Gene Dmel\Cdc42

General Information
Symbol	Dmel\Cdc42	Species	D. melanogaster
Name	Cdc42	Annotation symbol	CG12530
Feature type	protein_coding_gene	FlyBase ID	FBgn0010341
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	18E1-18E1	Sequence location	X:19,591,117..19,593,285 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Cdc42 (CG12530, FBgn0010341). It has the cytological map location 18E1. Its sequence location is X:19591117..19593285. Its molecular function is described as: GTPase activity; GTP binding. It is involved in the biological processes described with 26 unique terms, many of which group under: anatomical structure development; actin filament-based process; organelle organization and biogenesis; cell projection organization and biogenesis; gamete generation; ovarian follicle cell development; cell motility; embryonic development via the syncytial blastoderm; cytoskeleton organization and biogenesis; sexual reproduction; cell communication. 28 alleles are reported. The phenotypes of these alleles are annotated with 53 unique terms, many of which group under: organ system; nervous system; adult segment; peripheral nervous system; embryonic nervous system; adult mesothoracic segment; embryonic neuron; cell projection; thoracic segment; antennal segment; late extended germ band embryo. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 18E1-18E1 Limits computationally determined from genome sequence between P{EP}Sec61γ^EP1511 and P{EP}EP1116&P{EP}EP1344^EP1344
Experimentally Determined Cytological Location
Cytogenetic map Notes References 18E-18E (determined by in situ hybridisation) (Sasamura et al., 1997) 18D-18D (determined by in situ hybridisation) (Sasamura, 1995)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Cdc42 for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cdc42-RA FBtr0074751 NM_078690 1399 191 Cdc42-RB FBtr0074752 NM_167677 1467 191
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Luo et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Cdc42-PA FBpp0074520 21.4 191 7.21 NP_523414 AAF49007 Cdc42-PB FBpp0074521 21.4 191 7.21 NP_728290 AAN09512
Additional Polypeptide Data & Comments
Reported size (kD)	191 (aa) (Luo et al., 1994)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0010341
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras GTPase (IPR001806) Ras small GTPase, Rho type (IPR003578) Small GTP-binding protein (IPR005225) Ras (IPR013753)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202963 AA440948 AA541063 AA697235 AA735197 AA735559 AA736181 AA802835 AA817478 AA820222 AA820843 AA950056 AA951293 AA951816 AA979233 AE003512 AAF49007 AE003512 AAN09512 AF153423 AAD43787 AF153424 AAD43788 AF153425 AAD43789 AF153426 AAD43790 AF153427 AAD43791 AF153428 AAD43792 AF153429 AAD43793 AI294616 AI455962 AI518944 AI543986 AY119570 AAM50224 BG636895 BG638741 BI142041 BI165021 BI167537 BI167879 BI171103 BI213171 BI214399 BI227843 BI240309 BI243832 BI362004 BI362469 BI364475 BI364667 BI373911 BI482013 BI609277 BI611157 BI632753 BI636385 BI637809 BI638392 BI639743 BI640331 BP540553 BQ103428 CO152867 CO154438 CO156812 CO185656 CO187235 CO189306 CO262445 CO267298 CO281747 CO283020 CO286587 CO294736 CO297701 CO302370 CO307725 CO311103 CO313009 CO322792 CO325286 CO329468 CO330096 CO334148 CO336621 CO336784 CO336826 CO337674 CO341024 CS026541 CAI61737 EC053406 EC057098 EC059591 EC061098 EC061473 EC064129 EC064171 EC073787 EC074060 EC076529 EC085523 EC088296 EC088400 EC090234 EC090977 EC199810 EC201635 EC219337 EC219338 EC226497 EC227874 EC236123 EC240905 EC245653 EC245654 EC247496 EC249041 EC253435 EC258234 EC258840 EC261258 EC261483 EC261577 EC261744 EC262821 EC266037 EC267822 ES688777 U11824 AAA62871

UniProtKB/Swiss-Prot	P40793
UniProtKB/TrEMBL

Maps to
Does NOT map to
Identified with	HL08128 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation Cdc42[1] X:19,592,019..19,592,019 evidence=experimental pr_change=G10D\|Cdc42-PA,G10D\|Cdc42-PB reported_na_change=G50A reported_pr_change=G10D na_change=G19592019A (Fehon et al., 1997, Genova et al., 2000) point mutation Cdc42[5] X:19,592,183..19,592,183 evidence=experimental pr_change=D65N\|Cdc42-PA,D65N\|Cdc42-PB reported_na_change=G214A reported_pr_change=D65N na_change=G19592183A (Genova et al., 2000) point mutation Cdc42[6] X:19,592,238..19,592,238 evidence=experimental pr_change=S83L\|Cdc42-PA,S83L\|Cdc42-PB reported_na_change=G269A reported_pr_change=S83L na_change=C19592238T (Genova et al., 2000) point mutation Cdc42[2] X:19,592,280..19,592,280 evidence=experimental reported_na_change=T311A na_change=T19592280A (Fehon et al., 1997, Genova et al., 2000) point mutation Cdc42[4] X:19,592,339..19,592,339 evidence=experimental reported_na_change=G370A na_change=G19592339A (Genova et al., 2000) point mutation Cdc42[3] X:19,592,392..19,592,392 evidence=experimental pr_change=G114D\|Cdc42-PA,G114D\|Cdc42-PB reported_na_change=G423A reported_pr_change=G114D na_change=G19592392A (Genova et al., 2000)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 3372
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage \| stage 10-12 somatic mesoderm (Luo et al., 1994) embryonic stage embryonic central nervous system (Luo et al., 1994) embryonic stage embryonic/larval digestive system (Luo et al., 1994) embryonic stage \| blastoderm (Luo et al., 1994) embryonic stage (Sasamura et al., 1997) embryonic stage-adult stage (Sasamura et al., 1997)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for

Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010341 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0010341 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0010341 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for Cdc42 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Cdc42 Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Cdc42² viable, with Scer\GAL4^hs.PB Cdc42^N17.Scer\UAS viable, with Scer\GAL4⁴⁵⁸ Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS viable, with Scer\GAL4³⁰⁶ Cdc42^N17.Scer\UAS viable, with Scer\GAL4²¹² Cdc42^N17.Scer\UAS lethal \| recessive Cdc42¹ Cdc42³ Cdc42⁴ lethal \| female, with Scer\GAL4³⁰⁶ Cdc42^V12.Scer\UAS lethal \| somatic clone Cdc42³ lethal \| partially, with Scer\GAL4^elav.PLu Cdc42^N17.Scer\UAS lethal \| embryonic stage \| maternal effect \| partially (with Cdc42⁶) Cdc42³ Cdc42⁴ lethal \| embryonic stage \| maternal effect \| partially (with Cdc42⁴) Cdc42⁶ lethal \| embryonic stage \| maternal effect \| partially (with Cdc42³) Cdc42⁶ lethal \| embryonic stage, with Scer\GAL4¹⁴⁰⁷, Scer\GAL4^how-24B Cdc42^V12.Scer\UAS lethal \| embryonic stage, with Scer\GAL4^69B Cdc42^N17.Scer\UAS lethal \| larval stage, with Scer\GAL4^sca-109-68 Cdc42^Scer\UAS.cLa lethal \| larval stage, with Scer\GAL4^109-79 Cdc42^Scer\UAS.cLa lethal \| larval stage, with Scer\GAL4^109-69 Cdc42^Scer\UAS.cLa lethal \| larval stage, with Scer\GAL4^109-53 Cdc42^Scer\UAS.cLa lethal \| larval stage, with Scer\GAL4^109-39 Cdc42^Scer\UAS.cLa lethal \| larval stage, with Scer\GAL4^109-30 Cdc42^Scer\UAS.cLa lethal \| pupal stage, with Scer\GAL4^how-24B Cdc42^N17.Scer\UAS lethal, with Scer\GAL4^hs.PB Cdc42^V12.Scer\UAS lethal, with Scer\GAL4^elav.PLu Cdc42^V12.Scer\UAS lethal, with Scer\GAL4^238Y Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS lethal, with Scer\GAL4^198Y Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS semi-lethal, with Scer\GAL4²¹² Cdc42^V12.Scer\UAS Other Phenotypes Allele wild-type Cdc42^Ubi-p63E.PF Cdc42^{Ubi-p63E.T:Hsap\MYC} visible Cdc42² Cdc42⁵ Cdc42⁶ Cdc42^GMR.PN visible (with Cdc42⁵) Cdc42¹ visible (with Cdc42¹) Cdc42⁵ reduced cell size \| somatic clone Cdc42³ cell shape defective Cdc42^dsRNA.cKa cell polarity \| maternal effect (with Cdc42⁶) Cdc42³ cell polarity \| maternal effect (with Cdc42³) Cdc42⁶ cell polarity, with Scer\GAL4^Pxn.PS, Scer\GAL4^crq.PA Cdc42^F89.Scer\UAS Cdc42^N17.Scer\UAS neuroanatomy defective Cdc42⁴ neuroanatomy defective, with Scer\GAL4^elav.PLu Cdc42^{V12.Scer\UAS.T:Hsap\MYC} neuroanatomy defective, with Scer\GAL4^Mz317 Cdc42^N17.Scer\UAS wound healing defective, with Scer\GAL4^en-e16E Cdc42^N17.Scer\UAS Phenotype manifest in Allele anterior crossvein \| ectopic Cdc42¹ follicle cell \| somatic clone Cdc42¹ eye (with Cdc42⁵) Cdc42¹ ommatidium (with Cdc42⁵) Cdc42¹ interommatidial bristle (with Cdc42⁵) Cdc42¹ anterior crossvein \| ectopic (with Cdc42⁵) Cdc42¹ wing (with Cdc42⁵) Cdc42¹ actin filament & rhabdomere \| somatic clone Cdc42² embryo \| maternal effect (with Cdc42⁶) Cdc42³ Cdc42⁴ dorsal closure embryo \| maternal effect (with Cdc42⁶) Cdc42³ embryonic epidermis \| maternal effect \| ventral (with Cdc42⁶) Cdc42³ embryonic/first instar larval cuticle \| maternal effect \| ventral (with Cdc42⁶) Cdc42³ embryo \| anterior \| maternal effect (with Cdc42⁶) Cdc42³ embryonic central nervous system \| maternal effect (with Cdc42⁶) Cdc42³ longitudinal connective \| maternal effect (with Cdc42⁶) Cdc42³ embryonic hemocyte (with Cdc42⁶) Cdc42³ ommatidium \| somatic clone Cdc42³ nurse cell & actin filament \| germ-line clone \| maternal effect Cdc42³ proventriculus inner layer primordium Cdc42³ proventriculus intermediate layer primordium Cdc42³ embryonic proventriculus Cdc42³ embryonic ganglionic branch Cdc42³ nurse cell ring canal \| germ-line clone \| maternal effect (with Cdc42⁶)