Gene Dmel\Dsor1

Help

Close All Open All

General Information

Symbol

Dmel\Dsor1

Species

D. melanogaster

Name

Downstream of raf1

Annotation symbol

CG15793

Feature type

protein_coding_gene

FlyBase ID

FBgn0010269

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

1-27.6

Cytogenetic map

8D2-8D3

Sequence location

X:9,141,828..9,144,068 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Downstream of raf1 is referred to in FlyBase by the symbol Dsor1 (CG15793, FBgn0010269). It has the cytological map location 8D2-8D3. Its sequence location is X:9141828..9144068. Its molecular function is described as: MAP kinase kinase activity; receptor signaling protein serine/threonine kinase activity; MAP kinase activity; ATP binding; protein serine/threonine kinase activity. It is involved in the biological processes: border follicle cell migration; signal transduction; determination of anterior/posterior axis, embryo; MAPKKK cascade; protein amino acid phosphorylation; hemocyte differentiation; epidermal growth factor receptor signaling pathway; regulation of Toll signaling pathway; sevenless signaling pathway; torso signaling pathway. 30 alleles are reported. The phenotypes of these alleles are annotated with 17 unique terms, many of which group under: peripheral nervous system; nervous system; organ system; adult; sense organ; cell part; egg; imaginal precursor; adult segment; chorion; chorion; adult mesothoracic segment; portion of tissue. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8D2-8D3

Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912^EP912

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

8D-8D

(Nishida, 1992.11.27)

8D-8D

(determined by in situ hybridisation)

(Tsuda et al., 1993)

Experimentally Determined Recombination Data

Location

1-27.6

(Nishida, 1992.11.27)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: anon-8Da+ Dsor1+ In direction of increasing cytology: anon-8Db- Dsor1+

(Tsuda et al., 1993)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Dsor1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Dsor1-RA

FBtr0071313

NM_078543

1344

396

Additional Transcript Data & Comments

Reported size (kB)

2.0 (northern blot)

(Tsuda et al., 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Dsor1-PA

FBpp0071248

43.9

396

6.25

NP_511098

AAF46475

Additional Polypeptide Data & Comments

Reported size (kD)

393 (aa); 44 (kD)

(Tsuda et al., 1993)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010269

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, core (IPR000719)

Serine/threonine protein kinase (IPR002290)

Serine/threonine protein kinase, active site (IPR008271)

Protein kinase-like (IPR011009)

Protein kinase ATP binding, conserved site (IPR017441)

Serine/threonine protein kinase-related (IPR017442)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24324

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD41207

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

Dsor1[XS520]
X:9,143,588..9,143,593

comment=Reported as in frame deletion of M308 and A309 in FBrf0087493. Maps to M311 and A312 in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692).
evidence=experimental

(Karim et al., 1996)

point mutation

Dsor1[Su6]-1
X:9,142,628..9,142,628

comment=Reported as T52M in FBrf93573. Maps to T55M in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). Is one of two amino acid replacements in the mutant. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=C9142628T
pr_change=T55M|Dsor1-PA
reported_pr_change=T52M

(Lim et al., 1997)

point mutation

Dsor1[Su24]
X:9,142,657..9,142,657

comment=Reported as E62K in FBrf93573. Maps to E65K in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=G9142657A
pr_change=E65K|Dsor1-PA
reported_pr_change=E62K

(Lim et al., 1997)

point mutation

Dsor1[Su34B]
X:9,142,721..9,142,721

comment=Reported as D83V in FBrf73777. Maps to D86V in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=A9142721T
pr_change=D86V|Dsor1-PA
reported_pr_change=D83V

(Lu et al., 1994)

point mutation

Dsor1[Su4]
X:9,142,904..9,142,904

comment=Reported as G144D in FBrf93573. Maps to G147D in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron . The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=G9142904A
pr_change=G147D|Dsor1-PA
reported_pr_change=G144D

(Lim et al., 1997)

point mutation

Dsor1[Su12]
X:9,142,948..9,142,948

comment=Reported as M159L in FBrf93573. Maps to M162L in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=A9142948C
pr_change=M162L|Dsor1-PA
reported_pr_change=M159L

(Lim et al., 1997)

point mutation

Dsor1[Su6]-2
X:9,142,957..9,142,959

comment=Reported as M162A in FBrf93573. Maps to M165A in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron . The annotated version is supported by full length cDNA LD41207 (GB:AY058692). This represents one of two amino acid replacements in the mutant. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
pr_change=M165A|Dsor1-PA
reported_pr_change=M162A

(Lim et al., 1997)

point mutation

Dsor1[Su1]
X:9,143,047..9,143,047

comment=Reported as G192S in FBrf93573. Maps to G195S in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=G9143047A
pr_change=G195S|Dsor1-PA
reported_pr_change=G192S

(Lim et al., 1997)

point mutation

Dsor1[Su18]
X:9,143,097..9,143,097

comment=Reported as K208N in FBrf93573. Maps to K211N in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=G9143097Y
pr_change=K211N|Dsor1-PA
reported_pr_change=K208N

(Lim et al., 1997)

point mutation

Dsor1[Su5]
X:9,143,102..9,143,102

comment=Reported as S210N in FBrf93573. Maps to S213N in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=G9143102A
pr_change=S213N|Dsor1-PA
reported_pr_change=S210N

(Lim et al., 1997)

point mutation

Dsor1[Su7]
X:9,143,498..9,143,498

comment=Reported as P278A in FBrf93573. Maps to P281A in the CDS encoded by the reference sequence. The difference is due to the use of an upstream splice acceptor in an upstream intron. The annotated version is supported by full length cDNA LD41207 (GB:AY058692). The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.
evidence=experimental
na_change=C9143498G
pr_change=P281A|Dsor1-PA
reported_pr_change=P278A

