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Gene Dmel\Cks30A

General Information
Symbol	Dmel\Cks30A	Species	D. melanogaster
Name	Cyclin-dependent kinase subunit 30A	Annotation symbol	CG3738
Feature type	protein_coding_gene	FlyBase ID	FBgn0010314
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-32
Cytogenetic map	30A8-30A8	Sequence location	2L:9,330,767..9,331,981 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cyclin-dependent kinase subunit 30A is referred to in FlyBase by the symbol Cks30A (CG3738, FBgn0010314). It has the cytological map location 30A8. Its sequence location is 2L:9330767..9331981. Its molecular function is described as: cyclin-dependent protein kinase activity; protein binding; cyclin-dependent protein kinase regulator activity; protein serine/threonine kinase activity. It is involved in the biological processes: positive regulation of mitotic metaphase/anaphase transition; protein amino acid phosphorylation; cell cycle; histoblast morphogenesis; spindle assembly; syncytial blastoderm mitotic cell cycle; cyclin catabolic process; female meiosis. 7 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/first instar larval cuticle; aster; spindle pole centrosome; spindle. It has one annotated transcript and one annotated polypeptide.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	rem: remnants (T. Schupbach) Maternal effect lethal; eggs laid by females homozygous for rem1 show no visible sign of development when observed under transmitted light in stereomicroscope; eggs derived from rem2 often allow embryonic development; those embryos form fragmented cuticle with variable holes and head defects.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 30A8-30A8   Limits computationally determined from genome sequence between P{PZ}tai01351 and P{PZ}scat1&P{PZ}numb03235
Experimentally Determined Cytological Location
Cytogenetic map Notes References 30A-30A   (Swan, 2005.3.7) 30A7-30A8 (determined by in situ hybridisation)   (Celniker, 1998.11.30) 30A7-30A8 (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-32
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Cks30A for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cks30A-RA FBtr0079799  NM_057599   1143   74
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Cks30A-PA   FBpp0079399   9.1   74   9.35     NP_476947   AAF52763
Additional Polypeptide Data & Comments
Reported size (kD)	74 (aa) (Finley et al., 1996)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0010314
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cyclin-dependent kinase, regulatory subunit (IPR000789)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392173   AA540552   AC005125   AC092492   AE003624 AAF52763   AI532831   AW942557   AY061285 AAL28833   BI237740   BI243636   BI374140   CO183334   CO186081   CO196020   CO263434   CO292618   CO341797   EC051059   EC051570   EC056471   EC056626   EC076874   EC082212   EC208280   EC227529   EC229634   EC259790   U40077 AAB02189
UniProtKB/Swiss-Prot	Q24152
UniProtKB/TrEMBL
Maps to	DS06478 (BDGP Project Members, 1994-1999, FlyBase, 1992-, Celniker, 1998.11.30) FBcl0297505 (FlyBase, 1992-)
Does NOT map to
Identified with	LD20271 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation Cks30A[HG24] 2L:9,331,307..9,331,307 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9331307T pr_change=P61S|Cks30A-PA reported_pr_change=P61S (Swan et al., 2005) point mutation Cks30A[RA74] 2L:9,331,308..9,331,308 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C9331308T pr_change=P61L|Cks30A-PA reported_pr_change=P61L (Swan et al., 2005)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7010
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0010314 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0010314
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Cks30AHG24 Cks30AKO Cks30ARA74 lethal | maternal effect Cks30AHG24 Cks30AKO Cks30ARA74 lethal | embryonic stage | non-rescuable maternal effect | recessive Cks30AHG24 Cks30ARA74 Other Phenotypes Allele meiotic cell cycle defective Cks30AKO meiotic cell cycle defective | female limited Cks30AHG24 Cks30ARA74 meiotic cell cycle defective | female Cks30AKO mitotic cell cycle defective | embryonic stage Cks30AHG24 Cks30AKO Cks30ARA74 Sterility Allele female sterile | recessive Cks30AHG24 Cks30ARA74 male fertile Cks30AHG24 Cks30AKO Cks30ARA74 Phenotype manifest in Allele aster & oocyte Cks30AHG24 Cks30ARA74 larval cuticle & larval abdomen Cks30AHG24 Cks30AKO Cks30ARA74 embryonic/first instar larval cuticle Cks30AHG24 larval cuticle & larval abdomen (with Cks30ARA74) Cks30AHG24 Cks30AKO aster Cks30AKO spindle pole centrosome Cks30AKO spindle Cks30AKO larval cuticle & larval abdomen (with Cks30AKO) Cks30ARA74 larval cuticle & larval abdomen (with Cks30AHG24) Cks30ARA74
