Gene Dmel\TfIIFα

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General Information

Symbol

Dmel\TfIIFα

Species

D. melanogaster

Name

Transcription factor IIFα

Annotation symbol

CG10281

Feature type

protein_coding_gene

FlyBase ID

FBgn0010282

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

83E5-83E5

Sequence location

3R:2,206,181..2,208,333 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Transcription factor IIFα is referred to in FlyBase by the symbol TfIIFα (CG10281, FBgn0010282). It has the cytological map location 83E5. Its sequence location is 3R:2206181..2208333. Its molecular function is described as: general RNA polymerase II transcription factor activity; transcription initiation factor activity; DNA binding; catalytic activity; transcription activator activity. It is involved in the biological processes: transcription initiation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; positive regulation of transcription. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83E5-83E5

Limits computationally determined from genome sequence between P{lacW}cas^j1C2 and P{PZ}lab⁰¹²⁴¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\TfIIFα for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

TfIIFalpha-RA

FBtr0081719

NM_079522

1953

577

Additional Transcript Data & Comments

Reported size (kB)

1.929 (longest cDNA)

(Kephart et al., 1993)

Comments

TFIIFα was cloned from a Kc cell cDNA library by sequence similarity to human RAP74.

(Kephart et al., 1993)

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

TfIIFalpha-PA

FBpp0081216

64.5

577

8.45

NP_524246

AAF54125

Additional Polypeptide Data & Comments

Reported size (kD)

577 (aa)

(Kephart et al., 1993, Gong et al., 1993)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010282

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Transcription initiation factor IIF, alpha subunit (IPR008851)

Transcription Factor IIF, Rap30/Rap74, interaction (IPR011039)

Winged helix repressor DNA-binding (IPR011991)

TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles

•

T02173
T02243

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q05913

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8529

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA942112

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010282

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010282

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010282

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for TfIIFalpha

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for TfIIFα

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of TfIIFα	Class	Mutagen	Stocks	Known lesion
TfIIFα^d01485		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of TfIIFα	Class	Mutagen	Stocks	Known lesion
TfIIFα^GD4143		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)WIN11

Useful duplication

Dp(3;3)Tpl-T21

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{XP}TfIIFα^d01485

Related Comments

Please look at the allele reports for the complete phenotype data

Cloned by sequence similarity to human RAP74 protein.

(Kephart et al., 1993)

Drosophila TfIIFα cloned by low-stringency hybridisation to human TfIIFα cDNA.

(Gong et al., 1993)

Analysis of TfIIFα deletion constructs in vitro suggests that almost all of the protein is required for transcription initiation, while only the N-terminal region is required for stimulation of transcription elongation.

(Kephart et al., 1994)

TfIIF has been purified and shown to include two subunits.

(Austin and Biggin, 1996)

Gene Ontology: Function, Process & Cellular Component ( 10 )

Molecular Function

CV term

Evidence

References

DNA binding

inferred from electronic annotation with InterPro:IPR008851

(FlyBase Curators et al., 2004-)

catalytic activity

inferred from electronic annotation with InterPro:IPR011039

(FlyBase Curators et al., 2004-)

general RNA polymerase II transcription factor activity

inferred from sequence or structural similarity with HGNC_gene:GTF2F1; HGNC:4652; OMIM:189968 AND inferred from sequence or structural similarity with SGD_LOCUS:TFG1; SGD:S0003418

(Aoyagi and Wassarman, 2000)

non-traceable author statement

(Kephart, 1992.6.22)

non-traceable author statement

(Gong, 1993.4.26)

transcription activator activity

inferred from electronic annotation with InterPro:IPR008851

(FlyBase Curators et al., 2004-)

transcription initiation factor activity

non-traceable author statement

(Swiss-Prot Project Members, 1994.2.1)

Biological Process

CV term

Evidence

References

positive regulation of transcription

inferred from electronic annotation with InterPro:IPR008851

(FlyBase Curators et al., 2004-)

transcription from RNA polymerase II promoter

non-traceable author statement

(Swiss-Prot Project Members, 1994.2.1)

transcription initiation from RNA polymerase II promoter

inferred from sequence or structural similarity with HGNC_gene:GTF2F1; HGNC:4652; OMIM:189968 AND inferred from sequence or structural similarity with SGD_LOCUS:TFG1; SGD:S0003418

(Aoyagi and Wassarman, 2000)

Cellular Component

CV term

Evidence

References

nucleus

non-traceable author statement

(Swiss-Prot Project Members, 1994.2.1)

transcription factor TFIIF complex

inferred from sequence or structural similarity with HGNC_gene:GTF2F1; HGNC:4652; OMIM:189968 AND inferred from sequence or structural similarity with SGD_LOCUS:TFG1; SGD:S0003418

(Aoyagi and Wassarman, 2000)

non-traceable author statement

(Kephart, 1992.6.22)

non-traceable author statement

(Gong, 1993.4.26)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

TfIIFα allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65991

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18359

Dere\GG13412

Dgri\GH19532

Dmoj\GI23011

Dper\GL21747

Dpse\GA26384

Dsec\GM10893

Dsim\GD19872

Dvir\GJ24604

Dwil\GK12178

Dyak\GE10218

Curated drosophilid orthologs

Dpse\GA10217

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000022073
Anopheles gambiae (Ensembl)
ENSBTAP00000027988
Bos taurus (Ensembl)
ENSCAFP00000027562
Canis familiaris (Ensembl)
ENSCINP00000003594
Ciona intestinalis (Ensembl)
NEWSINFRUP00000179630
Takifugu rubripes (Ensembl)
ENSP00000245811
Homo sapiens (Ensembl)
ENSMMUP00000010376
Macaca mulatta (Ensembl)
ENSMODP00000002348
Monodelphis domestica (Ensembl)
ENSPTRP00000017625
Pan troglodytes (Ensembl)
GSTENP00023730001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000012286
Xenopus tropicalis (Ensembl)
Q53QT0
Oryza sativa (Gramene)
Q53QT1
Oryza sativa (Gramene)
Q8LMN8
Oryza sativa (Gramene)
AT4G12610.1
Arabidopsis thaliana (Arabidopsis)
WBGene00026468
Caenorhabditis briggsae (Wormbase)
WBGene00015296
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-1296
Danio rerio (Zfin)
ZDB-GENE-030131-4557
Danio rerio (Zfin)
DDB0205751
Dictyostelium discoideum (Dictybase)
MGI:1923848
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Harvard

P{XP}TfIIFα^d01485

VDRC

v51209

w¹¹¹⁸; P{GD4143}v51209

v51211

w¹¹¹⁸; P{GD4143}v51211

Genomic Clones ( 2 )

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones