uttenweiler-Joseph et al., 1998)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Dro allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62381

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11338

Dere\GG20474

Dper\GL11457

Dpse\GA10577

Dsec\GM21566

Dsim\Dro

Dwil\GK19342

Dyak\GE13605

Curated drosophilid orthologs

Dpse\GA10577

Dsim\Dro

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v42503

w¹¹¹⁸; P{GD498}v42503

Genomic Clones ( 2 )

cDNA Clones ( 6 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RH31634

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RH29679

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010388

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010388

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Dro IM15

(Rabel et al., 2004)

Source for merge of: Dro BcDNA:RH31634 Source for merge of Dro BcDNA:RH31634 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Shows alteration in expression in a Clk mutant background.

(McDonald and Rosbash, 2001)

Other Comments

Ecol\lacZ^Dpt.PR adults are pricked with a sterile needle dipped in culture pellets of various living microorganisms (distinct bacterial strains, fungal spores or hyphae). Pricking results in a low but clearly detectable expression of all antimicrobial genes and these genes are induced above the background level by specific classes of microorganism.

(Lemaitre et al., 1997)

Identification: Encodes a protein that is induced by immune challenge.

(Uttenweiler-Joseph et al., 1998)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079020

RefSeq (Proteins)

•

NP_523744

DDBJ / EMBL / GenBank

UniProtKB/Swiss-Prot

•

P36193

Other Crossreferences

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62381

DEDB - Drosophila exon database: splicing graphs

•

12464

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010388

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010388

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010388

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0010388

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010388

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010388

University of Chicago Dev. Expression

•

FBgn0010388

Synonyms & Secondary IDs ( 19 )

Reported As

Symbol Synonym

143609_at

(Kristensen et al., 2005)

BcDNA:RH31634

CG10816

(Irving et al., 2001, De Gregorio et al., 2001, )

DIM 9

(Uttenweiler-Joseph et al., 1998)

DIM 11

(Uttenweiler-Joseph et al., 1998)

DIM-15

(Rabel et al., 2004)

DIM 15

(Uttenweiler-Joseph et al., 1998)

Drc

(Apidianakis et al., 2005, Tzou et al., 2002, Boulanger et al., 2001, Lemaitre et al., 1997, Lemaitre et al., 1996, Charlet et al., 1996)

drc

(Georgel et al., 2001)

dro

(McDonald and Rosbash, 2001, Khush and Lemaitre, 2000)

Dro

(Ramos et al., 2006)

DROC

(Tzou et al., 2000)

Dros

(Ramet et al., 2002)

drosocin

(Leclerc and Reichhart, 2004, Lemaitre, 2000.12.20, Meister, 1996.6.11, Hetru, 1993.3.9, Uttenweiler-Joseph et al., 1998, Bulet et al., 1993)

IM15

Name Synonym

Drosocin

(Tzou et al., 2002, Charlet et al., 1996, Pal et al., 2007, Waterhouse et al., 2007, Rutschmann et al., 2000)

Drosophila Immune induced molecule 9

(Uttenweiler-Joseph et al., 1998)

Drosophila Immune induced molecule 11

(Uttenweiler-Joseph et al., 1998)

Drosophila Immune induced molecule 15

(Uttenweiler-Joseph et al., 1998)

Secondary FlyBase IDs

FBgn0062947
FBgn0067904

References ( 72 )

Generate a list of

All references relating to this gene ( 72 )

List References by type

Recent research papers ( 3 )

Pal et al., 2007, J. Biol. Chem. 282(12): 8969--8977: Fungal peptide destruxin a plays a specific role in suppressing the innate immune response in Drosophila melanogaster. [FBrf0200357]
Waterhouse et al., 2007, Science 316(5832): 1738--1743: Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes. [FBrf0200242]
Ramos et al., 2006, Genetics 172(4): 2337--2349: Genomic organization of gypsy chromatin insulators in Drosophila melanogaster. [FBrf0190762]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2006

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