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Cks30A Class Mutagen Stocks Known lesion Cks30AHG24 ethyl methanesulfonate 0 Yes Cks30AKO recombination 0 Yes Cks30ARA74 ethyl methanesulfonate 1 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cks30A Class Mutagen Stocks Known lesion Cks30AGD13692 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Cks30AK10E.S50E.Scer\UAS.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | amino acid replacement 0 Yes Cks30AP61L.Scer\UAS.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion in vitro construct | amino acid replacement 0 Yes Cks30AScer\UAS.T:Zzzz\FLAG in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)N22-3
Disrupted in	Df(2L)30A-C (Schupbach and Wieschaus, 1989, Lane and Kalderon, 1993) Df(2L)TE2 (Lane and Kalderon, 1993) Df(2L)γ12 (Lane and Kalderon, 1993) Df(2L)γ29 (Lane and Kalderon, 1993) Df(2L)γ4 (Lane and Kalderon, 1993)
Not disrupted in	Df(2L)232 (Lane and Kalderon, 1993) Df(2L)50 (Lane and Kalderon, 1993) Df(2L)γ25 (Lane and Kalderon, 1993) Df(2L)γ7 (Lane and Kalderon, 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13692} NA P{UAS-Cks30A-FLAG} NA P{UAS-FLAG-Cks30A.K10E.S50E} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Mutations in Cks30A show a strict maternal effect phenotype with defects during presyncytial stages. (Schupbach and Wieschaus, 1989) The yeast interaction trap selection has been used to isolate cell cycle regulatory proteins that interact with cdc2 and cdc2c. Cks30A interacts with cdc2 and cdc2c. (Finley and Brent, 1993) Yeast two-hybrid system has been used to characterise Cyclin-dependent kinase interactor proteins (the prey) in hunts with either cdc2 or cdc2c as baits. Cks30A interacts with cdc2 cdc2c and CycJ, and other human and S.cerevisiae cyclin-dependent kinases to activate transcription of a reporter gene, Ecol\lacZ. (Finley and Brent, 1994) Isolated by interaction with cdc2 and cdc2c using the interaction trap two hybrid system. (Finley et al., 1996)
Gene Ontology: Function, Process & Cellular Component ( 12 )
Molecular Function
CV term Evidence References
cyclin-dependent protein kinase activity inferred from sequence or structural similarity (Finley and Brent, 1994)
cyclin-dependent protein kinase regulator activity inferred from sequence or structural similarity with UniProtKB:P56390 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
protein binding inferred from physical interaction with FLYBASE:cdc2; FB:FBgn0004106 AND inferred from physical interaction with FLYBASE:cdc2c; FB:FBgn0004107 (Finley et al., 1996)
protein serine/threonine kinase activity non-traceable author statement (Finley, 1995.11.4)
Biological Process
CV term Evidence References
cell cycle inferred from mutant phenotype (Swan et al., 2005)
cyclin catabolic process inferred from mutant phenotype (Swan and Schupbach, 2007)
female meiosis inferred from mutant phenotype (Swan and Schupbach, 2007)
histoblast morphogenesis inferred from mutant phenotype (Swan et al., 2005)
positive regulation of mitotic metaphase/anaphase transition non-traceable author statement (Swan and Schupbach, 2005)
protein amino acid phosphorylation inferred from sequence or structural similarity (Finley and Brent, 1994) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
spindle assembly inferred from mutant phenotype (Swan et al., 2005)
syncytial blastoderm mitotic cell cycle inferred from mutant phenotype (Swan et al., 2005)
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cks30A allele Gene References unspecified cort (Swan and Schupbach, 2007)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 60354
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22718 Dere\GG25340 Dgri\GH13319 Dmoj\GI12276 Dpse\GA25305 Dsec\GM17424 Dsim\GD23595 Dvir\GJ17855 Dyak\GE18832
Curated drosophilid orthologs	Dpse\GA17652