(Lim et al., 1997)

rescue fragment

Dsor1[+t9]
X:9,138,172..9,147,378

linked_to=BamHI-SalI_rfrag

(Lu et al., 1994)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

(Tsuda et al., 1993)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010269

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010269

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010269

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

Dsor1^LH110

lethal | recessive

lethal | polyphasic

Dsor1^LH110

lethal | embryonic stage | recessive

Dsor1^LH110

lethal | adult stage | recessive

Dsor1^XS520

Other Phenotypes

Allele

wild-type

Dsor1^+t9

visible

Dsor1^XS520

visible | recessive

Dsor1^XS520

visible | dominant

Dsor1^XS520

Phenotype manifest in

Allele

egg chamber | maternal effect | somatic clone

Dsor1⁷⁵⁶

chorion | maternal effect | somatic clone

Dsor1⁷⁵⁶

imaginal disc | somatic clone

eye

embryonic dorsal epidermis

Dsor1^LH110

pigment cell

Dsor1^Su1

photoreceptor cell R7

ommatidium

eye photoreceptor cell

Dsor1^XS520

photoreceptor cell R1

Dsor1^XS520

photoreceptor cell R2

Dsor1^XS520

photoreceptor cell R3

Dsor1^XS520

photoreceptor cell R4

Dsor1^XS520

photoreceptor cell R5

Dsor1^XS520

photoreceptor cell R6

Dsor1^XS520

wing vein

Dsor1^XS520

dorsal appendage

Dsor1^ts1

Classical Alleles ( 22 )

For All Classical Alleles Show

Allele of Dsor1	Class	Mutagen	Stocks	Known lesion
Dsor1¹			0	--
Dsor1⁷⁵⁶		ethyl methanesulfonate	0	--
Dsor1^G42		ethyl methanesulfonate	1	--
Dsor1^G79		ethyl methanesulfonate	1	--
Dsor1^Gp158		Δ2-3	0	Yes
Dsor1^LF133			0	--
Dsor1^LH110	amorph, loss of function		1	--
Dsor1^MAPKK			0	--
Dsor1^S-1221		ethyl methanesulfonate	1	--
Dsor1^Su12			0	Yes
Dsor1^Su18			0	Yes
Dsor1^Su1	hypomorph, gain of function	ethyl methanesulfonate	0	Yes
Dsor1^Su24			0	Yes
Dsor1^Su34B		ethyl methanesulfonate	0	Yes
Dsor1^Su4			0	Yes
Dsor1^Su5			0	Yes
Dsor1^Su6			0	Yes
Dsor1^Su7			0	Yes
Dsor1^XS520	hypomorph	X ray	0	Yes
Dsor1^r1		ethyl methanesulfonate	0	--
Dsor1^r2		ethyl methanesulfonate	0	--
Dsor1^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dsor1	Mutagen	Stocks	Known lesion
Dsor1^+t9	in vitro construct \| genomic fragment	0	Yes
Dsor1^{2E.MtnA.T:Hsap\MYC}	in vitro construct \| site directed mutagenesis	0	Yes
Dsor1^D83V	in vitro construct \| amino acid replacement	0	Yes
Dsor1^{DA.MtnA.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion in vitro construct \| amino acid replacement	0	Yes
Dsor1^GD9123	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Dsor1^dsRNA.cCa	in vitro construct \| RNAi	0	Yes
Dsor1^ts1	in vitro construct \| amino acid replacement in vitro construct \| site directed mutagenesis	0	Yes
Dsor1^ts2	in vitro construct \| amino acid replacement in vitro construct \| site directed mutagenesis	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)10-70d

Df(1)9a4-5

Useful duplication

Dp(1;4)A17

Disrupted in

Df(1)10-70d

(Santamaria and Randsholt, 1995)

Df(1)BSC537

(Christensen et al., 2008.6.11)

Df(1)BSC538

(Christensen et al., 2008.6.11)

Df(1)Exel9049

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9123}

characterization construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{}Dsor1^Gp158

Related Comments

Please look at the allele reports for the complete phenotype data

Dsor1 and phl both play a role in the determination of the rate of proliferation. Maternally derived Dsor1 is required for development of the embryonic termini. Dsor1 is involved in both the positive and negative regulation of tll.

(Tsuda et al., 1993)

Down-regulation of bcd activity depends on the function of Dsor1. Dsor1 acts downstream of phl in the tor pathway and encodes a MAP-kinase kinase (MAPKK). Several clustered consensus sites for MAP kinase phosphorylation can be found in the bcd coding sequence.

(Dostatni et al., 1994)

Dsor1 acts downstream of phl in the DER pathway.

(Hsu and Perrimon, 1994)

Additional alleles of Dsor1 have been isolated in screen for lethal mutations that fail to complement Df(1)10-70d.

(Santamaria and Randsholt, 1995)

A screen to identify mutations affecting the Ras85D signalling pathway identified alleles of phl, Dsor1, rl, aop, βggt-I, mts, ksr and phyl.

(Therrien et al., 1995)

Expression of Dsor1 in lin-3, let-23 or lin-45 C.elegans mutants permits P5.p, P6.p and P7.p segments to adopt the vulval fates in each of the vulvaless backgrounds.

(Koga and Ohshima, 1995)

Studies of interaction between argos and members of the Ras/MAPK pathway demonstrate the argos gene product is a negative regulator of signal transduction that acts upstream of the Ras/MAPK cascade.

(Sawamoto et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 15 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441, InterPro:IPR017442

(FlyBase Curators et al., 2004-)

MAP kinase activity

non-traceable author statement

(Swiss-Prot Project Members, 1997.2.1)