Gene Dmel\α-Cat

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\α-Cat for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

α-Cat-RA

FBtr0070038

NM_079495

2977

917

Additional Transcript Data & Comments

Reported size (kB)

3.4 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

alpha-Cat-PA

FBpp0070037

102.4

917

6.35

NP_524219

AAF45466

Additional Polypeptide Data & Comments

Reported size (kD)

935 (aa); 110 (kD observed)

Comments

A 76 amino acid region of arm protein is necessary and sufficient for binding α-Cat protein; point mutations in this region abolish α-Cat binding in vitro. The N-terminal 258 amino acids of α-Cat interact with arm protein.

(Pai et al., 1996)

External Data

(BDGP Project Members, 2000-)

PANTHER - Protein classification by function, families, and pathways

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FBgn0010215

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Vinculin, conserved site (IPR000633)

Alpha-catenin (IPR001033)

Vinculin/alpha-catenin (IPR006077)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P35220

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD30423

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage | third instar

embryonic stage,pupal stage,adult stage

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 16

embryonic/larval midgut

embryonic stage | stage 11

ventral midline

larval stage | third instar

eye disc

embryonic stage | stage 16

epidermis

larval stage | third instar

ommatidial cluster

larval stage | third instar

ventral thoracic disc | restricted

embryonic stage | stage 16

embryonic/larval foregut

embryonic stage | stage 16

embryonic/larval peripheral nervous system

embryonic stage | >=cellular blastoderm

larval stage | third instar

embryonic/larval salivary gland duct

larval stage | third instar

morphogenetic furrow |anterior to

larval stage | third instar

morphogenetic furrow | posterior to | restricted

embryonic stage | stage 16

embryonic/larval hindgut

embryonic stage | >=cellular blastoderm

ectoderm

embryonic stage | stage 16

embryonic/larval central nervous system

Marker for

Subcellular Localization

CV Term

adherens junction

(Pai et al., 1996)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA950570

External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0010215

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0010215

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | partially, with Scer\GAL4^Act5C.PI

α-Cat^{Scer\UAS.P\T.cMa}

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of α-Cat	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of α-Cat	Mutagen	Stocks	Known lesion
α-Cat^GD8808	in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes
α-Cat^{Scer\UAS.P\T.cMa}	in vitro construct \| regulatory fusion	0	Yes
α-Cat^{Scer\UAS.T:Avic\GFP-sg}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
α-Cat^a.cMa	in vitro construct \| RNAi	0	Yes
α-Cat^cMa	in vitro construct \| RNAi	0	Yes
α-Cat^dsRNA.cYa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-α-Cat.T:GFP.sg}

P{UASp-α-Cat.M}

characterization construct

P{UAS-α-Cat::shg.N}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

α-Cat has been cloned and sequenced, and its expression pattern has been analysed.

(Orsulic and Peifer, 1996)

The α-Cat gene product is part of a membrane-associated complex that includes the arm and aagp gene products.

(Peifer, 1993)

The binding sites for shg and α-Cat are mapped on arm protein, these bind independently of each other to arm and binding of each is required for the function of adherens junctions.

Yeast two-hybrid system and in vivo assays have identified a 76 amino acid region of arm that is necessary and sufficient for binding α-Cat and also that the N-terminal 258 amino acids of α-Cat interact with arm.

(Pai et al., 1996)

Gene Ontology: Function, Process & Cellular Component ( 15 )

Molecular Function

CV term

Evidence

References

actin binding

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

cadherin binding

non-traceable author statement

(Swiss-Prot Project Members, 1994.2.1)

inferred from electronic annotation with InterPro:IPR001033

cytoskeletal protein binding

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

structural molecule activity

inferred from electronic annotation with InterPro:IPR000633, InterPro:IPR001033, InterPro:IPR006077

(Swiss-Prot Project Members, 1994.2.1)

Biological Process

CV term

Evidence

References

cell adhesion

non-traceable author statement

inferred from electronic annotation with InterPro:IPR000633, InterPro:IPR001033, InterPro:IPR006077

establishment and/or maintenance of cell polarity

cytoskeletal anchoring

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

non-traceable author statement

(Humbert et al., 2003)

oocyte localization during germarium-derived egg chamber formation

traceable author statement

(Deng and Lin, 2001)

Cellular Component

CV term

Evidence

References

actin cytoskeleton

inferred from electronic annotation with InterPro:IPR000633, InterPro:IPR001033, InterPro:IPR006077

cell-cell adherens junction

adherens junction

non-traceable author statement

(Humbert et al., 2003)

non-traceable author statement

(Harden, 2002)

catenin complex

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

inferred from direct assay

(Wang et al., 2006)

cell-substrate junction

non-traceable author statement

(Swiss-Prot Project Members, 1994.2.1)

spot adherens junction

non-traceable author statement

(Wedlich, 2002)

zonula adherens

non-traceable author statement

(Oda, 1993)

non-traceable author statement

(Muller, 2003)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

α-Cat allele

Gene

References

α-Cat^{Scer\UAS.P\T.cMa}

Rho1

(Magie et al., 2002)

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65744

Orthologs

Genome-wide drosophilid orthologs

Dana\GF20570

Dere\GG16297

Dgri\α-Cat

Dmoj\GI23967

Dper\GL21877

Dpse\GA22221

Dsec\GM22485

Dsim\GD12044

Dvir\GJ11153

Dwil\GK11940

Dyak\GE19475

Curated drosophilid orthologs

Dana\α-Cat

Dgri\α-Cat

Dmoj\α-Cat

Dper\α-Cat

Dpse\alpha-Cat

Dwil\α-Cat