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000018659 Anopheles gambiae (Ensembl) ENSBTAP00000033878 Bos taurus (Ensembl) ENSCAFP00000025240 Canis familiaris (Ensembl) ENSCINP00000009144 Ciona intestinalis (Ensembl) NEWSINFRUP00000137450 Takifugu rubripes (Ensembl) ENSGALP00000017371 Gallus gallus (Ensembl) ENSP00000311083 Homo sapiens (Ensembl) ENSP00000317298 Homo sapiens (Ensembl) ENSP00000344538 Homo sapiens (Ensembl) ENSMMUP00000008659 Macaca mulatta (Ensembl) ENSMODP00000021379 Monodelphis domestica (Ensembl) ENSPTRP00000002352 Pan troglodytes (Ensembl) ENSRNOP00000048897 Rattus norvegicus (Ensembl) GSTENP00028772001 Tetraodon nigroviridis (Ensembl) ENSXETP00000033664 Xenopus tropicalis (Ensembl) AT2G27960.1 Arabidopsis thaliana (Arabidopsis) AT2G27970.1 Arabidopsis thaliana (Arabidopsis) WBGene00035798 Caenorhabditis briggsae (Wormbase) WBGene00001051 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040801-120 Danio rerio (Zfin) ZDB-GENE-040801-112 Danio rerio (Zfin) MGI:1889208 Mus musculus (Informatics) SPBC1734.14c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v35810 w1118; P{GD13692}v35810/CyO
Bloomington	5104 Cks30ARA74 cn1 bw1/CyO, l(2)DTS5131
Genomic Clones ( 1 )
	BACR20B09
cDNA Clones ( 16 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD20271
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE41337 SD04459 RE34025 RE61660 LD11077
Other clones	EK015636 EP13157 EK200609 EC25481 EK088518 EC051059 EC051570 EC056626 EC056471 EK000201
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0010314 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0010314
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: Cks30A Cks (Swan, 2005.3.7)
Source for database merge of
Additional comments	Source for merge: Cks30A rem (Swan et al., 2005)
Comments About Role
	Cks30A has a role in the metaphase to anaphase transition in female meiosis and in the mitotic divisions of the syncytial embryo. (Swan and Schupbach, 2005)
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057599 RefSeq (Proteins) • NP_476947 DDBJ / EMBL / GenBank • AA540552 AC005125 AC092492 AE003624 AAF52763 AW942557 AY061285 AAL28833 U40077 AAB02189 UniProtKB/Swiss-Prot • Q24152
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cyclin-dependent kinase, regulatory subunit (IPR000789)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 60354 DEDB - Drosophila exon database: splicing graphs • 7010 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0010314 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0010314 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0010314 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0010314 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0010314 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0010314 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000018659 Anopheles gambiae (Ensembl) ENSBTAP00000033878 Bos taurus (Ensembl) ENSCAFP00000025240 Canis familiaris (Ensembl) ENSCINP00000009144 Ciona intestinalis (Ensembl) NEWSINFRUP00000137450 Takifugu rubripes (Ensembl) ENSGALP00000017371 Gallus gallus (Ensembl) ENSP00000311083 Homo sapiens (Ensembl) ENSP00000317298 Homo sapiens (Ensembl) ENSP00000344538 Homo sapiens (Ensembl) ENSMMUP00000008659 Macaca mulatta (Ensembl) ENSMODP00000021379 Monodelphis domestica (Ensembl) ENSPTRP00000002352 Pan troglodytes (Ensembl) ENSRNOP00000048897 Rattus norvegicus (Ensembl) GSTENP00028772001 Tetraodon nigroviridis (Ensembl) ENSXETP00000033664 Xenopus tropicalis (Ensembl) AT2G27960.1 Arabidopsis thaliana (Arabidopsis) AT2G27970.1 Arabidopsis thaliana (Arabidopsis) WBGene00035798 Caenorhabditis briggsae (Wormbase) WBGene00001051 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040801-120 Danio rerio (Zfin) ZDB-GENE-040801-112 Danio rerio (Zfin) MGI:1889208 Mus musculus (Informatics) SPBC1734.14c Schizosaccharomyces pombe (Sanger) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/cks30.htm PANTHER - Protein classification by function, families, and pathways • FBgn0010314 University of Chicago Dev. Expression • FBgn0010314
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	Cdi2 (Breitwieser et al., 1996, Finley et al., 1996, ) CDI2 (Finley and Brent, 1993) cdi2   CG3738 (Liu et al., 2005) Cks (Ren et al., 2005, Asha et al., 2003, Giot et al., 2003, Finley and Brent, 1994, Hueber et al., 2007) cks (Gao et al., 2008) cks30A (Swan and Schupbach, 2005) Cks30A (Liu et al